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Citations for Addgene :
251 - 300 of 2782 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Cell Biology 2022Quote: Dynamin-1-mCherry and dynamin-1(K44A)-mCherry were made by replacing the GFP from WT Dyn1 pEGFP and K44A Dyn1 pEGFP (Addgene #34680 and #34681), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... human E2F5 and human DP1 were purchased from Addgene, whereas the ORF encoding human p130 from Origene.
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bioRxiv - Molecular Biology 2020Quote: ... ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425 ...
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bioRxiv - Cell Biology 2020Quote: ... The pShuttle mCherry-Tubulin adenoviral vector (Tub-WT) was purchased (Addgene; Plasmid # 2676842). Tubulin mutants have been generated using the pShuttle mCherry-Tubulin plasmid following recommendations of the Q5 site directed-mutagenesis kit from New England Biolab (#E0554) ...
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bioRxiv - Cell Biology 2020Quote: ... PG13-luc (WT p53 binding sites) was a gift from Bert Vogelstein (Addgene plasmid # 16442 ...
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bioRxiv - Cell Biology 2019Quote: pRK5-HA-Ubiquitin-WT was a gift from Ted Dawson (Addgene plasmid # 17608). Murine Jam-C-GFPout and Jam-C-GFPout ΔPDZ have been described previously27 ...
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bioRxiv - Cell Biology 2021Quote: The following plasmids were kind gifts: pCMV 3XFLAG-LC3A WT (Addgene plasmid #123089), pCMV 3XFLAG-LC3B WT (Addgene plasmid #123092) ...
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bioRxiv - Biochemistry 2022Quote: pT7-7 WT α-syn construct (Addgene, USA, gifted from Hilal Lashuel [34]), was transformed into BL21-Gold (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... pUHD Cdk1 WT HA was a gift from Greg Enders (Addgene plasmid # 27652). CK2-α and CDK1 were sub-cloned into pTriEx-4 vector ...
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bioRxiv - Developmental Biology 2020Quote: ... pRK5-HA-Ubiquitin-WT and pRK5-HA-Ubiquitin-K0 were purchased from Addgene.
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bioRxiv - Molecular Biology 2019Quote: ... Flag-PIAS4 WT plasmid was a gift from Ke Shuai (Addgene plasmid # 15208). RNF4-Myc-FLAG WT plasmid was purchased from OriGene (CAT# ...
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bioRxiv - Molecular Biology 2019Quote: ... HA-Ubiquitin WT plasmid was a gift from Ted Dawson (Addgene plasmid # 17608). HA-SUMO-1 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48966) ...
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bioRxiv - Cell Biology 2019Quote: ... - and pcDNA3-myc-FLNa WT - a gift from John Blenis (Addgene plasmid # 8982)(Woo et al. ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding p53-WT-EGFP was a gift from Tyler Jacks (Addgene #12091). All mutant constructs used in this study were generated from this construct either by a QuickChange II XL kit (Stratagene ...
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bioRxiv - Cell Biology 2021Quote: Mammalian expression plasmids used in this study were: pJ3-SHP1-WT (Addgene, 8572), pJ3-SHP1-D419A ...
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bioRxiv - Developmental Biology 2020Quote: ... Venus-iLID-CAAX and tgRFPt-SspB WT were obtained from Addgene (60411, 60415). pMT>Gal4 (Klueg et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplified full-length KEAP1 WT and the HA-Clover construct (Addgene plasmid #163336) were digested with the EcoRV and NotI enzymes (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-N1_MeCP2(WT) was a gift from Adrian Bird (Addgene plasmid #110186; RRID:Addgene_110186), GFP only vector was created by digesting pEGFP-N1_MeCP2(WT ...
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-N1_MeCP2(WT) was a gift from Adrian Bird (Addgene plasmid #110186; RRID:Addgene_110186), GFP only vector was created by digesting pEGFP-N1_MeCP2(WT ...
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bioRxiv - Bioengineering 2020Quote: ... VPR complex was derived from lenti-EF1a-dCas9-VPR-Puro (Addgene 99373, Ho et al., 2017) and modified to abolish BsmBI restriction sites ...
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bioRxiv - Neuroscience 2021Quote: ... pombe NatB acetylase complex (Johnson, Coulton, Geeves, & Mulvihill, 2010) using pNatB plasmid (pACYCduet-naa20-naa25, Addgene, #53613 ...
