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Citations for Addgene :
501 - 550 of 720 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Animals used for RSC imaging received a mixture of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (Addgene: 100837), AAV1.cFos-tTA and AAV1.TRE.mCherry at 20 to 120 nL/min into dorsal cortex using stereotactic coordinates −1.7 and −2.3 mm posterior to bregma ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth ...
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bioRxiv - Cancer Biology 2024Quote: HT29 cells previously infected with pLenti-U6-tdTomatato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and TdTomato were used ...
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bioRxiv - Neuroscience 2022Quote: ... Second generation lentiviral packaging plasmids psPAX and VSV-G envelope expressing plasmid pMD2.G (kind gifts from Didier Trono, Addgene plasmid #12260 and #12259) were used for the virus production ...
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bioRxiv - Genetics 2020Quote: Barcoded MSH2-2A-blR cDNA libraries were packaged into lentivirus by co-transfecting HEK293T/17 cells (ATCC) with the transfer plasmid pool plus envelope and packaging vectors (pMD2.G, Addgene #12259 and psPAX2, #12260). For each pool ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were bilaterally microinjected with 500 nL of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (purchased from Addgene 100837) at 20 -120 nL/min into the dorsal cortex using the stereotactic coordinates ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2020Quote: HT29 cells were infected with the pLenti-U6-tdTomato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and dTomato ...
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bioRxiv - Neuroscience 2021Quote: ... DV 1.4) before injecting 250 nL of virus (pGP-AAV1-syn-jGCaMP7f-SV40-WPRE; Addgene) in 50 nL pulses each interspersed by ∼10 s and the final pulse followed by a 10 min period where the needle was left in place to allow for diffusion ...
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bioRxiv - Neuroscience 2020Quote: ... We then injected the AAV5.CamKII.GCaMP6f.WPRE.SV40 virus (Addgene # 100834; 200 nL at 1 nl.s-1) in hippocampal CA1 using the following coordinates ...
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bioRxiv - Physiology 2020Quote: ... A retrograde AAV virus carrying a Cre gene (AAVrg-hSyn1-Cre, Addgene; 200 nl volume) was injected into the PBN (coordinates ...
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bioRxiv - Cancer Biology 2022Quote: MB-004 cells were transduced with the enti-dCas9-KRAB-T2A-GFP virus (Addgene # 71237). sgRNAs targeting SOX11/HNRNPH1-binding motifs in the distal enhancers were designed using the CHOPCHOP program (https://chopchop.cbu.uib.no) ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5µl of virus (either AAV1-CaMKIIa-ArchT-GFP or AAV8-CaMKIIa-GFP, Addgene, MA) was infused bilaterally with a 1 µl Hamilton syringe into either the PRC (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Anxa1-iCre mice (new line) were injected with AAV1-CAG-FLEX-GCaMP6f virus (RRID: Addgene_100835). For labelling VGlut2+ or Aldh1a1+ dopamine neurons ...
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bioRxiv - Neuroscience 2023Quote: ... the pipette containing AAVPHP.S-CAG-FLEX-tdTomato virus (Addgene 28306-PHP.S, 1.8×1013 vg/mL) targeted at 2 locations in the mandibular branch spaced 250 um apart ...
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bioRxiv - Neuroscience 2023Quote: ... USA) and [2] a Cre-dependent virus expressing green fluorescent protein (AAV-Flex-GFP; Addgene) or diphteria toxin (AAV2/9-EF1a-mCherry-Flex-dTA ...
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bioRxiv - Neuroscience 2023Quote: ... virus expressing tdTomato under the PVIN specific S5E2 enhancer (AAV9-S5E2-tdTomato, Addgene 135630-AAV9) was injected in mice using coordinates ...
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bioRxiv - Neuroscience 2023Quote: ... we infected organoids for 2 weeks with the AAV1-hSYN-eGFP virus (Addgene, 105539-AAV1), which directs expression of eGFP under the neuron-specific synapsin promoter ...
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bioRxiv - Neuroscience 2023Quote: Unilateral stereotaxic injections of AAV5-CaMKIIα-EGFP (Addgene #50469; virus titer ≥ 3×10¹² vg/mL) and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362 ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362; virus titer ≥ 7×10¹² vg/mL) viruses were performed on adult anesthetized (isoflurane ...
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bioRxiv - Cell Biology 2023Quote: ... at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G: Addgene 12259; pRSV-Rev: Addgene 12253; pMDLg/pRRE: Addgene 12251). 4.5μg of lentiviral donor plasmid was added to the mixture and incubated for 20 min with 10μL of polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2019Quote: ... Lck-GFP virus— pAAV.GfaABC1D.PI.Lck-GFP.SV40—was a gift from Baljit Khakh (Addgene viral prep # 105598-AAV5). AAV2/5 GfaABC1D.mCherry was obtained from Vector Biolabs ...
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bioRxiv - Immunology 2019Quote: ... 1 μl of Adeno-Associated Virus (AAV) (AAV2/1.CMV.HI.eGFP-Cre.WPRE.SV40, titer >=8E+12 vg/mL, Addgene) mixed with 0.5 μl of fast green (Sigma ...
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... Control animals were injected with a control virus (pAAV-CMV-PI-eGFP-WPRE-bGH; Addgene; #105530). For both experiments ...
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bioRxiv - Neuroscience 2020Quote: ... we injected ∼0.5 μl of anterograde trans-synaptic virus (AAV1-hsyn-Cre, Addgene Cat# 105553-AAV1) into the right DMS (bregma 0 mm ...
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bioRxiv - Neuroscience 2022Quote: ... then virus (AAV9-CaMKII-Cre, stock 2.1*1013 particles/nL, 1:1 dilution in PBS, Addgene) was pressure injected (NanoJect III ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (0.25-1 μl) containing either AAV5: hSyn-DIO-hM3Dq-mCherry (excitatory DREADD; Addgene, Watertown MA), AAV5 ...
