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Citations for Addgene :
351 - 400 of 477 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... SKO cells were transfected with pSH-EFIRES-P-GFP(1-10)opti AAVS1 Safe Harbor integration plasmid (Addgene plasmid # 129416 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells grown in twelve 10 cm dishes were co-transfected with active myc-mTOR E2914K mutant (Addgene) and HA-Raptor (Kim et al. ...
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bioRxiv - Microbiology 2020Quote: ... pQCXIP-BSR-GFP11 and pQCXIP-GFP1-10 were from Yutaka Hata 51 (Addgene plasmid #68716; http://n2t.net/addgene:68716; RRID:Addgene_68716 and Addgene plasmid #68715 ...
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bioRxiv - Immunology 2020Quote: mApple-Dectin1A-C-10 was a gift from Michael Davidson (Addgene plasmid # 54883; http://n2t.net/addgene:54883; RRID:Addgene_54883). pEGFP-DC-SIGN was a generous gift from Ken Jacobson[12] ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells at 70% confluence were transfected with 10 μg of pMRX-IP-GFP-LC3-RFP (#84573, Addgene), 5 μg of VSV.G (#14888 ...
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bioRxiv - Cell Biology 2021Quote: ... pSJ1256 (pFA6a-link-yGFP1-10-CaURA3MX) was a gift from Sue Jaspersen (Addgene plasmid # 86419; http://n2t.net/addgene:86419; RRID:Addgene_86419).
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bioRxiv - Cell Biology 2020Quote: ... the cDNA of human CAV1 was PCR amplified from the mammalian expression plasmid Emerald-CAV1 C-10 (Addgene No ...
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bioRxiv - Cell Biology 2022Quote: ... These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0, 50 mM NaCl, 1 mM EDTA) as recommended by Addgene (https://www.addgene.org/protocols/annealed-oligo-cloning) ...
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bioRxiv - Microbiology 2022Quote: ... mEmerald-MAP4-C-10 (a gift from Dr. Michael Davidson (Addgene plasmid # 54152; http://n2t.net/addgene:54152; RRID:Addgene_54152)) ...
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bioRxiv - Neuroscience 2024Quote: ... or pAAV-hSyn-EGFP a gift from Bryan Roth (titer ≥ 7×10¹² genome copies per ml; Addgene viral prep # 50465-AAV1; http://n2t.net/addgene:50465; RRID:Addgene_50465) were injected into the dorsolateral (100-200 nl ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in a sterile 0.9% NaCl solution/plasmid mix containing 10 μg of pT3-MYC (Addgene #92046), 10 μg of pX330-p53 (Addgene 59910) ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry-LaminB1-10 plasmid was a gift from Michael Davidson (Addgene plasmid #55069; http://n2t.net/addgene:55069; RRID:Addgene_55069).
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bioRxiv - Neuroscience 2023Quote: ... or an empty vector (control, AAV5-Ef1a-DIO EYFP at titer ≥ 1×10¹³ vg/mL, Addgene plasmid # 27056) were utilized ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid for STIM1-mApple was prepared in our lab as described previously123 and that for mEmerald-TOMM20-N-10 was a gift from Michael Davidson (http://n2t.net/addgene:54282 ; RRID:Addgene_54282).
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bioRxiv - Neuroscience 2024Quote: The pAAV-hSyn-DIO-hM4D(Gi)-mCherry (DREADD virus) (4*10^12 gc/ml, Addgene, United States, addgene.org) or pAAV-hSyn-DIO-mCherry (4.2*10^12 gc/ml ...
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Integration of distinct cortical inputs to primary and higher order inhibitory cells of the thalamusbioRxiv - Neuroscience 2024Quote: ... AAV1-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (titer = 2.8 to 9.9×10^12 vg/ml, Addgene).
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bioRxiv - Neuroscience 2024Quote: ... transgenic mice received injection of AAV2rg-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA (0.2 μL; titer = 8.0 x 10^12 gc/mL; Addgene, #37120-AAVrg) into the RSC and were terminally sacrificed after four weeks.
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bioRxiv - Neuroscience 2024Quote: ... and AAV1-CAG-FLEX-rc[Jaws-KGC-GFP-ER2] (titer: 2.5 × 10¹³ GC/ml; Addgene catalog #: 84445-AAV1) were used in the experiments ...
