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Citations for Addgene :
251 - 300 of 601 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Bot oligo – 5’ aaacGGGTGAGACCCATGTATTTc3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
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bioRxiv - Cancer Biology 2023Quote: ... and MIGR1-Cyclin E-AA (Addgene #47498, a gift from Alex Minella)67 for expressing the mutant Cyclin E ...
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bioRxiv - Immunology 2021Quote: ... Plasmid encoding EGFP-tagged PP1 catalytic subunit γ was obtained from Addgene (Addgene plasmid # 44225). Expression of PTSN was silenced in U2-OS and 293A cells by lentiviral transduction of a pLKO1 shRNA against both β- and α-PTSN (TRCN0000282572 ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were co-transfected with plasmid encoding mCherry-tagged Parkin (31) (Addgene plasmid #23956;) and encoding either ABCB-ChR2-YFP or ABCB-YFP using the Neon Transfection System (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... were transformed with a plasmid encoding the GST-tagged domain of Rhotekin (Addgene plasmid # 15247). Cells were cultivated in Terrific broth (TB ...
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bioRxiv - Cell Biology 2021Quote: ... the GST-tagged anti-GFP nanobody construct in pGEX-6P-1 vector (Addgene ID # 61838) was transformed to BL21 RIL (DE3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids for the expression of myc-tagged human ACE2 (pCEP4-myc-ACE2, Addgene No. 141185), 8his-tagged monomeric sACE2 (ACE2 a.a ...
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bioRxiv - Genomics 2022Quote: ... and cloned into a lentivirus-based sgRNA vector tagged with GFP (Addgene plasmid no. 65656). Cas9-expressing T-ALL cell lines were transduced with sgRNA library virus at a low MOI (∼0.3) ...
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bioRxiv - Microbiology 2024Quote: Triple FLAG-tagged Vpr and Vpx lentiviral expression vectors for HIV-2RODVpx (Addgene plasmid #115816), SIVSAB-92018Vpr (Addgene plasmid #115822) ...
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bioRxiv - Cell Biology 2023Quote: ... Both GFP11-tagged Fluc proteins and GEM transcriptional factor (cloned from pJW1663, Addgene plasmid #112037) were stably integrated into yeast genome ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Vector pDONR223-SYK for GST-tagged full-length SYK was purchased from Addgene (Plasmid # 23907). Vector pDONR221-FCER1G for vFlag-tagged full length FCER1G was purchased from DNASU (Clone ID HsCD00075915) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli cells containing the plasmid pCAS were obtained from Addgene (#60847). Plasmid pCAS (8.7 Kb ...
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bioRxiv - Biochemistry 2022Quote: ... coli NiCo21(DE3) cells were prepared and transformed with pMSP2N2 (Addgene) using a standard New England BioLabs (NEB ...
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bioRxiv - Immunology 2022Quote: ... coli transduced with a SpyCatcher003-mi3 expression plasmid (Addgene, Watertown, MA) were lysed with a cell disrupter in the presence of 2 mM PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were transformed with arabinose inducible EGFP-pBAD plasmid (Addgene #54762) overnight at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... coli colonies with the pET28a-EGFP-CNA35 gene (Addgene, Plasmid #61603) were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: Plasmids used in generating recombinant SA11 rotaviruses were obtained from Addgene [https://www.addgene.org/Takeshi_Kobayashi/] and included pT7/VP1SA11 ...
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bioRxiv - Cell Biology 2023Quote: ... transduced with recombinant adeno-associated virus (rAAV) (Addgene, pAAV TBG FFLuc) supernatant ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant lentivirus production was conducted according to standard protocols from Addgene using pBOB-synP-HTB in combination with psPAX2 and pCAG-VSVG as packaging and enveloping helper plasmids respectively ...
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bioRxiv - Cell Biology 2020Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol (Tropea et al. ...
