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• A common druggable signature of oncogenic CMYC, mutant KRAS and mutant p53 reveals functional redundancy and competition of the oncogenes in cancer

  Maria Grześ, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CMYC cds (Addgene #46970), KRAS4B G12V cds (Addgene #35633) ...
• Epigenetic remodeling and 3D chromatin reorganization governed by NKX2-1 drive neuroendocrine prostate cancer

  Xiaodong Lu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... NKX2-1 and mouse Foxa2 CDS were amplified using NKX2-1 (Addgene, #119173) and mFoxa2 (Addgene ...
• Intronization enhances expression of S-protein and other transgenes challenged by cryptic splicing

  Kärt Tomberg, et al., bioRxiv - Genetics 2021

  Quote: ... inserting the ALKBH5 CDS and mRuby3 CDS into the pLIX_403 vector (Addgene plasmid #41395). The linker region between ALKBH5 and mRuby3 was constructed manually by finding the consensus at each position for reads mapping to the end of the ALKBH5 CDS or the start of the mRuby3 CDS ...
• A common druggable signature of oncogenic CMYC, mutant KRAS and mutant p53 reveals functional redundancy and competition of the oncogenes in cancer

  Maria Grześ, et al., bioRxiv - Cancer Biology 2023

  Quote: ... KRAS4B G12V cds (Addgene #35633), p53 R175H or R273H cds (a gift from Dr Maciej Olszewski ...
• Heat shock induces alternative polyadenylation through dynamic DNA methylation-regulated chromatin looping

  Emily E. Fink, et al., bioRxiv - Cell Biology 2023

  Quote: ... and pcDNA3.1-MS2-Tet1-CD (Addgene) plasmids using Lipofectamine 2000 (Life Technologies ...
• The circadian clock regulates receptor-mediated immune responses to an herbivore-associated molecular pattern

  Natalia Guayazán Palacios, et al., bioRxiv - Plant Biology 2024

  Quote: ... luciferase CDS (pICSL80001, Addgene plasmid # 50326) and ocs terminator (pICH41432 ...
• Dot1L methyltransferase activity is a barrier to acquisition of pluripotency but not transdifferentiation

  Coral K. Wille, Rupa Sridharan, bioRxiv - Developmental Biology 2020

  Quote: ... The CDS of Cdh1 was amplified from Addgene 71366 and moved into the BstBI and NotI sites of pCDH-CMV-MCS-EF1α-Neo ...
• Characterizing a Lethal CAG-ACE2 Transgenic Mouse Model for SARS-CoV-2 infection with Using Cas9-Enhanced Nanopore Sequencing

  Alexander Smirnov, et al., bioRxiv - Genetics 2024

  Quote: ... was combined with the human ACE2 CDS (Addgene #1786) and the three polyadenylation signals (b-globin ...
• A conserved ubiquitin- and ESCRT-dependent pathway to regulate human lysosomal membrane proteins

  Weichao Zhang, et al., bioRxiv - Cell Biology 2020

  Quote: The CDS of Vps4A E228Q was purchased from Addgene (80351). Vps4A E228Q was cloned into the pCMV-HA (Clontech ...
• Molecular Contribution to Embryonic Aneuploidy and Genotypic Complexity During Initial Cleavage Divisions of Mammalian Development

  Kelsey E. Brooks, et al., bioRxiv - Developmental Biology 2020

  Quote: Plasmids containing the coding sequence (CDS) for mCitrine-Lifeact (Addgene #54733), which labels filamentous actin (F-actin) ...
• Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease

  Evelyn Tran, et al., bioRxiv - Cell Biology 2020

  Quote: ... hTERT CDS was subcloned from pBABE-puro-hTERT plasmid (Addgene 1771) into LeGO iT vector between BglII and EcoRI sites ...
• A neurogenetic mechanism of experience-dependent suppression of aggression

  Kenichi Ishii, et al., bioRxiv - Neuroscience 2020

  Quote: ... The nls::LexA::p65 CDS was amplified from pBPnlsLexA::p65Uw (RRID:Addgene_26230). The 1st exon of nvy ...
• Mitochondrial calcium signaling mediated transcriptional regulation of keratin filaments is a critical determinant of melanogenesis

