Labshake search
Citations for Addgene :
301 - 350 of 352 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: AAV-SYN-flex-PSAM4-GlyR-IRES-EGFP was a gift from Scott Sternson (Addgene viral prep # 119741-AAV5; http://n2t.net/addgene:119741; RRID:Addgene_119741; >1×1013 vg/ml). Control virus was AAV1-EF1α-DIO-eYFP (>1×1013 vg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... Following AAVs were used: AAVrg-CAG-FLEX-rc[Jaws-KGC-GFP-ER2] (titer >7×1012 vg/ml, viral prep #84445-AAVrg, Addgene, US,34) or AAVrg-hSyn-DIO-hM3D(Gq)-mCherry (titer >7×1012 vg/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... and AAV9 ef1a-flex-Tdtomato viral vectors were purchased from the Canadian Neurophotonics Platform Viral Vector Core Facility (RRID:SCR_016477) and AAVrg-flex-Tdtomato was purchased from Addgene (viral prep: 28306-AAVrg). Retrogradely infecting AAVs were used for brain injections while AAV9 was used for intravitreal injections.
-
bioRxiv - Neuroscience 2022Quote: ... an injection of AAV1 carrying a fused channelrhodopsin2-YFP protein (AAV1-hSyn-ChR2-H134R-eYFP- WPRE, Addgene, viral prep no. 26973-AAV1) was placed into either MEC ...
-
bioRxiv - Neuroscience 2022Quote: ... carrying the GCaMP6s or GCaMP7s transgenes (pAAV.Syn.GCaMP6s.WPRE.SV40 or pGP-AAV-syn-jGCaMP7s-WPRE) purchased from Addgene (viral preps #100843-AAV1, 104487-AAV1) with titers of 1-5×1012 in dorsal dentate gyrus using a Nanoject syringe (Drummond Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... were achieved using AAV8-Syn-Chronos-tdTomato-WPRE-bGH (pAAV-Syn-Chronos-tdTomato was a gift from Edward Boyden (Addgene viral prep # 62726-AAV8 ; http://n2t.net/addgene:62726 ; RRID:Addgene_62726, (Klapoetke et al., 2014). Inhibitory DREADD expression was induced in a Cre-dependent manner using AAV9-hSyn-DIO-hM4D(Gi)-mCherry (pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV9 ...
-
bioRxiv - Neuroscience 2022Quote: ... In vivo calcium imaging experiments were performed using AAV1-hSyn-GCaMP6s.WPRE.SV40 (pAAV.Syn.GCaMP6s.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep #100843-AAV1; http://n2t.net/addgene:100843; RRID:Addgene_100843, (Chen et al., 2013), AAV9-hSyn-FLEX-axonGCaMP6s (pAAV-hSynapsin1-axon-GCaMP6s was a gift from Lin Tian (Addgene viral prep # 112010-AAV9 ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV9-hSyn-FLEX-axonGCaMP6s (pAAV-hSynapsin1-axon-GCaMP6s was a gift from Lin Tian (Addgene viral prep # 112010-AAV9 ; http://n2t.net/addgene:112010 ; RRID:Addgene_112010, (Broussard et al., 2018) and AAV1-CAG-tdTomato (pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene viral prep # 59462-AAV1 ...
-
bioRxiv - Neuroscience 2022Quote: ... Inhibitory DREADD expression was induced in a Cre-dependent manner using AAV9-hSyn-DIO-hM4D(Gi)-mCherry (pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV9; http://n2t.net/addgene:44362; RRID:Addgene_44362, (Krashes et al., 2011). Fluorescent protein mCherry expression was induced in a Cre-dependent manner using AAV5-hSyn-DIO-mCherry (pAAV-hSyn-DIO-mCherry was a gift from Bryan Roth (Addgene viral prep # 50459-AAV5 ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV9-EF1a-DIO-hChR2-EYFP (1:2, Addgene viral prep # 20298- AAV9; http://n2t.net.addgene:20298; RRID; Addgene:20298, gift from Karl Deisseroth); Fig 4 ...
