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Citations for Addgene :
51 - 100 of 1435 citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The full length of ACE2 sequence from pCEP4-myc-ACE2 (a gift from Erik Procko, Addgene plasmid #141185) was cloned into pHR-CMV lentiviral expression vector (a gift from A ...
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bioRxiv - Microbiology 2021Quote: Stable ACE2 expressing HeLa cells (HeLa-ACE2) were generated by transducing HeLa-dCas cells with pLENTI_hACE2_HygR (Addgene, #155296) followed selection with hygromycin ...
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bioRxiv - Biophysics 2020Quote: ... overnight dialysis and subsequent Sumo-tag cleavage by human sumo protease (His-tagged SenP1; Addgene #16356) 44 were performed in 50 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: ... A plasmid containing the ACE2 gene (Addgene) was digested with Nhe I and Kpn I and the fragment was ligated into the similarly digested pLenti-III-HA vector plasmid ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.WPRE (Addgene 145842), or by an IRES-neomycinR cassette ...
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bioRxiv - Microbiology 2020Quote: The ACE2 expression plasmid (Addgene, plasmid #1786) was received in bacteria and purified with a maxiprep kit (Zymogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... For overexpressing ACE2 (Addgene, Appendix Table 2) in HPLFs ...
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bioRxiv - Bioengineering 2024Quote: ... ACE2 plasmid is from Addgene (Cat#1786). All the other plasmids used in this study are cloned by following the standard protocol of Infusion HD (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) kindly provided by Caroline Goujon (Addgene plasmid # 145842) (Rebendenne et al ...
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bioRxiv - Bioengineering 2021Quote: ... and Tspan12 were gifts from Jeremy Nathans and the human ACE2 clone (Shang et al., 2020) was obtained from Addgene (code 145033). Synthetic DNA fragments of engineered VH containing SARS-CoV-2 RBD (resi 333-527 ...
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bioRxiv - Biochemistry 2022Quote: Human Drp1 (Uniprot ID: O00429-4) with a C-terminal StrepII tag in pET15b (Addgene plasmid #174428) was expressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: HeLa cells were seeded onto glass coverslips and transfected with either myc-ACE2 (pCEP4-myc-ACE2 from Addgene Plasmid #141185), ACE2 (Daly et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... SNAIL1 (Addgene #16225[18]) and SLUG (Addgene #25696 ...
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bioRxiv - Microbiology 2020Quote: ... The ACE2 gene (obtained from Addgene [Catalog #: 1786]) was placed under the control of the human cytomegalovirus (hCMV ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.IRES-neo.WPRE (Addgene 145840), or by an IRES-hygromycin R cassette ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.IRES-hygro.WPRE (Addgene 145841), respectively ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 sgRNA plasmid or Cas9Bsd plasmid (Addgene #68343) (45 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and (RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE(MT129)(Addgene#145840). 293/hACE2 population were then maintained in culture medium containing G418 to obtain 293 cells that stably expressing recombinant hACE2 ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene). Full-length EGFP fused N-terminally to a nuclear localization signal (NLS ...
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bioRxiv - Biochemistry 2023Quote: GST-tag human HP1⍺ was a kind gift from Naoko Tanese (Addgene plasmid # 24074; http://n2t.net/addgene:24074; RRID:Addgene_24074). GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were co-transfected with pcDNA3.0 plasmids containing human CDC50A (NM_018247, N-terminal FLAG tag; RRID: Addgene_203694) and ATP10B variants (O94823.2 ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Microbiology 2020Quote: ... cDNA used to generate ACE2 positive cells was constructed as follows: the ACE2 PmeI cDNA fragment obtained from the plasmid hACE2 (Addgene; #1786) was cloned in pMD2iPuror opened in EcoRV.
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bioRxiv - Immunology 2021Quote: Recombinant HA (rHA) proteins were expressed using the pcDNA 3.1+ plasmid (Addgene, Watertown, MA). Each HA gene was truncated by removing the transmembrane (TM ...
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bioRxiv - Neuroscience 2022Quote: ... The recombinant AAV vector was pseudotyped with AAV5 capsid protein and packaged by Addgene.
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bioRxiv - Microbiology 2020Quote: ... a total of 240 million Huh7.5.1-Cas9-blast or Huh7.5.1-Cas9-blast+ACE2-IRES-TMPRSS2-hygro cells were transduced with lentivirus of the human GeCKO v2 library (Addgene, #1000000049, gift from Feng Zhang) at a moi of 0.4 and subsequently selected using puromycin and expanded for 7 days ...
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bioRxiv - Microbiology 2022Quote: ... Dimeric human ACE2-IgG1 (hACE2) was generated by transfecting Expi293 cells with pcDNA3-sACE2-WT(732)-IgG129 (Addgene plasmid #154104, gift from Erik Procko) using 1 mg mL-1 PEI and then purifying from the supernatant five days post-transfection using rProtein A Sepharose (GE ...
