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Citations for Addgene :
401 - 450 of 727 citations for Ebola Virus Envelope Glycoprotein GP1 Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... or shRNA constructs in combination with the psPAX2 packaging and pCMV-VSV-G envelope plasmids (Addgene plasmid #12260 and #8454) using FuGene HD (Promega ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were transfected with the pLenti-VIM plasmid in combinations with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene) using TransIT_293 transfection reagent (Mirus) ...
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bioRxiv - Microbiology 2020Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Cell Biology 2022Quote: ... tat (transcriptional regulator) and rev (post-transcriptional regulator) and the envelope plasmid psPAX2 encoding VSV-G coat protein were purchased from Addgene repository ...
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bioRxiv - Immunology 2022Quote: ... or the scrambled shRNA sequence: CCGGCA-ACAAGATGAAGAGCACCAATTTTT were transfected overnight into 293 LentiX packaging cells (Takoro) along with VSV-G plasmid envelope (Addgene), aiming to generate shLRBA and shControl encoding lentiviruses ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259 ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253 ...
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bioRxiv - Biochemistry 2023Quote: ... 8 µg psPAX2 packaging plasmid DNA and 1 µg pMD2.G envelope plasmid DNA (both gifts from Didier Trono, Addgene plasmid no ...
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bioRxiv - Cancer Biology 2023Quote: ... These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr, addgene#8455) and envelope plasmid (pCMV-VSV-G, Addgene#8454) were used for lipofectamine 2000 mediated transfection of 293FT cells to produce lentiviral pseudo typed particles ...
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bioRxiv - Cell Biology 2023Quote: ... at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G: Addgene 12259 ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant DNA constructs cloned in lentiviral plasmids described above were co-transfected with packaging and envelope plasmids psPAX2 (gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Microbiology 2023Quote: ... semi-confluent HEK 293T cells in 10cm cell culture grade petri dishes were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Molecular Biology 2024Quote: ... ORFs of interest were overexpressed using second-generation lentiviral system that consisted of pCMV-VSV-G (Weinberg lab) envelope plasmid and PsPAX2 (Trono lab) packaging plasmid acquired from Addgene. HEK293t cells were transfected with plasmid DNA at a ratio of 5:4:1 transfer plasmid:envelope plasmid:packaging plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by transfecting HEK 293T cells with 12 μg of pLJM1-mEGFP or pLJM1ALKBH5 plasmids with 6 μg of psPAX2 packaging plasmid and 2 μg of pMD2.G envelope plasmid (Addgene). The medium was replaced 16h after transfection ...
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bioRxiv - Genomics 2024Quote: ... Lentivirus was produced in HEK293T cells co-expressing the shRNA plasmid together with psPAX2 packaging plasmid and pVSV-G envelope plasmid (Addgene). Virus was concentrated using Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2024Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Cell Biology 2023Quote: ... to co- transfect HEK 293FT cells with plasmids containing the packaging gag/pol and envelope pCMV- 435VSV-G genes and either the pMRX-IB-HaloTag7-mGFP-KDEL (Addgene, 184904 ...
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bioRxiv - Neuroscience 2022Quote: ... or control virus pAAV-CaMKIIa-EGFP (Addgene Cat# 50469-AAV5, titer: 4.3x1012) into the CA2 using the following coordinates from Bregma ...
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bioRxiv - Neuroscience 2021Quote: ... The control virus (AAV8-hSyn-DIO-mCherry, 2.3×1013 GC/mL, Addgene) was injected according to the same procedure ...
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bioRxiv - Biochemistry 2021Quote: Tobacco Etch Virus protease (TEV) was a gift from Helena Berglund (Addgene plasmid # 125194 ...
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bioRxiv - Neuroscience 2022Quote: ... a virus expressing GCaMP6s (AAV9.Syn.GCaMP6s.WPRE.SV40, ~1.9 × 1013 GC/ml; Addgene # 100843) was injected unilaterally in the A13 while for DREADD experiments viruses for reversibly activating (AVV8-Syn-hM3D-Gq-mCherry ...
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bioRxiv - Neuroscience 2019Quote: ... or AAV5-hDlx-GqDREADD-dTomato (3.15×10^15 virus molecules/mL) (Addgene plasmid # 83897 ...
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bioRxiv - Neuroscience 2019Quote: ... Virus (1012 titer; AAV2/2 camKII-KV2.1-ChrimsonR-FusionRed; Addgene, plasmid #102771) was injected 400 μm below the dura (4-10 sites ...
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bioRxiv - Neuroscience 2020Quote: ... 1.2 ul of AAV8-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene, USA) were bilaterally injected into 6 PV-Cre animals bilaterally ±0.5 mm lateral to midline ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5μl pGP-AAVrg-syn-jGCaMP7f-WPRE virus 25 (1.85× 1013GC/ml; Addgene) was infused into the NAc (A/P ...
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bioRxiv - Neuroscience 2022Quote: ... the virus used was an AAV5-FLOX-ChR2-mCherry (Addgene, #20297-AAV5) or a AAV5-Ef1a-DIO-eYFP (UNC Vector Core) ...