-
bioRxiv - Neuroscience 2024Quote: ... USA (pAAV5-CaMKIIa-hM4D(Gi)-mCherry and pAAV5-CaMKIIa-hM3D(Gq)-mCherry were gifted from Bryan Roth (Addgene viral prep # 50477and # 50476; pAAV-CaMKIIa-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 114469). Viral constructs were used as is or diluted in saline before use ...
-
bioRxiv - Neuroscience 2021Quote: ... and four mice (control group) were stereotactically injected in the DR with AAV2/5-EF1α-DIO-WPRE-eYFP (Addgene viral prep 27056-AAV5) at a rate of 75 nl per min using surgical procedures (anesthesia ...
-
bioRxiv - Microbiology 2023Quote: ... cells were transduced with the respective lentivirus (pWPI-IRES-Puro-Ak-ACE2, kindly provided by Dr. Sonja Best, Addgene viral prep # 154985-LV) at MOI 0.3 ...
-
bioRxiv - Neuroscience 2024Quote: ... and 80-100nl of either AAV5-hSyn-hChR2(H134R)-EYFP (UNC Vector core) or pAAV9-mDlx-ChR2-mCherry-Fishell-3 (Addgene viral prep # 83898) was injected into the right GPi or SNr ...
-
bioRxiv - Neuroscience 2020Quote: ... Dissociated single cell forebrain NPCs were plated 1,000,000 cells/well on 12 well plates and transfected with lentiGuide-tdTomato (Addgene #99376) plasmid and selected by hygromycine ...
-
bioRxiv - Neuroscience 2023Quote: ... After 2 weeks the same procedure was used to injected 200nL of AAV5-hSyn1-SIO-eOPN3-mScarlet-WPRE (3,4 10^12 gc/mL Addgene) at the same previous locations inside the RSC ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV5-hSyn-DIO-hM4D(Gi)-mCherry or AAV5-hSyn-DIO-mCherry (all viruses: 4.2*10^12 gc/ml; Addgene) in the LC (AP ...
-
bioRxiv - Neuroscience 2022Quote: ... The viral vector AAV1-hSyn-GCaMP7f (pGP-AAV-syn-jGCaMP7f-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 104488-AAV1)) was injected and a titanium anchor was attached using dental cement (C&B Metabond ...
-
bioRxiv - Bioengineering 2023Quote: ... was transfected with 1 µL AAV1-hSyn1-GCaMP6f-P2A-nls-dTomato virus (Addgene viral prep #51085-AAV1, http://n2t.net/addgene:51085, RRID:Addgene 51085, a gift from Jonathan Ting) diluted 1 ...
-
bioRxiv - Neuroscience 2022Quote: ... or a mixture consisting of equal amounts of AAV1-CAG-FLEX-tdTomato and AAV1-CamKII- eGFP-WPRE-rBG (Addgene, viral prep no. 105541-AAV1). To specifically label SST+ neurons in LEC ...
-
bioRxiv - Systems Biology 2020Quote: ... coli K-12 MG1655 Δcrp genome was done using a two-step recombination method using pSLTS plasmid (Addgene plasmid #59386). The primers used in this method are given in Supplementary Table S2 ...
-
bioRxiv - Neuroscience 2021Quote: ... unilaterally with 70 nl viral mixture of AAV pCAG-FLEX-{EGFP}on (Addgene plasmid #51502, titer 1.1E+12 GC/ml,) and AAV-{CAR}off (titer 1E+12 GC/ml)104 at 200 µm depth from the bulb surface ...
-
bioRxiv - Genomics 2022Quote: ... 12 μg of plasmid library was cotransfected with 9 μg psPAX2 and 3 μg pMD2.G (Addgene #12260 and #12259) into HEK293FT cells using 72 μl Lipofectamine 2000 (Life Technologies #11668019) ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T-LentiX cells were co-transfected for 12 h with 10 µg psPAX2 (kind gift from Didier Trono, Addgene #12260), 2.5 µg pMD2.G (kind gift from Didier Trono ...
