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Citations for Addgene :
101 - 134 of 134 citations for Creatine Kinase M Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... ZapA-LPETG was incubated with 0.5 mM of labelled peptide GGGC-Cy5 and 10μM Sortase 7M (purified using pET30b-7M SrtA, a gift from Hidde Ploegh, Addgene plasmid #51141) in a final concentration of 50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... vector was modified to tie resistance marker gene expression directly to Cas9-Nlux expression by the P2A peptide linker to generate the plasmid pNOC-ARS-CRISPR-P2A-BlastR (Addgene: 98147). The P2A-BlastR fragment was amplified from pNOC-ARS-destiny-P2A-BlastR-GFP (Poliner et al. ...
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bioRxiv - Bioengineering 2020Quote: ... The ScFv library with the N-terminal CD8α signal peptide was fused to the synNotch-Gal4VP64 receptor backbone (Addgene plasmid #79125) in place of the CD19-specific scFv ...
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bioRxiv - Immunology 2019Quote: ... The PresentER plasmid encoding a signal peptide from Mouse Mammary Tumor Virus envelope protein followed by the SIINFEKL epitope followed by mCherry was obtained from Addgene (#102945),a kind gift of D ...
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bioRxiv - Synthetic Biology 2020Quote: The Sav library was created based on a previously described expression plasmid that contains a T7-tagged Sav gene with an N-terminal ompA signal peptide for export to the periplasm under control of the T7 promoter in a pET30b vector (Addgene #138589)34 ...
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bioRxiv - Cell Biology 2021Quote: ... the cytosolic domain of Miro1 (Miro1(ΔTM)) and the iLID binding peptide (SspB, obtained from Addgene #60415, gift from B. Kuhlman) were fused into pmCherry-C1 using EcoRI and KpnI ...
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bioRxiv - Cell Biology 2023Quote: A synthetic sequence corresponding to 2A peptide from Thoseaasigna virus capsid protein and BspTI (AflII) restriction site was first cloned to pCXLE-EGFP (Plasmid #27082, Addgene, www.addgene.org) (Okita et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The AAV capsid AAV1-X1 was built by inserting a 7-mer peptide between AAs 588-589 of the AAV1 cap gene in AAV1-Rep-Cap (Challis et al. 2019) (Addgene 112862).
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bioRxiv - Cell Biology 2019Quote: The mCherry-DNKASH construct was made from the DNKASH-TSMod construct digested by AgeI/HpaI and mCherry from a PCR on a mCherry-cSrc construct (from M. Davidson, Florida State University, Tallahassee, FL; 55002; Addgene) (fwd ...
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bioRxiv - Genetics 2021Quote: ... 16 ml of Opti-MEM was mixed with 72 μg of base editor encoding plasmid (ABE8e, Addgene #138489 or ABE8.20-m, Addgene #136300), 8 μg of pCS-GFP plasmid and 800μl Plus reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... MSCV EZH2 ΔSET-Hygro (cat# 49403) EZH2-Y641-F (cat# 80077) and pTRIPZ M)-YFP-EZH2 (cat# 82511) were procured from Addgene USA ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 TUB-mRFP cells were generated in our lab by transduction with lentiviral vectors containing tubulin m-RFP (Addgene). HEK293T cells at a 50-70% confluence were co-transfected with lentiviral packaging vectors (16.6 μg of Pax2 ...
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bioRxiv - Neuroscience 2019Quote: ... Mice received unilateral stereotactic injections of an AAV2-hsyn-DIO-hM4D(Gi)-mCherry and an AAV9-CamKII-Cre (pENN.AAV.CamKII 0.4.Cre.SV40 was a gift from James M. Wilson (Addgene viral prep # 105558-AAV9)) into the CA1 and CA3 region of the right dorsal hippocampus ...
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bioRxiv - Microbiology 2022Quote: ... pEGFP-N1-TFEB (38119) and pEGF-N1-Δ30TFEB (44445) were obtained from Addgene (a generous gift of Shawn M. Ferguson). pEGFP-2xFYVE (140047 ...
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bioRxiv - Microbiology 2023Quote: HMGB1 plasmids for cell line construction were generated in a 2nd generation pLVX-M- puro transfer plasmid (Addgene plasmid# 125839). The HMGB1 sequence was obtained from the pcDNA3.1 Flag hHMGB1 plasmid (Addgene plasmid #31609).
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bioRxiv - Genomics 2023Quote: ... We further replaced the Cas9 cassette with an nCas9/M-MLV-RT cassette from the pCMV-PE2 plasmid (Addgene, 132775). The lentiV2-pegRNA and lentiV2-ngRNA plasmids were constructed by replacing the Cas9 and Puromycin sequences in the lentiCRISPR v2 plasmid (Addgene ...
