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Citations for Addgene :
401 - 450 of 2252 citations for Dengue Virus Serotype 3 Envelope Protein Mouse Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: HEK293 cells were transfected with PPRE-H2b-eGFP (59) (Addgene #84393) using Invitrogen Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... of the parental cells (HEK293) with lentiCRISPR v2 plasmid57 (Addgene #52961) containing target specific gRNAs listed in Methods Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tag and MBP-tag were attached to its N- and C-terminus respectively (Addgene ID 169195). The cleavage at G//A resulted in 25 and 42 kDa products ...
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bioRxiv - Molecular Biology 2021Quote: ... The mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8xHis tag was obtained from Addgene (#145145). The mammalian expression vector for soluble ACE2 pcDNA3-sACE2 (WT)-sfGFP (#145171 ...
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bioRxiv - Cell Biology 2022Quote: ... The mammalian expression plasmid for Ism1 with C-terminal myc-6xhis tag plasmid for recombinant Ism1 protein production was from Addgene (#173046).
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bioRxiv - Genomics 2019Quote: Lentiviral vectors were produced by transfection of HEK 293T cells with the envelope (psPAX2, Addgene #12260), packaging (pMD2.G ...
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bioRxiv - Cancer Biology 2020Quote: ... the gag/pol plasmid pCMV-dr8.91 and the VSV-g envelope plasmid pMD2.G (Addgene, 12259). The plasmids were diluted in H2O and 2.5 M CaCl2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus preparation for stable cell line generation was done with pMD2.G envelope plasmid (Addgene #12259) and psPAX2 packaging plasmid (Addgene 12260 ...
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bioRxiv - Genomics 2020Quote: ... and the lentiviral envelope vector pMD2.G (kind gift from the Didier Trono lab, Addgene #12259) were co-transfected with the respective lentiviral expression vector (∼1:1:3 molar ratio ...
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bioRxiv - Molecular Biology 2022Quote: is a lentivirus envelope expression vector that was a gift from Didier Trono (Addgene plasmid # 12259). The plasmid expresses a viral envelope protein and the vesicular stomatitis virus G glycoprotein (VSV-G).
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bioRxiv - Neuroscience 2020Quote: One adult mouse (~ 2 months) was stereotaxically injected with a GCaMP6f construct (AAV5.CamKII.GCaMP6f.WPRE.SV40 virus, Addgene # 100834; 0.4 μL at 0.06 μl/min) in hippocampal CA1 ...
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bioRxiv - Neuroscience 2022Quote: The Cre-dependent viral vector pAAV-Ef1a-DIO -hChR2(E123T/T159C)-EYFP - serotype AAV9 - (ChR2) was a gift from Karl Deisseroth (Addgene plasmid # 35509 ...
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bioRxiv - Neuroscience 2020Quote: ... was mediated by a co-administration of a trans-synaptic CAV2-Cre virus (Plateforme de Vectorologie de Montpellier (PVM)) and a pAAV8-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene; plasmid #44362) while that of the mChrerry reporter alone was mediated by co-administration of the CAV2-Cre virus and a pAAV8-hSyn-DIO-mCherry virus (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... while that of the mChrerry reporter alone was mediated by co-administration of the CAV2-Cre virus and a pAAV8-hSyn-DIO-mCherry virus (Addgene; plasmid #50459) called ‘Empty’ throughout ...
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bioRxiv - Neuroscience 2023Quote: ... and mice were injected with either the AAV5-DIO-hTyr experimental virus or a comparable AAV5-DIO-EYFP control virus (Addgene, plasmid #27056). Due to the volume of virus required for these experiments ...
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bioRxiv - Immunology 2019Quote: ... and 6-His tag (Addgene plasmid# 50803) [36] ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139112)
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139116)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139117)
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bioRxiv - Biophysics 2022Quote: ... media was refreshed with standard growth media and cells were co-transfected with SNAP-mGluR2 (no FLAG-tag) and chimeric G protein (Gqo5, Addgene plasmid #24500) (1:2 w/w ...
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bioRxiv - Developmental Biology 2022Quote: The pTT3-eGFP vector was constructed by replacing the C-terminal tag encoding region of a bait protein vector (52) (Addgene ID 36150) with eGFP ...
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bioRxiv - Cell Biology 2023Quote: ... polyspora Hop1319-529 into UC Berkeley Macrolab vectors to generate N-terminal TEV protease-cleavable His6- or His6-maltose binding protein tags (Addgene #29666, 29706). DNA binding mutants of S ...
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bioRxiv - Biochemistry 2024Quote: The plasmid encoding amino acids 1-393 of the p53 protein with a FLAG tag at the C-terminus (Addgene plasmid #10838) was used as a template for site-directed mutagenesis to delete the N-terminal regions spanning amino acids 1-31 ...
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bioRxiv - Neuroscience 2020Quote: ... AAV virus was packaged with pAd-deltaF6 (Addgene #112867) and the helper pAAV2/5 (Addgene #104964) ...
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bioRxiv - Neuroscience 2021Quote: ... All virus was obtained from Addgene (Watertown, MA, USA). Injections were performed at 5 sites ...
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bioRxiv - Neuroscience 2021Quote: A barcoded Sindbis virus library (JK100L2, Addgene plasmid #79785) was generated and used for MAPseq and BARseq experiments as described previously34–37 ...
