Labshake search
Citations for Addgene :
51 - 100 of 157 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... or pCas9_GFP (a gift from K. Musunuru, Addgene 44719). Repair oligos were either symmetric 100 bp ssODNs with the same orientation as the gRNA sequence (APPSwe,2 ...
-
bioRxiv - Cell Biology 2024Quote: ... pLVET-HA-K-RasG12V-IRES-GFP (Addgene Plasmid #107140) was transduced using the lentiviral system ...
-
bioRxiv - Cell Biology 2022Quote: ... The HA-ubiquitin plasmid for mammalian expression was a gift from Edward Yeh (Addgene plasmid # 18712 41).
-
bioRxiv - Cell Biology 2023Quote: Linear tetra-ubiquitin fused to GST (GST-4×Ub) was cloned into a pGEX-4T1 vector (RRID:Addgene_199779). After the transformation of the pGEX-4T1 vector encoding GST-4×Ub in E ...
-
bioRxiv - Developmental Biology 2024Quote: ... HA- human HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712; Kamitani et al., 1997)
-
bioRxiv - Molecular Biology 2022Quote: ... Most of the plasmids used in Supplementary Figure 3a were previously deposited in Addgene (AcrIIC3, Addgene #85713; AcrIIC4, Addgene #113434; Acr-E2, Addgene #85677). To clone the plasmid expressing AcrIIA4 ...
-
bioRxiv - Biochemistry 2020Quote: ... cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene). Full-length EGFP fused N-terminally to a nuclear localization signal (NLS ...
-
bioRxiv - Cell Biology 2023Quote: ... pRK5-HA-Ubiquitin-WT was a gift from Ted Dawson (Addgene plasmid 17608; http://n2t.net/addgene:17608; RRID:Addgene_17608) (Lim et al. ...
-
bioRxiv - Microbiology 2023Quote: ... pcDNA3.1-HA-Ubiquitin (Ub) was a gift from Edward Yeh (Addgene plasmid #18712; http://n2t.net/addgene:18712; RRID:Addgene_18712). Full length tsa56 (OTT_0945 ...
-
bioRxiv - Neuroscience 2022Quote: ... pECFP-RacQ61L (kind gift from Dr. K. Yamada, Addgene #105292), pEGFP-N1 (Clontech) ...
-
bioRxiv - Biophysics 2023Quote: ... pEGFP-N1 was a gift from Antony K Chen (Addgene plasmid # 172281 ...
-
bioRxiv - Physiology 2024Quote: ... The latter consisted of the pLV6 backbone and a mouse per2 promoter with adjacent luciferase sequence contained in the pGL3 basic E2 vector (Addgene plasmid 48747). To ligate the Per2:luciferase reporter with pLV6 backbone we designed a restriction cloning approach shown to be efficient in large plasmids using the QuickChange Lightning Site-Directed Mutagenesis (SDM ...
-
bioRxiv - Cell Biology 2020Quote: ... Histidine-tagged ubiquitin (pCI-His-hUbi, #31815) and Ha-tagged p62 (HA-p62, #28027) plasmids were purchased from Addgene. DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen ...
-
bioRxiv - Synthetic Biology 2021Quote: ... a small ubiquitin-related modifier (SUMO) sequence known to increase soluble expression (61) derived from pDest-Sumo (Addgene #106980) (62) ...
-
bioRxiv - Genetics 2022Quote: ... cassette from Addgene plasmid #61408 was cloned into the pStart-K vector (Addgene #20346). A Ccna1 promoter region extending 5.7 kb upstream of the start codon (exon 2 ...
-
bioRxiv - Neuroscience 2021Quote: The guides were inserted into a deadCas9-KRAB-T2A-GFP lentiviral backbone containing both the guide RNA under the U6 promoter and dead-Cas9-KRAB and GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP was a gift from Charles Gersbach (Addgene plasmid #71237 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Most of the plasmids used in Supplementary Figure 3a were previously deposited in Addgene (AcrIIC3, Addgene #85713; AcrIIC4, Addgene #113434; Acr-E2, Addgene #85677). To clone the plasmid expressing AcrIIA4 ...
