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Citations for Addgene :
201 - 250 of 858 citations for Human Coronavirus 229E Nucleoprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: The human Dolcetto CRISPR inhibition pooled library (Addgene #92385), and the plasmids pLX_311-KRAB-dCas9 (Addgene #96918) ...
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bioRxiv - Immunology 2023Quote: The human genome-wide CRISPRa-V2 library (Addgene 1000000091) was co-transfected with packaging plasmids pCAG-VSVG and psPAX2 (Addgene plasmids 35616 and 12260 ...
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bioRxiv - Immunology 2023Quote: ... The GeCKO V2 human CRISPR knockout library from Addgene was then introduced into Endura Electrocompetent Cells by means of electroporation using a Bio-Rad Gene Pulser ...
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bioRxiv - Cancer Biology 2022Quote: ... Human STIM1-CFP plasmid (52) was from Addgene (#19755). CAV1-mEGFP plasmid (53 ...
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bioRxiv - Molecular Biology 2023Quote: The Human GeCKOv2 CRISPR knockout pooled library19 (Addgene 1000000048), which contains 6 sgRNAs for each gene and 2,000 non-targeting control sgRNAs ...
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: The human α-SYN wild-type (Addgene ID #36046) and α-SYN (wt)-141C (Addgene ID #108866 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human LATS1 expression plasmids were obtained from Addgene (41156). Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: The lentiCRISPR v2 GeCKO Human library (Addgene plasmid #52961) was amplified following Sanjana et al. ...
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bioRxiv - Zoology 2023Quote: ... or a human TLR4 expression clone (Addgene, cat. #13018). Additionally ...
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bioRxiv - Neuroscience 2023Quote: ... a human codon optimized FLAG-Cas9 cDNA (Addgene 42230) was modified by C-terminal insertion of an additional nuclear localization signal and a 6-His tag ...
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bioRxiv - Cancer Biology 2023Quote: A human kinase domain-focused gRNA library (Addgene 117725) [25] was used to assess CRISPR Cas9 screening as a tool in breast cancer PDTO to identify whether novel vulnerabilities could be detected for each PDTO ...
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bioRxiv - Microbiology 2023Quote: ... human CRISPR Brunello lentiviral pooled libraries (#73179-LV; Addgene) were added to the cell culture at an MOI of 0.25 in the presence of polybrene (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21(DE3) RIL from the pET-derived vector 14-B (a gift from S. Gradia; Addgene 48308) in LB medium individually ...
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bioRxiv - Microbiology 2022Quote: ... coli K-12 strain MG1655 that had been transformed with retron-TIR from Shigella dysenteriae NCTC2966 (Addgene #157883) or an empty vector control (pACYC184 ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180; http://n2t.net/addgene:79180; RRID:Addgene_79180) (Jarmander et al. ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS 2-6-3 were transduced with pLenti CMV rtTA3 Blast (w756-1, plasmid #26429; Addgene, gift from E. Campeau) for the expression of rtTA ...
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bioRxiv - Microbiology 2020Quote: ... or human ACE2 ectodomain (containing a C-terminal His tag) were made according to the E and F sections of the pLKO.1 Protocol from Addgene (http://www.addgene.org/protocols/plko/) ...
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bioRxiv - Immunology 2022Quote: ... against the respective genes were designed with e-crisp.org [76] and cloned into the expression vector LentiCRISPRv2 (Feng Zhang, Addgene #52961). Lentiviral particles were produced by transiently transfecting HEK 293T cells with a total of 15 µg DNA consisting of the plasmids pCMV-dr8.91 (coding for HIV gag-pol) ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA oligonucleotides were inserted into the U6>sgRNA(F+E) vector which was a gift from Lionel Christiaen (Addgene plasmid # 59986 ...
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bioRxiv - Cell Biology 2023Quote: ... Bgl2 and Mfe1 restriction enzymes were used to remove the ESYT1 sequence from the EGFP-E-Syt1 vector (Addgene, 66830) and ligate the Atp2c2c-FLAG sequence amplified from the pcDNA3.1-SPCA2C-FLAG vector ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli NiCo21 (DE3) competent cells were transformed with the plasmid encoding sumo-tag fused DiCas7-11 (Addgene: 172503). A single colony was picked and transferred to 100mL LB media containing 50 μg/mL ampicillin and grown for 12 hours at 37°C before inoculation into 1L LB culture ...
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bioRxiv - Biochemistry 2019Quote: ... pyogenes Cas9 (SpCas9) were expressed in Escherichia coli strain BL21 (DE3) using the expression plasmid pMJ806 (Addgene plasmid # 39312) (Jinek et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Precission protease was produced as a GST fusion in Escherichia coli BL21 (DE3) from pGEX-6P-1 vector (Addgene). The cleaved Fc-fusion protein were passed through a protein-A column ...
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bioRxiv - Molecular Biology 2022Quote: ... coli were constructed via an intermediate plasmid pCas9SX derived by replacing the lambda red ORF of pCasPA (Addgene #113347) (Chen et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli codon optimized TgTR was cloned into a pMAL-c2X expression vector (kind gift of Paul Riggs, Addgene #75286) containing an N-terminal maltose binding protein (MBP ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding Str-KDEL_SBP-EGFP-EGFR were generated by replacing the DNA fragment encoding E-cadherin within the plasmid Str-KDEL_SBP-EGFP-Ecadherin (Addgene, Plasmid #65286) with a DNA fragment encoding human EGFR (amino acids 31-1210).
