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Citations for Addgene :
301 - 350 of 867 citations for Hantaan Virus Nucleoprotein HEK293 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... or AAV5-EF1a-DIO-eYFP (Addgene #27056; 1.3 x 1013 virus molecules/ml) in the MPO to express the opsin Channelrhodopsin2 (hChR2 ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV5-EF1a-DIO-eYFP (Addgene #27056; 1.3 x 1013 virus molecules/ml) in the MPO of NtsVGAT-/- to express ChR2 or eYFP ...
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bioRxiv - Neuroscience 2024Quote: ... of a Cre-dependent virus encoding hM3Dq (AAV5- hSyn1-DIO- hM3Dq-mCherry, Addgene) or mCherry alone as control (AAV5-hSyn1-DIO- mCherry ...
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bioRxiv - Cell Biology 2019Quote: We generated Schizosaccharomyces pombe strains with SNAP-tag (Addgene plasmid 87024) or mEGFP (Addgene plasmid 87023 ...
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bioRxiv - Biochemistry 2023Quote: ... or N-terminal His10-MBP Tag (2CT-10; Addgene Plasmid #37237) were chosen as the MBP tag has been shown to increase the solubility of select proteins [9] ...
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bioRxiv - Plant Biology 2023Quote: ... a C-terminal 3×FLAG® epitope tag (pICSL50007; Addgene #50308), and 3′ UTR and terminator sequences from Agrobacterium tumefaciens nopaline synthase (AtuNOST ...
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bioRxiv - Cell Biology 2022Quote: ... The dual-tag Galectin3 plasmid was acquired from Addgene (ref. 64149). The TBC1D15 coding sequence was subcloned into vectors pEGFPC1 and pGEX6P1 for expression in mammalian and bacterial cells ...
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bioRxiv - Cell Biology 2024Quote: Plasmid containing full length YAP1 with T2A-mCherry tag (#74942, Addgene) was transfected together with envelope expressing plasmid 9 - PMS2.G (#12259 ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Microbiology 2019Quote: ... GM-CSF and IL-4 were produced from HEK293 cells transduced with pAIP-hGMCSF-co (Addgene #74168) or pAIP-hIL4-co (Addgene #74169) ...
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bioRxiv - Microbiology 2019Quote: ... These cytokine-conditioned media were produced from HEK293 cells stably transduced with pAIP-hGMCSFco (Addgene no. 74168) or pAIP-hIL4-co (Addgene no ...
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bioRxiv - Cell Biology 2022Quote: ... we co-transfected HEK293-FT cells with plasmids of interest with VSVG (pMD2.G; Addgene plasmid 12259) and psPAX2 (Addgene plasmid 12260 ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid for expression of EPS15-GFP-His was obtained from Addgene (#170860). EPS15D177S was generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Biochemistry 2022Quote: pQE-60 roGFP2-His was a gift from Tobias Dick (Addgene #65046)(50) ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... pVitro-hygro-N-myc-hVps34/Vps15-C-V5-his-plasmid (Addgene #24055), pEGFP-Atg14L (Addgene #21635 ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for expression of His-ULP1 was from Addgene (Plasmid #64697), the His-Sortase plasmid was created as previously described 4 ...
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bioRxiv - Genetics 2021Quote: Mice were injected with Cre-expressing adeno-associated virus AAV1.hSyn.Cre.WPRE.hGH (105553-AAV1, Addgene) and GFP-expressing AAV1.hSyn.eGFP.WPRE.bGH (105539-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: The commercial AAV5-hSynhM3D(Gq)-mCherry or the control virus AAV5-hSyn-mCherry (Addgene) were injected into the sciatic nerve of WT C57BL/6J mice (1μL/sciatic nerve ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transduced with the following virus: pTet-O-NGN2-puro (Addgene #52047): 0.1 μL per 5×104 cells ...
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bioRxiv - Neuroscience 2019Quote: ... A control virus (AAV5-CamKIIα-eGFP, 4.1×1012) (Cat No. 50469-AAV5; Addgene, USA) was administered into the contralateral left EC using identical parameters ...
