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Citations for Addgene :
1351 - 1400 of 1731 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA) (Addgene) using standard methods (42) ...
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bioRxiv - Cell Biology 2023Quote: ... We generated monoclonal U2OS cell lines expressing the following fluorescent plasmids: 1) ptfLC3B was a gift from Tamotsu Yoshimori (Addgene plasmid #21074 ...
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bioRxiv - Neuroscience 2024Quote: ... Each eye was intravitreally injected with 1 μL of AAV2-hSyn-DIO-mCherry (∼8 x 1012 GC/mL, cat. #: 50459-AAV2, Addgene) using a custom Hamilton syringe (Borghuis Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... Each eye was intravitreally injected with 1 μL of AAV2-hSyn-DIO-hM3Gq-mCherry (∼8 x 1011 GC/mL, Addgene) using a custom Hamilton syringe (Borghuis Instruments ...
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bioRxiv - Cancer Biology 2024Quote: ... and annealed and ligated in the pLKO.1 Hygro linearized backbone (a gift from Bob Weinberg, plasmid #24150 on Addgene). Stbl3 bacteria were heat-shocked to uptake ligated plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... to target NCL translation start site and the targeting sequence was cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem (1/110) (Addgene#71707)103 according to standard protocols104 ...
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bioRxiv - Cell Biology 2024Quote: ... Short hairpin RNAs (shRNAs) targeting WSB2 or BCL-2 family proteins were subcloned into the pLKO.1 puro vector (Addgene) for gene KD ...
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bioRxiv - Neuroscience 2024Quote: ... Oertner) or AAV2/1-EF1a-DIO-iChloC-2A-dsRed (5x1013 GC/ml; Addgene plasmid #70762, a gift from T. Margrie). For calcium imaging experiments ...
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bioRxiv - Plant Biology 2024Quote: ... The CDS of the prey protein (CLPS1) was cloned and inserted into the pET His6 MBP TEV LIC cloning vector (1 M) (#29656, Addgene) cloning vector to generate fusion constructs with an MBP tag ...
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bioRxiv - Molecular Biology 2024Quote: ... A guide RNA sequence was selected to target exon 1 of MTU1 and cloned into LentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid # 52961 ...
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bioRxiv - Biochemistry 2024Quote: ... A recombinant expression construct encoding the DOT1L catalytic domain (Uniprot Q8TEK3; residues 1-420) was purchased from AddGene (AddGene #36196). A recombinant expression construct encoding the Haspin kinase domain (GSG2 ...
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bioRxiv - Neuroscience 2024Quote: ... Rats were placed under isoflurane anesthesia and received unilateral or bilateral infusions of AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 (1×1013vg/mL, Addgene) to allow for Cre-dependent expression of GCaMP in the VTA (AP -5.5 mm ...
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bioRxiv - Neuroscience 2024Quote: A small craniotomy was drilled above the right Anterior Cingulate Cortex (AP: +1, ML: -0.3, DV: -0.9) and 0.2μl of AAV1-CAG-tdTomato (59462-AAV1, Addgene, 5×10¹² vg/mL) was injected at a rate of 0.05μl/min ...
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bioRxiv - Neuroscience 2024Quote: ... sequence along with the P2A sequence (2A peptide from porcine teschovirus-1 polyprotein) at the 3’ end was obtained via PCR from Addgene plasmid #129102 and fused in-frame with the mTurquoise2 DNA sequence in the same plasmid backbone as the pAAV-hSyn-mTurquoise2 plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... At P1-P2 pups were anaesthetized with Iso-fluorane and intra-cortically injected with 1 ul of a viral solution containing (AAV-EF1A-Gephyrin.FingR-GFP-CCR5TC – Addgene#125692 and AAV-CAG-tdTomato - Addgene# 59462) into layers 2/3 using a Drummond Nanojet microinjector according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... with 1 µl pAAV-mDlx-GFP-Fishell-1 (83900-AAV1), kindly shared by Dr. Gordon Fishell (Dimidschstein, Chen et al. 2016) and purchased from Addgene.
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bioRxiv - Cell Biology 2024Quote: WT (M63.1: pMG-INV 36: iCas9.302) abl pre-B cells with chromosomally integrated pMG-INV reporter and pCW-Cas9 (Addgene #50661) were described previously (29) ...
