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Citations for Addgene :
401 - 450 of 1340 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was transformed with pAC-BETAipi (Addgene) and pCDFDuet-OsD27 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was transformed with pAC-BETAipi (Addgene) and pCDFDuet-OsD27-trAtCCD7 to generate E ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S2060 cells were prepared containing pJC175e (Addgene #79219), a plasmid expressing pIII under control of the phage shock promoter32 ...
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bioRxiv - Biophysics 2020Quote: ... coli is pJCSUP35NM (1-253) (Addgene Plasmid number #1089). The over-expression of Sup35NM was induced using 1 M IPTG ...
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bioRxiv - Bioengineering 2019Quote: ... coli cells using the plasmid pRK793 (Addgene, Plasmid # 8827)55 ...
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bioRxiv - Genomics 2021Quote: ... coli containing pTXB1-3xFlag-pA-Tn5-FL (Addgene, #124601), a kind gift from (Kaya-Okur et al. ...
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bioRxiv - Cell Biology 2019Quote: We generated Schizosaccharomyces pombe strains with SNAP-tag (Addgene plasmid 87024) or mEGFP (Addgene plasmid 87023 ...
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bioRxiv - Biochemistry 2023Quote: ... or N-terminal His10-MBP Tag (2CT-10; Addgene Plasmid #37237) were chosen as the MBP tag has been shown to increase the solubility of select proteins [9] ...
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bioRxiv - Plant Biology 2023Quote: ... a C-terminal 3×FLAG® epitope tag (pICSL50007; Addgene #50308), and 3′ UTR and terminator sequences from Agrobacterium tumefaciens nopaline synthase (AtuNOST ...
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bioRxiv - Cell Biology 2022Quote: ... The dual-tag Galectin3 plasmid was acquired from Addgene (ref. 64149). The TBC1D15 coding sequence was subcloned into vectors pEGFPC1 and pGEX6P1 for expression in mammalian and bacterial cells ...
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bioRxiv - Cancer Biology 2022Quote: The pLentiCRISPR E plasmid (Addgene, 78852, a gift from Phillip Abbosh) was modified with different selectable marker genes ...
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bioRxiv - Cancer Biology 2024Quote: ... The lentiviral E-cadherin-EGFP were generated from pCS-CG (Addgene) for E-cadherin knock-in of MDA-MB-231 as previously described [58] ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Immunology 2022Quote: ... pVitro-hygro-N-myc-hVps34/Vps15-C-V5-his-plasmid (Addgene #24055), pEGFP-Atg14L (Addgene #21635 ...
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bioRxiv - Biochemistry 2022Quote: pQE-60 roGFP2-His was a gift from Tobias Dick (Addgene #65046)(50) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid for expression of EPS15-GFP-His was obtained from Addgene (#170860). EPS15D177S was generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... and the SLC35A1 gene were designed using CRISPR direct (https://crispr.dbcls.jp/; S1 Table) and each sequence was cloned into plentiCRISPR plasmids [37] (Addgene plasmid #52961 ...
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bioRxiv - Cancer Biology 2023Quote: ... using pLenti_mENPP1_fwd and pLenti_mENPP1_rev primers in Table S1 and inserted into the XbaI-BamHI sites of pLenti-CMV-GFP-Puro (Addgene). To clone pLenti-CMV-mENPP1-T238A-GFP-Puro plasmid ...
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bioRxiv - Plant Biology 2021Quote: ... coli strains containing pAC-BETA-At (Addgene plasmid no. #53288), pAC-ZETA (Addgene plasmid no ...
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bioRxiv - Bioengineering 2019Quote: ... and bioluminescent ilux pGEX(-) Escherichia coli was purchased from Addgene, a gift from Dr ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-CodonPlus (DE3) cells containing pET28a-MSP1D1delltaH5 vector (Addgene cat# 71714 was kindly purchased by Dr ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DHL708 (a gift from Johan Paulsson, Addgene plasmid # 98417) [8] for all insertions ...
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bioRxiv - Biochemistry 2023Quote: ... coli with lambda phosphatase spectinomycin-resistant plasmid (Addgene plasmid #79748). We used either wild-type ...
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bioRxiv - Biophysics 2023Quote: ... coli biotin ligase BirA from the T7 promoter (Addgene#109424) in E ...
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bioRxiv - Neuroscience 2021Quote: ... CalR-HaloTag-Snap-tag-KDEL (ER-localizing) or GCaMP6f (Addgene 100837-AAV1). On day 14-18 the neurons were incubated for at least 2 h with 1 μM of the corresponding MaPCa dyes containing 0.04% Pluronic-F127 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Biophysics 2022Quote: ... The His6-SUMO tag was subsequently cleaved with human SenP1 (Addgene #16356) 27 and separated on Ni2+-Histrap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: GST-tag human HP1⍺ was a kind gift from Naoko Tanese (Addgene plasmid # 24074 ...
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bioRxiv - Genomics 2023Quote: ... Mito-tag plasmids were based on pMXs-3XHA-EGFP-OMP25 (Addgene, #83356) and pMXs-3XMyc-EGFP-OMP25 (Addgene ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-SCoV-2S (Spike envelope plasmid; Sinobiologicals – Cat #: VG40589-UT) and pLJM1-EGFP (reporter; Addgene plasmid #19319) in a ratio of 1:1:2 respectively ...
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bioRxiv - Genetics 2020Quote: ... we co-transfected pcDNA3.1-SARS2-Spike or pcDNA3.1-SARS2-SpikeD614G (see above) with pcDNA3.1-ACE2 (Addgene 1786) at a 1:1 ratio (Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032);34 this gene was cloned into a modified pcDNA 3.1 backbone (Clontech-Takara ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032).
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bioRxiv - Developmental Biology 2020Quote: ... Bot oligo – 5’ aaacGGGTGAGACCCATGTATTTc3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Cancer Biology 2023Quote: ... and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella)67 for expressing the mutant Cyclin E ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
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bioRxiv - Cell Biology 2022Quote: ... guide RNAs targeting a gene of interest (Table S1) were cloned into pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene plasmid #50946), using the BbsI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs (Table S1) were designed using an online CRISPR design tool (crispr.mit.edu) and cloned into a gRNA cloning vector (Addgene, 41824) following option B from the gRNA Synthesis Protocol(53) ...
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bioRxiv - Neuroscience 2022Quote: ... we used stereotactically targeted virus injections of rAAV S1 FLEX-CAG-jGCaMP7s-WPRE (Lot v28549, Addgene, #104495 AAV-1) into the MSDB of 3— 6 months old ChAT-Cre mice ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding oligonucleotides (Table S1) were annealed and cloned in the pU6-BbsI-chiRNA vector linearized with BbsI (Addgene plasmid #45946 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression vector used for the pseudovirion SARS CoV2-19 was from Addgene (pCDNA 3.1_Spike_Del19 #155297). The packaging vectors and Lenti ZsGreen were from Addgene (pHIV-Zsgreen #18121 ...
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bioRxiv - Immunology 2021Quote: ... pTWist-WIV1-CoV (a gift from Alejandro Balazs (Addgene plasmid # 164438; http://n2t.net/addgene:164438; RRID:Addgene_164438), or pHDM-MERS-CoV Spike and a luciferase/GFP reporter (pHAGE-CMV-Luc2-IRES542 ZsGreen-W ...
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bioRxiv - Immunology 2020Quote: ... The insert was ordered from GeneArt and subcloned into pcDNA3.1 expression plasmid using unique NotI-EcoRI sites to create pcDNA3.1-SpyTag-RBD (GenBank and Addgene deposition in progress) (see Figure S1) ...