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Citations for Addgene :
401 - 450 of 2520 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pFRT/TO eGFP TIA1 (Addgene, catalog number: 106094) and pCMV3-GFP-hnRNPK (LSBio ...
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bioRxiv - Cell Biology 2023Quote: ... Cells transfected with EGFP-Actin (Addgene plasmid #31502) (52 ...
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bioRxiv - Biochemistry 2023Quote: ... GCK was cloned into pAG416GPD-EGFP-ccdB (Addgene plasmid 14316 ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... pCAG-Archon1-KGC-EGFP-ER2-WPRE (Addgene; #108423), using Gibson assembly (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... the piggyBac donor plasmid (PBCAG-eGFP, Addgene #40973) underwent several modifications ...
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bioRxiv - Neuroscience 2023Quote: ... Retrograde (AAVrg-hSyn-EGFP; AAVrg-hSyn-mCherry; Addgene) and anterograde (AAV1-hSyn-EGFP ...
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bioRxiv - Molecular Biology 2023Quote: The pHAGE-NFE2L2-IRES-EGFP plasmid (Addgene 116765) was used to produce lentiviral particles ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... PB-TRE-EGFP-EF1a-rtTA (Addgene, Plasmid #104454) was digested with NheI and KpnI and the Lmx1a CDS and IRES-GFP fragments were inserted using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Biophysics 2023Quote: ... Separate lentivectors containing EGFP-NPM1 (Addgene plasmid # 17578) and mRuby2-Lamin A/C (Addgene plasmid # 55901 ...
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bioRxiv - Neuroscience 2024Quote: ... we used pAAV-CW3SL-EGFP (Addgene plasmid #61463) as a backbone and replaced EGFP with PCR products that contained either mRuby3-2A-Cre-WPRE or mRuby3-2A-dCre-WPRE (the plasmids pAAV-Ef1a-fDIO-mRuby3-2A-Cre and pAAV-Ef1a-fDIO-mRuby3-2A-dCre respectively served as templates 28 ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV9-synP::DIO-EGFP (Addgene 100043-AAV9) were injected at 3×109 genome copies (0.25 µL ...
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bioRxiv - Molecular Biology 2024Quote: MSCs expressing pTRIP-SFFV-EGFP-NLS (Addgene #86677), pCDH-MiniNesprin1-GFP-cpstFRET (gifted by Maiuri laboratory) ...
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bioRxiv - Microbiology 2024Quote: ... in the lentiviral pLJM1-eGFP backbone (Addgene #19319).
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bioRxiv - Neuroscience 2024Quote: ... into the pAAV2-hSyn-eGFP viral vector (Addgene) by substituting the eGFP sequence ...
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bioRxiv - Cell Biology 2024Quote: ... Addgene clones were: eGFP-Rab7 WT (Addgene, #12605), eGFP-Rab7Q67L (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9-hSyn-Dio-eNpHR3.0-EGFP (Addgene, optogenetic inhibition), AAV9-hSyn-Dio-EGFP (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... or with Cas9-2A-EGFP (pX458; Addgene #48138) containing a gRNA against CSA ...
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bioRxiv - Neuroscience 2024Quote: ... AAV1-hSyn-Cre-EGFP (Addgene, optogenetics, photometry recording), Rabies virus (BrainVTA ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV5-hsyn-DIO-eGFP (Addgene, Catalog# 50457-AAV1), pAAV5-FLEX-tdTomato (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... pPBpuro-EGFP-eDHFR(69K6)-cRaf (Addgene no. 178849) was reported previously (Suzuki et al. ...
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bioRxiv - Biophysics 2024Quote: H2B-EGFP-N1 was ordered from Addgene (#11680). For EGFP-NLS construct ...
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bioRxiv - Cell Biology 2024Quote: ... Lenti-7TG (7xTcf-eGFP) plasmid (Addgene plasmid# 24314), pMD2.G (Addgene plasmid# 12259 ...
