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Citations for Addgene :
1 - 50 of 116 citations for ALLYL 11 12 EPOXY STEARATE TECH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... were transfected in DMEM with 2 % (v/v) FBS with 12 μg of pHR-CMV-TetO2 construct vector alongside 11 μg envelope plasmid (pMD2.G; Addgene #12259) and 11 μg packaging plasmid (psPAX2 ...
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bioRxiv - Cell Biology 2020Quote: ... Primers containing this targeting sequence (primers 11 and 12) were annealed and subcloned into the pU6-(BbsI) CBh-Cas9-T2A-mCherry vector (Addgene #64324) digested with BbsI.
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bioRxiv - Cell Biology 2020Quote: ... specifically: pETM-11 (AddGene) for Sar1 and pFASTBacHTb (AddGene ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas7-11 ORF was PCR amplified from pDF0229- DiCas7-11 (Addgene plasmid #172506) with Q5 High-Fidelity DNA polymerase (M0491 ...
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bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: ... 11 µg VSVG vector (Addgene #8454), and 16.5 µg of pPAX2 vector (Addgene #12260 ...
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bioRxiv - Cell Biology 2021Quote: ... and pSFFV_mNG2(11)1-10 (Addgene #82610), respectively ...
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bioRxiv - Biophysics 2021Quote: ... and 11 μg packaging plasmid (psPAX2; Addgene #12260) using PEI at 2.5:1 (w/w ...
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bioRxiv - Microbiology 2022Quote: ... and 11 μg of pMDLg/pRRE (Addgene # 12251) were co-transfected per tissue culture dish using Lipofectamine LTX reagent with PLUS reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Homology arms were cloned into pDD268 [11] (Addgene #132523) using NEBuilder Hifi DNA assembly Master Mix (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... 11 ug of transfer plasmid (dCas9-ZIM3-mCherry, Addgene #154473 or sgRNA libraries cloned into CROP-seq-opti ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmids as described previously.11 pLKO.1-Scrambled plasmids (Addgene, #136035) were used as negative control ...
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bioRxiv - Molecular Biology 2024Quote: ... pXPR_502 (11) was a gift from John Doench & David Root (Addgene plasmid # 96923 ...
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bioRxiv - Bioengineering 2021Quote: ... 12 µg pCMV (Addgene #12263), 3 µg pMD2.G (Addgene #12259 ...
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bioRxiv - Developmental Biology 2020Quote: ... The expression vector pCAG-mNG2(11) were derived from pCAG-ERT2CreERT2 (Addgene #13777) (Matsuda and Cepko ...
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bioRxiv - Bioengineering 2022Quote: ... 12 μg pCMV delta R8.2 (Addgene #12263), and 9 μg of the lentiCRISPR-v2 plasmid ...
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bioRxiv - Microbiology 2022Quote: ... Hyeran Choe [12] (Spike-Flag (Addgene #: 156420), Spike-Flag FKO (Addgene # ...
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bioRxiv - Molecular Biology 2021Quote: ... The pSFFV_mNG2(11)1-10 plasmid was a gift from Bo Huang (Addgene plasmid # 82610) (Feng et al. ...
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bioRxiv - Neuroscience 2022Quote: ... 250μl per site at an 11-degree angle) with AAV5-EF1α-DIO-ChR2-eYFP (Addgene) to selectively target neuronal populations expressing Cre ...
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bioRxiv - Molecular Biology 2023Quote: pDF0159 pCMV - huDisCas7-11 mammalian expression plasmid was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #172507 ...
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Progerin Can Induce DNA Damage in the Absence of Global Changes in Replication or Cell ProliferationbioRxiv - Molecular Biology 2024Quote: ... cell line pLB4/11 was stably transfected with plasmid pBABE-puro-GFP-progerin (Addgene plasmid # 17663) as described [45] ...
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bioRxiv - Neuroscience 2024Quote: ... the Lenti-X HEK293T was co-transfected with 11 μg of SAD B19-G (Addgene #32633). For every microgram of plasmid ...
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bioRxiv - Microbiology 2021Quote: ... and pBabe 12S E1A were purchased from Addgene. 82 selected VACV genes were amplified by PCR from the VACV WR genome and subcloned into pcDNA3.2-DEST using the Gateway cloning method (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... coli IYK-12 using pOSIP-CT (Addgene #45981) to create the strain IG-CR via clonetegration (54) ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-hsyn-eNpHR3.0-eY-FP (n=12; Addgene 26972 ...
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bioRxiv - Bioengineering 2024Quote: ... 12 μg of pCMV delta R8.2 (Addgene #12263) and 9 μg of the pooled gRNA vector library was added to 2 mL of Opti-MEM ...
