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Citations for Addgene :
101 - 150 of 626 citations for Recombinant Mouse Acp5 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Anti-mCherry GST-tagged nanobodies (Addgene #70696, (Katoh et al, 2016)) were expressed and purified according to published protocol (Katoh et al. ...
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bioRxiv - Immunology 2023Quote: Human CD86 C-terminally tagged with enhanced GFP (pCD86-EGFP, Addgene) was sub-cloned into a modified version of the MSCV2.2 retroviral plasmid in which the IRES-GFP cassette was removed ...
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bioRxiv - Developmental Biology 2024Quote: ... The gene sequences encoding N-terminally mCherry tagged Drp1 (Addgene, 49152) were inserted between the EcoRI and PstI sites of the pCDNA vector (mCherry-Drp1) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids used were coding for HA-tagged KDM4A/JMJD2A (Addgene #24180) or for an irrelevant HA-tagged construct HA-Tsg5’ as negative control (24).
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bioRxiv - Neuroscience 2024Quote: ... USA) and CXCR7-Tango plasmid (Flag-tagged ACKR3; Plasmid #66265, Addgene) were used for mammalian expression of the eGFP and Flag-ACKR3 protein ...
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Cell Biology 2020Quote: ... eIF4B and Ded1 were tagged using the pML104 vector backbone (Addgene: 67638) while eIF4A was tagged using pML107 (Addgene ...
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bioRxiv - Neuroscience 2020Quote: A SEP-tagged version of GluA224 (gift from Roberto Malinow, RRID: Addgene_24001) was inserted under the synapsin promoter in a pCI backbone ...
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bioRxiv - Cell Biology 2019Quote: myc-tagged DBC1 (myc-DBC1) cloned in pcDNA3 was obtained from Addgene, USA ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged NHERF1 originated from Vijaya Ramesh’s laboratory (from Addgene, plasmid 11635). 16E6 point mutants were created using QuikChange primer design (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (BL21DE3) expressing GST tagged anti-GFP nanobody (Addgene plasmid #61838) (Katoh et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag-tagged DAXX DNA were amplified from plasmid Flag-Daxx/pRK5 (Addgene) and assembled into pCDH-EF1α-MCS-IRES-Puro vector (System Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-HA-tagged YTHDC1 plasmid was purchased from Addgene (plasmid # 85167).
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged TurboID (promiscuous BirA mutant, V5-Turbo-NES-pCDNA3, Addgene 107169) and C-FLAG-D20 or EGFP (pEGFP-N1-FLAG ...
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bioRxiv - Biochemistry 2023Quote: ... The Myc-tagged strains were then transformed with pRS319 (RRID:Addgene_35459, ref. (79)) to introduce a LEU3 marker for selection ...
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bioRxiv - Cancer Biology 2023Quote: ... HA-tagged ubiquitin (empty vector: pRK5-HA) was obtained from ADDGENE (17608). Control siRNA and siRNA targeting USP7 was obtained from Santa Cruz Biotechnology and added at a final concentration of 30 picomolar.
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bioRxiv - Neuroscience 2023Quote: ... all NLS tagged RFP constructs with p-EGFP-N1 (Addgene; 6085-1). To qualitatively verify Cre-dependent ArgiNLS variant expression ...
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bioRxiv - Cell Biology 2023Quote: ... we used the following emerald-green GFP (emGFP)-tagged vectors from Addgene: BAZ1A (#65371) ...
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bioRxiv - Neuroscience 2023Quote: ... a Streptavidin-binding peptide-FLAG (SFB)-tagged USP7 construct56 (Addgene plasmid #99393) was transfected into HEK293T cells via PEI transfection ...
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bioRxiv - Neuroscience 2024Quote: ... C-terminally GFP-tagged Mannosidase cDNA was obtained from Addgene (plasmid #160905) and used without additional subcloning ...
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bioRxiv - Biophysics 2020Quote: ... containing N-terminal 6-His-WT-p53 (1-303) (Addgene, 24859), was used to express p53 ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with tandem mRFP-GFP fluorescent-tagged LC3 (ptfLC3) (Addgene #21074). 24h post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... The V5-tagged human GLUT1 construct was a gift from Wolf Frommer (Addgene) 89 ...
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bioRxiv - Immunology 2019Quote: ... Expression plasmids encoding EGFP-tagged Rab5a and its mutants were purchased from Addgene, USA ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2019Quote: ... Histidine-tagged PKA catalytic subunit (a gift from Susan Taylor, Addgene plasmid #14921) and PKI3 were previously described ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged human EZHIP was cloned into the pMT-puro vector (Addgene #17923). Transfections were performed with 2 μg plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-tagged LAMP1 was a gift from Amy Palmer (Addgene plasmid # 45147). The myc-tagged WWOX plasmid was kindly provided by R ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected with plasmids expressing Flag-tagged mTOR (Plasmid #26603, Addgene) together with HA-tagged Raptor (Plasmid #8513 ...
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bioRxiv - Cancer Biology 2024Quote: ... C-terminally FLAG-tagged ASXL1 p.G646Wfs*12 and p.Y591X were obtained from Addgene. The MSCV-T2A-Puromycin vectors encoding 3xFLAG-tagged full-length ASXL1 and truncated ASXL1 were generated by PCR amplification followed by sub-cloning using Gibson assembly.
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bioRxiv - Cancer Biology 2024Quote: ... HA or myc-tagged cDNAs were from Addgene (66851, 66852, 32834, 32836, 32839) or synthesized (IDT) ...
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bioRxiv - Molecular Biology 2024Quote: A pET28b plasmid encoding C-terminal 6xHis-tagged nuclear Pif1 (Addgene plasmid #65047)64 was employed to express and purify Pif1 from E ...
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bioRxiv - Cell Biology 2023Quote: A vector expressing HA-tagged human E6AP was obtained from Addgene (Plasmid #8658), E6AP mutants were generated using site-directed mutagenesis (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged SQSTM1 and RFP-LC3 were gifts from Qing Zhong (Addgene, 28027)20 and Tamotsu Yoshimori (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Biochemistry 2019Quote: ... pMAL-his-LbCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79008 ...
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bioRxiv - Biochemistry 2021Quote: The plasmid SARS-CoV-2 3xFlag-His-nsp5CS-nsp15 (Addgene ID: 169166) was used to express 3xFlag-His-nsp5CS-nsp15 in baculovirus-infected insect cells (Supplementary Table S1 and S2) ...