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bioRxiv - Neuroscience 2024Quote: ... DV: -0.3) and two adjacent injections of 0.2μl retroAAV-Ef1a-Cre (55636-AAVrg, Addgene, 7×10¹² vg/mL) were injected at a rate of 0.05μl/min ...
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bioRxiv - Neuroscience 2024Quote: ... or the control reporter mCherry (AAV5-hsyn-DIO-mcherry, Addgene #50459 titre: 1:4 – 7×10¹² GC/mL).
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bioRxiv - Synthetic Biology 2021Quote: ... The pAR-Ec611 (ArEc-Rev1-611) plasmid harboring medium-error-rate polymerase TP-DNAP1_611 (≥10−6 s.p.b.) was obtained from Addgene (Watertown, MA).
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bioRxiv - Microbiology 2020Quote: ... 10 μg pQXCIH-TMPRRS2-HA was co-transfected with polyethylenimine (PEI) with 6.5 μg pBS-gag-pol (Addgene #35614) and 5 μg pMD2.G in a 10 cm dish of 70% confluent HEK-293T cells in Opti-MEM I (1X ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids encoding the Spike protein were a mixture of (10 µg) of pcDNA3.1-SARS2-Spike (Addgene plasmid 145032) and (3 µg ...
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bioRxiv - Cancer Biology 2021Quote: ... The 293 cells were seeded in 10-cm-diameter dishes and transfected with pCMV-dR8.2 dvpr (Addgene plasmid #8455), pCMV-VSV-G (Addgene plasmid #8454 ...
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bioRxiv - Neuroscience 2022Quote: Adult male MC4Rlox/lox mice[10] were injected with were injected with pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (Addgene Cat. No. 105545-AAV9) into IL (bregma +0.175 AP ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg of pMDLg/p RRE and 10 μg of pRSV-Rev packaging plasmids (Addgene #12251 and Addgene #12253) and the pVSV envelope plasmid (Addgene #8454 ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg of pMDLg/p RRE and 10 μg of pRSV-Rev packaging plasmids (Addgene #12251 and Addgene #12253) and the pVSV envelope plasmid (Addgene #8454 ...
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bioRxiv - Cell Biology 2021Quote: ... and co-transfected at DIV 10 with the mCherry-GFP-LC3 reporter (gift from Anne Brunet, Addgene plasmid # 110060) and either α-S824 or myc-β4 protein ...
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bioRxiv - Immunology 2021Quote: ... A plasmid expressing SP140 was made by cloning the purified SP140 cDNA into the p3xFLAG-CMV-10 vector (Addgene) using Gateway techniques (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV injections consisted of a 1:10 cocktail of Cre [AAV5-CMV-HI-eGFP-Cre.WPRE.SV40 (Addgene plasmid no. 105545) packaged at UPenn Vector Core 2.5 × 1014 GC ml−1] and KORD [AAV8-HSyn-DIO-HA-KORD-IRES-mCitrine (Addgene plasmid no ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of AAV9.hsyn.FLEX.iGluSnFR.WPRE.SV40 (a gift from Loren Looger, Addgene plasmid # 98931; http://n2t.net/addgene: 98931; RRID:Addgene_98931, 10 vg/mL in water) solution was injected with a blunt tip (30 gauge ...
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bioRxiv - Microbiology 2023Quote: ... cerevisiae BY4741 as described in the methods of Bao.10 The pCRCT plasmid was a gift from Huimin Zhao (Addgene plasmid # 60621; http://n2t.net/addgene:60621; RRID: Addgene_60621) and purchased from Sigma.
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bioRxiv - Neuroscience 2023Quote: Adult mice were stereotaxically injected at 7-10 weeks of age with 600 nl of 1.5×1013 vg/ml AAV1-CKIIa-stGtACR2-FusionRed (AddGene) or 7×1012 vg/ml AAV1-CKIIa-mCherry control at bilateral ACC (antero-posterior (AP ...
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bioRxiv - Neuroscience 2023Quote: ... a mixture of flexed AAV GFP (pAAV-FLEX-GFP-Virus, titer ≥ 1×10¹³ vg/mL, Addgene 28304-AAV PHPeB) and CamKII-Cre (pENN.AAV.CamKII 0.4.Cre.SV40 ...