  Jyoti Tanwar, et al., bioRxiv - Cell Biology 2023

  Quote: Plasmid containing CDS sequences of human MCU were taken from Addgene and restriction digestion was performed ...
• Heat shock induces alternative polyadenylation through dynamic DNA methylation-regulated chromatin looping

  Emily E. Fink, et al., bioRxiv - Cell Biology 2023

  Quote: ... the HCT116 cells were transfected with both pdCas9-Tet1-CD (Addgene) and pcDNA3.1-MS2-Tet1-CD (Addgene ...
• ARHGAP32 as a novel RhoGAP interacting with desmoplakin is required for desmosomal organization and assembly

  Hua Li, et al., bioRxiv - Cell Biology 2023

  Quote: ... ARHGAP32 and DSP CDS were inserted into pBiFC-VC155 (Addgene #22015) and pBiFCVN173 (Addgene #22010) ...
• Machine Learning and Directed Evolution of Base Editing Enzymes

  Ramiro M. Perrotta, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... The base Cas9 CDS was amplified from ABE8e plasmid (Addgene #138489) using the oligonucleotides BE_Npu_F and BE_pJC175e_R ...
• Targeted mutagenesis of specific genomic DNA sequences in animals for the in vivo generation of variant libraries

  Julia Falo-Sanjuan, et al., bioRxiv - Genetics 2024

  Quote: pattB-Actin5C-BE2(pActin5c-CD-dCas9m4-UDI-attB) was obtained from Addgene# 104879 (Marr and Potter ...
• Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease

  Evelyn Tran, et al., bioRxiv - Cell Biology 2020

  Quote: ... CDK4R24C coding sequence (CDS) was subcloned from pBABE-hygro-CDK4R24C plasmid (Addgene 11254) by PCR amplification into LeGO iG vector between BamHI and SbfI sites ...
• Leveraging single cell multiomic analyses to identify factors that drive human chondrocyte cell fate

  Divya Venkatasubramanian, et al., bioRxiv - Developmental Biology 2024

  Quote: ... NFATC2 CDS was transferred into the pInducer20 lentivirus destination vector (Addgene Plasmid #4401252) using Gateway LR Clonase to generate pInducer20-NFATC2.
• SATAY-Based Chemogenomic Screening uncovers Antifungal Resistance Mechanisms and Key Determinants of ATI-2307 and Chitosan Sensitivity

  Matthew T. Karadzas, et al., bioRxiv - Microbiology 2024

  Quote: ... The BdDRK1 CDS was then sub-cloned into p405-TEF1pr (Addgene plasmid #15968) using SpeI and PstI and T4 DNA ligase (Thermo Fisher Scientific #EL0011).
• Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway

  Sebastian Riedelbauch, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Each CDS was then cloned into two Gateway compatible bait (Addgene #111232 & #111236) and two prey (Addgene #111233 & #111237 ...
• Toxicity of extracellular cGAMP and its analogs to T cells is due to SLC7A1-mediated import

  Valentino Sudaryo, et al., bioRxiv - Immunology 2024

  Quote: A plasmid containing the CDS of human SLC7A2 (pDONR221_SLC7A2) was purchased from Addgene. A custom plasmid (pTwist-CMV ...
• Drosophila FMRP recruits the miRISC to target mRNAs to repress translation

  Navneeta Kaul, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553) and LD47780 (DGRC ...
• PRMT5-mediated histone methylation regulates alternative splicing via MECP2-PTBP1 to promote EMT in breast cancer hypoxia

  Srinivas Abhishek Mutnuru, et al., bioRxiv - Cancer Biology 2024

  Quote: ... the DNMT3A CD was digested out of the dCAS9-DNMT3A-puroV2 plasmid (Addgene, 74407) using BamHI and FseI ...
• Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease

