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Citations for Addgene :
401 - 450 of 1446 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Male PV-Cre and both male and female Ai6 mice were secured in a stereotaxic frame and bilateral injections of 200-300 nl AAV1 virus vectors (AAV1-EF1a-DIO-ChETA-eYFP (Addgene: 26968) to PV-Cre mice and pENN-AAV1-hSyn-Cre-WPRE-hGH (Addgene ...
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bioRxiv - Cell Biology 2023Quote: A synthetic sequence corresponding to 2A peptide from Thoseaasigna virus capsid protein and BspTI (AflII) restriction site was first cloned to pCXLE-EGFP (Plasmid #27082, Addgene, www.addgene.org) (Okita et al. ...
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bioRxiv - Neuroscience 2023Quote: ... We expressed the soma-targeted opsin ST-ChrimsonR 43 in excitatory neurons by injecting a virus (1012 titer; AAV2/2 camKII-KV2.1-ChrimsonR-FusionRed; Addgene, plasmid #102771) into the craniotomy ...
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bioRxiv - Neuroscience 2023Quote: ... into the AC and a retrograde Cre virus: pENN/AAVrg-hSyn-Cre-WPRE-hGH (1.8E+13 vg/ml, 200nl, Addgene catalog #105553) into the pStr ...
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bioRxiv - Neuroscience 2023Quote: ... We bilaterally injected Cre-dependent DREADD virus (Roth, 2016): AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4E+13 vg/ml, 350nl, Addgene catalog # 44362) or AAV5-hSyn-DIO-mCherry (2.6E+13 vg/ml ...
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bioRxiv - Neuroscience 2024Quote: A bicistronic virus expressing both opsin and GCaMP indicator, AAV9-hSyn-DIO-jGCaMP8s-P2A-stChrimsonR(LaFosse et al., 2023) (Addgene, 174007) was diluted in phosphate-buffered saline (final titer ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced using standard virus production methods by co-transfecting target and packaging plasmids (psPAX2 – Addgene12260 and pMD2.G-Addgene 12259) into HEK293T cells ...
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bioRxiv - Neuroscience 2024Quote: ... and xylazine (10mg/kg) or Isoflurane (5.0%) and given unilateral infusions of a retrograde transducing adeno-associated virus (AAVretro-CAG- GFP Addgene #37825; [32]) into the vlPAG (for males ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transduced at an MOI < 0.3 with virus made via the pJR103 dual guide RNA plasmid (a kind gift from Marco Jost & Jonathan Weissman, Addgene #187242) (33) ...
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bioRxiv - Neuroscience 2024Quote: ... a small volume of AAV8-hSyn-DIO-hM3D(Gq)-mCherry virus (Addgene 44361, final titer 2 x 1012 pp per mL) was injected bilaterally into RSC (AP ...
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bioRxiv - Microbiology 2020Quote: ... and the matrix protein from vesicular stomatitis virus was amplified from pVSV eGFP dG (a gift from Connie Cepko; Addgene plasmid #31842) as described above using the primers listed in Table S2 ...
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bioRxiv - Microbiology 2021Quote: The Tobacco mosaic virus (TMV) expression vector pJL-TRBO has been previously described [57] and was a gift from John Lindbo (Addgene plasmid # 80082). The TMV vector containing p26:GFP has also been previously described [41] ...
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bioRxiv - Genomics 2022Quote: ... BPTF BD with an N-terminal 6xHistidine (6His) tag and Tobacco Etch Virus (TEV) Protease cleavage site was from Addgene (plasmid 39111). This was modified using the Q5 Site-directed mutagenesis kit (New England Biolabs [NEB] ...
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bioRxiv - Neuroscience 2022Quote: ... into the OB and the retrograde virus AAVrg-CamKIIα-mCherry (80 µl at titer ≥ 7×10¹² vg/mL, #114469-AAVrg, Addgene, MA, USA) into the HP ...
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bioRxiv - Neuroscience 2021Quote: ... the adeno-associated virus AAV2/1-Syn-FLEX-mRuby2-CSG-P2A-GCaMP6m-WPRE-SV40 (titer: 2.9 x 1013 GC per ml, Addgene accession no. 102816) in combination with AAV2/1.CamKII0.4.Cre.SV40 (titer ...
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bioRxiv - Pathology 2020Quote: ... Lentiviral vector pLV-mCherry and vesicular stomatitis virus glycoprotein (VSV-G) expression vector pMD2.G were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 S gene (GenBank ...
