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Citations for Addgene :
1 - 50 of 1030 citations for Acetylcholinesterase Fluorescent Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... USA) and [2] a Cre-dependent virus expressing green fluorescent protein (AAV-Flex-GFP; Addgene) or diphteria toxin (AAV2/9-EF1a-mCherry-Flex-dTA ...
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bioRxiv - Cell Biology 2020Quote: ... tandem-fluorescent LC3 (Addgene), GFP-EB1 (Addgene) ...
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bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...
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bioRxiv - Cell Biology 2023Quote: ... We generated monoclonal U2OS cell lines expressing the following fluorescent plasmids: 1) ptfLC3B was a gift from Tamotsu Yoshimori (Addgene plasmid #21074; http://n2t.net/addgene:21074; RRID:Addgene_2107443; 2) pMXs-puro GFP-DFCP1 was a gift from Noboru Mizushima (Addgene plasmid #38269 ...
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bioRxiv - Genetics 2019Quote: ... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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Hippocampal efferents to retrosplenial cortex and lateral septum are required for memory acquisitionbioRxiv - Neuroscience 2020Quote: ... enhanced yellow fluorescent protein (eYFP) or enhanced green fluorescent protein (eGFP): AAV1.CaMKIIa.eNpHR3.0.eYFP.WPRE.hGH (Addgene, #26971), AAV1.CaMKII.eYFP ...
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bioRxiv - Microbiology 2021Quote: ... 2 x 105 cells were seeded on 12-well plates and transiently transfected with 2 μg of plasmids encoding SARS-CoV-2 N (Addgene # 141391) or eGFP (Addgene # 141395 ...
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bioRxiv - Microbiology 2020Quote: Cas9 activity was tested by transducing pXPR_011 (Addgene #59702), which expresses both GFP and sgRNA against GFP ...
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bioRxiv - Cell Biology 2020Quote: ... or a fluorescent protein (pCDNA3-GFP; Addgene plasmid #74165 or mCherry2-N1 ...
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bioRxiv - Molecular Biology 2024Quote: Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library (Addgene#1000000096) was amplified in E coli ...
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bioRxiv - Neuroscience 2022Quote: ... and the fluorescent marker hSYN-eGFP (Addgene: 50465) plasmids for downstream creation of the probe for Synaptic Digestion (pSynDig ...
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bioRxiv - Synthetic Biology 2021Quote: ... and pBAD expressing fluorescent protein variants (Addgene, UK). Primers used in the assembly of the vectors are listed in SI Table 1.
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bioRxiv - Molecular Biology 2021Quote: ... iRFP670 fluorescent reporter vector (piRFP670-N1; Addgene#79987) and pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene #67974 ...
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bioRxiv - Biophysics 2022Quote: Fluorescent proteins were obtained from Addgene (https://www.addgene.org/) transfected using 0.2 ug plasmid DNA ...
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bioRxiv - Biochemistry 2020Quote: ... aureus with improved activity (eSrtA) was obtained from Addgene (plasmid #75144) and expressed in LB medium at 18°C for 16 hours (Chen et al. ...
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bioRxiv - Cell Biology 2019Quote: Reporters of enhancer activity were constructed by cloning mCerulean3 (Addgene #54730) into the multiple cloning site of Open Biosystems vector PB533A-2 ...
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Interleukin 4 controls the role of macrophages in pulmonary metastatic tumor cell seeding and growthbioRxiv - Cancer Biology 2021Quote: ... The fluorescent E0771-LG cell line expressing Clover (Addgene) [57] used in intravital experiments was established by standard transfection ...
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bioRxiv - Genomics 2023Quote: ... Cas9 activity was evaluated using the fluorescence- based reporter pXPR011 (Addgene #59702), which expressed GFP and cognate sgRNA to assess GFP knockdown upon successful CRISPR activity ...
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bioRxiv - Immunology 2020Quote: Plasmids containing fluorescent protein coding sequences mCerulean3-N1 (Addgene # 54730), mAzurite-N1 (Addgene # 54617) ...
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bioRxiv - Biochemistry 2021Quote: ... or N-terminal His6 plus green fluorescent protein (GFP; RRID:Addgene_29716). Note that in the plasmid names for this clone the numbering of Syx residues was based on the Syx isoform from NCBI Reference Sequence NP_001036128.1 ...
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bioRxiv - Genetics 2020Quote: ... and a blue fluorescent protein (BFP) expression cassette (Addgene #36086). The 1kb upstream and 1kb downstream arms were amplified from purified mouse genome from AB2.2 ES cells (ATCC #SCRC-1023 ...
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bioRxiv - Cell Biology 2023Quote: ... a monomeric red fluorescent protein (RFP)-FKBP12 (Addgene, Plasmid #67514) (71 ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: Plasmids encoding fluorescent fusion protein were either purchased from Addgene or constructed in house using ligation (NEB Cat# M2200S ...
