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Citations for Addgene :
1601 - 1650 of 2504 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: To generate clonal cell lines that are deficient for SURF4, a sgRNA targeting SURF4 exon 2 (SI Appendix, Table S1) was cloned into the PX459 plasmid (Addgene: 62988, a gift from Feng Zhang) as previously described (95) ...
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bioRxiv - Molecular Biology 2020Quote: ... At least two independent sets of oligonucleotide pairs for gene knockdown of human MTs and the transcription factor MTF-1 (supplementary table S4) were synthesized and cloned into the pLKO.1-TCR vector (Addgene, Cambridge, MA, USA); the same vector was also used as an empty vector control ...
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bioRxiv - Neuroscience 2023Quote: ... mice were stereotaxically injected under isoflurane anesthesia (1% v/v in oxygen, 1 L/min) with 250 nL of AAV8-hSyn-DIO-hM3d(Gq)-mCherry (Addgene, Catalog # 44361-AAV8) at a rate of 50 nL/min using a 1000 nL Hamilton syringe bilaterally in the dorsal striatum (coordinates ...
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bioRxiv - Neuroscience 2022Quote: ... Err2VP16 was generated by fusing the open reading frame for the herpes simplex virus-1 (HSV-1) VP16 (amplified from pActPL-VP16AD plasmid, Addgene plasmid #15305, Watertown, USA) activation domain to Err2 ...
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bioRxiv - Microbiology 2020Quote: ... pLenti CMV Puro DEST (w118-1) and pLenti CMV Blast DEST (706-1) were gifts from Eric Campeau & Paul Kaufman (Addgene plasmids #17398, #17452, #17451). pMD2.G and psPAX2 were gifts from Didier Trono (Addgene plasmids #12259 ...
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bioRxiv - Neuroscience 2020Quote: ... and the retrograde virus carrying Cre (AAV retrograde pmSyn1-EBFP-Cre, 7.6 × 1012 GC/ml) were purchased from Addgene (Watertown, MA).
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bioRxiv - Neuroscience 2020Quote: ... AAV2-retro-EF1a-DIO-FLPo (2.6×1013 vg/ml) was packaged by Vigene Biosciences from pAAV-EF1a-DIO-FLPo-WPRE-hGHpA (gift from Li Zhang, Addgene 87306). AAV2-fDIO-mCherry was previously described(42 ...
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bioRxiv - Neuroscience 2022Quote: ... We injected a viral cocktail of AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/ml; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) in the primary sensory (S1 ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... Injections of rAAV2/9-Ef1a-DO-DIO-tdTomato-eGFP (2.3 × 1013 gc/ml; kindly gifted from Bernardo Sabatini; AddGene plasmid #37120)34 were performed in TH- and DBH-cre animals to estimate the viral spread ...
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bioRxiv - Neuroscience 2021Quote: ... with serotype and titer (vg/ml) indicated: AAV5-EF1a-DIO-hChR2(H134R)-EYFP (Suppl. Fig. 1A; Penn Vector Core; 7.4 x 1012; Addgene plasmid #20298); AAV5-CMV-EGFP (Suppl ...
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bioRxiv - Neuroscience 2020Quote: ... while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA), All animals received three viral injections in the anterior cingulate cortex in each hemisphere as follows (skull at +5.0mm to horizontal plane) ...
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bioRxiv - Neuroscience 2020Quote: ... and the control group (n = 10) received injections of AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml; Addgene, MA, USA). The injection coordinates and volumes for the three injections made into the anterior cingulate cortex were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... the experimental group (n = 12) received injections of AAV5-CaMKIIa-hM4Di-mCherry (titer 4.4×10^12 GC/ml; Addgene, MA, USA;) and the control group (n = 10 ...
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bioRxiv - Neuroscience 2020Quote: ... Drd1Cre(FK150) mice were transduced with AAV2.hSyn.DIO.hM3Dq.mCherry (8.6 x 1012 GC/ml, Addgene viral prep #44361-AAV2, Dr. Bryan Roth)107 or AAV1.CBA.DIO.rM3Ds.mCherry.WPRE (4.3×1013 GC/ml ...
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bioRxiv - Genetics 2021Quote: ... 2 ml (1.5× 106/ml) cells plated in a well of a 6-well dish were transfected with 300 ng of Act::Cas9 (Addgene #62209) and the respective gRNA and repair templates for the 25C6 (75 ng pU6-3-25C6-gRNA1 (DGRC Cat# 1547) ...
