Labshake search
Citations for Addgene :
101 - 150 of 259 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... They were then transiently co-transfected with the reporter plasmid pJM046 as well as the packaging plasmid psPAX2 (Addgene, #12260; http://n2t.net/addgene:12260; RRID:Addgene_12260) and the envelope plasmid pCAG-VSVG (Addgene ...
-
bioRxiv - Cancer Biology 2020Quote: ... as well as B16F10 and PANO2 cells overexpressing custom-made plasmid encoding KrasG12C(pKrasG12C; Addgene #64372; GFP-KrasG12C_2B_puro) were produced as described elsewhere (Agalioti et al. ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total plasmid DNA/well were used in appropriate combinations of the plasmids: pLVX-puro (Addgene), pLVX-puro-TRIM67-Flag ...
-
bioRxiv - Cell Biology 2023Quote: ... The test wells were transfected with pHBx and control was transfected with GFP expressing plasmid (Plasmid #27082, Addgene). After three days post transfection transfected cells were treated with low dose puromycin (0.5 μg/ml ...
-
bioRxiv - Plant Biology 2023Quote: ... as well as the B-module with mNeonGreen (amplified from an AddGene-derived template (Shaner et al., 2013)) and the C-module with LTI6b (amplified from total cellular A ...
-
bioRxiv - Genomics 2023Quote: ... All 3 of the 6 wells were transfected with 100 ng of pCAG-NLS-HA-Bxb1 (Addgene #51271) and 1,500 ng of donor attB library whereas the T25 was transfected with 1,000 ng of the Bxb1 containing plasmid and 5,000 ng attB library ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... pAAV5-flex-taCasp3-TEVp was a gift from Nirao Shah and Jim Wells (Addgene viral prep # 45580-AAV5; http://n2t.net/addgene:45580; RRID:Addgene_45580),59 pAAV9-EF1a-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (Addgene plasmid # 50460 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10cm plate) cells were transfected using PEI with psPAX2 (3.5µg, a gift from Didier Trono, Addgene #12260), pMD2.G (1.5µg ...
-
bioRxiv - Molecular Biology 2020Quote: ... NPCs were seeded onto laminin-coated plates and transduced by spinfection43 with CMV-rtTA (Addgene ID: 19780) and TetO-NGN2-P2A-Puro (Addgene 79049) ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were plated in 10-cm plates and transfected with plasmids expressing His6-SUMO1 (Addgene plasmid # 133770) or His6-SUMO2 (Addgene plasmid # 133771) ...
-
bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
-
bioRxiv - Biochemistry 2022Quote: ... The PRESTO-Tango plasmid kit was a gift from Bryan Roth (Addgene kit # 1000000068).
-
bioRxiv - Molecular Biology 2023Quote: The CRISPaint gene tagging kit was a gift from Veit Hornung (Addgene kit # 1000000086). To generate a targeting construct for AGO2 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... All GPCR plasmids originated from the Roth lab PRESTO-Tango kit (Addgene, Kit #1000000068) and were amplified in E ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The MoClo-YTK plasmid kit was a gift from John Dueber (Addgene kit # 1000000061). Enzymes used were from New England Biolabs unless stated otherwise ...
-
bioRxiv - Immunology 2019Quote: ... They were then transiently co-transfected with the reporter plasmid pJM046 as well as the packaging plasmid psPAX2 (Addgene, #12260 ...
-
bioRxiv - Molecular Biology 2020Quote: ... all the sgRNAs targeting genes of interest as well as controls were cloned into LRG2.1 (derived from U6-sgRNA-GFP, Addgene: 108098 - as described in ref (Tarumoto et al. ...
-
bioRxiv - Biochemistry 2020Quote: The MSP1E3D1 plasmid (with a His-tag as well as a TEV protease cleavage site, from Addgene, MA, USA) (26 ...
-
bioRxiv - Genetics 2021Quote: ... PT5/Cas9 cells were transfected with an equal-parts mixture of pLib6.4 containing an sgRNA library as well as pBS130 (26290, Addgene) using Effetene (301427 ...
-
bioRxiv - Bioengineering 2022Quote: ... To generate H2B-iRFP lentiviral particles HEK 293T cells were plated in a 6-well plate (7×105 cells per well) and transiently transfected with 1.5 μg of pLenti-pGK-DEST-H2B-iRFP vector (Addgene # 90237 ...
-
bioRxiv - Cancer Biology 2024Quote: ... containing the viral packaging system of second generation as well as the lentiviral construct of interest pCDH-EF1a-mCherry-EGFP-LC3B (RRID:Addgene_170446), respectively ...
-
bioRxiv - Plant Biology 2023Quote: ... and rbcS-E9 enhancers and their 169-bp 3′ and 5′ segments as well as the 35S enhancer were PCR amplified from pZS*11_4enh (Addgene no ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (1–2 x 105 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene; plasmid #136466 ...
-
bioRxiv - Cancer Biology 2024Quote: ... AAV-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene plasmid #45580; http://n2t.net/addgene:45580; RRID: Addgene_45580). The following day ...