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bioRxiv - Immunology 2023Quote: ... Fresh virus of (MSCV-myc-CAR-2A-Thy1.1, Anjana Rao, Addgene: 127890) containing chimeric antigen receptor (CAR ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µl of AAV9.CAG.GCaMP6s.WPRE.SV40 or AAV9.syn.GCaMP8s-WPRE virus (Addgene, USA) was injected subcutaneously in the nape of the neck ...
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bioRxiv - Genetics 2023Quote: Library plasmid pools were packaged into lenti-virus by PsPAX2 (Addgene 12260) and pMD2.G (Addgene 12259 ...
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bioRxiv - Neuroscience 2023Quote: ... Virus (AAV1-S5E2-jGCaMP6f; Addgene #135632-AAV1; diluted 1:3 in saline) was injected into the RSC (AP ...
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bioRxiv - Neuroscience 2023Quote: ... a 1 μL dot of AAV9/hSyn/GCaMP6f virus (Addgene, Watertown, MA) diluted 1-5x from commercial titer (2.8x1013 to ∼1x1012 units/mL ...
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bioRxiv - Neuroscience 2023Quote: ... an AAV retrograde virus expressing pAAV-hSyn-hM4D(Gi)-mCherry (Addgene #50475) or pAAV-hSyn-hM3D(Gq)-mCherry (Addgene #50474 ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus package plasmids psPAX2 and pMD2.G were purchased from Addgene. To produce the lentivirus ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-CAG-hChR2-tdTomato (virus made in the host institute, using Addgene plasmid #28017 a gift from Karel Svoboda) ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1-hSyn-eNpHR3.0-EYFP (virus made in the host institute, using Addgene plasmid #26972 ...
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bioRxiv - Neuroscience 2023Quote: ... stGtACR2-expressingadeno-associated virus(AAV) vector (pAAV_hSyn1-SIO-stGtACR2-FusionRed, Addgene, #105677) stock solution was diluted to 5 × 1011 gc/ml titers ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were transduced with the AAV-CamKII-GFP virus (Addgene 50469-AAV9), and neurons with GFP-positive signals were selected for patch clamp experiments.
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bioRxiv - Neuroscience 2023Quote: ... AAV1-Syn-flex-GCaMP6f (virus made in the host institute, using Addgene plasmid #100833 ...
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bioRxiv - Developmental Biology 2023Quote: Commercially available sequences for tobacco etch virus protease (TEVp, Addgene Plasmid #8835), tobacco vein mottling virus protease (TVMVp ...
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bioRxiv - Neuroscience 2024Quote: Adeno-associated virus (AAV) type 2 carrying cre-dependent ChR2-eYFP (Addgene plasmid 20298 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hepatitis Delta Virus sequence from the pSVL(D3) plasmid99 (Addgene plasmid #29335) (https://www.addgene.org/29335/) ...
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bioRxiv - Neuroscience 2024Quote: ... Virus pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (Addgene viral prep #1120050-AAV5) was injected into layer 2/3 of the primary somatosensory barrel (S1BF ...
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bioRxiv - Biophysics 2021Quote: ... were transfected in DMEM with 2 % (v/v) FBS with 12 μg of pHR-CMV-TetO2 construct vector alongside 11 μg envelope plasmid (pMD2.G; Addgene #12259) and 11 μg packaging plasmid (psPAX2 ...
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bioRxiv - Cancer Biology 2020Quote: The dCas9-KRAB constructs were transfected in 293T cells with the 2nd generation lentiviral packaging and envelope plasmids psPAX2 and pMD2.g (Addgene, USA), using CaCl2 2.5M (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... transfections were performed using the the pLenti-CMV-DEST vectors together with the lentivirus envelope and package plasmids pMD2.G (Addgene, #12259) and psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2019Quote: ... gRNA was cloned into a mU6 expression cassette and then moved into an AAV backbone expressing a nuclear envelope-embedded (KASH-tagged) eGFP reporter (Addgene 131683) by PCR amplification and ligation-independent cloning (AAV-GRID1) ...
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bioRxiv - Cell Biology 2023Quote: ... The following day, cells were co-transfected (using Lipofectamine 3000 treatment, as described above) with pMD2.g (envelope plasmid, Addgene #12259), psPAX2 (packaging plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... The vector V2 CRISPR DNA Plasmid (1ug) was co-transfected in 293T cells along with 3 µg of the viral envelope PMD2 (Addgene # 12259) and 4 µg of the viral packing PsPAX (Addgene #12260 ...
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bioRxiv - Cancer Biology 2024Quote: High-titre lentiviral supernatants were produced by transient transfection of 293T/17 cells with second-generation packaging/envelope vectors pRRE (Addgene #12251), pREV (Addgene #115989) ...
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bioRxiv - Neuroscience 2021Quote: ... High titer (>1012GC/ml) AAV2/1-hSyn-Cre-WPREhGH virus (Addgene 105553-AAV1) was diluted 1:8 or 1:4 in 0.9% saline and 1µl was injected under the forepaw skin ...