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bioRxiv - Developmental Biology 2020Quote: ... pMDLg/pRRE and pRSV-Rev helper virus vectors (Addgene). Culture medium was collected and ultracentrifuged to concentrate viral particles ...
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bioRxiv - Neuroscience 2021Quote: ... Cre-dependent AAV5-Syn-FLEX-ChrimsonR-tdTomato virus (Addgene) was injected in PFC (1.8 AP ...
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bioRxiv - Physiology 2023Quote: ... We unilaterally injected 0.6 µl of virus (pAAV.Syn.Flex.GCaMP6f.WPRE.SV40, Addgene viral prep #100833-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... or vector virus AAV8-hSyn-DIO-mCherry (Addgene 50459) at day −28 ...
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bioRxiv - Neuroscience 2022Quote: ... a virus solution (rAAV1-hSyn-jGCamP7f, 104488-AAV1, Addgene) was injected under stereotaxic conditions (400 nL ...
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bioRxiv - Neuroscience 2022Quote: ... AAV-hSynapsin1-FLEx-axon-GCaMP6s virus (Addgene, MA, USA) was injected bilaterally ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... adeno-associated virus encoding AAV8-hSyn-DIO-hM3Dq.mcherry (Addgene #44361 ...
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bioRxiv - Neuroscience 2023Quote: ... except that a retrograde-cre virus (pENN.AAV.hSyn.Cre.WPRE.hGH; Addgene #105553) or sterile PBS was injected bilaterally in the IC of 6-8 week old GtACR1 fl/fl mice (Jackson stock # 033089 ...
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bioRxiv - Developmental Biology 2023Quote: ... tobacco vein mottling virus protease (TVMVp; Addgene Plasmid #8832), and human rhinovirus 3C protease (HRV 3Cp ...
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bioRxiv - Neuroscience 2024Quote: ... 250 nl of AAVrg-CAG-mCherry virus (Addgene #59462) were intracranially injected unilaterally at 50 nl/min in the anterior cingulate cortex (ACC ...
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bioRxiv - Immunology 2019Quote: ... GXMR-CAR containing the lentiviral vectors pMD2.G (VSV-G envelope-expressing plasmid; Addgene; cat. no. 12259) and psPAX2 (second-generation lentiviral packaging plasmid ...
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bioRxiv - Biophysics 2020Quote: ... psPAX2 packaging plasmid (plasmid #12260) and pMD2.G envelope plasmid (plasmid #12259) were from Addgene (Watertown, MA). N-terminally Cy5-labeled peptides were synthesized and purified to > 95% purity by Gen-Script (Piscataway ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-SCoV-2S (Spike envelope plasmid; Sinobiologicals – Cat #: VG40589-UT) and pLJM1-EGFP (reporter; Addgene plasmid #19319) in a ratio of 1:1:2 respectively ...
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bioRxiv - Biochemistry 2020Quote: ... the envelope plasmid pMD2.G (both were gifts from Dr Didier Trono; Addgene plasmids 12260 and 12259) and pLX302 (a gift from Dr David Root ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260) and pMD2.G (Addgene 12259) ...
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bioRxiv - Immunology 2021Quote: ... 750 ng psPAX2 packaging plasmid and 250 ng pMD2.G envelope plasmid (Addgene plasmids #12260 and #12259). On the following day the virus-containing medium was collected and filtered using 0.45 µm filter (Millipore Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The envelope pCMV-VSV-G plasmid (from Bob Weinberg (Addgene #8454; http://n2t.net/addgene:8454; RRID: Addgene_8454)) and psPAX2 plasmid (from Didier Trono (Addgene #12260) ...
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bioRxiv - Microbiology 2023Quote: ... and 2.25 μg of the envelope plasmid pMD2.G (a gift from Didier Trono; Addgene plasmid #12259), all diluted in OptiMEM (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... were added to each well along with 1 μl of Syn1-EBFP-Cre (serotype, 2-1; titer, 6×1012 genomes/mL; MOI, ∼8-0.08; Addgene, 51507-AAV1) delivered at full strength or diluted 1:103-104 ...
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bioRxiv - Neuroscience 2019Quote: ... Expression of soma-targeted-ChrimsonR was achieved by stereotaxic injection of AAV serotype 1 carrying ChrimsonR-EYFP fused to a Kv2.1 somatic lo alization motif (“fle - ChrimsonR-EYFP-kv”, Addgene plasmid #135319). Injections were made into right visual cortex of mice aged between P26-P53 ...
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bioRxiv - Cell Biology 2020Quote: mCherry-ER fusion protein was amplified by PCR from mCherry-ER-3 plasmid (Addgene) and cloned into Gateway modified pBABE (in house) ...
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bioRxiv - Neuroscience 2019Quote: ... with different fluorescent protein genes and helper plasmids (3 μg pcDNA-SADB19N (Addgene, 32630), 1.5 μg pcDNA-SADB19P (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SUMO-SpyTag003 (N-terminal His6 tag-SUMO protein-SpyTag003) was cloned previously by Irsyad Khairil Anuar (University of Oxford) (GenBank and Addgene deposition in progress). pDEST14-SpyCatcher003-TEVs-SpyTag003DA (‘Masked SpyCatcher0003’ ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... followed by a GSG linker and mouse IgG2a Fc fusion (amino acid 99-330; UniProtKB/Swiss-Prot P01863) were synthesized and then cloned into pENTR (Addgene Plasmid #17398) using InFusion cloning according to manufacturer’s instructions (Clontech) ...