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bioRxiv - Molecular Biology 2023Quote: ... After an incubation of 1h at room temperature with a pA-Tn5 adapter complex (Addgene #124601), the Tn5 enzyme tagmentation reaction was performed by adding a buffer containing MgCl2 to the samples and incubating for 1h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Microbiology 2019Quote: ... GM-CSF and IL-4 were produced from HEK293 cells transduced with pAIP-hGMCSF-co (Addgene #74168) or pAIP-hIL4-co (Addgene #74169) ...
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bioRxiv - Microbiology 2019Quote: ... These cytokine-conditioned media were produced from HEK293 cells stably transduced with pAIP-hGMCSFco (Addgene no. 74168) or pAIP-hIL4-co (Addgene no ...
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bioRxiv - Cell Biology 2022Quote: ... we co-transfected HEK293-FT cells with plasmids of interest with VSVG (pMD2.G; Addgene plasmid 12259) and psPAX2 (Addgene plasmid 12260 ...
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bioRxiv - Cancer Biology 2021Quote: Cas9-expressing stable cell lines were produced by infection with the lentivirus encoding human codon-optimized Streptococcus pyogenes Cas9 protein (LentiV_Cas9_puro, Addgene plasmid # 108100) and subsequent selection with 1 μg/mL puromycin ...
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bioRxiv - Biochemistry 2022Quote: ... was made by ligating a PCR fragment encoding full-length human protein between ApaI and BamHI sites of pHis10-PS-SNAPf (75) (gift from Ron Vale; Addgene plasmid #78512 ...
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bioRxiv - Biophysics 2019Quote: Expression of N-terminally acetylated human αS and βS proteins was performed via co-expression with pNatB plasmid (Addgene #53613) in E ...
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bioRxiv - Cell Biology 2021Quote: ... and GFP-hRab31.dn3 (#1019) plasmids encoding GFP-labeled versions of the indicated human wild-type proteins were from Addgene (Cat# 49549 ...
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bioRxiv - Neuroscience 2023Quote: ... An AAV8 encoding the inhibitory designer receptor KORD fused to the fluorescent protein mCitrine under the control of the human synapsin promoter in a Cre-dependent manner was obtained from Addgene (AAV8-hSyn-dF-HA-KORD-IRES-mCitrine ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the Cre enzyme fused to the green fluorescent protein (GFP) under the control of the human synapsin promoter was obtained from Addgene (AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40 ...
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bioRxiv - Neuroscience 2022Quote: ... 250nl of an anterograde adeno-associated virus (AAV) vector expressing a green fluorescent protein under the hSyn (human synapsin) promoter (AAV5-hSyn-eGFP; Addgene) was injected into ACC to fluorescently mark the anatomical boundary of the claustrum (White et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 2.3 x 1013 vg/mL) or 200 nL/side AAV5-CMV-HI-eGFP-Cre-WPRE-SV4 (Addgene; ≥ 1 x 1013 vg/mL) was injected at a rate of 100 nL/min into the insula of Pdynlox/lox and Oprk1lox/lox mice ...
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bioRxiv - Molecular Biology 2024Quote: The HTLV-1 CA (Gag amino acids 132-349) was cloned into the 6×His SUMO bacterial expression plasmid (pHYRSF53, Addgene, Watertown, MA), with mutants created by using the Gibson assembly procedure ...
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bioRxiv - Microbiology 2023Quote: ... The human ANP32A 1-149 construct was a gift from Cynthia Wolberger (Addgene plasmid # 67241 ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...
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bioRxiv - Immunology 2022Quote: ... pVitro-hygro-N-myc-hVps34/Vps15-C-V5-his-plasmid (Addgene #24055), pEGFP-Atg14L (Addgene #21635 ...
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bioRxiv - Biochemistry 2022Quote: pQE-60 roGFP2-His was a gift from Tobias Dick (Addgene #65046)(50) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid for expression of EPS15-GFP-His was obtained from Addgene (#170860). EPS15D177S was generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... human KLF4 (RRID:Addgene_17219), human c-MYC (RRID:Addgene_17220 ...
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bioRxiv - Cell Biology 2020Quote: ... human SOX2 (RRID:Addgene_17218), human KLF4 (RRID:Addgene_17219) ...
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bioRxiv - Neuroscience 2022Quote: ... human DNAJB4 (Addgene) and DNAJB6 (Addgene ...