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bioRxiv - Neuroscience 2021Quote: ... AAV8-EF1a-DIO-H2B-GFP-2A-OG (10min, 1.54 × 1013 genome units per ml, custom packaged by Vigene Biosciences, Addgene plasmid #74289) was delivered through iontophoresis into target subregions in double transgenic mice of CaMKIIα-Cre ...
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bioRxiv - Neuroscience 2022Quote: ... was injected in the NAcSh of WT mice while AAV9-Syn-Flex-GcAMP6f-eYFP (Addgene, viral titer 2.1 × 1013 vg/mL) was injected in the NAcSh of cre-dependent mice (stereotaxic coordinates from Bregma ...
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bioRxiv - Neuroscience 2022Quote: ... Selective expression of GtACR2-MuGFP-Kv2.1 in preBötC neurons that project to the 7n was obtained by injections of AAV-DIO-GtACR2-MuGFP into the preBötC and the retrograde pseudotyped AAVrg-EF1α-mCherry-IRES-Cre (titre 8x1012 GC/ml; Addgene # 55632-AVVrg) into the 7n.
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bioRxiv - Neuroscience 2022Quote: ... P1 Syngap1+/fl mice were injected with an rAAV9-packaged Cre-GFP expressing virus (pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40: Addgene #105551-AAV9: Titer = 5.66×1013 GC/ml) via the superficial temporal vein (STV ...
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bioRxiv - Systems Biology 2022Quote: ... and added undiluted to K562 cells for a final cell concentration of 3-4 x 105 cells/mL for pJT126-based effector recruitment vectors (Addgene #161926) or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... AAV8-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV-DIO-hM3Di-mCitrine, 2.3×1013vg/ml, 200 nl/hemisphere, catalog #50454-AAV8, Addgene, Watertown, MA) was delivered bilaterally into the GC of Glp1r-Cre mice at the coordinates above at a depth of -3.25mm DV ...
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bioRxiv - Neuroscience 2023Quote: ... 2014) AAV2-hSyn-DIO-hM4D(Gi)- mCherry vector (titer ≥ 5×10¹² vg/mL) was attained from AddGene (catalog number 44362-AAV2). Rats were anesthetized with 2.5% isoflurane ...
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bioRxiv - Neuroscience 2023Quote: ... Cre-dependent mCherry under the human synapsin promoter in dHPC (AAV5-hSyn-DIO-mCherry, titer: 1.1 × 1013 vg/ml, Catalog #50459-AAV5, Addgene, Watertown, USA). For optogenetic experiments ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-hSyn-DIO-mCherry (titer 4.56 e+14 gc/ml) was produced in an in-house viral production facility using the plasmid ordered from Addgene (#50459). The pAAV2/9-pCAG-FLEX-alpha-synuclein(A53T)-3*Myc-WPRE (mutated pαSyn ...
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bioRxiv - Neuroscience 2023Quote: ... into the AC and a retrograde Cre virus: pENN/AAVrg-hSyn-Cre-WPRE-hGH (1.8E+13 vg/ml, 200nl, Addgene catalog #105553) into the pStr ...
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bioRxiv - Neuroscience 2023Quote: ... We bilaterally injected Cre-dependent DREADD virus (Roth, 2016): AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4E+13 vg/ml, 350nl, Addgene catalog # 44362) or AAV5-hSyn-DIO-mCherry (2.6E+13 vg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV serotype DJ hSyn-DIO-hM4Di-mCherry (6.9 × 1012 gp/mL) was generated by the Fukushima Medical University School of Medicine Viral Vector Core facility with the corresponding plasmid (Addgene #44362).
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bioRxiv - Neuroscience 2024Quote: ... We then infused a total of 0.5uL of AAV9-syn-GCaMP7f (titer 2.3×1013 GC/ml, Addgene 104488-AAV9, Watertown, MA) at two separate locations within the craniotomy ...
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bioRxiv - Neuroscience 2024Quote: ... 200 μL of the viral cocktail AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/mL; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) was administered across four sites into the primary somatosensory (S1 ...