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bioRxiv - Neuroscience 2021Quote: The CVS N2cdG-H2B-EGFP rabies virus was generated by replacing GFP in the rabies genomic plasmid RabV CVS-N2c(deltaG)-EGFP (Addgene, Plasmid #73461) with H2B-EGFP flanked by 5’ XmaI and 3’ NheI-KasI sites ...
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bioRxiv - Neuroscience 2021Quote: The SAD B19dG-H2B-EGFP virus was generated by inserting the H2B-EGFP sequence into the gG locus of the pSADdeltaG-F3 plasmid (Addgene, Plasmid #32634). The EnvA SAD B19dG-H2B-EGFP rabies viruses were generated from the genomic plasmid as described previously2 ...
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bioRxiv - Neuroscience 2020Quote: ... Non-Cre-dependent expression of hM4D(Gi) was mediated by administration of an AAV8-CaMKIIa-hM4D(Gi)-mCherry virus (Addgene; plasmid #50477) while that of a GFP reporter by administration of an AAV5-CaMKII-GFP virus (Boyden ...
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bioRxiv - Immunology 2022Quote: ... the cells were transduced with virus produced using the lentiCas9-EGFP plasmid (a gift from Phil Sharp & Feng Zhang, Addgene plasmid # 63592), single-cell sorted using a Sony SH800 cell sorter ...
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bioRxiv - Cell Biology 2022Quote: Rac1 activation was achieved by expressing PA-Rac1 via retro-virus infection using pBabe-TetCMV-puro-mCherry-PA-Rac1 (Addgene Plasmid #22035) in MV3 cells ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2) or a GFAP-GFP Adeno-associated virus (AAV) construct (Addgene plasmid #50473) for use in animal experiments (utilized in Figure 3 and Figure 4) ...
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bioRxiv - Immunology 2022Quote: ... Virus was generated by transfecting the sgRNA plasmid constructs into HEK 293T cells with the psPAX2 packaging (Didier Trono, Addgene plasmid #12260) and pCMV-VSVG envelope (Bob Weinberg ...
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bioRxiv - Neuroscience 2023Quote: ... For DREADD experiments 6-7 week old mice were injected bilaterally with the inhibitory virus AAV-hSyn-hM4D(Gi)-mCherry (AddGene, 50475-AAV2) or control virus AAV-hSyn-EYFP (AddGene ...
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bioRxiv - Neuroscience 2023Quote: ... or left IC (0.9 mm caudal and 1 mm lateral from lambda suture) to inject 100-200 nL of pAAV-Syn-Chronos-GFP virus (Addgene #59170-AAV1). At the end of the surgery ...
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bioRxiv - Cancer Biology 2023Quote: ... or pLenti-PGK-tdTomato-Akaluc (TdT-AkaLuc) virus (subcloned from pLenti-PGK-Venus-Akaluc(neo)) and HR180-LGR5-iCT plasmids through Gibson assembly (Addgene 124701; 129094). For cecal injections ...
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bioRxiv - Neuroscience 2023Quote: ... Two small holes were made in the skull above the injection sites and 200nl (100nl/min) of AAV8-hSyn-DIO-mCherry or AAV8- hSyn-DIO-hm4Di-mCherry virus (Addgene, Watertown, MA) was infused in both hemispheres of the PVN using a 1µL Hamilton syringe (0° angle ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector 1B (N-terminal 6x His (His6) tag followed by Tobacco etch virus (TEV) protease cleavage site) (Addgene plasmid: 29653).
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bioRxiv - Neuroscience 2024Quote: ... We infected 4 DIV neurons prepared from PV-Cre or SST-Cre mice with adeno-associated virus (AAV) expressing Cre-dependent GCaMP7s (Addgene# 104495-AAV1) - pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE was a gift from Douglas Kim & GENIE Project (Addgene plasmid #104495 ...
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bioRxiv - Neuroscience 2024Quote: ... Gi DREADD virus (n=25,13 males, 12 females: AAV8-hSyn-DIO-hM4Di-mCherry,≥ 1×101 3 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express inhibitory designer receptors in VP GABA neurons ...
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bioRxiv - Neuroscience 2024Quote: Cre-dependent recombinant adeno-associated virus (rAAV) for GCaMP7f (rAAV1-syn-FLEX-jGCaMP7f-WPRE, Addgene #1944820-AAV1, titer: ≥1:1013 vg/mL) were used to express GCaMP7f in the hippocampus of Vgat-Cre mice ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2-Ef1a-DIO-hChR2(H134R)-mCherry (University of North Carolina viral core) or a control virus AAV1-hSyn-DIO-mCherry (Addgene #50459-AAV1) was injected unilaterally in the DMS (250 nl ...
