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Citations for Addgene :
401 - 450 of 1336 citations for APRIL Human HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272 ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... coli and subcloned in-line with his-tagged MBP construct (2CT-10 vector, Addgene plasmid #55209). Recombinant MBP-EndoH fusion was expressed in and purified from E ...
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bioRxiv - Cancer Biology 2023Quote: SULF2 ORF including C-terminal Myc-His tag was subcloned from its source vector (Addgene # 13003) to lentiviral transfer vector pHR-CMV-TetO2_3C-Twin-Strep (Addgene # 113883 ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-human β-catenin (Addgene #24193), and NLS-mCherry (Addgene #49313 ...
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bioRxiv - Molecular Biology 2022Quote: ... pscALPSpuro-HsACE2 (human) (Addgene, MA, USA) were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... human a-SYN (A53T mutant) (Addgene), and mt-Keima (Addgene) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Cancer Biology 2024Quote: ORF encoding human MARK2 (Addgene, 23404) was cloned into pFL system with an N-terminal Strep2SUMO tag ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral particles carrying a luciferase reporter were produced at the EPFL Gene Therapy Facility by transfecting HEK293 cells with the pHIV-Luc-ZsGreen plasmid (Addgene, Catalog No. 39196). Lentivirus-containing supernatants were collected and concentrated by centrifugation (1,500 g for 1 hr at 4°C) ...
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bioRxiv - Cell Biology 2019Quote: Rab39b cDNA was cloned using Gibson Assembly into pEGF-N1-Flag plasmid (Addgene #60360 ...
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bioRxiv - Physiology 2019Quote: ... pLKO-puro FLAG SREBP1 was a gift from David Sabatini (Addgene plasmid # 32017). PPRE-X3-TK-luc was a gift from Bruce Spiegelman (Addgene plasmid # 1015) ...
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bioRxiv - Physiology 2019Quote: pAd-Track FLAG-SIRT1 was a gift from Pere Puigserver (Addgene plasmid # 8438). pAdtrack CMV plasmid was a gift from Bert Vogelstein (Addgene plasmid # 16405) ...
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bioRxiv - Biochemistry 2021Quote: ... pFRT/TO/FLAG/HA-DEST PABPC4 was from Thomas Tuschl (Addgene plasmid #19882) (Landthaler ...
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bioRxiv - Neuroscience 2021Quote: ... Flag-HA-HuD was obtained by cutting the pFRT-TODestFLAGHA_HuD plasmid (Addgene, #65757) with PaeI and BglII enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... HDAC6-FLAG (#13823) and pEF5B-FRT-GFP-aTAT1 (#27099) were purchased from Addgene. Tub-GFP-HDAC6 was made by PCR amplifying the HDAC6 sequence from the HDAC6-FLAG construct and ligating the PCR amplicon into the Tub-GFP construct ...
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bioRxiv - Cell Biology 2022Quote: The coding sequence of Mettl3 was amplified from pcDNA3/Flag-METTL3 (Addgene, #53739) and inserted into the lentiviral plasmid pLOXCMV (Qi et al ...
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bioRxiv - Physiology 2022Quote: ... FLAG-IKKβ-S177E/S181E (#11105) and HA-Ubiquitin (#18712) were acquired from Addgene. HA-TRAF6 was generated by cloning the TRAF6 fragment into pcDNA3-HA plasmid ...
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Histone H3K36me2-specific methyltransferase ASH1L is required for the MLL-AF9-induced leukemogenesisbioRxiv - Cancer Biology 2021Quote: The pMIG-FLAG-MLL-AF9 retroviral vectors as obtained from Addgene (Plasmid #71443). Retroviral vectors were generated by co-transfection of retroviral vectors with pGag-pol ...
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bioRxiv - Cancer Biology 2021Quote: ... The pcDNA5-FRT/TO-LacR-FLAG-TOPBP1 plasmid was obtained from Addgene (#31313). Point mutants were introduced by site-directed mutagenesis using Quikchange (Agilent) ...
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bioRxiv - Developmental Biology 2019Quote: ... pcDNA-HDAC6-FLAG was a gift from Dr.Tso-Pang Yao (Addgene plasmid # 30482).
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bioRxiv - Molecular Biology 2019Quote: ... Flag-PIAS4 WT plasmid was a gift from Ke Shuai (Addgene plasmid # 15208). RNF4-Myc-FLAG WT plasmid was purchased from OriGene (CAT# ...
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bioRxiv - Cell Biology 2019Quote: ... The pcDNA3-Flag-Apex-Nes was a gift from Alice Ting (Addgene, 49386). The N1-mTurquoise2 (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... A plasmid expressing anti-CRISPR protein (pEJS581; pCSDest2-AcrIIC4Hpa-FLAG-NLS; Addgene # 113436) was included in the triple-transfection packaging process to maintain intact rAAV:HDR:cleaved plasmids during production ...