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bioRxiv - Biochemistry 2023Quote: Two vectors with C-terminal MBP (maltose-binding protein)-His6 Tag (2Cc-T; Addgene Plasmid #55209) or N-terminal His10-MBP Tag (2CT-10 ...
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bioRxiv - Microbiology 2021Quote: ... pRRL.sin.cPPT.SFFV/ACE2.WPRE has been described (48) (Addgene 145842). Flag-DDX42 and Flag-Firefly were amplified by PCR from the aforementioned LV plasmids and cloned into a NotI-XhoI-digested modified version of pCAGGS (49 ...
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bioRxiv - Microbiology 2020Quote: ... from the pcDNA3.1(-)-ACE2-Strep plasmid (Addgene, Catalog #: 1786), using the primers hACE2-strep-for ...
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bioRxiv - Physiology 2022Quote: ... The pTwist-EF1a carrying the SARS-CoV-2 E protein with 2xSTREP tags were obtained from Addgene. pCMV-rpHluorin-N1 was a kind gift from Dr ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression vectors of the full-length SARS-CoV-2 spike and the human serine protease TMPRSS2 with a C-terminal C9-tag (TETSQVAPA) were acquired from AddGene (Summit Pharmaceutical International ...
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bioRxiv - Biochemistry 2020Quote: ... H-HCF-1) and full length human THAP11 cDNA with carboxy-terminal FLAG tag (Plasmid #28020; F-THAP11) were obtained from Addgene. Vectors containing full length human THAP1 cDNA with carboxy-terminal 1X FLAG tag (THAP1-F ...
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bioRxiv - Molecular Biology 2020Quote: Human Drosha cDNA with a Flag-tag at the amino-terminus was cloned into pBABE-puro vector (Addgene plasmid#1764) for producing retrovirus of human Drosha wild type (WT) ...
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bioRxiv - Biochemistry 2021Quote: ... Pichia pastoris expression vector pPICZc carrying human β-actin fused with thymosin β4 and 6xHis-tag was a gift from Mohan Balasubramanian (Addgene #111146, RRID:Addgene_111146). β-Actin cDNA was mutated to introduce K50C and C374A for labeling with a cross-linking reagent ...
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bioRxiv - Cell Biology 2023Quote: The human sFLT1-HA plasmid construct created through Gibson cloning of human sFLT1 cDNA into a pcDNA3.1 vector containing a C-terminal HA tag (pcDNA3-ALK2-HA, Addgene 80870). Sequencing validation was performed through Genewiz ...
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bioRxiv - Biochemistry 2021Quote: The sfGFP-ACE2 construct was obtained from Addgene (Plasmid #145171), which was deposited by Erik Proco (University of Illinois ...
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bioRxiv - Microbiology 2020Quote: ACE2 small guide RNA was constructed into pSLQ1651 (Addgene #51024) (44 ...
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bioRxiv - Biochemistry 2021Quote: ... pCEP4-myc-ACE2 (gift from Erik Procko; Addgene plasmid #141185). Oligonucleotides for Gibson assembly and site-directed mutagenesis were obtained from IDT (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: The SNAP-tag gene was PCR amplified from pSNAP-tag(m) (Addgene #101135) and cloned into pVpr-IN.eGFP(Albanese et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 18 µg psPAX2 packaging plasmid (Addgene#35002 ...
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bioRxiv - Cell Biology 2021Quote: ... mEmerald-actin-C-18 (Addgene #53978), and mApple-paxillin-22 (Addgene #54935) ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the target proteins were dialyzed against PBS (pH 7.4) and the His6-SUMO-tag was removed by enzymatic cleavage using human SENP1 protease at 4°C overnight (Addgene #16356) (51) ...
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bioRxiv - Neuroscience 2020Quote: ... with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Full-length human KRAS4B G12C was ectopically expressed from the retroviral expression vector pBABE containing an N-terminal HA-tag (Addgene #58901). Viral particles were generated by transient transfection of each expression vector into HEK 293T cells using Fugene6 (Promega ...
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bioRxiv - Immunology 2020Quote: ... Human orfeome clone 10217) was gateway cloned into an attR-destination vector encoding an N-terminal FLAG tag (Addgene, Plasmid #18700), with the Gateway™ LR Clonase™ II Enzyme mix (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: Full-length human APLP1 gene with a Flag tag in the N-terminal was cloned from pCAX APLP1 (Addgene plasmid#30141) and the primers were shown in Key Resources Table (NheI-Flg-hAPLP1-F/XhoI-His-hAPLP1-R) ...
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bioRxiv - Molecular Biology 2020Quote: ... The GFP nanobody-Fc fusion construct (pGEMHE-vhhGFP4-hIgG1-Fc; Addgene #105576) was described previously (Clift et al. ...
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bioRxiv - Immunology 2020Quote: Plasmid encoding untagged ACE2 was a gift from Hyeryun Choe (Addgene plasmid # 1786 ...
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bioRxiv - Microbiology 2023Quote: ... and HNEpC cells were transduced with pLEX307-ACE2-blast (Addgene #158449) to generate A549ACE2 ...