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Citations for Addgene :
251 - 285 of 285 citations for pEGFP C2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: We PCR amplified Ecad using pEGFP-N1-Ecad plasmid [46] and V5-TurboID using mutant BirA R118S (TurboID) (Addgene [17]) using PCR primers (Table S4) ...
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bioRxiv - Cancer Biology 2020Quote: The full-length construct of Ubiquitin (HA-Ubiquitin Plasmid #18712) and Nup88 (pEGFP-Nup88 Plasmid #64283) were obtained from Addgene. The C-terminal coiled-coil domain (Nup88-C ...
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bioRxiv - Neuroscience 2020Quote: Full-length Susd4 mouse gene was cloned into the mammalian expression vector pEGFP-N1 (Addgene, Massachusetts, USA, Cat#6085-1) to express a SUSD4-GFP fusion construct under the control of the CMV promoter (pSUSD4-GFP) ...
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bioRxiv - Cancer Biology 2021Quote: ... GFP and GFP-tagged K19 WT and mutantconstructs were cloned from pEGFP-C3 K19 constructs into pLenti CMV Hygro (plasmid #17484. Addgene) using the primers listed in Table 1 with the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... EGFP-Miro2 and mCherry-Miro1 were generated by PCR amplification of the coding sequence of Miro and ligated into pEGFP-C1(Addgene) or pmCherry-C1(Addgene) ...
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bioRxiv - Cell Biology 2021Quote: The EB1-GFP lentivirus expression construct was made by PCR amplification of EB1-GFP from pEGFP N1 EB1-GFP (JB131) (gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39299 ...
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bioRxiv - Immunology 2020Quote: ... and the full-length protein (IFT20, aa 1-132) in-frame with the tags into the pEGFP-N1 (#6085-1 Addgene) and pGEX-6P-2 vectors (#27-4598-01Addgene) ...
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bioRxiv - Microbiology 2022Quote: ... pEGFP-N1-TFEB (38119) and pEGF-N1-Δ30TFEB (44445) were obtained from Addgene (a generous gift of Shawn M. Ferguson). pEGFP-2xFYVE (140047 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: SH-SY5Y cells were plated in 6-well plates at a density of 400,000 cells per well and transfected the following day with pEGFP-n1-APP (69924, Addgene plasmid) using the jetOPTIMUS transfection reagent (Polyplus ...
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bioRxiv - Molecular Biology 2023Quote: We generated the construct: pcDNA3.1-EGFR-flag-APEX2 by amplifying the sequence encoding EGFR from the plasmid pEGFP-N1-EGFR-EGFP (Addgene: #32751) and cloning it into pcDNA3.1 (+ ...
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bioRxiv - Cell Biology 2024Quote: ... 100,000 HeLa M cells were plated per well in a 6-well plate and transfected the following day at a ratio of 1:15 with the pEGFP-Puro plasmid (45561; Addgene) and gRNA-containing plasmid (pX330A-1×2) ...
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bioRxiv - Cancer Biology 2021Quote: ... wildtype or truncated CD95 sequence was extracted either from 5UTR-SP-HA-CD95 wt (1-319)-eGFP peGFP-N1 or 5’UTR-SP-HA-CD95 wt (1-175)-eGFP peGFP-N1 by NdeI and SmaI and inserted into the NdeI and AfeI sites of pMCS-BioID2-HA (Addgene #74224). The plasmid encoding the CD95 transmembrane domain fused in frame to the BirA was created by inserting the following annealed primers into the EcoRI and BamHI site of pMCS-BioID2-HA ...
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bioRxiv - Cell Biology 2020Quote: ... containing human ATF6 cDNA fused to mCerulean on the C-terminal side of ATF6 was generated by amplifying ATF6 ORF from pEGFP-ATF6 (Addgene #32955) by PCR using the following primers ...
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bioRxiv - Neuroscience 2020Quote: Transfection for the CD63 overexpression construct was done as described above for siRNA transfection using 2 µg/mL of CD63-pEGFP (Addgene, USA) added for 5 h in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product was digested with EcoRI and BamHI restriction enzymes and sub-cloned into EcoRI/BamHI-digested pEGFP-C1 plasmid (Addgene #2487).
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bioRxiv - Cell Biology 2020Quote: Plasmids used for transfection include pEGFP-C1 (GenBank: U55763.1, Clonetech) and pcDNA3-EGFP-Rac1-Q61L (a gift from G. Bokoch, Addgene plasmid #12981) (Subauste et al. ...
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bioRxiv - Microbiology 2022Quote: GFP and myc-tagged 14-3-3ε were generated as follows: a G-block containing the full-length 14-3-3ε protein (IDT) was cloned into pEGFP-C1 (Addgene #2487) using XhoI and BamHI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: An insert of ZZUD and ZZUDL1340P were cloned out from a positive pGEX-6P-1-ZZUD or pGEX-6P-1 - ZZUDL1340P with PCR based cloning procedure into a pEGFP-C1 (Addgene 54759) or mRFP-C1 (Addgene 54764 ...