-
bioRxiv - Neuroscience 2020Quote: ... Feng Zhang (Addgene kit #1000000019). Once assembled into a destination vector ...
-
bioRxiv - Cancer Biology 2022Quote: ... 90% confluent HEK29T3 cells in 100 mm plates were transfected with pMD2.G (1.5 μg; Addgene plasmid # 12259), psPAX2 (3.0 μg ...
-
bioRxiv - Bioengineering 2021Quote: ... The GoldenPiCS Kit was a gift from the Gasser/Mattanovich/Sauer group (Addgene kit #1000000133). All coding sequences were amplified with high-fidelity Phusion DNA Polymerase (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
-
bioRxiv - Developmental Biology 2023Quote: TALEN plasmids were constructed using the Platinum Gate TALEN Kit (Kit #1000000043, Addgene, Cambridge, MA) as previously described (Sakuma et al. ...
-
REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: ... The plasmid encoding the TurboID enzyme as well as Turbo-ID-NLS were obtained from Addgene (Branon et al., 2018). REEP4-TurboID-HA and REEP5-TurboID-HA constructs for the generation of inducible cell lines were generated by combining the sequences of REEP4 or REEP5 (from HA-REEP5 ...
-
bioRxiv - Cancer Biology 2022Quote: We cloned shRNAs against SCD and GPX4 as well as control shRNA in the Tet-pLKO-puro vector (Gift from Dmitri Wiederschain, Addgene plasmid #21915 ...
-
bioRxiv - Microbiology 2020Quote: ... as well as packaging vectors psPax2 and pMD2.G (kind gifts from from Didier Trono (Addgene plasmid #12259 and 12260).
-
bioRxiv - Developmental Biology 2019Quote: ... as well as pCS2TAL3-DD and pCS2TAL3-RR destination vectors (#37275 and #37276) (Dahlem et al., 2012) were obtained from Addgene. TAL effector domains and FokI nuclease were cloned into these destination vectors to form the final pCS2-TAL vector for each TALEN ...
-
bioRxiv - Molecular Biology 2022Quote: ... Virus was generated by plating 3.5×104 COS1 cells per well in a 6 well plate and transfecting them on the 2nd day with 1 ug total plasmid per well at a 5:3:2 ratio of lentiCRISPR:psPAX2(Addgene #12260):pMD2.G(Addgene#12259 ...
-
bioRxiv - Cell Biology 2023Quote: ... 2-6×105 cells in a 35 mM well were transfected with 0.4ug of DNA encoding a puro resistance plasmid (pCoPURO, Addgene #17533) using Effectene Transfection Reagent (Qiagen) ...
-
bioRxiv - Microbiology 2023Quote: A549-AT cells were seeded in a 96-well format and were transfected with 0.1 ng of pRL-SV40 (Addgene; #27163) together with either 0.1 μg M50 Super 8x TOPFlash (Addgene ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: SH-SY5Y cells were plated in 6-well plates at a density of 400,000 cells per well and transfected the following day with pEGFP-n1-APP (69924, Addgene plasmid) using the jetOPTIMUS transfection reagent (Polyplus ...
-
bioRxiv - Bioengineering 2023Quote: ... and a plasmid containing both an empty sgRNA scaffold as well as a gene sequence encoding SpCas9 (AddGene plasmid# 57819) with a final goal of LV positive control vector generation ...
-
bioRxiv - Genomics 2023Quote: The plasmids encoding PE2 (Plasmid#132775) as well as the plasmid for expressing the pegRNA (Plasmid #132777) were obtained from Addgene. PegRNAs were cloned into the plasmid backbone with paired oligonucleotides (IDT ...
-
bioRxiv - Microbiology 2023Quote: ... ANP32B or a non-targeting control gRNA as well as with the packaging plasmids pMD2.G and psPAX2 (gifts from Didier Trono, Addgene plasmids # 12259 and # 12260 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... along with left and right TALENs (pZT-C13-L1: Addgene #62196; pZT-C13-R1: Addgene #62197; 375 ng/well each) targeting the human Citrate Lyase Beta-Like (CLYBL ...
-
bioRxiv - Genomics 2024Quote: ... as well as the Rosa26- and Tigre-specific sgRNA-encoding plasmids (pX335-EN479, pX335-EN481 and pX330-EN1201 (Addgene #92144)) were all provided by E ...
-
bioRxiv - Cell Biology 2019Quote: ... U2OS and HEK293 cells were seeded into 10 cm plates 24 h prior to transfection with 2.5 µg of the I-SceI expression vector pCBA-SceI (Addgene plasmid # 26477 ...
-
bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
-
bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...
-
bioRxiv - Plant Biology 2020Quote: ... from Sylvestre Marillonnet (Addgene kit # 1000000044). Specific parts (target gRNA or siRNA sequence ...
-
bioRxiv - Synthetic Biology 2022Quote: The MoClo Toolkit (Addgene Kit #1000000044), MoClo Plant Parts Kit (Addgene Kit #1000000047) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
-
bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...