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bioRxiv - Biochemistry 2024Quote: ... was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3 ...
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bioRxiv - Neuroscience 2022Quote: ... 400nl of a Cre-expressing virus that is retrogradely transported back one synapse (AAV-pmSyn1-EBFP-Cre, titer 1.00 x 1013, Addgene 51507-AAVrg, lot 27021) was injected into external segment of globus pallidus (GPe ...
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bioRxiv - Neuroscience 2021Quote: Pyramidal cell imaging experiments were performed by injecting a recombinant adeno-associated virus (rAAV) encoding GCaMP6f (rAAV1-Syn-GCaMP6f-WPRE-SV40, Addgene/Penn Vector Core) into male wild-type mice.
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bioRxiv - Neuroscience 2020Quote: One adult mouse (~ 2 months) was stereotaxically injected with a GCaMP6f construct (AAV5.CamKII.GCaMP6f.WPRE.SV40 virus, Addgene # 100834; 0.4 μL at 0.06 μl/min) in hippocampal CA1 ...
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bioRxiv - Neuroscience 2020Quote: ... A small (~0.5 mm) craniotomy was made and the virus AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6s.WPRSE.SV40 (Addgene 50942, titer 1.9 × 1013 vg/ml, −50 nL) was injected into the hippocampus (from bregma ...
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bioRxiv - Neuroscience 2022Quote: ... one group of animals (n=6) received a bilateral injection of a virus driving expression of the calcium indicator GCaMP7s (AAV9-hSyn-GCaMP7s-WPRE; Addgene; 300nL per side) targeting the CA1v at the following stereotaxic coordinates ...
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bioRxiv - Neuroscience 2024Quote: ... DREADD+ group) was bilaterally injected in the dDG with pAAV5-CaMKIIa-hM4D(Gi)-mCherry (virus titer ≥ 3×1012 vg/ml, Addgene, North Carolina, USA). Inhibitory DREADDs are activated by the artificial ligand clozapine-N-oxide (CNO ...
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bioRxiv - Neuroscience 2022Quote: ... ACS-4500) using Lipofectamine 2000 in FBS free Optimem together with the virus packaging plasmids (psPAX2 and pMD2-G, Addgene # 12260 and # 12259) and the plasmid expressing either wild-type U4atac or an Empty Vector ...
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bioRxiv - Developmental Biology 2023Quote: ... a total of 9.45 μg of each library plasmids combined with 6.75 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmids 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Genetics 2023Quote: ... a total of 13.6 μg core enhancer perturbation library plasmids with 5.44 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmid 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Neuroscience 2022Quote: ... mice were first injected with a helper virus (AAV5- hSyn-FLEX-splitTVA-2A-mCherry-2A-B19G; produced in-house, modified after Addgene plasmid no. 52473) into MEC ...
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bioRxiv - Neuroscience 2024Quote: ... 0.6-0.8 uL of a 1:1 mixture of GAD1-cre and mCherry virus (AAV8-hSyn-DIO-mCherry, ≥ 1×101 3 vg/mL; Addgene, Watertown, MA, USA) was injected bilaterally into VP ...
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bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
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bioRxiv - Developmental Biology 2024Quote: ... a total of 9.45 μg of the library plasmid combined with 6.75 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmids 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Neuroscience 2024Quote: ... For fiber photometry experiments Fig 4 and 4S: dual virus strategy of AAVretro-pEF1a-DIO-FLPo-WPRE-hGHpA (Addgene; 87306-AAVrg; 7×10e12) in PPN and AAVDJ-hEF1a-dFRT-jGCaMP7s(rev)-dFRT-WPRE-hGHp(A ...
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bioRxiv - Molecular Biology 2024Quote: ... the former was first deleted with a cassette containing the hygromycin B resistance marker (hphRMX) fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid #73898; RRID:Addgene_73898)64 ...
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bioRxiv - Genetics 2024Quote: ... and either a virus coding for the Cre-dependent expression of an excitatory DREADD receptor (AAV8-hSyn-DIO-hM3Dq-mCherry; experimental group) or a control virus (AAV8-hSyn-DIO-mCherry; control group; Addgene catalog # 114472-AAV8) was injected into the LC (Fig ...
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bioRxiv - Genetics 2024Quote: ... and a virus coding for the Cre-dependent expression of an excitatory DREADD receptor (AAV8-hSyn-DIO-hM3Dq-mCherry; Addgene catalog # 44361-AAV8) was injected into the LC (Fig ...