-
bioRxiv - Neuroscience 2024Quote: ... -2.2 mm ventral from skull under an angle of 10°) and injected with AAV5-hSyn-DIO-mCherry (3*10^12 gc/ml; 300nl; Addgene) in LHA (-1.3 mm posterior to bregma ...
-
Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: Cells were seeded in 12-well plates and transfected at 50-70% confluence with 0.5 µg/well TOPflash (Addgene, #12456) and 0.3 µg pRL-TK (Promega ...
-
bioRxiv - Neuroscience 2024Quote: ... Banghart Lab) or control AAV5-hSyn1-DIO-EGFP (AAV-EGFP; titer: 1.30E+12 GC/mL, Addgene, cat. no. 50457-AAV5). Following surgery ...
-
bioRxiv - Neuroscience 2024Quote: AAV used in the study were as followed: AAV2-hSyn-DIO-hM3D(Gq)-mCherry (Titer: 6e-12 vg/mL) was a gift from Bryan Roth (Addgene plasmid #44361 ...
-
bioRxiv - Neuroscience 2024Quote: ... to express the genetically encoded calcium indicator GCaMP under the pan-neuronal synapsin promoter (AAV1-Syn-jGCaMP7f- WPRE, AddGene, viral prep 104488-AAV1, titer 2x1012). The needle was left in place for 10 min to allow for virus diffusion.
-
bioRxiv - Neuroscience 2024Quote: ... rats were anesthetized with isoflurane for bilateral infusion of ACC with either an inhibitory opsin archaerhodopsin T (AAV9-CaMKII-ArchT)-Green (Addgene, Cambridge, MA, viral prep #99039) or eYFP (AAV9-CamKII-EYFP ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 million cells per replicate were activated and 12-20 h later electroporated with the pSpCas9(BB)-2A-GFP plasmid (pX458; Addgene 48138) expressing the Myc sgRNA using the MaxCyte STx transfection system (MaxCyte) ...
-
bioRxiv - Neuroscience 2021Quote: ... 8-12 weeks old Ucn3::Cre male mice were stereotaxically injected with pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, Cambridge, MA) bilaterally into the PeFA (AP −0.6 ...
-
bioRxiv - Biophysics 2021Quote: ... were transfected in DMEM with 2 % (v/v) FBS with 12 μg of pHR-CMV-TetO2 construct vector alongside 11 μg envelope plasmid (pMD2.G; Addgene #12259) and 11 μg packaging plasmid (psPAX2 ...
-
bioRxiv - Microbiology 2021Quote: ... 2 x 105 cells were seeded on 12-well plates and transiently transfected with 2 μg of plasmids encoding SARS-CoV-2 N (Addgene # 141391) or eGFP (Addgene # 141395 ...
-
bioRxiv - Cell Biology 2020Quote: ... Primers containing this targeting sequence (primers 11 and 12) were annealed and subcloned into the pU6-(BbsI) CBh-Cas9-T2A-mCherry vector (Addgene #64324) digested with BbsI.
-
bioRxiv - Neuroscience 2020Quote: ... while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA), All animals received three viral injections in the anterior cingulate cortex in each hemisphere as follows (skull at +5.0mm to horizontal plane) ...
-
bioRxiv - Neuroscience 2020Quote: ... and the control group (n = 10) received injections of AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA). The injection coordinates and volumes for the three injections made into the anterior cingulate cortex were as follows ...
-
bioRxiv - Molecular Biology 2023Quote: ... Repeat sequences of (TTAGGG)23 and (TTACCG)23 were synthesized by GeneScript and subcloned into pHR-Tre3G-29×GGGGCC-12×MS2 (Addgene #99149) using Ligation high (TOYOBO) ...
