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Citations for Addgene :
551 - 600 of 2604 citations for 6H 5 Oxa 1 2a 4a triazacyclopenta cd pentalene 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Complementary gRNAs were annealed and subcloned into the pSpCas9(BB)-2A-GFP (pX-458) vector (#48138; Addgene) between BbsI endonuclease restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... and the pSpCas9(BB)-2A-Puro V2.0 (PX459) was a gift from Feng Zhang (Addgene plasmid #62988) (Ran et al ...
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bioRxiv - Neuroscience 2020Quote: ... 1:5 dilution in saline solution) or AAV1.CAG.DIO.tdTomato (control, 120 nl, 2.6×1013 vg/mL Addgene, diluted 1:5 in saline) was injected into AL ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dnmt3a CD and the C-terminal part of mouse Dnmt3L (3a3L) were amplified from pET28-Dnmt3a3L-sc27 (Addgene #71827). The resulting constructs (collectively ...
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bioRxiv - Molecular Biology 2023Quote: ... the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into pET-His6-MBP-TEV (Addgene #29656) by ligation-independent cloning following QB3 Macrolab protocols (https://qb3.berkeley.edu/facility/qb3-macrolab/) ...
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bioRxiv - Cancer Biology 2024Quote: ... The CDS’ were then used to replace the KRAB-dCas9-DHFR coding region in an inducible-expression lentiviral plasmid (RRID:Addgene_167935) by Gibson assembly (Vazyme ...
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bioRxiv - Molecular Biology 2019Quote: ... vector pSpCas9(BB)-2A-Puro (PX459) V2.0 (a gift from Feng Zhang, Addgene plasmid #62988; http://n2t.net/addgene:62988; RRID: Addgene_62988) was used ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). Two sgRNAs were used ...
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bioRxiv - Cell Biology 2020Quote: ... sgRNA was cloned into pSpCas9(BB)-2A-GFP (PX458) vector kindly provided by Feng Zhang (Addgene plasmid #48138) [28] ...
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bioRxiv - Cell Biology 2020Quote: ... and Smo−/− ESCs the following gRNAs sequences were cloned in the pX458_pSpCas9(BB)-2A-GFP vector (Addgene, #48138):
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bioRxiv - Immunology 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138http://n2t.net/addgene:48138;RRID:Addgene_48138).
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bioRxiv - Genetics 2021Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138 ; http://n2t.net/addgene:48138 ; RRID:Addgene_48138). Sequences of sgRNA are shown in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pCW57-GFP-2A-MCS plasmid was a gift from Adam Karpf (Addgene plasmid # 71783; http://n2t.net/addgene:71783; RRID:Addgene_71783). pGFP-P2A-CFTR-WT-T7 was mutagenized to delete the coding sequence in CFTR exon 23 ...
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bioRxiv - Developmental Biology 2021Quote: ... RD cells were transfected with the all-in-one Cas9/gRNA plasmid pSpCas9 BB-2A-GFP (PX458; Addgene; gRNA target sequence ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... sequences to target the MYC gene in the proximity of the BR-coding region were designed using the online software CRISPR Design Tool (http://crispr.mit.edu/) and cloned as DNA inserts into pSpCas9 (BB)-2A-GFP (PX458) (Addgene plasmid # 48138 ...
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bioRxiv - Neuroscience 2021Quote: Kir was amplified by PCR from pCAG-Myc-Kir2.1E224G/Y242F-2A-EGFP (similar to pCAG-Kir2.1-T2A-tdTomato, Addgene #60598 ...
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bioRxiv - Molecular Biology 2020Quote: ... was derived from E14 mouse ES cells and is homozygous for a Tir1-2A-Puro cassette (Addgene plasmid # 92142; http://n2t.net/addgene:92142; RRID:Addgene_92142) at the TIGRE locus.
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bioRxiv - Molecular Biology 2021Quote: pTRIP-SFFV-mtagBFP-2A STING and the parental empty vector were a gift from Nicolas Manel (respectively, Addgene plasmid # 102586 ...
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bioRxiv - Neuroscience 2019Quote: ... 5’-CTTCTAAAATGGGTGGCATA-3’ was designed using the CRISPR design tool (http://crispr.mit.edu) from the Zhang laboratory and cloned into plasmid pSpCas9(BB)-2A-Puro (Px459) V2.0 (Addgene, 62988). Plasmid constructs were purified with the Endo-free Plasmid Maxiprep kit (Qiagen) ...
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bioRxiv - Genetics 2019Quote: ... Cells were co-transfected with luciferase reporter plasmids (Figure 2A) or a positive control (pAP1-3, Addgene 71258) and a renilla normalisation control (pGL4.74 ...
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bioRxiv - Molecular Biology 2019Quote: ... upstream and downstream sgRNAs were cloned under an U6 promoter into the pSpCas9(BB)-2A-GFP (Addgene #48138) and the pSpCas9(BB)-2A-mCherry (generated in house ...
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). HeLa cells stably expressing C2-His6 or C2-Flag were generated by lentiviral transduction ...
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bioRxiv - Molecular Biology 2021Quote: ... The two sgRNAs targeting Dazl exon 6 were cloned into the pSpCas9(BB)-2A-GFP backbone (Addgene #48138). The knock-in led to the generation of a reporter cell line named Dazl-mScarlet-HygromycinR (DASH) ...
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bioRxiv - Cell Biology 2021Quote: ... candidate gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) plasmid vector (Addgene plasmid #48138; RRID: Addgene_48138) (Ran et al. ...
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bioRxiv - Cell Biology 2021Quote: ... candidate gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) plasmid vector (Addgene plasmid #48138; RRID: Addgene_48138) (Ran et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138) and was described previously49 ...