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bioRxiv - Neuroscience 2024Quote: ... Pulled injection pipettes were beveled and back-filled with mineral oil before being loaded with one or more of the following: AAV1-Syn-ChrimsonR-tdTomato (Chrimson, 2.10e+13 gc/mL, 250 nL, Addgene #59171-AAV1), AAV5-Syn-FLEX-rc [ChrimsonR-tdTomato] (FLEX-Chrimson ...
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bioRxiv - Immunology 2022Quote: ... target cells were derived by transfection with plasmids designed to express the SARS-CoV-2 D614 Spike protein with a c-terminus flag tag (kindly provided by Dr. Farzan, Addgene plasmid no. 156420 (Zhang et al., 2020)) ...
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bioRxiv - Microbiology 2021Quote: ... The coding sequence from amino acid 747E to 1061K was amplified by PCR from the pDONR207 SARS-CoV-2 NSP3 plasmid (Addgene, #141257, a gift from Fritz Roth[32]), including a Kozak sequence and ATG start codon in the forward primer and a TAA stop codon in the reverse primer (PLPcd_Fw ...
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bioRxiv - Synthetic Biology 2019Quote: Were-1 DNA was cloned into the pBV-Luc (Addgene) vector in order to obtain a fused riboswitch-firefly luciferase (Fluc ...
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bioRxiv - Cancer Biology 2019Quote: ... a lentiviral vector pLKO.1 (Addgene, 8453, Cambridge, MA, USA) was used ...
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bioRxiv - Neuroscience 2020Quote: ... rAAV5-hSyn-DIO-mCherry (Titer ≥ 7×1012 vg.mL−1, Addgene), AAVrg-pCAG-FLEX-tdTomato-WPRE (Titer ≥ 1×1013 vg.mL−1 ...
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pLKO.1-Tet-Neo obtained from Addgene. For inducible shRNA constructs ...
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bioRxiv - Cancer Biology 2020Quote: pLKO.1-puro (Addgene #52628, a gift from Scot Wolfe) and the modified pLKO.1-puro/GFP vector system described in (Phelan et al. ...
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bioRxiv - Plant Biology 2021Quote: ... in the level 1 vector pICH47802 (Addgene, catalog number: 48007). Level 1 pICH47811-pTCSn::nls:tGFP::tATPase (position 2 ...
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bioRxiv - Plant Biology 2021Quote: ... in the level 1 vector pICH47811 (Addgene, catalog number: 48008). To amplify IPT3 promoter (gene ID v4 ...
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bioRxiv - Microbiology 2020Quote: ... pLKO.1-puro-shNT (gift from Jacob Corn, Addgene #109012) was used as a scrambled shRNA control ...
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bioRxiv - Cell Biology 2021Quote: ... and AdEasier-1 cells (gift from Bert Vogelstein; Addgene, #16399)
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bioRxiv - Neuroscience 2021Quote: ... Animals were injected with saline-diluted AAV2/1.hSyn.GCaMP6f.WPRE.SV40 (Addgene) at a depth of 250 and 550 µm (final concentration ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by pLenti-CMV-Puro DEST vector (Addgene, w118-1). The plasmids were propagated in DH5α bacterial cells (Life Technologies) ...
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bioRxiv - Biophysics 2019Quote: ... human α-actinin 1 cDNA (Addgene; pEGFP-N1 alpha-actin1) was truncated at the actin binding domain (Asp1-Ala247) ...
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bioRxiv - Cancer Biology 2020Quote: ... pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 8453 ...
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bioRxiv - Neuroscience 2019Quote: ... and β-arrestin 1-FLAG (#14687) were obtained from Addgene.
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bioRxiv - Cancer Biology 2019Quote: ... expressed from a pLKO.1-hygro plasmid backbone (Addgene #24150). Three days after lentiviral transduction ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO.1 constructs were co-transfected with psPAX2 (Addgene 12260) and VsvG (Addgene 8454 ...
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bioRxiv - Biochemistry 2021Quote: ... Nb_EfrCD#1 was cloned into expression plasmid pBXNPHM3 (Addgene #110099) using FX cloning and expressed as described previously [42] ...
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bioRxiv - Neuroscience 2023Quote: ... AAV.1.hSyn.dio.EGFP (anterograde labelling, 140 nl, Addgene, no. 50457); retrograde.AAV.hSyn1.chI.mCherry.2A.iCre.WPRE.SV40p (retrograde labelling of LHb inputs and whole-brain clearing ...