Labshake search
Citations for Addgene :
1 - 50 of 209 citations for Glutathione Colorimetric Detection Kit 200 Low Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... N-terminal His6 plus glutathione S-transferase (GST; RRID:Addgene_29707); N-terminal His6 plus small ubiquitin-like modifier (SUMO ...
-
bioRxiv - Biochemistry 2023Quote: OsKAI2 and the OsKAI2int mutant were independently cloned and expressed as GST (Glutathione-S-Transferase) fusion protein from the expression vector pCOOL (Addgene). BL21 (DE3 ...
-
bioRxiv - Genetics 2020Quote: ... low copy Tdp-43 expression plasmid pRS416-GAL1p-TDP43-YFP (URA3) (Addgene: 27447), high copy Tdp-43 expression plasmid pRS426-GAL1p-TDP43-GFP (URA3 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Inserts were cloned into a low copy pACYC184-derived empty vector (Addgene # 157879) between the HindIII and EcoRI restriction sites using NEBuilder HiFi DNA Assembly mix (New England Biolabs) ...
-
bioRxiv - Plant Biology 2024Quote: ... the low copy number pFA31 backbone (Addgene plasmid #162708; Flamholz et al., 2020) was modified into two terminal acceptors compatible with Loop Assembly (pFA-Odd and pFA-Even) ...
-
bioRxiv - Neuroscience 2022Quote: ... for astrocyte Ca2+ detection or AAV9.hSyn.GCaMP6f (3 × 1012 gc/ml, Addgene) for neuronal Ca2+ detection was mixed with the astrocyte-targeted CalEx encoding construct AAV2/5.GfaABC1D-HA-hPMCA2w/b (1.18 × 1013 gc/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... [14] and low-titer Cre (AAV9-hSyn-Cre-WPRE-hGH; Addgene #105553, MOI = 5×103 vg) AAVs on DIV 7 ...
-
bioRxiv - Neuroscience 2019Quote: ... 200 µg/µL pCMV-oG48 (Addgene 74288) (or pCMV-G in a subset of experiments ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 ng of hMbPylRS-4xU6M15 (Addgene, #105830) and 200 ng of the respective amber construct were premixed in 20 µL of DMEM ...
-
bioRxiv - Neuroscience 2023Quote: ... and 200 uL AAV5-hSyn-Flex-ChrimsonR (AddGene #62723-AAV5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 200 ng of pCMV-Tol2 (Addgene no. 31823) and 50 ng CMV-GFP ...
-
bioRxiv - Physiology 2024Quote: ... 200 ng pDisplay-gGRAB5HT2m-IRES-mCherryCAAX (Addgene, #208710), 200 ng pcDNA3-5-HT2A-P2A-GCaMP8m ...
-
bioRxiv - Neuroscience 2023Quote: ... method into pDONR221 entry vector and then recombined into a yeast low-copy number CEN destination vector (Addgene, 70) to obtain pAG413-promGPD-WDR47 (pSF634) ...
-
bioRxiv - Neuroscience 2021Quote: ... C57BL/6J mice received unilateral injections of AAVrg-CaMKIIα-mCherry (200 nl at 200 nl/min, titer 2×1013 vg/ml, #114469-AAVrg, Addgene, MA, USA) into the right main OB (0.5 mm lateral from midline ...
-
bioRxiv - Neuroscience 2019Quote: ... 200 nl concentrated AAV1.Syn.GCaMP6f.WPRE.SV40 (RRID:Addgene_100837, Penn Vector Core) was injected into the cortex 200-300 µm below the surface with glass capillary ...
-
bioRxiv - Genomics 2020Quote: ... 200 ng of CRISPR-KRAB-MeCP2 vector (Addgene 110821) and 50 ng of gRNA gblock (IDT ...
-
bioRxiv - Physiology 2024Quote: ... or 200 ng pDisplay-ATP1.0-IRES-mCherryCAAX (Addgene, #167582) was transfected to HEK293T cells in 24-well plates ...
-
bioRxiv - Cell Biology 2021Quote: ... and sequences with low off-target score were synthetised as oligonucleotides and cloned into a PX458 plasmid (Addgene plasmid #48138). gRNA sequences used can be found in Fig ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were infected at low viral titer with the pooled mouse CRISPR lentiviral library containing 78,637 gRNAs targeting 19,674 genes (Addgene #73633-LV). Infected cells were selected with puromycin (1 μg/ml ...
-
bioRxiv - Cell Biology 2020Quote: Identified gRNAs in organoids which survived under low Wnt conditions were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (a gift from Feng Zhang, Addgene #42230) and transfected into organoids and HEK293T cells with Lipofectamine LTX or Lipofectamine 2000 (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: Cas9-expressing HAP1 cells were generated by low multiplicity of infection transduction with lentivirus generated from lentiCas9-Blast74 (Addgene 52962) as described in the preceding paragraph ...
-
bioRxiv - Cell Biology 2023Quote: ... RAD52KO and RAD52WT cells were transduced at a low multiplicity of infection (MOI<0.3) using the BFP-tagged Human Improved Genome-wide Knockout CRIPSR Library (Addgene #67989) (Tzelepis et al ...
-
bioRxiv - Neuroscience 2020Quote: ... the control virus AAV5-CAG-Flex-tdTomato (200 nL, Addgene) was used to anterogradely label MCPO GABAergic axons ...
-
bioRxiv - Neuroscience 2021Quote: ... 200 nL of rAAV2-retro jGCaMP7s (~1e13 gc/mL, Addgene) was injected at a rate of 70 nL·min-1 ...