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bioRxiv - Neuroscience 2020Quote: ... the experimental group (n = 12) received injections of AAV5-CaMKIIa-hM4Di-mCherry (titer 4.4×10^12 GC/ml; Addgene, MA, USA;) and the control group (n = 10 ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV.syn.jGCaMP8s.WPRE (AAV1, titer order of magnitude 10-12, Addgene) was injected ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... The inserts of the pENTR™11 constructs were transferred into the lentiviral expression vector pInducer20 (Addgene, 44012) using Gateway cloning (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Molecular Biology 2024Quote: ... pXPR_502 (11) was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923; RRID:Addgene_96923). pLX-sgRNA (29 ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2-retro-CAG-GFP (Addgene, 7×10^12 gc/ml), AAV2-retro-AAV-CAG-tdTomato (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... and pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV1, titer order of magnitude 10-12 Addgene) was injected 200 μm below the dura at 4 different sites (25 nL each ...
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bioRxiv - Neuroscience 2023Quote: ... 200nL of AAV9-CaMKII-Cre (10^12 gcp/mL Addgene) was injected at a depth of 300µm in each craniotomies ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli NiCo21 (DE3) competent cells were transformed with the plasmid encoding sumo-tag fused DiCas7-11 (Addgene: 172503). A single colony was picked and transferred to 100mL LB media containing 50 μg/mL ampicillin and grown for 12 hours at 37°C before inoculation into 1L LB culture ...
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bioRxiv - Neuroscience 2023Quote: ... Rats intended for fiber photometry experiments (n=11 females) received unilateral infusions of AAV9 FLEX-GCaMP6f (0.4uL, titer ∼1.4 × 1013 GC/mL, Addgene #100833) into the VP and retro-AAV Cre (0.4uL ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the U6-crRNA scaffold and the DiCas7-11 gene sequence from pDF0114 (Addgene #172508) and pDF0159 (Addgene #172507) ...
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bioRxiv - Genomics 2020Quote: THP-1 and MV4;11 cells were engineered to stably express humanized S.pyogenes Cas9 endonuclease by lentiviral transduction with the FUCas9Cherry vector (Addgene 70182) and subsequent FACS-selection for mCherry-positive cells (THP-1-Cas9 ...
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bioRxiv - Neuroscience 2021Quote: ... For the mScarlet the human H2B clustered histone 11 (H2BC11) ( accession #NM_021058) was fused in frame without a linker and cloned into the pAAV-CAG-tdTomato (Addgene #59462) using the sites KpnI and EcoRI at the 5 and 3 prime end respectively ...
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bioRxiv - Microbiology 2024Quote: ... wells were transfected with 500 ng of RSV Gag-GFP (11) and 125 ng of FLAG-tagged Med26 (a gift from Joan Conaway and Ronald Conaway [Addgene plasmid #15367 ...
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bioRxiv - Neuroscience 2023Quote: Th-cre rats were randomly assigned to a viral group and infused bilaterally with a cre-dependent AAV encoding either the inhibitory opsin archaerhodopsin T (ArchT; N = 11; 6 males; AAV5-CAG-FLEX-ArchT-tdTomato, Addgene) or a tdTomato fluorescent protein control (tdTomato ...
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bioRxiv - Bioengineering 2023Quote: ... Human codon optimized DisCas7-11 protein sequence and the mature DR guide scaffold with golden gate sites were PCR amplified from Addgene plasmids # 172507 and #172508 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5’-CGC-3’ to 5’-CAC-3’ at nucleotide positions 522-524) were produced by first combining 11 μg of lentivirus construct with 5.5 μg of pMD2.G (Addgene, #12259) and 8.25 μg of psPAX2 (Addgene ...
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bioRxiv - Genetics 2024Quote: ... 11 ug of transfer plasmid (dCas9-ZIM3-mCherry, Addgene #154473 or sgRNA libraries cloned into CROP-seq-opti, Addgene #106280), 7.5 μg of psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2022Quote: ... lentivirus was produced from the Brunello sgRNA library (12) (Addgene 73178) in 293FT cells (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2-retro-AAV-CAG-tdTomato (Addgene, 7×10^12 gc/ml), Cholera toxin subunit B CF-640 (Biotium ...
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bioRxiv - Synthetic Biology 2022Quote: ... we generated the complement InterTag-BFP by inserting the sequence encoding for SpyTag002-sfCherry11 –TagBFP amplified from pSFFV-SpyTag-sfCherry2(11)-TagBFP (Feng et al. [53] Addgene #117485) into the membrane expression vector pDisplay (Addgene #34842 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR-amplified sequences of mouse Prdm1 and Prdm14 enhancers 11 bearing terminal NotI restriction sites were cloned into PCR4-Shh::lacZ-H11 (Addgene, # 139098). Plasmid clones were validated by Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... The pSAD 11.G H2B:GFP was obtained by taking advantage of the pSAD 11.G F3 expression vector (kindly provided by M. Tripodi, Cambridge University, AddGene #32634). The nucleotide sequence encoding the histone H2B-tagged GFP was designed and ordered via GeneArts (ThermoFisher Scientific) ...