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Citations for Addgene :
451 - 500 of 535 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The fragments are then cloned into a mammalian expression vector containing Flag and mEGFP (N- or C-terminal) (modified from Addgene #32104) using NEBuilder HiFi DNA Assembly kit (E2611) ...
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bioRxiv - Cell Biology 2023Quote: ... PH-FFAT/N-PH-FFAT sequences (5’-NheI/3’-AscI) were amplified by PCR from pLJM1-FLAG-GFP-OSBP plasmid (Addgene#134659). The sequences encoding Twitch (Twitch2b/Twitch7x/Twitch8x/Twitch9x ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with AAVS1-TRE3G-Mic10-FLAG-T2A-EGFP (Stephan et al., 2020, EMBOJ), AAVS1-TRE3G-DmMIC10-FLAG-T2A-EGFP, or AAVS1-TRE3G-EGFP (Qian et al., 2014) (Addgene 52343), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... LysoGFP-Sac1 and LysoGFP-Sac1 C389S were made by subcloning LysoGFP-Sac1 from pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT (Gift from Roberto Zoncu (Addgene #134645)) and pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS (Gift from Roberto Zoncu (Addgene #134653) ...
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bioRxiv - Neuroscience 2023Quote: ... A single vector CRISPR/Cas9 system was developed by replacing CMV-SP-PW-IRES-GFP with the hU6-Filler-EFS-SpCas9-FLAG-P2A-Puro cassette from lentiCRISPRv2 (Addgene #52961). To monitor the transduction rate ...
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bioRxiv - Cell Biology 2023Quote: U2OS T-REx FLAG-HA-FAM134s stable and inducible cell lines were infected with lentivirus carrying the pCW57-CMV-ssRFP-GFP-KDEL (Addgene #128257). We previously deleted the tetracycline response element ...
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bioRxiv - Neuroscience 2023Quote: ... the coding sequence of the extracellular region of human TfR1 (residues 89–760) were amplified by PCR and inserted into pCMV6-XL4 FLAG-NGRN-Fc (Addgene #115773) with EcoRV and XbaI sites ...
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bioRxiv - Immunology 2024Quote: 8 x 105 293T cells were seeded in 6-well plate and transfected with pcDNA3-FLAG-VSVG plasmids (Addgene, plasmid 80606) for 24 hours with 50 μl of purchased or previously collected VSVΔG-Luc pseudovirus (Kerafast ...
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bioRxiv - Microbiology 2024Quote: ... the cDNA of NSP1 were amplified from the Flag-NSP1 template and cloned into the lentiviral vector pWPI (Addgene, Cat# 12254). Plasmid expressing the human STAT2 mutant (HA-STAT2-F175A/R176A ...
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bioRxiv - Microbiology 2024Quote: ... The new product replaced an existing gene when inserted into pcDNA3.1 containing a triple N-FLAG-tag between KpnI and EcoRI (Addgene plasmid #67788). The GFP transfection control plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... The Myr-RSK3 vector (pWZL Neo Myr Flag RPS6KA2) was a kind gift from William Hahn & Jean Zhao (Addgene plasmid # 20621; (21)).
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bioRxiv - Cell Biology 2020Quote: For the generation of stable A549 cell lines expressing various PKCs and mutants we used following plasmids: pcDNA3-PKCε-Flag (wild-type, Addgene, Cambridge, MA), pcDNA3-Flag-K312PKCε ...
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bioRxiv - Cell Biology 2022Quote: ... a fragment encoding the residues 1382-1690 was cloned by PCR as a XhoI-NotI fragment from the pFRT/TO/FLAG/HA-DEST TNRC6C vector (Addgene plasmid 19885) (52 ...
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bioRxiv - Bioengineering 2019Quote: For functional experiments in H2170 knocked-in cells we used (FLAG) Snail 6SA (active Snail) plasmid which was a gift from Mien-Chie Hung (Addgene plasmid # 16221) [16] ...
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bioRxiv - Cancer Biology 2020Quote: ... The plenti-CAG-IKZF1-V1-FLAG-IRES-GFP plasmid used in the rescue experiments was a gift from William Kaelin (Addgene plasmid # 107387).29
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bioRxiv - Biophysics 2022Quote: ... media was refreshed with standard growth media and cells were co-transfected with SNAP-mGluR2 (no FLAG-tag) and chimeric G protein (Gqo5, Addgene plasmid #24500) (1:2 w/w ...
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bioRxiv - Microbiology 2022Quote: Lentivirus containing the MCMV m164 gene was produced by co-transfection of pLV-m164-FLAG with psPAX2 (a gift from Didier Trono, Addgene plasmid #12260) and pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 cell line stably expressing Flag–Rab40b-4A was created by cloning Rab40b-4A (primers purchased from IDT, Coralville, IA) into lentiviral pCS2-FLAG vector obtained from Addgene (Cambridge, MA). Cell lines were routinely tested for mycoplasma ...