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bioRxiv - Cell Biology 2021Quote: ... Different sgRNA sequences targeting promoter region of Smpdl3b (Table S4) were cloned into plasmid pSLQ1651-sgRNA(F+E)-sgGal4 (Addgene #100549) and then were packed into lentivirus ...
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bioRxiv - Cell Biology 2021Quote: ... and pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE (Addgene 139090, Legut et al, 2020). Oligonucleotide sequences for single guide RNAs (sgRNAs) ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-hSyn-DIO-mCherry (titer 4.56 e+14 gc/ml) was produced in an in-house viral production facility using the plasmid ordered from Addgene (#50459). The pAAV2/9-pCAG-FLEX-alpha-synuclein(A53T)-3*Myc-WPRE (mutated pαSyn ...
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bioRxiv - Neuroscience 2023Quote: An Adeno-associated plasmid pAAV-Cag-Egfp-Wpre-Sv40 (gift from Minmin Luo) was used as the backbone to generate the viral expression constructs pAAV-Cag-Apobec1-Yth-Egfp (Yth-E, Addgene #209322), pAAV-Cag-Apobec1-Ythmut-Egfp (Ythmut-E ...
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bioRxiv - Bioengineering 2023Quote: ... Murine Stem Cell Viruses (MSCV) were packaged using Platinum-E cells by co-transfection of MSCV retroviral transfer plasmids with pCL-Eco (Addgene #12371) using X-tremeGENE 9 DNA Transfection Reagent (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... VSV-G-tagged human Lrp6 was obtained from Addgene (27282), amplified by PCR using primers containing Gateway sequences ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... Human codon optimized Cas9 nuclease (hCas9) was used (Addgene (41815)) ...
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bioRxiv - Cell Biology 2019Quote: A human CRISPR KO Pooled Library (GeCKO v2) (Addgene, 1000000048) was used to introduce mutations in the H4-tfLC3B genome ...
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bioRxiv - Cell Biology 2020Quote: Human PKM2 wild-type construct was purchased from Addgene (44242) and sub-cloned to pCMV-Tag2B plasmid (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... The human TdT cDNA was cloned into CTV (Addgene #15912) construct in which the TdT expression was driven by CAG promotor and followed by a EGFP expression that mediated by an internal ribosome entry site (IRES ...
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bioRxiv - Immunology 2022Quote: ... The human TdT cDNA was cloned into CTV (Addgene #15912) construct in which the TdT expression was driven by CAG promotor and followed by a EGFP expression that mediated by an internal ribosome entry site (IRES ...
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bioRxiv - Biophysics 2019Quote: The human Sox2 and Oct4 genes were purchased from Addgene and the human c-Myc and Max genes were amplified from HeLa cell cDNA (US Biological T5595-0449) ...
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bioRxiv - Biophysics 2019Quote: ... human α-actinin 1 cDNA (Addgene; pEGFP-N1 alpha-actin1) was truncated at the actin binding domain (Asp1-Ala247) ...
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bioRxiv - Cancer Biology 2020Quote: ... the Brunello Human CRISPR knockout pooled lentiviral library (Addgene 73179) was used23 ...
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bioRxiv - Cancer Biology 2020Quote: ... the Brunello Human CRISPR knockout pooled lentiviral library (Addgene 73179) was used (33) ...
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bioRxiv - Genetics 2020Quote: ... we amplified human ACE2 (hACE2) from pcDNA3.1-ACE2 (Addgene 1786) and cloned it into a lentiviral transfer pLEX vector carrying the hygromycin resistance gene using Gibson Assembly Master Mix (NEB E2611L) ...
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bioRxiv - Neuroscience 2020Quote: pET/Calmodulin construct: Untagged human calmodulin was amplified from Addgene plasmid #47603 via PCR and cloned into an empty pET/T7 expression vector (gift from H.J ...
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bioRxiv - Cell Biology 2020Quote: Human intestinal enteroids (HIEs) were transduced with pABpuro-BluF (Addgene) plasmid DNA containing 1kb of the mouse Bmal1 promoter fused to luciferase (Bmal1-luciferase)29 ...
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bioRxiv - Cancer Biology 2022Quote: A human SLC knockout library [40] was purchased from Addgene, (Cat ...
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bioRxiv - Neuroscience 2022Quote: Human DAT cDNA was amplified from YFP-hDAT (Addgene, 90228) and pHluorin was amplified from vGlut1-pHluorin (a gift from Timothy A ...
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bioRxiv - Neuroscience 2023Quote: ... For the transfection with human Pdhk1 clone (hPdhk1; Addgene: 20564). Pre-OLs after partial differentiation for 4 days from the OPCs ...
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bioRxiv - Neuroscience 2023Quote: ... full length human TPPP (BC131506) and αSynuclein (MJFF Addgene 51486) were cloned into modified pHAT bacterial expression vectors as previously described (Fu et al ...
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bioRxiv - Cell Biology 2023Quote: ... and human p55γ were obtained from Addgene (#1407, #70458, # 70459). The plasmid of human p85α was obtained from DNASU ...