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bioRxiv - Neuroscience 2020Quote: ... we drilled a small hole in the skull and injected ??nl of an AAV virus pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV9; from Addgene) using a glass pipette ...
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bioRxiv - Neuroscience 2020Quote: ... The virus pZac2.1 gfaABC1D-cyto-GCaMP6f was a gift from Baljit Khakh (Addgene plasmid cat.# 52925). Viral infections were performed at upto 20×109 viral particles as final concentration in 200µL volume per well ...
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bioRxiv - Neuroscience 2019Quote: ... AAV5 CaMKII-hM3Dq-IRES-mCitrine (3.75×10^14 or 3.75×10^13 virus molecules/mL) (Addgene plasmid #50466 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were infused with diluted (1:5) or undiluted virus pAAV5-CAMKIIa-(hChR2- H134R)-eYFP (Addgene-ChR2 titre ...
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bioRxiv - Neuroscience 2022Quote: We injected bilaterally 600μL channelrhodopsin-infused retrograde adeno associated virus pAAV-Syn-ChR2(H134R)-GFP (Addgene) in ventral hippocampus (A/P ...
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bioRxiv - Neuroscience 2022Quote: ... adeno-associated virus AAV8 carrying CaMKII-GCaMP6f (pENN.AAV.CamKII.GCaMP6f.WPRE.SV40. Addgene. #100834. 1×1012 genome copies per ml) was injected with Nanoject-III (Drummond Scientific Company ...
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bioRxiv - Neuroscience 2022Quote: ... We then used adeno-associated virus AAV1 carrying the construct GCaMP6s under the CaMKIIα promoter (Addgene# 107790-AAV9 ...
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bioRxiv - Physiology 2022Quote: ... The control virus AAV2/5-hSyn-DIOmCherry was ordered from Penn Vector Core (Addgene plasmid 50459). Animals were allowed to recover from stereotaxic surgery for a minimum of 21 days before initiation of any experiments ...
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bioRxiv - Neuroscience 2023Quote: ... we infected 4 DIV neurons with adeno-associated virus (AAV) expressing CamK2a-Cre (Addgene# 105558-AAV1) - pENN.AAV.CamKII 0.4.Cre.SV40 was a gift from James M ...
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bioRxiv - Neuroscience 2023Quote: ... 150-200nl of Cre- and Flp-dependent virus AAV8-hSyn-Con/Fon-EYFP (Addgene #55650-AAV8) was unilaterally injected into the MPN (AP 0 ...
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bioRxiv - Neuroscience 2023Quote: ... all mice received injections of a hM3Dq virus (pAAV-S5E2-Gq-P2A-dTomato; Addgene #135635-AAV1)58.
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bioRxiv - Neuroscience 2023Quote: ... unilateral injections of a virus designed to Cre-dependently express GCaMP6s (AAV1.Syn.Flex.GCaMP6s.WPRE.SV40, Addgene, 100845-AAV1) were performed in the arcuate nucleus of the hypothalamus (ARC ...
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bioRxiv - Neuroscience 2023Quote: ... with the rabies virus genomic vectors that contained either mCherry (pSADdeltaG-F3-mcherry, Addgene plasmid #32634), or GFP and Synaptophysin-RFP cDNA (pRVdG-N-P-M-EGFP-SynPhRFP-L ...
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bioRxiv - Neuroscience 2023Quote: ... 6-week-old Vglut2-Cre mice were injected with pAAV-EF1a-DIO-tdTomato-WPRE virus (RRID:Addgene_133786 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with plasmid pLXSN16E6E7 encoding the human papilloma virus HPVE6E7 gene (Addgene #52394) as described [18] ...
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bioRxiv - Neuroscience 2024Quote: ... we obtained the AAV2/5 virus of the GFAP -tdTomato vector from Addgene (#44332-AAV2/5). Titers ranged from 1-4 x 1013 vg/mL.
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bioRxiv - Evolutionary Biology 2020Quote: ... were generated using three plasmids to ensure a single round of infectivity: a pseudotyping plasmid for transient expression of the VSV-G envelope protein (pMD2.G, Addgene plasmid #12259, gift from Didier Trono), a plasmid for transient expression of the viral gag/pol ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253; pMDLg/pRRE: Addgene #12251, all gifts from Didier Trono) and 750ng of lentiviral transfer plasmid encoding the transgene of interest after a 15 minute incubation of these plasmids with 10μL of polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus was produced from pLenti-SV40-T-tsA58 (abm cat# LV629) as previously described (51) using the VSV envelope from pMD2.G (Addgene cat#12259, gift from Didier Trono) and the packaging plasmid pCMV delta R8.2 (Addgene cat # 12263 ...
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bioRxiv - Neuroscience 2021Quote: ... Univ of Pennsylvania) (Herman et al. 2016) and CaMKII-Cre virus (pENN-AAV9-CaMKII-Cre-SV40; Addgene, Watertown ...
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bioRxiv - Neuroscience 2021Quote: ... was loaded with the GCaMP6s virus (AAV9.Syn1.GCaMP6s.WPRE.SV40, ≈1.9x1013 GC/ml, Penn Vector Core; RRID: Addgene_100843) and was slowly lowered into VTA (DV -8.1 mm relative to brain surface) ...
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bioRxiv - Neuroscience 2022Quote: ... Circuit-specific viral expression was achieved using the retrograde properties of adeno-associated virus (AAV) serotype 5 23: AAV5.hSyn.HI.eGFP-Cre.WPRE.SV40 (Addgene; #105540). Sparse labelling was obtained using Cre-inducible ...