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bioRxiv - Bioengineering 2024Quote: ... and pCMV-T7-SpRY-P2A-EGFP (Addgene #139989) plasmids were used.
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3-EGFP was obtained from Addgene (Addgene #13031).
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bioRxiv - Cell Biology 2020Quote: ... using primer sequences 5’-TGTACCGGTCTCGAGGCCACCAT-GGTGGGTGAGG and 5’-AGATCCGGAGCTGTG-CCCCAGTTTGCTA, and cloned in place of EGFP in pT7-EGFP-C1-HsDCP1a (Tritschler et al., 2009) (Addgene # 25030). We then excised the FP635-DC-P1a cassette and blunt cloned into d2EGFPβ-glo-bin-UTR in place of the d2EGFP coding region.
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bioRxiv - Neuroscience 2021Quote: ... was mixed 1:1 with the retrogradely trafficked AAV encoding eGFP (AAVrg-hsyn-EGFP, 7.4 × 1012 vg/mL; Addgene, Watertown, MA) and infused into the BLA (AP ...
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bioRxiv - Developmental Biology 2021Quote: The ubi:hKCNH2 and ubi:eGFP cassettes were obtained by inserting either the full length coding sequence of hKCNH2 with the SV40 late polyadenylation signal (SV40pA) or eGFP from pME-eGFP (Tol2kit #383) downstream of the ubi promoter of pENTR5’_ubi (Addgene #27320). Plasmids (20 ng/μl ...
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified and cloned downstream of EGFP in the pAc5.1B-EGFP vector (a gift from Elisa Izaurralde; Addgene plasmid #21181) using the HindIII and EcoRI restriction sites to make pAcB5.1-EGFP-FMRP ...
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bioRxiv - Biophysics 2020Quote: The plasmid coding for human EGFR tagged with EGFP (hEGFR-EGFP) was a kind gift from Alexander Sorkin (Addgene plasmid #32751) (27) ...
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bioRxiv - Cell Biology 2020Quote: ... The tandem mitochondrial-targeted mCherry-eGFP mitophagy reporter plasmid (pCLBW-cox8-EGFP-mCherry) was a gift from David Chan (Addgene 78520). pLKO.1-shNT ...
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bioRxiv - Bioengineering 2022Quote: Lentiviral C→T base-editing reporter plasmid (LV-CS-sg1-GTG-EGFP-Pur) reporter cassette was constructed by amplifying the eGFP gene fragment from pLV-SI-112 (Addgene 131127) using the primer set AA-sg1-BC-CS-eGFP-FW/AA-eGFP-BamHI-RV followed by restriction enzyme digest by EcoRI/BamHI of the eGFP fragment and pLV-SI-112 ...
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bioRxiv - Cell Biology 2024Quote: ... The mammalian expression eGFP-MDM2 overexpression plasmid was generated by subcloning the eGFP-MDM2 insert into a pMK232 vector (Addgene #72834) following NdeI (NEB #R0111S)-XhoI (NEB #R0146 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: pAAV-EF1a-W56-Linker-PSD95.FingR-eGFP-CCR5TC and pAAV-EF1a-Scr-Linker-PSD95.FingR-eGFP-CCR5TC were generated by replacing the BamHI/CsiI sites of pAAV-EF1a-PSD95.FingR-eGFP-CCR5TC (Addgene #125691) with the PCR products of the gene fragments (Twist Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Rab7A and SNAP-Rab7A Q67L were made by cloning Rab7A sequence from EGFP-Rab7A and EGFP-Rab7A Q67L plasmids into pSNAP-tag (m) Vector (Addgene #101135). Soluble BFP (mTagBFP2 ...
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bioRxiv - Microbiology 2023Quote: ... pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with AAVS1-TRE3G-Mic10-FLAG-T2A-EGFP (Stephan et al., 2020, EMBOJ), AAVS1-TRE3G-DmMIC10-FLAG-T2A-EGFP, or AAVS1-TRE3G-EGFP (Qian et al., 2014) (Addgene 52343), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502).