-
bioRxiv - Cell Biology 2024Quote: ... control or mir-218-1-overexpressing HTR8 cells were seeded into 12-well plates and were co-transfected with 1 µg/mL of 5X NFκB luciferase reporter (64) (Addgene plasmid #12453) construct and 20 ng/mL of Renilla luciferase vector (Promega ...
-
bioRxiv - Systems Biology 2022Quote: ... Transfection with siRNA or the RFP-tagged protein of interest was performed 12 hours prior to transfection with I-SceI (Addgene #26477) or GFP control (Addgene #89684) ...
-
bioRxiv - Cell Biology 2023Quote: ... The guide RNA targeting sequence 5’- TGGTCGTGGATACGAGAAGA-3’ was inserted into the Peft-3>Cas9 + sgRNA plasmid pDD162 [12] (Addgene #47549) using a Q5 site-directed mutagenesis (New England Biolabs) ...
-
bioRxiv - Neuroscience 2021Quote: ... Black-6 mice were first injected with 120nL of a retrograde virus encoding Cre (AAVrg-Ef1a-mCherry-IRES-Cre, titer of 1.37 x 10^13, Addgene viral prep # 55632-AAVrg, provided to Addgene by Karl Deisseroth (Fenno et al., 2014)) in either the central lateral nucleus (CL ...
-
bioRxiv - Neuroscience 2022Quote: ... Three injections of AAV9-rTH-PI-Cre-SV40 (functional, titer >7×1012 vg/ml, viral prep #107788-AAV9, Addgene, US, a gift from James M. Wilson) or were made analogously to spinal injections ...
-
bioRxiv - Neuroscience 2022Quote: ... Recombinant AAV9-hEF1a-LoxP-TagBFP-LoxP-eYFP-LoxP-WPRE-hGH-InvBYF and AAV9-hEF1a-LoxP-mCherry-LoxP-mTFP-LoxP-WPRE-hGH-InvCheTF viruses were a gift from Dawen Cai & Joshua Sanes (Cai et al. 2013) and obtained from the University of Pennsylvania Vector Core (Addgene viral preps # 45185-AAV9; # 45186-AAV9). A total of ∼9 × 108 to 2 × 109 viral genome particles of each vector (∼0.25 µL of 3-4 × 1012 GC per mL dilution in sterile PBS with 0.02% Fast Green FCF dye ...
-
bioRxiv - Genetics 2020Quote: ... Male mice at ages 8-12 weeks were Jugular vein injected with 1011 particles of AAV expressing either Cre recombinase (Addgene 107787-AAV8) or GFP (Addgene 105535-AAV8 ...
-
bioRxiv - Neuroscience 2024Quote: ... Gi DREADD virus (n=25,13 males, 12 females: AAV8-hSyn-DIO-hM4Di-mCherry,≥ 1×101 3 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express inhibitory designer receptors in VP GABA neurons ...
-
bioRxiv - Neuroscience 2022Quote: ... we injected 300-500 nL of AAV5-CAG-Flex-GCamp6f-WPRE-SV40 (University of Pennsylvania Vector Core or Addgene, ∼8.75E+12 parts/mL) into the ACC at a depth of 1.5mm ...
-
bioRxiv - Genetics 2024Quote: We use two constructs for epigenome editing: 1) dCAS9-DNMT3A/3L co-expressed with enhanced green fluorescent protein (eGFP) for selection 12 (Addgene, Catalogue number 128424), and 2 ...
-
bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
-
bioRxiv - Bioengineering 2023Quote: ... the neurons were transduced with 1 µL of an adeno-associated virus serotype 9 (AAV9) carrying a fluorescent calcium ion indicator GCaMP6s under a pan-neuronal human synapsin (hSyn) promoter (AAV9-hSyn::GCaMP6s, Addgene viral prep #100843-AAV9, >1×1013 IU/µL). After 5 days of incubation ...