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bioRxiv - Bioengineering 2019Quote: ... TGFB1-bio-His (proTGFβ) which was a gift from Gavin Wright (Addgene plasmid # 52185) [17] and HA-OVOL2 (OVOL2 ...
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bioRxiv - Cell Biology 2019Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol(Tropea et al. ...
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bioRxiv - Biochemistry 2022Quote: ... pET29b-IPP1-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124137). 15µL of Lemo21(DE3 ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-E-Syt1 was a gift from Pietro De Camilli (Addgene plasmid # 66830) (41) ...
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bioRxiv - Developmental Biology 2023Quote: ... Bot oligo – 5’-aaacTTGGCACTCCATTAGATCCG-3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Biophysics 2024Quote: ... MDCK -wildtype or -transformed cells were transfected with E-cadherin-GFP plasmid (Addgene) using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Plasmid RB-GFP FL for expression of GFP-tagged RB was obtained from Addgene (Catalog #16004). For ectopic gene expression ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 ng reference reporter (pcDNA3.1-Nanoluc-3xFLAG-V5) and 50 ng 3xFLAG tagged constructs (Addgene #87063). Transfection was carried out using Lipofectamine 3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... iEC-ESCs and iHUF cells were tagged with Azurite blue using pLV-Azurite (Addgene plasmid 36086). Lentiviral transductions were performed according to manufacturers’ protocols and successfully tagged cells were further sorted on a Beckman Coulter MoFlow Astrios (Indianapolis ...
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bioRxiv - Cell Biology 2022Quote: Human PFKFB3 tagged with N-terminal GFP was cloned into the viral plasmid pWPXL (Addgene #12257). A PFKFB3 mutant (nuc-free PFKFB3 ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... The cDNAs for eGFP-tagged SpvB(Salmonella SpvB 375-591) sequence were obtained from Addgene (#89446) and cloned into the pMSCV-puro vector.
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bioRxiv - Cancer Biology 2024Quote: ... containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene, #11770), and 4 µL of Lipofectamine 2000 reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... compact BFP-tagged CRISPRi sublibraries containing 5 sgRNAs per TSS (Addgene, Cat#83971-3 and #83975) expressed in the pCRISPRi-v2 expression vector (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminal HA-tagged full-length pCGN-ATF6-N plasmid came from Addgene (catalog #: 11974, human). The XBP1s plasmid was a kind gift from Dr ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... cells were transfected with a Myc-tagged LC3 ectopic expression plasmid (pCMV-myc-LC3; #24619, Addgene) using Lipofectamine 3000 transfection reagent (#L300015 ...
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bioRxiv - Biochemistry 2019Quote: ... coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was co-transformed with the plasmids pAC-BETAipi (Addgene), pCDFDuet-OsD27-trAtCCD7 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 transformed with the pTAT-Cre plasmid (#917, Addgene plasmid #35619) were grown for 16 hours in LB containing 100 µg/mL kanamycin ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli MG1655 strain bearing the mutagenesis machinery encoded in the pORTMAGE3 (Addgene Plasmid Number 72678 ...
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bioRxiv - Biophysics 2019Quote: ... coli K12 MG1655 genome or the pET21b-RL027A (Addgene, Cambridge, MA, USA), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... coli expression plasmid obtained as a gift from Niels Geijsen (Addgene #62731) (D’Astolfo et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Escherichia coli expression plasmids used in this study were acquired from Addgene, made possible through a gift by Cheryl Arrowsmith ...
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta(DE3) harbouring NPLOC4 and UFD1 (pAB720 and Addgene #117107, respectively) were cultured in LB as described above ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain NiCo21(DE3) (Dr Ali Ryan, Northumbria University) containing pMSP2N2 (Addgene), and cells grown aerobically at 37 °C with 225 rpm shaking to mid-exponential phase (OD600 ∼0.6) ...
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bioRxiv - Microbiology 2024Quote: ... coli K-12 MG1655 and LC-E7543 (derivative of MG1655, Addgene #115925) were used for the CRISPRi screen ...