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bioRxiv - Neuroscience 2023Quote: ... the cells were transiently transfected with green fluorescent protein (eGFP) (0.25 µg of cDNA/ml) and PIEZO1 channel plasmid (Addgene, #80925, 3 µg of cDNA/ml). For control experiments ...
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bioRxiv - Cell Biology 2020Quote: ... NuMA-N-PhusionRed-LOV2 and EGFP-Zdk1-NuMA-C were subcloned into a Moloney murine leukemia retroviral plasmid (gift from Iain Cheeseman; pIC291, Addgene #51403 (Welburn et al., 2009)) ...
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bioRxiv - Biochemistry 2020Quote: ... according to manufacturer’s instruction and then transferred to pLX302 lentiviral destination vector (addgene) or pDEST-CMV-N-EGFP vector (pDEST-CMV-N-EGFP was a gift from Robin Ketteler (Addgene plasmid # 122842) using recombination utilising the LR-Clonase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... an enhanced green fluorescent protein (eGFP) under the CaMKIIα promoter was used (AAV9-CaMKIIα-eGFP-WPRE; Addgene #50469, 2.4E+13 GC/mL). We infused the AAV in the dorsal hippocampus through the following coordinates relative to bregma ...
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bioRxiv - Microbiology 2020Quote: ... This vector expresses an endoplasmic reticulum (ER)-retained truncated Env-EGFP fusion protein. For linearization experiments (Fig. 5) we used plasmid pNL-EGFP/CMV/WPREdU3 (pNL-CMV-GFP) (Addgene, MA, USA). pNL4-3/Luc/Ori and pNL4-3/Luc/Kan were produced by molecular cloning into pNL4-3/Luc ...
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bioRxiv - Neuroscience 2021Quote: The CVS N2cdG-H2B-EGFP rabies virus was generated by replacing GFP in the rabies genomic plasmid RabV CVS-N2c(deltaG)-EGFP (Addgene, Plasmid #73461) with H2B-EGFP flanked by 5’ XmaI and 3’ NheI-KasI sites ...
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bioRxiv - Neuroscience 2021Quote: The SAD B19dG-H2B-EGFP virus was generated by inserting the H2B-EGFP sequence into the gG locus of the pSADdeltaG-F3 plasmid (Addgene, Plasmid #32634). The EnvA SAD B19dG-H2B-EGFP rabies viruses were generated from the genomic plasmid as described previously2 ...
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bioRxiv - Microbiology 2022Quote: ... The EGFP transgene in pLJM1-EGFP (a gift from was a gift from David Sabatini (Addgene plasmid # 19319, (Sancak et al., 2008) was replaced at the AgeI and EcoRI restriction sites with the following annealed oligos ...
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bioRxiv - Genetics 2023Quote: ... The puromycin resistance gene was then subsequently replaced with EGFP using an amplified fragment from the pMLS-SV40-EGFP plasmid 126 (Addgene plasmid # 46919). The expression and activity of the single-vector CROPseq plasmid was tested by cloning in a sgRNA targeting the DNMT3B (sgRNA sequence ...
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bioRxiv - Cell Biology 2023Quote: ... a donor plasmid pAAVS1-TRE3G-NGN2 was generated by replacing the EGFP sequence with N-terminal flag-tagged human NGN2 cDNA sequence in plasmid pAAVS1-TRE3G-EGFP (Addgene plasmid # 52343). 5μg of pAAVS1-TRE3G-NGN2 ...
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bioRxiv - Immunology 2021Quote: ... pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid #19319 ...
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bioRxiv - Cell Biology 2022Quote: ... or TetO-hNGN2-P2A-eGFP-T2A-PuroR (Addgene, 79823)) together with FUdeltaGW-rtTA (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: The RxCas13d-2A-eGFP expression plasmid (pXR001; Addgene #109049) and the corresponding guide RNA expression vector (pXR003 ...