  Evelyn Tran, et al., bioRxiv - Cell Biology 2020

  Quote: ... SV40 Large T antigen CDS was subcloned from pBABE-puro-SV40 LgT plasmid (Addgene 13970) into LeGO iG vector between BamHI and EcoRI sites ...
• A SUMO-dependent regulatory switch connects the piRNA pathway to the heterochromatin machinery in Drosophila

  Veselin I. Andreev, et al., bioRxiv - Molecular Biology 2021

  Quote: ... CDS fragments of interest were cloned into the entry Gal4 tethering vector (Addgene 128013-128014) and the resulting plasmids were electroporated into OSCs ...
• Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters

  Adam M. Zahm, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... pcDNA3.1_eGFP was generated by PCR amplifying the eGFP CDS from Arch(D95H)-eGFP (Addgene #51081) and inserting into pcDNA3.1(-)/myc-His A using the EcoRI and NotI restriction sites ...
• Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters

  Adam M. Zahm, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... pcDNA3.1-GPR91 was generated by PCR amplifying the GPR91 CDS from SUCNR1-Tango (Addgene #66507) (introducing a stop codon ...
• Prolonged over-expression of PLK4 amplifies centrosomes through formation of inter-connected centrosome rosette clusters

  Selahattin Can Özcan, et al., bioRxiv - Cell Biology 2023

  Quote: ... FLAG-PLK4 cDNA (CDS) was cloned into the lentiviral pCW57-hygro plasmid (Addgene plasmid, 80922) by double digestion with NheI and BamHI and ligation with T4 ligase ...
• Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters

  Adam M. Zahm, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... pcDNA3.1_Signal-Flag-DRD2 was generated by PCR amplifying the DRD2 CDS from DRD2-Tango (Addgene #66269) (introducing a stop codon ...
• Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters

  Adam M. Zahm, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... pcDNA3.1_Signal-Flag-HTR2A was generated by PCR amplifying the HTR2A CDS from HTR2A-Tango (Addgene #66409) (introducing a stop codon ...
• Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters

  Adam M. Zahm, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... pcDNA3.1_Signal-Flag-ADRB2 was generated by PCR amplifying the ADRB2 CDS from ADRB2-Tango (Addgene #66220) (introducing a stop codon ...
• Engineering Regionally-Activated Drugs for Neuroscience

  Zhimin Huang, et al., bioRxiv - Bioengineering 2023

  Quote: ... the protein coding sequence (CDS) of RLuc8.6 was amplified from pcDNA-RLuc8.6-53559 (Addgene ID 87125) and subcloned into the vector pRSETb115 (Addgene ID 89536 ...
• Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis

  Chang-Yi Chiu, et al., bioRxiv - Plant Biology 2024

  Quote: ... the CDS of AtCNIH5 was amplified by PCR and subcloned into UBQ10:sXVE: S10-(MCS) (Addgene plasmid # 108177 ...
• Hypoxia promotes airway differentiation in the human lung epithelium

  Ziqi Dong, et al., bioRxiv - Developmental Biology 2024

  Quote: ... the HIF1A and HIF2A CDS were cloned from plasmids gifted from William Kaelin (Addgene, #87261, #25956) and inserted into Tet-ON vectors with EF1a-TagRFP-2A-tet3G.58,60 For CRISPRi ...
• Acetylation-mediated phase control of the nucleolus regulates cellular acetyl-CoA responses

  Ryan Houston, et al., bioRxiv - Cell Biology 2020

  Quote: ... The NCL CDS was amplified from the GFP-Nucleolin plasmid (a gift form Michael Kastan, Addgene #28176)(Takagi et al. ...
• SARS-CoV-2 protein NSP2 enhances microRNA-mediated translational repression

  Parisa Naeli, et al., bioRxiv - Cell Biology 2023

  Quote: ... the AGO2 CDS was PCR-amplified using pFRT/FLAG/HA-DEST EIF2C2 plasmid (Addgene: Cat. # 19888[57]) as template and Q5 High-Fidelity DNA Polymerase (NEB ...
• Lactylation-driven FTO-mediated m⁶A modification of CDK2 aggravates diabetic microvascular anomalies