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bioRxiv - Immunology 2023Quote: ... pDONR207 SARS-CoV-2 M and pDONR207 SARS-CoV-2 ORF3a (all plasmids were gifts from Fritz Roth via Addgene, # 141273 ...
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bioRxiv - Biochemistry 2022Quote: Plasmids encoding the full-length spike proteins with native signal peptides were cloned into the background of the HDM-SARS2-Spike-delta21 plasmid (Addgene Plasmid #155130). This construct contains a 21 amino acid c-terminal deletion to promote viral expression ...
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bioRxiv - Immunology 2022Quote: The 21-amino acid C-terminally truncated spike proteins with native signal peptides were cloned in place of the HDM-SARS2-spike-delta21 gene (Addgene plasmid, 155130). This construct contains a 21-amino acid C-terminal deletion to promote viral production ...
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bioRxiv - Biochemistry 2023Quote: The 21-amino acid C-terminally truncated spike proteins with native signal peptides were cloned in place of the HDM-SARS2-spike-delta21 gene (Addgene plasmid, 155130). This construct contains a 21-amino acid C-terminal deletion to promote viral production ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid for soluble PSD-95 PDZ domain 2 expression was obtained by first replacing eGFP into pEGFP-N1 by mIRFP via BamHI and BsrGI restriction sites (gift from M Davidson, Florida State University, and X Shu, UCSF, Addgene #54620) (Yu et al. ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis strains were generated with the ORBIT method (91). ORBIT requires transformation of the starting bacterial strain (M. smegmatis mc2155) with pKM444 (Addgene #108319), a plasmid that encodes a Che9c phage RecT annealase and a Bxb1 integrase ...
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bioRxiv - Cell Biology 2022Quote: ... REEP1-mEmerald and REEP1-mCherry plasmids were generated by inserting a codon-optimized human REEP1 gblock into mEmerald-N1 (gift from M. Davidson; Addgene #53976) or mCherry-N1 backbones (Clontech) ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Rab7A and SNAP-Rab7A Q67L were made by cloning Rab7A sequence from EGFP-Rab7A and EGFP-Rab7A Q67L plasmids into pSNAP-tag (m) Vector (Addgene #101135). Soluble BFP (mTagBFP2 ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The pSAD 11.G H2B:GFP was obtained by taking advantage of the pSAD 11.G F3 expression vector (kindly provided by M. Tripodi, Cambridge University, AddGene #32634). The nucleotide sequence encoding the histone H2B-tagged GFP was designed and ordered via GeneArts (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... we overexpressed lamin A by transiently transfecting the intact cells with m-Cherry tagged plasmid DNA for lamin A which was a gift from Michael Davison (Addgene, plasmid # 55068). To surpass lamin A expression ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: We used a virus with the Gq-coupled designer receptor exclusively activated by a designer drug (DREADD) attached to the human synapsin promoter and the m-Cherry reporter (DR, AAV2 – hSyn – hM3Dq – mCherry; Addgene, Watertown, MA), as well as an empty vector control virus (Con ...
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bioRxiv - Cell Biology 2020Quote: ... Human ASK3 cDNA was also subcloned into pcDNA3 with a C-terminal tdTomato-tag (cDNA was gifted by M. Davidson, Florida State University, via Addgene: plasmid #54653) or pcDNA4/TO with an N-terminal Venus- or EGFP-FLAG-tag ...
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bioRxiv - Microbiology 2020Quote: ... carries the marker mutation A1244G and was cloned from the tdTomato-pBAD plasmid (a kind gift from Drs. M. Davidon, N. Shaner, and R. Tsien, Addgene plasmid #54856). The humanised reporter gene Renilla luciferase (GenBank AF362549 ...
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bioRxiv - Genetics 2021Quote: ... 16 ml of Opti-MEM was mixed with 72 μg of base editor encoding plasmid (ABE8e, Addgene #138489 or ABE8.20-m, Addgene #136300), 8 μg of pCS-GFP plasmid and 800μl Plus reagent ...
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bioRxiv - Plant Biology 2024Quote: ... together with a fragment containing the nucleotides encoding for the Pip1 pro-domain but lacking the signal peptide (pJK110; Supplemental Table S4) were combined in all 64 possible combinations with pICH41264 (Addgene #4799; Weber et al., 2011) in a BpiI Golden Gate reaction (Supplemental Table S4) ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNA sequences targeting the N-terminal region of the predicted small peptides were inserted into pSpCas9(BB)-2A-GFP (Addgene plasmid #48138, gift from Feng Zhang) via its BpiI cloning sites ...
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bioRxiv - Neuroscience 2022Quote: ... Three injections of AAV9-rTH-PI-Cre-SV40 (functional, titer >7×1012 vg/ml, viral prep #107788-AAV9, Addgene, US, a gift from James M. Wilson) or were made analogously to spinal injections ...