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bioRxiv - Neuroscience 2019Quote: ... which contains the pore-forming subunit of the mitochondrial calcium uniporter coupled to GFP or a mito-mCherry construct generated by subcloning the targeting sequence of the pLYS1-FLAG-MitoGFP-HA plasmid into the mcherry2-N1 vector (Addgene plasmid # 54517). For Miro-1 knockdown ...
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bioRxiv - Neuroscience 2021Quote: ... AAV-hSyn1-nls-luciferase-FLAG (control) and AAV-hSyn1-Ptprt-ICD-FLAG were constructed from the backbone of AAV-hysn1-GCaMP6s-P2A-nls-dTomoto (Addgene gene, # 51084). AAV-hysn1-GCaMP6s-P2A-nls-dTomoto was digested with BamH1 and EconR1 ...
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bioRxiv - Cancer Biology 2021Quote: ... HA-mNcl and λNHA-mNcl were constructed by subcloning mNcl from pcDNA3-mNcl-FLAG into pCIneo-deltaHA and pCIneo-deltaNHA (gifts from W. Filipowicz; Addgene #115360, #115359) (Pillai et al. ...
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bioRxiv - Genomics 2020Quote: ... the 3×Flag-BUD13-6HIS fragment was transferred into the pLJM1 lentiviral construct using the NdeI and EcoRI sites (Addgene plasmid # 19319). We produced lentiviruses via co-transfection of pCMV-d8.91 ...
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bioRxiv - Microbiology 2020Quote: Constructs to express EBOV-VP24 and MUV-V fused to GFP were generated by PCR amplification from pCAGGS-FLAG-VP24 (kindly provided by Christopher Basler, Georgia State University) and MUV V-FLAG (a gift from Curt Horvath [16], Addgene plasmid #44908), and cloning into the pEGFP-C1 vector C-terminal to GFP (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... a Gibson assembly cloning strategy was performed using the XhoI and EcoRI restriction sites in our previously published lenti SYN-FLAG-dCas9-VPR backbone (Addgene plasmid #11419644), substituting VPR for KRAB-MeCP2 via PCR amplification from the original dCas9-KRAB-MeCP2 vector (a gift from Alejandro Chavez & George Church (Addgene plasmid #11082145)) ...
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bioRxiv - Neuroscience 2023Quote: ... A human Sirt3-RFP driven by the CaMKII promoter was constructed through PCR of the human Sirt3 sequence from Sirt3-FLAG (Addgene plasmid 13814)5 into the BamHI and AgeI sites of CaMKII-MICU3-RFP plasmid6 resulting in the linker sequence RPVVA joining Sirt3 and RFP sequences.
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bioRxiv - Immunology 2022Quote: ... The cDNA of FLAG-SLC29A3 was substituted with the BFP2Apuro sequence in the lentiviral pKLV-U6gRNA(BbsI)-PGKpuro2ABFP vector (Addgene plasmid 50946), excluding the U6gRNA(BbsI ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa or HEK293FT cells were co-transfected with the sgRNA plasmid and the pST1374-NLS-flag-linker-Cas9 (Addgene 44758, Xingxu Huang). After 24 h ...
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: Murine Taz was expressed by transfecting cells with pEF-TAZ-N-Flag from Michael Yaffe (Addgene #19025; RRID:Addgene_19025; Kanai et al., 2000).
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bioRxiv - Cell Biology 2023Quote: ... a donor plasmid pAAVS1-TRE3G-NGN2 was generated by replacing the EGFP sequence with N-terminal flag-tagged human NGN2 cDNA sequence in plasmid pAAVS1-TRE3G-EGFP (Addgene plasmid # 52343). 5μg of pAAVS1-TRE3G-NGN2 ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... pseudo-type lentiviruses coated with the SARS-CoV-2 S protein harboring the vector pCMV14-3X-Flag-SARS-CoV-2 S (a gift from Zhaohui Qian (Addgene plasmid #145780) were prepared by co-transfecting HEK293T cells with psPAX2 (a gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Developmental Biology 2023Quote: HEK293T cells were plated at a density of 2.8×105 cells in 6-well plates and transfected with MSCV-flag-PRDM16 (Addgene, 15504; RRID:MSCV PRDM16) and/or pCDNA3-NKX2-174 using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67, Addgene plasmid # 49386) followed by insertion into PCR linearised pRRL backbone ...