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bioRxiv - Molecular Biology 2023Quote: ... AAVpro 293T cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% (v/v) FBS (NICHIREI) and transfected with the pUCmini-iCAP-PHP.eB plasmid (Addgene), pHelper plasmid (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-hSyn-DIO-hM4D(Gi)-mCherry or AAV5-hSyn-DIO-mCherry (all viruses: 4.2*10^12 gc/ml; Addgene) in the LC (AP ...
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bioRxiv - Neuroscience 2023Quote: ... After 2 weeks the same procedure was used to injected 200nL of AAV5-hSyn1-SIO-eOPN3-mScarlet-WPRE (3,4 10^12 gc/mL Addgene) at the same previous locations inside the RSC ...
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bioRxiv - Neuroscience 2024Quote: ... Each eye was injected with 1μL of AAV2-Ef1a-fDIO-mCherry (7×10¹² vg/mL; Addgene, Catalog #: 114471-AAV2) using a custom Hamilton syringe (Borghuis Instruments ...
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bioRxiv - Immunology 2024Quote: ... Mayo Clinic) and 10 μg of TCR-2A-OTI-pMIG-II DNA (gift from Dr. Dario Vignali, Addgene #52111) as described above ...
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bioRxiv - Neuroscience 2024Quote: ... each mouse was transduced with a cocktail of CRE recombinase-dependent AAV vectors to express Brainbow 3.0 (AAV-EF1a-BbTagBY and AAV-EF1a-BbChT; titers ≥ 1×10¹³ vg/mL; Addgene) 60 under isoflurane anesthesia ...
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bioRxiv - Microbiology 2024Quote: ... before co-transfection the next day with 15 µg of the pLVX-3xHA-TurboID-RAB11A plasmid along with 10 and 5 µg of the pΔ8.74 (Addgene #22036) and pMD2.G (Addgene #12259 ...
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bioRxiv - Developmental Biology 2021Quote: ... were seeded in DMEM with 10% FBS and transfected with the lentivirus vectors along with pCMV-VSV-G (Addgene, 8454), pRSV-Rev (Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... A pulled glass micropipette attached to a nanoinjector (MO-10, Narishige) was used to inject 400nL of pGP-AAV9-syn-FLEX-jGCaMP7s-WPRE (Addgene) virus at a depth of 4.8mm DV at a rate of 100 nL/min ...
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bioRxiv - Cell Biology 2022Quote: ... 5.25 μg of transfer plasmid (LentiGuide-Hygro with 10-target or 20-target mgRNA) was mixed with 0.75 μg of pMD2.G (Addgene #12259) and 1 μg of psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2022Quote: ... Vectors carrying 10-target or 20-target mgRNAs were made by cloning mgRNAs into the LentiGuide-Hygro plasmid (Addgene #139462). In short ...
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bioRxiv - Neuroscience 2020Quote: ... a series of 6 - 10 microinjections (50 - 100 nL each, 200-300 μm depth) of AAV5.Flex.ArchT.tdTomato (Addgene, #28305-AAV5) were delivered along the posterior to anterior axis of RSC (2.0 to 4.0 mm posterior ...
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bioRxiv - Microbiology 2021Quote: ... 10 μg of pEA216-1 was then co-transfected with 5 μg of the pCMVΔR8.2 helper plasmid (Addgene Plasmid #122263) and 1 μg of pHEF-VSV-G into 70% confluent monolayers of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc ...
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bioRxiv - Molecular Biology 2022Quote: SVF cells were isolated as above and on the same day a confluent 10 cm dish of 293T cells were transfected with 1.5 µg psPAX2 lentiviral packaging vector (Addgene 12260), 1.5 µg pMD2.G envelop plasmid (Addgene ...
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bioRxiv - Microbiology 2022Quote: HEK293T seeded in 10-cm dishes were co-transfected with lentiviral packaging plasmid psPAX2 (gift from Didier Trono, Addgene #12260), lentiviral vector pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase (gift from Ethan Abela ...
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bioRxiv - Neuroscience 2020Quote: ... AAV1.hSyn.GCaMP6f.WPRE.SV40 (120 nl, 2×1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 to 1:10 in saline) was injected either into V1 ...