Labshake search
Citations for Addgene :
301 - 350 of 552 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... the DNA region encoding TFAM-mScarlet fusion was excised from the pcDNA3-TFAM-mScarlet plasmid (Addgene ref #129573) using BamHI and EcoRI endonucleases and inserted into the pWPXL plasmid (Addgene ref #12257) ...
-
bioRxiv - Molecular Biology 2023Quote: The DNA fragment encoding dLwaCas13a was PCR-amplified using pC035-dLwaCas13a-msfGFP (a gift from Feng Zhang; Addgene plasmid #91925 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The standard transfection mixture included 3 μg of total DNA [1.5 µg lentiviral plasmid + 1.5 µg helper plasmids - psPAX2 (RRID: Addgene_12260) and pMD2.G (RRID ...
-
bioRxiv - Neuroscience 2023Quote: ... For transfection, 5 µg of DNA (4:3:1 of a transgene, pCMVdR8.74 (packaging plasmid; Addgene, Plasmid #22036) and pMD2.G (envelope plasmid ...
-
bioRxiv - Molecular Biology 2023Quote: ... A recombinant DNA pcDNA3/Myc-DNMT1 was a gift from Arthur Riggs (Addgene plasmid #36939 Watertown, MA, USA) (Li et al. ...
-
bioRxiv - Genetics 2023Quote: ... inserts were either ordered as gBlocks from Integrated DNA Technologies (IDT) or amplified from pCMV-PE2 (Addgene #132775) or pCMV-PEmax (Addgene #174820 ...
-
bioRxiv - Neuroscience 2023Quote: ... Polycistronic CRISPR plasmids pX33075 and pX45876 were optimized so that one vector contained two U6 promotors with two sgRNA sequences.39 Guide RNA sequences were chosen as described.77,78 The DNA sequence encoding the TagRFP-T_A1aY1 Tyr-T sensor was amplified from Addgene plasmid #158751 (a gift from Minhajuddin Sirajuddin43 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The SNAP protein was amplified from the DNA-CARzeta-GFP plasmid (a gift from Ron Vale, Addgene # 89344). The monomeric streptavidin (mSA) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The DNA fragments of TPPP FL and FKBP were produced by PCR using existing templates and (Addgene #31184). The tagBFP gene was synthesized as gBlocks (Integrated DNA Technologies) ...
-
bioRxiv - Biophysics 2023Quote: ... total transfected DNA mass was kept at 10 ug for each condition using empty pBSK vector (Addgene # 212205). 500 ul Opti-MEM medium containing 20 ul Lipofectamine 3000 reagent was subsequently added to the plasmid containing mixture and incubated at RT for 15 mins ...
-
bioRxiv - Molecular Biology 2024Quote: The full length BRD4 DNA fragment was amplified by PCR from pcDNA4-TO-HA-BRD4FL (Addgene plasmid #31351)61 incorporated into linearized FM5 lentiviral vectors containing standardized linkers (generously provided by David Sanders ...
-
bioRxiv - Genomics 2023Quote: ... transfection kit and packaging plasmids pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
-
bioRxiv - Plant Biology 2024Quote: ... and terminators) from the GB2.0 kit purchased from Addgene (https://www.addgene.org/kits/orzaez-goldenbraid2/) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and TniQ were ordered from Integrated DNA Technologies (IDT) and cloned into bacterial expression plasmid pET28-MKH8SUMO (Addgene: #79526). The gene fragment for Cas12k was cloned into the bacterial expression plasmid pET-His6-StrepII-TEV LIC (Addgene ...
-
bioRxiv - Genomics 2022Quote: ... Per transfection reaction we used 7.5ul of 1 mg/mL polyethyleneimine (PEI) and 2.325 μg of total plasmid DNA (825 ng psPAX2: Addgene #12260; 550 ng pMD2.G: Addgene #12259 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Homology arms flanking the target site were amplified from genomic DNA and cloned into pBluescript II SK(+) (Addgene, 212205) by restriction-enzyme based cloning and the cHS4-CAG-nlstdTomato-cHS4 and cHS4-CAG-nlsGFP-cHS4constructs ...
-
bioRxiv - Immunology 2021Quote: ... The DNA of the constructs was mixed with the lentiviral envelope and packaging vectors pMD2-G and psPAX2c (Addgene) and polyethylenimine (PEI ...
-
bioRxiv - Cell Biology 2021Quote: ... and LT1 (8.75 µl) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene, 8454, 250 ng), psPAX2 (Addgene ...
-
bioRxiv - Biochemistry 2021Quote: ... genes encoding GDH2 and PEPCK were amplified from GS115 genomic DNA and cloned into pGHYB vector (Addgene plasmid #87447) (Yang et al. ...
-
bioRxiv - Microbiology 2020Quote: ... DNA encoding residues 1-177 of both human RAC1 and CDC42 were cloned into an unmodified pET-28a (Addgene) vector that encodes an N-terminal TEV-cleavable 6xHis-tag using the NdeI/XhoI sites ...
-
bioRxiv - Bioengineering 2022Quote: ... and LT1 (305 μL) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene 8454, 5 μg), psPAX2 (Addgene 12260 ...
-
bioRxiv - Plant Biology 2021Quote: ... pGY1-CA-RACB-K167R or pGY1 (empty) and 0.5 µg plasmid DNA coding for the transformation marker pUbi-GUSplus (a gift from Claudia Vickers, Addgene plasmid #64402 ...
-
bioRxiv - Genomics 2021Quote: ... and LT1 (8.25 µL) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene 8454, 250 ng), psPAX2 (Addgene 12260 ...