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bioRxiv - Cell Biology 2021Quote: ... pcDNA-HDAC6-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30482) (Kawaguchi et al. ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected with plasmids expressing Flag-tagged mTOR (Plasmid #26603, Addgene) together with HA-tagged Raptor (Plasmid #8513 ...
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bioRxiv - Molecular Biology 2022Quote: ... K562 or HeLa cells were transfected with pEBB-Flag-GCN5 (Addgene, Plasmid #74784) or an empty vector using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: lenti SYN-FLAG-dCas9-KRAB-MeCP2 was a gift from Jeremy Day (Addgene plasmid # 155365 ...
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bioRxiv - Cancer Biology 2022Quote: ... pENTR4-FLAG (w210-2) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17423 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG-β-catenin K19R (Plasmid #Addgene plasmid # 44749; http://n2t.net/addgene:44749; RRID:Addgene_44749), FLAG- β-catenin K19R/K49R(Addgene plasmid #44751 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG- β-catenin K19R/K49R(Addgene plasmid #44751; http://n2t.net/addgene:44751; RRID:Addgene_44751)) ...
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bioRxiv - Cell Biology 2023Quote: ... and N131-N160 was inserted using the pEXPqcxip-hCCDC47-FLAG vector (Addgene, 159141) as a template and primers designed to include a C-terminus MYC-tag ...
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bioRxiv - Cancer Biology 2024Quote: ... C-terminally FLAG-tagged ASXL1 p.G646Wfs*12 and p.Y591X were obtained from Addgene. The MSCV-T2A-Puromycin vectors encoding 3xFLAG-tagged full-length ASXL1 and truncated ASXL1 were generated by PCR amplification followed by sub-cloning using Gibson assembly.
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bioRxiv - Immunology 2024Quote: ... pcDNA3-HA-RIPK3 (78804) and pcDNA3-FLAG-RIPK1 (78842) were procured from Addgene.
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... A viral vector containing a Cre expressing cassette (pAAV-CMV-HI-eGFP-Cre-WPRE-SV40, Addgene; #105545) was used to induce Fkbp5 deletion in Fkbp5lox/lox mice ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Biochemistry 2020Quote: hnRNPA2 LC (190-341), insoluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98657)
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bioRxiv - Biochemistry 2022Quote: AR-LBD (663-919) containing an N-terminal His-tag and encoded in pET15b plasmid (Addgene #89083) was expressed in Rosetta (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... 6 × 107 RPE1 cells were infected with the human sgRNA library (Human GeCKO v2 Library Cat#1000000048, Addgene) at an MOI of ∼0.3 ...
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bioRxiv - Cancer Biology 2021Quote: ... we cloned Myc-FLAG tagged POT1 into T7 expression vector (pcDNA 5/FRT, Addgene) and performed site-directed mutagenesis ...
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bioRxiv - Cell Biology 2022Quote: pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A was a gift from Nicolas Manel (Addgene plasmid # 86674 ...
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bioRxiv - Cell Biology 2019Quote: ... pFRT/FLAG/HA-DEST QKI was a gift from Thomas Tuschl (Addgene plasmid #19891)56 ...
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bioRxiv - Molecular Biology 2019Quote: ... RACK1-FLAG was cloned into pLenti CMV Puro DEST (w118-1) (Addgene plasmid #17452) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and pBa-EGFP-flag-BicD2594-FKBP (Addgene plasmid #63569; http://n2t.net/addgene:63569; RRID:Addgene_63569), pBa-FRB-3myc-KIF13A tail 361- 1749 (Addgene plasmid #64288 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-mNep2-FLAG (mNeptune2-N1, a gift from Michael Davidson (Addgene plasmid # 54837 ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids ordered were HDAC2 Flag (Addgene plasmid # 36829; http://n2t.net/addgene:36829; RRID:Addgene_36829), HDAC3 Flag (Addgene plasmid # 13819 ...
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bioRxiv - Cell Biology 2020Quote: ... or FLAG-MKL1 or S449A/T450A/S454A (Addgene plasmid 19845, Muehlich et al., 2008). Phenotypic analyses of siRNA treated cells were at 48-72 h post initial siRNA treatment ...
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bioRxiv - Neuroscience 2019Quote: ... astrocytes were transfected with 2 μg of pLYS1-FLAG-MitoGFP-HA (Addgene plasmid # 50057) which contains the pore-forming subunit of the mitochondrial calcium uniporter coupled to GFP or a mito-mCherry construct generated by subcloning the targeting sequence of the pLYS1-FLAG-MitoGFP-HA plasmid into the mcherry2-N1 vector (Addgene plasmid # 54517) ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... and sub-cloned into XbaI/EcoRI sites of Flag-HA-pcDNA3.1 vector (Addgene #52535). Fragments of Dnmt1 (2–290 ...
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bioRxiv - Cancer Biology 2021Quote: ... pRK5-TGFβRI-FLAG plasmid (#14833) and pCMV5B- TGFβRII plasmid (#11766) were purchased from Addgene.