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bioRxiv - Neuroscience 2022Quote: ... or a control virus AAV9-hSyn-eYFP (Addgene, viral titer 2.8 × 1013 vg/mL) was injected in the NAcSh of WT mice while AAV9-Syn-Flex-GcAMP6f-eYFP (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... The TauRD(P301S)-YFP plasmid DNA with virus packing pDNAs (psPAX2; pMD2.G, Addgene) was transfected into 293T cells by 1mg/mL PEI transfection reagent and incubated for 36 hours ...
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bioRxiv - Physiology 2024Quote: AAV9 virus expressing jGCaMP8m under a CAG promoter was obtained from Addgene (162381-AAV9). For injections ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth ...
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bioRxiv - Neuroscience 2024Quote: ... Animals used for RSC imaging received a mixture of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (Addgene: 100837), AAV1.cFos-tTA and AAV1.TRE.mCherry at 20 to 120 nL/min into dorsal cortex using stereotactic coordinates −1.7 and −2.3 mm posterior to bregma ...
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bioRxiv - Cancer Biology 2024Quote: HT29 cells previously infected with pLenti-U6-tdTomatato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and TdTomato were used ...
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bioRxiv - Cancer Biology 2024Quote: ... the shRNA constructs were packaged into second-generation virus particles using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2023Quote: ... iAChSnFR and iAChSnFR-Null constructs and AAV virus are available from Addgene (137950–137962).
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bioRxiv - Microbiology 2023Quote: ... a vesicular stomatitis virus G (VSV-G) envelope expression vector (pMD2.G, #12259, Addgene) and a lentiviral transfer vector containing the transgene in a ratio of 2:1:3 by calcium phosphate transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of a virus encoding AAV5.CamKIIa.GCaMP6f (pENN.AAV.CamKII.GCaMP6f.WPRE.SV40, Addgene viral prep 100834-AAV5) virus was injected unilaterally in the PL (AP +1.9 ...
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bioRxiv - Neuroscience 2023Quote: Birds were injected with the AAV9-CAG-GCaMP6f-WPRE-SV40 virus (100836-AAV9, Addgene). The total amount of virus injected was 897 nL (65 injections of 13.8 nL each) ...
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bioRxiv - Neuroscience 2024Quote: ... Channelrhodopsin (ChR2) expressing virus (pAAV9-hSyn-hChR2(H134R)-mCherry) (AddGene, 26976-AAV9, Watertown, MA) and Retrobeads™ (Red RetroBeads ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were injected with adeno-associated virus (AAV-PHP.eB-syn-jGCaMP7s, Addgene) using a 30-gauge needle ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondria were labelled in cells by transduction with pLV-mitoDsRed virus (Addgene, Plasmid 44386) harvested post expression in 293-FT cells and lentiviral particle production ...
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bioRxiv - Neuroscience 2024Quote: ... The retrograde adeno-associated virus (AAV) tracer (AAVrg pAAV-Ef1a-DIO EYFP, Addgene#27056) was infused (∼50 nL/side ...
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bioRxiv - Neuroscience 2021Quote: ... CalR-HaloTag-Snap-tag-KDEL (ER-localizing) or GCaMP6f (Addgene 100837-AAV1). On day 14-18 the neurons were incubated for at least 2 h with 1 μM of the corresponding MaPCa dyes containing 0.04% Pluronic-F127 ...
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bioRxiv - Biochemistry 2019Quote: ... coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Biophysics 2022Quote: ... The His6-SUMO tag was subsequently cleaved with human SenP1 (Addgene #16356) 27 and separated on Ni2+-Histrap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: GST-tag human HP1⍺ was a kind gift from Naoko Tanese (Addgene plasmid # 24074 ...
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bioRxiv - Genomics 2023Quote: ... Mito-tag plasmids were based on pMXs-3XHA-EGFP-OMP25 (Addgene, #83356) and pMXs-3XMyc-EGFP-OMP25 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg shRNA p53-puromycin (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells grown in twelve 10 cm dishes were co-transfected with active myc-mTOR E2914K mutant (Addgene) and HA-Raptor (Kim et al. ...
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bioRxiv - Cell Biology 2021Quote: Measurement of NFAT4 nuclear translocation in HEK293 cells was achieved by transient overexpression of NFAT4-GFP (Addgene #21664) as previously described(30 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was generated by co-transfection of HEK293 cells with viral vector and packaging plasmids psPAX2 (Addgene 12260) and pMD2.G (Addgene 12259 ...