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bioRxiv - Immunology 2024Quote: ... the oligo targeti ng NAIP (5’-CCGGGCCGTGGTGAACTTTGTGAATCTCGAGATTCACAAAGTTCACC ACGGCTTTTTG-3’ and 5’-AATTCAAAAAGCCGTGGTGAACTTTGTGAATCTCGAGA TTCACAAAGTTCACCACGGC-3’) was cloned into pLKO.1 puro (8453, Addgene), w hich was then used for lentiviral construct as above ...
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bioRxiv - Developmental Biology 2024Quote: Approximately 1 × 106 trisomic proband iPS cells were transiently co-transfected with a plasmid that expressed a gRNA (Addgene: 229941) specific to the chromosome 8 centromere region and the mCherry-KNL1Mut-dCas9 plasmid ...
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bioRxiv - Developmental Biology 2024Quote: ... gRNAs 1 and 2 (Supplemental Table 2) were cloned into the pSpCas9n(BB)-2A-Puro (PX462) V2.0 plasmid (Addgene 62987) as described previously70 ...
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bioRxiv - Cancer Biology 2024Quote: ... gRNA was cloned into the Bbs1 restriction site of the pX330-U6-Chimeric_BB-CBh-hSpCAas9-hGem (1/110) vector (Addgene #71707) before transformation into NEB® 5-alpha Competent E ...
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bioRxiv - Cell Biology 2024Quote: ... 100,000 HeLa M cells were plated per well in a 6-well plate and transfected the following day at a ratio of 1:15 with the pEGFP-Puro plasmid (45561; Addgene) and gRNA-containing plasmid (pX330A-1×2) ...
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bioRxiv - Cell Biology 2024Quote: ... of ArfGAP1 was amplified from pGEX-6P-1-hs-ALPS1-ALPS2-mCherry (a gift from Philipp Niethammer (Addgene plasmid # 187114; RRID: Addgene_187114); (Shen et al. ...
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bioRxiv - Bioengineering 2024Quote: ... the kanamycin-resistance gene AphAI flanked by an I-SceI restriction site (I-SceI-AphAI fragment) was amplified using primers 5 and 6 listed in Supplementary Table 1 from pEPkan-S (Addgene plasmid #41017 ...
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bioRxiv - Cell Biology 2024Quote: PS-DKO cells were transfected with the truncated mature form of SREBP2 (2xFLAG-SREBP2, aa 1-482, Addgene plasmid #26807), which is targeted to the nucleus where it activates cholesterol biosynthesis gene programs ...
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bioRxiv - Cancer Biology 2024Quote: 3x105 parental and clonally derived Cas9-expressing OCI-AML2 or PANC-1 cells were infected with pXPR_011 (Addgene Plasmid #59702) virus as described above ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus was produced by cotransfection of a lentiviral vector (pLJM1, pLVX, pLKO.1) and the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Biochemistry 2022Quote: Human Drp1 (Uniprot ID: O00429-4) with a C-terminal StrepII tag in pET15b (Addgene plasmid #174428) was expressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: ... pLenti CMV GFP Hygro (656-4) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17446) (Campeau et al. ...
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bioRxiv - Genetics 2021Quote: The Drosophila CRISPR genome-wide knockout library was previously described and is available from Addgene (134582-4)20,55 ...
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bioRxiv - Microbiology 2022Quote: ... The HA-NFAT1(4-460)-GFP plasmid was a gift from Anjana Rao (Addgene plasmid # 11107 ;; RRID:Addgene_11107) [50] ...
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bioRxiv - Microbiology 2022Quote: ... The HA-NFAT1(4-460)-GFP plasmid was a gift from Anjana Rao (Addgene plasmid # 11107 ;; RRID:Addgene_11107) [50] ...
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bioRxiv - Neuroscience 2024Quote: ... was injected with a mixture of Cre-expressing and Cre-dependent adeno-associated viral vectors carrying the genes for ChR2 and tdTomato (AAV9.CamKII.4.Cre.SV40 and AAV9.CAG.Flex.ChR2.tdTomato, Addgene). Following a post-injection survival period of 9 weeks ...