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bioRxiv - Cell Biology 2023Quote: ... and enhanced green fluorescent protein (eGFP) (Addgene, Boston, MA, USA). Cells were nucleofected using the Amaxa 4D-Nucleofector (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: Cells were assessed for Cas9 activity using the pXPR_011 plasmid (Addgene plasmid # 59702)[63] according to protocol by Doench et al ...
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bioRxiv - Bioengineering 2021Quote: ... and screened for PE activity by transfecting CTT-inserting pegRNA alone (Addgene #132778) and sequencing the HEK3-target loci.
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bioRxiv - Physiology 2022Quote: ... PPARγ2 transcriptional activity was determined using the PPREx3-TK-luc reporter (Addgene, #1015).
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bioRxiv - Neuroscience 2023Quote: For pan-neuronal activity imaging 400nl of AAV1-Syn-GCaMP6s (Addgene #100843-AAV1) was injected unilaterally into the MPN of FVB/NJ mice ...
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bioRxiv - Synthetic Biology 2020Quote: ... and a C’-terminal yellow fluorescent protein (YFP; pICSL50005, Addgene #117536), and AtuOCS terminator ...
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bioRxiv - Genetics 2020Quote: ... The AAVS1 targeting fluorescent reporter #208 system was modified from Addgene ID 60431 ...
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bioRxiv - Neuroscience 2020Quote: ... carrying the fluorescent calcium indicator GCaMP6f (AAV.Syn.Flex.GCaMP6f.WPRE.SV40, 1E13 particles/ml, Addgene) into the left VTA (AP ...
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bioRxiv - Biochemistry 2022Quote: ... Mitochondria mCherry fluorescent-labeled marker (mCherry-Mito) was obtained from Addgene plasmid repository #55146 ...
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bioRxiv - Neuroscience 2022Quote: ... which would lead to increased activity in projection neurons of the cortex (Addgene, 50474).
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bioRxiv - Cancer Biology 2024Quote: ... DHB-mVenus (CDK2 activity reporter) was a gift from Tobias Meyer & Sabrina Spencer (Addgene plasmid # 136461 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 x 105 HeLa cells were seeded into a 6-well plate and next day transfected with 2 μg of the FLPe recombinase plasmid (Addgene #20733) (Beard et al. ...
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bioRxiv - Systems Biology 2019Quote: ... 350k HAP1 WT cells were seeded into a 6-well plate and 24 hours later cells were transfected with a mix of 2 µg pX459 plasmid (Addgene #62988) carrying a gRNA ...
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bioRxiv - Cell Biology 2019Quote: The fluorescent Gal3 reporter was PCR amplified from pEGFP-hGal3 (Addgene #73080), a gift from Tamotsu Yoshimori ...
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bioRxiv - Cell Biology 2019Quote: ... RCC1 was targeted with an infra-red fluorescent protein (IFP2.0 Addgene #54785), TIR1 was targeted with 9 Myc-tag sequences ...
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bioRxiv - Molecular Biology 2022Quote: For validation experiments we introduced the fluorescent protein EBFP2 (source: Addgene 54665) driven by the dpse enhancer and the CG13116 promoter in the RD-seq plasmid backbone to be able to gate for transfected cells in flow cytometry (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... The fluorescent reporter open-reading frames (S3 table) were ordered from Addgene and cloned into the accepting vectors utilizing PCR and Gibson assembly ...
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bioRxiv - Neuroscience 2024Quote: Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was commercially purchased (Addgene). The fluorescent protein mKate was inserted into a pcDNA3.1(- ...
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bioRxiv - Microbiology 2020Quote: ... Cas9 activity was checked using the XPR_047 assay (a gift from David Root, Addgene 107145) and was 79.5% and >83.4% ...
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bioRxiv - Biochemistry 2022Quote: Notch activity reporter (CBF:H2B-Venus) was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid #44211) (Nowotschin et al. ...
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bioRxiv - Physiology 2023Quote: Circadian clock gene activity was recorded using the vectors pLV6-Bmal-luc (Addgene plasmid #68833) and pLV6-Per2-luc ...
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bioRxiv - Systems Biology 2024Quote: ... pPBbsr-JNKKTR-mCherry plasmid containing JNK activity reporter (JNK-KTR) was obtained from Addgene (#115493). mCherry in this plasmid was exchanged with iRFP713 (from Addgene #111510 ...
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bioRxiv - Neuroscience 2024Quote: The YAP-dependent transcriptional activity was assessed using an 8xGTIIC-luciferase construct (Addgene plasmid # 34615). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Biochemistry 2023Quote: ... MEFs were split into 6 well plates to ∼80% confluence and transfected with 2 µg SV40 1: pBSSVD2005 (a gift from David Ron; Addgene plasmid #21826) using FuGENE HD according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Green fluorescent protein (GFP) from the Gateway entry vector pENTR4-GFP-C1 (Addgene) was transferred to pYES-DEST52 and pAG426GAL-ccdb using Gateway LR Clonase ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with tandem mRFP-GFP fluorescent-tagged LC3 (ptfLC3) (Addgene #21074). 24h post-transfection ...