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bioRxiv - Immunology 2022Quote: ... pTRIP-SFFV-Hygro-2A-mScarlet-VPS4A E228Q was obtained by Gibson assembly of PCR amplified VPS4A E228Q from pEGFP-VPS4-E228Q (Addgene #80351). pTRIP-SFFV-Hygro-2A-mScarlet-UBAP1DN and pTRIP-SFFV-Puro-2A-mScarlet-UBAP1DN (truncated mutant at residue 97 - mutation G98X ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-C1-tagged plasmids containing the exon 1 of HTT with 23 CAG repeats (GFP-Q23: wild-type HTT. Addgene, #40261) or 74 CAG repeats (GFP-Q74 ...
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bioRxiv - Biochemistry 2022Quote: ... corresponding to amino acids 629-633 of AR) of the eGFP-AR fusion protein73 was removed from peGFP-C1-AR (Addgene #28235) using the Q5 site-directed mutagenesis kit and primer design tools (New England BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... the transgene p130PH or p130PHR134L were amplified using PCR from the pEGFP-N1 plasmids containing these genes31 and inserted into the Ef1α-DIO-mCherry viral vector (Addgene, #47636). AAVTM6 viruses were produced at Janelia Viral Tools facility ...
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bioRxiv - Neuroscience 2023Quote: ... adult DRG neurons from Twitcher and WT mice were isolated as described above and nucleofected with a plasmid encoding pEGFP-eEB3 (Addgene #190164). After transfection ...
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bioRxiv - Cell Biology 2023Quote: ... mCherry-iLID was amplified using 5’-GGTAGTAGTGGTAGTAGTATGGTGAGCAAGGGCGA-3’ and 5’-TCGAAGCTTGAGCTCGAGATCTTTAAAAGTAATTTTCGTCGTTCGCT-3’.The fragments were inserted into a pEGFP-C1 vector (Addgene 46956) using the AgeI/BglII restriction sites.
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pEGFP-LC3 (EGFP-LC3: microtubule associated protein 1 light chain 3 fused with enhanced green fluorescent protein) (Addgene, 24920) at 0.1 µg of the plasmid (in 50 µl Opti-MEM® Reduced Serum Media ...
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bioRxiv - Immunology 2021Quote: ... GFP-YVAD was PCR-amplified from pEGFP-C3 and cloned into the Lamp1-RFP plasmid (a gift from Walter Mothes; RRID: Addgene Cat.#1817) (Sherer et al. ...
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bioRxiv - Cell Biology 2021Quote: ... human MCOLN1 was amplified by PCR with the following oligonucleotides with overhangs (underlined): 5’-GACACCGACTCTAGAATGGTGAGCAAGGGCGAGGAGC-3’ (forward) and 5’-AACTAGTCCGGATCCTCAATTCACCAGCAGCGAATGC-3’ (reverse) from Mucolipin1-pEGFP C3 (Addgene plasmid #62960) construct and subcloned into XbaI and BamHI restriction sites of pLenti-CMV-MCS-GFP-SV-puro (Addgene plasmid #73582 ...
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bioRxiv - Cell Biology 2020Quote: ... shRNA containing vectors were constructed by ligation of shRNA sequence between the EcoRI and PmeI sites downstream of the U6 promoter of modified pEGFP-U6 vector 36 (a gift from Zuoshang Xu, Addgene plasmid #19799). Target sequence for human MARCKSL1 is 5’-GTGTGAACGGAACAGATGATG-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... CMV-EGFP-FRB-EEA1 was generated by site-directed mutagenesis to remove the stop codon upstream of FRB from pEGFP-FRB (Addgene, Cat #25919). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 × 106 cells of each cell line were electroporated with a mixture of 10 μg of plasmid pEGFP-D50 lamin A (Addgene plasmid #17653) that had been linearized with EagI and 1 μg of pBABE-puro (Addgene plasmid #1764 ...
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bioRxiv - Molecular Biology 2024Quote: ... with HA-tag at the N-terminal and GFP-tag at the C-terminal were cloned in pEGFP-N3 expression vector (Addgene #6080-1) (Table S8 ...
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bioRxiv - Biochemistry 2024Quote: Full-length VCPIP1 in pDEST_CMV with a N-terminal Strep tag was acquired from Wade Harper 42 and VCP was a gift from Nico Dantuma (VCP in pEGFP-N1 [Addgene plasmid #23971]). From there ...
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bioRxiv - Cell Biology 2022Quote: ... pEGFP-N1-TFEB plasmid was a gift from Shawn Ferguson (Addgene plasmid # 38119; http://n2t.net/addgene:38119; RRID:Addgene_38119n (Roczniak-Ferguson et al., 2012)) or EGFP-2X FYVE plasmid (kind gift from B ...
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bioRxiv - Cell Biology 2023Quote: ... C-tRFP-Lck (cloned into PCMV6-AC-RFP expression vector) and TagRFP-T-EEA1 (cloned into pEGFP-C1 vector) were purchased from Addgene (respectively #RC100049 and #42635). Strawberry-βarr2 was a gift from Prof ...