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bioRxiv - Neuroscience 2020Quote: ... pSpCas9n(BB)-2A-GFP (PX461) and pX330-U6-Chimeric_BB-CBh-hSpCas9 were a gift from Feng Zhang (Addgene plasmid # 48140 ...
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bioRxiv - Molecular Biology 2021Quote: ... EF1a-dCasRx-2A-EGFP was a gift from Patrick Hsu (Addgene plasmid # 109050; http://n2t.net/addgene:109050; RRID:Addgene_109050)) using oligonucleotides noted in Table S4 ...
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bioRxiv - Cancer Biology 2022Quote: ... human codon-optimized Streptococcus pyogenes wild-type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cat. No. 44719). Two chimeric guide RNA expression cassettes containing two of the following sgRNAs (sgRNA1 ...
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bioRxiv - Cancer Biology 2022Quote: ... the following sequences were cloned into pSpCas9(BB)- 2A-Puro (PX459) V2.0 (gift from Feng Zhang, Addgene #62988) targeting mouse Col6a1 ...
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bioRxiv - Cell Biology 2022Quote: ... The vector pSpCas9(BB)-2A-Puro (PX459) V2.0 was provided from Feng Zhang (plasmid 62988; Addgene, Cambridge MA) (Ran et al ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid # 48139 ; http://n2t.net/addgene:48139 ; RRID:Addgene_48139)50 The sgRNA oligos (sense ...
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bioRxiv - Systems Biology 2022Quote: pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). The oligonucleotides containing CRISPR guide RNA sequences (sgRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse and human sgRNAs were designed using the CHOPCHOP web tool: (http://chopchop.cbu.uib.no) and cloned into pSpCas9(BB)-2A-Puro (PX459) (Addgene #48139) and lentiCRISPRv2 (Addgene #52961).
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; http://n2t.net/addgene:48138; RRID:Addgene_48138).
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bioRxiv - Cancer Biology 2019Quote: ... was created using pSpCas9(BB)-2A-GFP (a gift from Dr. Feng Zhang, #48138, Addgene, http://n2t.net/addgene:48138 ; RRID:Addgene_48138) as a backbone vector [12] ...
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bioRxiv - Cancer Biology 2019Quote: ... and ligated into the pSpCas9(BB)-2A-EGFP vector (pX458; a gift from Feng Zhang; Addgene Plasmid #48138) at the BbsI cloning site ...
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bioRxiv - Developmental Biology 2019Quote: ... Annealed guides were then cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, plasmid 62988, PMID: 24157548). Ark -/- MEFs were transfected with 4 guide pairs in order to create the desired deletion using FUGENE (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and was inserted into BpiI sites of a pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene, Plasmid #62988). All sgRNAs used in the present study were designed using CRISPR DESIGN (http://crispr.mit.edu/ ...
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bioRxiv - Biochemistry 2019Quote: ... The sgRNA oligos were then cloned into the pSpCas9(BB)-2A-GFP (PX458) vector (a kind gift from Feng Zhang [Addgene plasmid # 48138; http://n2t.net/addgene:48138; RRID:Addgene_48138]). Jurkat T cells (clone E6-1 ...
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bioRxiv - Genetics 2020Quote: ... sgRNAs designed using the CRISPR Design Tool (http://crispr.mit.edu) were cloned into pSpCas9(BB)-2A-Puro (Addgene #48139) and transfected using Polyfect (Promega) ...
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bioRxiv - Physiology 2021Quote: ... was used for sgRNA-generation from the pSpCas9(BB)-2A-GFP (PX458) plasmid (Addgene, Watertown, Massachusetts, USA, #48138) from F ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells were transfected via PEI (https://www.addgene.org/protocols/transfection/) with pSpCas9(BB)-2A-GFP (PX458) - (Addgene #48138) carrying sgRNA for hSLC9A1 KO 5’-GTTTGCCAACTACGAACACG (SLC9A1:HGLibA_45399 ...
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bioRxiv - Cell Biology 2019Quote: ... phosphorylated oligonucleotides containing each target sequence into BbsI-digested pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988) 46 ...
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bioRxiv - Biochemistry 2020Quote: ... was designed by using http://crispr.mit.edu and cloned into the BbsI site of pSpCas9 (BB)–2A–Puro (PX459, Addgene). HeLa cells were transiently transfected with the PX459–sgRNF213-exon3 vector using FuGENE 6 Transfection Reagent (Promega) ...
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bioRxiv - Immunology 2020Quote: ... gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) plasmid (a gift from F. Zhang; Addgene, #48138) as described elsewhere [33] and Jurkat cells were transfected with either empty vector or the gRNA-encoding construct using the Nucleofector Solution 2M (5 mM KCl ...
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bioRxiv - Cell Biology 2021Quote: ... The pSpCas9 (BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid #48138; http://n2t.net/addgene:48138; RRID:Addgene_48138). The gRNA sequences used for CEP90 and MNR were 5′-GATGAGGAAATATCATCCGT-3′ and 5′-GTATAAAATACCCGACCACA-3′ respectively.
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bioRxiv - Cancer Biology 2022Quote: ... ADAR2-targeting gRNAs were cloned into the Cas9-expressing mammalian expression vector pSpCas9(BB)-2A-Puro PX459 (AddGene#158112 ...
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bioRxiv - Cancer Biology 2022Quote: Single guide RNA sequences targeting murine Lrp1 were obtained from the GeCKO v2 library (35) and cloned into the pSpCas(BB)-2A-Puro (PX459) V2.0 vector (RRID:Addgene_62988). B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: We replaced the ORF of the GFP marker in the pSpCas9(BB)2A-GFP plasmid (Addgene plasmid #48138) by the ORF of iRFP670 ...