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bioRxiv - Neuroscience 2023Quote: Viral vectors encoding the fluorescent dopamine indicator dLight1.2 (AAV5-hSyn-dLight1.2) (Addgene, titer ≥ 4×10¹² vg/mL) and calcium indicator jRCaMP1b (AAV1.Syn.NES-jRCaMP1b.WPRE.SV40 ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then transduced with lentivirus containing the plasmid pLenti_CMV_GFP_Hygro [pLenti CMV GFP Hygro (656-4) was a gift from Eric Campeau & Paul Kaufman (Addgene viral prep # 17446-LV ...
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bioRxiv - Cell Biology 2023Quote: Linear tetra-ubiquitin fused to GST (GST-4×Ub) was cloned into a pGEX-4T1 vector (RRID:Addgene_199779). After the transformation of the pGEX-4T1 vector encoding GST-4×Ub in E ...
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bioRxiv - Molecular Biology 2024Quote: ... Lenti dCAS-VP64_Blast (4) was a gift from Feng Zhang (Addgene plasmid # 61425; http://n2t.net/addgene:61425; RRID:Addgene_61425). lenti MS2-P65-HSF1_Hygro (4 ...
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bioRxiv - Molecular Biology 2024Quote: ... lenti MS2-P65-HSF1_Hygro (4) was a gift from Feng Zhang (Addgene plasmid # 61426; http://n2t.net/addgene:61426; RRID:Addgene_61426). lentiMPH v2 (20 ...
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bioRxiv - Cell Biology 2024Quote: ... a total of 3.65 μg DNA vectors including 0.47 μg pCE-hOCT3/4 (Addgene, MA, U.S.A, #41813), 0.47 μg pCE-hSK (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... 4 week-old male p62 fl/fl mice were transduced with pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8) (Addgene item #105535-AAV8) or AAV.TBG.PI.Cre.rBG (AAV8 ...
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bioRxiv - Developmental Biology 2024Quote: ... unc-4 and vab-7 homology arms were cloned into the mNG-SEC plasmid pDD268 (Addgene #132523). Primers are listed in Supplemental Table S1.
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bioRxiv - Cancer Biology 2021Quote: ... The shRNAs targeting HDAC1 (5′-CTATGGTCTCTACCGAAAA-3′) and HDAC3 (5′-GCATTGATGACCAGAGTTA-3′) were cloned into pLKO.1-TRC lentiviral vector (Addgene, #10878). For generation of lentivirus ...
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bioRxiv - Cell Biology 2020Quote: FACS EPCAM+ stromal depleted organoids at d14 were infected with lentivirus at an estimated MOI of 0.9 according to Van Lidth de Jeude et al.72 with third generation lentiviral vectors (PGK-GFP T2A Puro, SBI cat# CD550A-1; mCherry modified from pLentiCRISPRv1 (Addgene #49545) to incorporate an EF-1a-mCherry P2A Puro cassette ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed integration into attP sites was achieved by co-injection of an attB-containing vector (400 ng µl-1) and pBS130 (encoding phiC31 integrase under control of a heat shock promoter (Addgene #26290) [73]) ...
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bioRxiv - Molecular Biology 2020Quote: ... the sequence encoding the native signal peptide and ECD of GPR56 (amino acids 1-400) was subcloned from pCAG-hGPR56-IRES-GFP (from Christopher A Walsh, Addgene 52297) into pCEP4 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The first fragment was amplified using p15-Cam-F and p15-Cam-R (Table 1) from the plasmid pEVOL-pBpF (Addgene #31190), and the second fragment was obtained from the pBAD/HisB vector (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... NCBI Accession YP_009820873.1) were cloned with an N-terminal TEV protease-cleavable His6-tag using UC Berkeley Macrolab vector 2-BT (Addgene #29666). Truncations and other modified constructs were cloned by PCR mutagenesis and isothermal assembly ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... and Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (gift from Bert Vogelstein; Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: Knockdown of TPM1 was performed using the pLKO.1 plasmid lentiviral backbone (a kind gift from Bob Weinberg, Addgene plasmid #8453) either encoding an shRNA with sequence complementary to TPM1 (shTPM1 ...
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bioRxiv - Bioengineering 2021Quote: ... The nCas9(D10A) was generated by the PCR method using previously optimized Cas9 as a template (Level 1 hCas9 module, Addgene #49771). Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966 ...