  Xue Chen, et al., bioRxiv - Cell Biology 2023

  Quote: CDS of zebrafish fto mRNA was synthesized and inserted into the pCS2+ plasmid (Addgene, Watertown, MA, USA) to get the linearized pCS2+-FTO plasmid for in vivo transcription ...
• DNA methylation shapes the Polycomb landscape during the exit from naïve pluripotency

  Julien Richard Albert, et al., bioRxiv - Molecular Biology 2023

  Quote: ... After an incubation of 1h at room temperature with a pA-Tn5 adapter complex (Addgene #124601), the Tn5 enzyme tagmentation reaction was performed by adding a buffer containing MgCl2 to the samples and incubating for 1h at 37°C ...
• The ventral CA2 region of the hippocampus and its differential contributions to social memory and social aggression

  Lara M. Boyle, et al., bioRxiv - Neuroscience 2024

  Quote: ... n=2) or AAV9.EF1a.dflox.hChR2(H134R).EYFP.WPRE.HGH (1×10^12 pp/mL, Addgene, cohort 2, n=2). Subject mice were additionally injected in vCA2 (AP −3.0 ...
• Cerebellar Activation Bidirectionally Regulates Nucleus Accumbens Core and Medial Shell

  Alexa F. D’Ambra, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1:2 dilution) or AAV5-pCAG-FLEX-EGFP-WPRE (1.1 x 1013 gc/ml, Addgene; 1:2 dilution) was injected in VTA (from bregma ...
• LRRC15 is an inhibitory receptor blocking SARS-CoV-2 spike-mediated entry in trans

  Jaewon Song, et al., bioRxiv - Microbiology 2021

  Quote: ... SARS-CoV-2 P.1 (Addgene, #170450), SARS-CoV-1 (Addgene ...
• A Cortical Site that Encodes Vocal Expression and Reception

  Thomas Pomberger, et al., bioRxiv - Neuroscience 2024

  Quote: ... AAV-2/1/CAG-Flex-EGFP (Addgene), pENN.AAV.hsyn.Cre.WPRE.hGH (AAV1 ...
• Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis

  Weidao Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: ... The Lnc956 and Trim28 coding regions (CDS) were inserted into pTOMO-IRES-EGFP lentiviral expression vector (Addgene, #26291). Lnc956 ORF1 ...
• An atypical NLR protein modulates the NRC immune receptor network

  Hiroaki Adachi, et al., bioRxiv - Plant Biology 2021

  Quote: ... The PCR product was cloned into pICSL01005 (Level 0 acceptor for CDS no stop modules, Addgene no.47996) as a level 0 module for Golden Gate assembly ...
• Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle

  Xiaodong Wang, et al., bioRxiv - Cell Biology 2024

  Quote: ... the full-length coding sequence (CDS) of CREB was amplified and inserted into the pcDNA3.1 plasmid (Addgene, USA). HEK293T cells were seeded in a 24-well plate and incubated for 24 hours ...
• Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis

  Chang-Yi Chiu, et al., bioRxiv - Plant Biology 2024

  Quote: ... the CDS of AtPHF1 and AtPHO1 were amplified by PCR and subcloned into UBQ10:sXVE: S10-(MCS) (Addgene plasmid # 108177 ...
• Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis

  Chang-Yi Chiu, et al., bioRxiv - Plant Biology 2024

  Quote: ... the CDS of AtPHT1;1 and AtPHO2 were amplified by PCR and subcloned into UBQ10:sXVE: (MCS)-S11 (Addgene plasmid # 108179 ...
• Visual intracortical and transthalamic pathways carry distinct information to cortical areas

  Antonin Blot, et al., bioRxiv - Neuroscience 2020

  Quote: ... AAV1.hSyn.GCaMP6f.WPRE.SV40 (120 nl, 2×1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 to 1:10 in saline) was injected either into V1 ...
• Engram cell connectivity as a mechanism for information encoding and memory function

  Clara Ortega-de San Luis, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV9-EF1a-DIO-hChR2-EYFP (1:2, Addgene viral prep # 20298- AAV9 ...