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bioRxiv - Biochemistry 2024Quote: The plasmid encoding amino acids 1-393 of the p53 protein with a FLAG tag at the C-terminus (Addgene plasmid #10838) was used as a template for site-directed mutagenesis to delete the N-terminal regions spanning amino acids 1-31 ...
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bioRxiv - Genetics 2024Quote: ... The Lenti-iCas9-Neo vector containing the Flag-iCas9-P2A-GFP cassette for Cas9 expression in K562 cells was obtained as a gift from Qin Yan (Addgene plasmid #85400,35). For Cas9 expression in HEK293T cells ...
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bioRxiv - Plant Biology 2021Quote: ... Gel-purified PCR product and SspI-digested pET-His6-MBP-TEV-LIC vector (Addgene) were treated with T4 DNA polymerase with 25 mM dCTP and dGTP for 30 min at 22°C followed by heat inactivation ...
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bioRxiv - Cell Biology 2022Quote: ... the retroviral FLAG-5SA-YAP and FLAG-5SA-S94A-YAP plasmids were generated via restriction-based cloning from pQCXIH-Myc-YAP-5SA (Addgene plasmid #33093 36 and pCMV-Flag-YAP-5SA/S94A (Addgene plasmid #33103 42, respectively, and pBABE-PURO (Addgene plasmid #1764 59). The CCDEL deletion was introduced in the latter via overlapping PCR with the primers YAP-DEL-CC-HA-F and YAP-DEL-CC-HA-R as described in 31 ...
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bioRxiv - Cell Biology 2023Quote: ... and EBP50-PDZ12 (aa 1-298) cloned into the pCMV-2-FLAG vector were gifts from Maria-Magdalena Georgescu (Addgene plasmids #28291-28297).
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bioRxiv - Cell Biology 2022Quote: Here the SmBiT was cloned in frame at the C-terminus of the GLP-1R and the GIPR by substitution of the Tango sequence on FLAG-tagged GLP-1R-Tango or GIPR-Tango (a gift from Prof Bryan Roth, University of North Carolina, USA; Addgene plasmids #66291 and #66294), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... N-terminal FLAG-tagged CAPS was created by recombining the human CAPS gene (CAPS/pENTR; DNAsu HscD00514950) into pEZFLAG (Addgene#18700; Guo et al., 2008) using Gateway cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... we gel extracted the 3x polyA sequence present on plasmid pENTR5’_ubi:loxP-EGFP-loxP (Addgene # 27322) and inserted it in the NotI-dephosphorylated p5E-UBI_Lflox_BFP plasmid through T4 ligation ...
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bioRxiv - Cell Biology 2024Quote: To generate RRBP1 KO HEK293T cells expressing EGFP-FLAG-APEX2-ePTS1 single gRNA transcriptional cassette prepared by PCR and pCAS9-mCherry empty (Addgene, 80975 (Schmid- Burgk et al., 2016)) were co-transfected into HEK293T cells expressing EGFP-FLAG-APEX2- ePTS1 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel-purified and cloned into EcoRV-cut pLenti CMV Puro DEST (w118-1) (Addgene #17452 ...
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bioRxiv - Cancer Biology 2022Quote: ... After PCR the insert was gel purified (GeneJet) and Gibson cloned into BsmBI-digested pXPR_053 (Addgene# 113591). Gibson cloned insert and vector was column purified (GeneJet ...
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bioRxiv - Genomics 2023Quote: ... sgRNAs were inserted into the BsmBI-digested and gel-purified CROPseq-Guide-Puro backbone (Addgene ID: 86708) with Gibson Assembly in the ratio of 2 ug sgRNA PCR product and 3 ug digested plasmid backbone ...
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bioRxiv - Immunology 2022Quote: ... target cells were derived by transfection with plasmids designed to express the SARS-CoV-2 D614 Spike protein with a c-terminus flag tag (kindly provided by Dr. Farzan, Addgene plasmid no. 156420 (Zhang et al., 2020)) ...
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bioRxiv - Cell Biology 2020Quote: ... Fragments were purified by agarose gel electrophoresis and individually subcloned into BamHI/NotI-digested pBluescript II KS+ (Addgene). Colonies positive for the plasmid were selected on LB agar with 100 μg/ml ampicillin (Gibco from Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... the biRhoBASTn cassette was gel purified and ligated into XhoI-digested and dephosphorylated mAzurite-C1 (Addgene Plasmid #54583) to yield mAzurite-biRhoBASTn ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA FB-SNAP and anti-HA FB-Halo (Addgene #129592), cut by EcoRI and combined with anti-FLAG frankenbody gblocks by Gibson assembly ...
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bioRxiv - Cell Biology 2019Quote: ... The PCR product was purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1765 ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product is purified from an agarose gel and inserted into pBABE-hygro (pBABE-hygro is a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid #1765 ...