-
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... low passage HEK293T cells were co-transfected with the corresponding pInducer20-FLAG-SMS2 construct and the packaging vectors psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...
-
bioRxiv - Genetics 2020Quote: ... were transduced at a low multiplicity of infection (MOI) with virus containing sgRNA library cloned in lentiGuide-Puro (Addgene plasmid 52963) to ensure that most cells received only one sgRNA in expansion phase medium42 ...
-
bioRxiv - Cell Biology 2020Quote: ... the Cas9 and mCherry for detection of electroporated cells were obtained from Addgene (plasmids #66939, #66940, #66941). All targeting constructs (for both HDR and NHEJ ...
-
bioRxiv - Neuroscience 2021Quote: ... 100-200 nL of rAAV2-retro jGCaMP7s (~1e13 gc/mL, Addgene) was injected at a rate of 70 nL·min-1 using a Microinject system (World Precision Instruments) ...
-
bioRxiv - Neuroscience 2021Quote: ... 100-200 nl of AAV8-hSyn-DIO-hM4D(Gi)-mCherry (Addgene) were injected (DV −3.6 mm ...
-
bioRxiv - Neuroscience 2023Quote: ... and 200 nl of AAV1-CaMKII-GCaMP6f (Addgene, Catalog #100834-AAV1) was microinfused into the PrL cortex (AP ...
-
bioRxiv - Neuroscience 2022Quote: ... and 100-200 nl cre-dependent tdTomato (AAV2/1.FLEX.tdTomato.WPRE.SV40, Addgene), and some cases ...
-
bioRxiv - Immunology 2024Quote: ... 200 ng of envelope plasmid (Addgene #8454; courtesy of Bob Weinberg), and 6 of μL polyethyleneimine (PEI ...
-
bioRxiv - Neuroscience 2022Quote: ... two 200 nL injections of AAV.CAG.Flex.GCaMP6s.WPRE.SV40 (1.9 x 1013 GC/ml, Addgene), were performed in the LC of C57BL/6-Tg(Dbh-icre)1Gsc mice (Jax 4355551 ...
-
bioRxiv - Genomics 2024Quote: ... according to the manufacturers instructions with 200 ng pMD2.G (Addgene #12259), 600 ng psPAX2 (Addgene #12260 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 200 ng of pJC6-GL3 (#11979, Addgene; cJUN), which contains the cJUN promoter linked to the firefly luciferase gene ...
-
bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates (200 µg) were incubated with GST-RAF-RBD (Addgene) bound to glutathione agarose beads at 4°C for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... A genetically encoded calcium indicator for the detection of ACh activity (1 μL, AAV9-hSyn-ACh3.0; AddGene; Watertown, MA) was infused into the medial prefrontal cortex (AP(2.7mm) ...
-
bioRxiv - Cell Biology 2020Quote: ... and further into pAG423Gal-ccdB (high copy yeast expression plasmid) and pAG413Gal-ccdB (low copy yeast expression plasmid) vectors (Addgene plasmid #14149 and 14141) through standard Gateway Cloning protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... we then injected ~200 nL AAVretro-Ef1a-Cre (Salk vector core, Addgene #55637) into one site contralateral to the CN injections ...
-
bioRxiv - Physiology 2021Quote: ... 200 nL AAV-DIO-ChR2-mCherry (Addgene, 20297-AAV8, 1.9 × 101³ vg/ml) was injected unilaterally at 75 nL/min ...
-
bioRxiv - Molecular Biology 2020Quote: ... The annealed oligos were diluted (1:200) and cloned into lentiCRISPRv2 (Addgene # 52961) using a Golden Gate Assembly strategy (containing ...
-
bioRxiv - Neuroscience 2023Quote: ... 200 nL (in PIL) of either AAV8-hSyn-DIO-hM4D(Gi)-mCherry (Addgene, Item# 44362-AAV8 ...
-
bioRxiv - Neuroscience 2023Quote: ... 200 nl of AAV2-retro-hSyn-Cre (2.39E+13 gc/ml from Addgene) or RVΔL-5Cre(B19G ...
-
bioRxiv - Neuroscience 2022Quote: ... wildtype mice were injected with 100-200 nl AAV1-Syn-Cre (AAV.hSyn.Cre.WPRE.hGH, Addgene) in the posterior cerebellum (AP -7.2 ...
-
bioRxiv - Neuroscience 2023Quote: ... 200-500 nL of AAV5-Flex-ArchT-tdTomato (Addgene, 1.6×1013 vg /mL) was injected in the lateral SC at the same coordinates followed by implantation of an optical fiber over the injection site.
-
bioRxiv - Genomics 2020Quote: We constructed a vector (sgOpti-HyPR) capable of expressing both a gRNA and a “detection barcode” by modifying sgOpti (Addgene 85681) to insert a 400 bp fragment between the puromycin resistance cassette and WPRE ...
-
bioRxiv - Developmental Biology 2020Quote: ... New fasta and annotation files were created using mkref in mm10 for the detection of the H2B::GFP sequence (Addgene #11680). CellRanger count function was used to assign reads to genomic features ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The YTK kit (Addgene; Kit #1000000061) was used as a source for all relevant non-coding DNA sequences — including promoters and terminators ...
-
bioRxiv - Neuroscience 2021Quote: ... to inject 200–400 nl of AAV9-Flex-ChR2-tdTomato (~1011 viral particles; Addgene) 250-400 μm below the cortical surface (50 nl/min) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... MoClo Plant Parts Kit (Addgene Kit #1000000047), GreenGate Cloning System (Addgene Kit #1000000036 ...