-
bioRxiv - Biochemistry 2021Quote: ... DNA encoding amino acids Ala696-Phe740 of human DDX1 was cloned into UC Berkeley MacroLab 438C (Addgene plasmid #55220). The resulting plasmid encoded for untagged RTCB(1-505) ...
-
bioRxiv - Immunology 2020Quote: ... The recombinant DNA encoding TCRMART-1 was synthesized by GeneScript (Nanjing, China) and ligated into pRRLSIN.cPPT.PGK vector (Addgene, 12252).
-
bioRxiv - Genetics 2020Quote: ... and LT1 (8.75 μL) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene 8454, 250 ng), psPAX2 (Addgene 12260 ...
-
bioRxiv - Biophysics 2022Quote: ... the DNA sequence encoding the T7 RNA polymerase promoter and sgRNA was amplified by PCR from pHelper_ShCAST_sgRNA vector (Addgene, #127921). The DNA template was then subjected to GeneJet PCR purification (ThermoScientific) ...
-
bioRxiv - Plant Biology 2022Quote: ... and HlWRKY1 was individually cloned into the bait vector pGBKT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) to produce fusion proteins containing a GAL4 activation domain (AD ...
-
bioRxiv - Synthetic Biology 2023Quote: ... a Platinum Gate TALEN assembly vector with this RVD, two DNA fragments were independently amplified with primer sets (RV_Fw1/Rv1, RV_Fw2/Rv2) and p1HD (Addgene #50664) as a PCR template ...
-
bioRxiv - Neuroscience 2023Quote: DNA plasmids encoding pENN-AAV-hSyn-Cre-hGH (#105553) and pAAV-CAG-H2B-GFP (#116869) were obtained from Addgene. AAV production was performed as previously described51 ...
-
bioRxiv - Genomics 2023Quote: ... and LT1 (8.25 μL) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene 8454, 125 ng), psPAX2 (Addgene 12260 ...
-
bioRxiv - Genomics 2022Quote: ... Each 15-cm2 plate was transfected with a total of 21 μg of plasmid DNA (1:2:1 ratio psPAX2-D64V:pLenti-STARR:pLAI-Env) [psPAX-D64V (Addgene #63586), and pLAI-HIV (Addgene #133996)] ...
-
bioRxiv - Cancer Biology 2022Quote: ... HEK293-FT cells at 70% confluence were co-transfected with the plasmid of interest (0.50 μg DNA/ reaction) and lentiviral packaging vectors (pMD2.G Addgene plasmid #12259 ...
-
The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... DNA encoding the indicated proteins were inserted between the attR recombination sites and shuttled into modified pLEX_307 vectors (Addgene) using Gateway technology (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... The transfection included 18ug of DNA per reaction and the plasmid (BMAL1-dLuc-GFP) to packaging vector (DVPR, Addgene) to envelope (VSV-G ...
-
bioRxiv - Genomics 2023Quote: ... and LT1 (8.25 μL) was combined with a DNA mixture of the packaging plasmid pCMV_VSVG (Addgene 8454, 125 ng), psPAX2 (Addgene 12260 ...
-
bioRxiv - Genomics 2024Quote: ... the 1 μg of total DNA was split in a 1:15 ratio of pCAG-NLS-Bxb1 (Addgene #51271) and recombination vector ...
-
bioRxiv - Cancer Biology 2019Quote: ... TALENs were assembled using the Golden Gate TALEN kit (Addgene) per the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... which was a gift from Paul Freemont (Addgene kit #1000000080). The Golden Gate digestion/ligation reactions and cycling were carried out as described by the kit (Moore et al ...
-
bioRxiv - Neuroscience 2020Quote: ... and constructs contained in the TALE Toolbox (Addgene kit # 1000000019), a gift from Feng Zhang ...
-
bioRxiv - Microbiology 2021Quote: ... along with sgRNA and other SapTrap-SEC kit plasmids (Addgene)61 ...
-
bioRxiv - Systems Biology 2022Quote: ... and lambda phosphatase were purchased from Addgene (Addgene Kit #1000000094)7.
-
bioRxiv - Microbiology 2020Quote: ... The MoClo Yeast Toolkit25 was obtained from Addgene (Kit # 1000000061) and was a gift from Prof ...
-
bioRxiv - Biochemistry 2021Quote: ... cerevisiae Advanced Gateway Destination Vector Kit were obtained from Addgene. Expand high fidelity PCR system (Roche Life Science ...
-
bioRxiv - Molecular Biology 2023Quote: ... which was a gift from John Dueber (Addgene kit # 1000000061). The assembly reaction conditions were 50 cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... which was a gift from John Dueber (Addgene kit # 1000000061). Note that the insert sequences were amplified in two separate fragments ...
-
bioRxiv - Cell Biology 2023Quote: ... TRUPATH was a gift from Bryan Roth (Addgene kit #1000000163) (27) ...
-
bioRxiv - Molecular Biology 2019Quote: ... were cloned via BpiI into pX330S-2 and pX330S-3 (Sakuma et al., 2014) and a third vector pGEP179_pX330K (this study) according to kit instructions (Addgene Kit#1000000055, Sakuma et al., 2014). The pGEP179_pX330K plasmid is a modified entry vector generated by cloning the BsaI-pU6-sgRNA-BsaI fragment from pX330A-1×3 (Sakuma et ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were transduced the next day with 1000 ng of plasmid DNA plus 250 ng pMD2.G (Cat# 12259, Addgene) and 750 ng psPax2 (Cat# 12260 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The double-stranded DNA of sgRNAs annealed from synthesized complimentary oligonucleotides were inserted into the pUC57-sgRNA vector (Addgene, 51132), and gRNAs were transcribed in vitro ...