-
bioRxiv - Molecular Biology 2023Quote: dCas9-VPR and Cas9 experiments were performed using a published multiplex four sgRNA construct system.79 The four sgRNAs for each of the sgRNA target sites (Kcnk9 TSS, E1, and E2) were cloned into four sgRNA backbones each containing different small RNA promoters (Addgene #53186, #53187, #53188, #53189). Golden Gate cloning into pLV-GG-hUbC-dsRED (Addgene #84034 ...
-
bioRxiv - Microbiology 2024Quote: ... coli K-12 MG1655 and LC-E7543 (derivative of MG1655, Addgene #115925) were used for the CRISPRi screen ...
-
bioRxiv - Cancer Biology 2021Quote: HEK293T cells were transfected with plasmids encoding for Flag-p105 or Flag-p27 together with the plasmid HA-Ubiquitin (Addgene #18712). After 24h ...
-
bioRxiv - Genetics 2020Quote: ... The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320) as described20 ...
-
bioRxiv - Genomics 2021Quote: ... 375 ng of Prime Editor-2 enzyme plasmid (Addgene #132776) and 125 ng of pegRNA plasmid were mixed and prepared with a transfection reagent (Lipofectamine 3000 ...
-
bioRxiv - Neuroscience 2021Quote: ... a PCR-amplified ubiquitin C promoter (UBC)-blasticidin (Bla) cassette from vector pLV-TRET-hNgn1-UBC-Bla (46)(gift from Ron Weiss, Addgene plasmid, 61473) was further added to both vector assemblies resulting in reporter plasmids PB-hRHO-DsRed-WPRE-BGH-pA-UBC-Bla and PB-mCAR-EGFP-WPRE-BGH-pA-UBC-Bla ...
-
bioRxiv - Molecular Biology 2023Quote: ... Guides were inserted into a dCas9-KRAB-T2A-GFP lentiviral backbone containing both the gRNA under the U6 promoter and dCas9-KRAB-T2A-GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, a gift from Charles Gersbach, Addgene plasmid #71237) using annealed oligos and the BsmBI cloning site ...
-
bioRxiv - Biophysics 2023Quote: ... a pQE-30/pcl-ts ind+ plasmid containing a His6-small ubiquitin-like modifier (SUMO) SARS-CoV-2 nsp12 and untagged nsp7 and 8 (Addgene no. 160540) was transformed into Escherichia coli BL21 cells (Agilent) ...
-
bioRxiv - Cell Biology 2024Quote: ... consists of the backbone of the Bmal1:luc reporter vector (pLV6-Bmal-luc) and a murine Per2 promoter with adjacent luciferase sequence which is contained in the pGL3 basic E2 vector (Addgene 48747, a gift from Joseph Takahashi). The QuickChange Lightning Site-Directed Mutagenesis (SDM ...
-
bioRxiv - Biophysics 2020Quote: RNAP core enzyme was expressed from plasmid pIA900 (Addgene #104401; 45) the expression and purification was performed as outlined by Svetlov and Artsimovitch 45 with an additional size exclusion chromatography step performed using a Superose6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Microbiology 2023Quote: ... The Cas9 enzyme encoding hCas9 plasmid was from Addgene (No. 41815). Both the plasmids were co-transfected into THP-1 monocytes using the Invitrogen Neon Transfection system according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2020Quote: ... The guides were inserted into a deadCas9-KRAB-T2A-GFP lentiviral backbone containing both the guide RNA under the U6 promoter and dead-Cas9-KRAB and GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, a gift from Charles Gersbach, Addgene plasmid #71237 RRID:Addgene_71237). The guides were inserted into the backbone using annealed oligos and the BsmBI cloning site ...
-
bioRxiv - Neuroscience 2023Quote: ... The guides were inserted into a deadCas9-KRAB-T2A-GFP lentiviral backbone containing both the guide RNA under the U6 promoter and dead-Cas9-KRAB and GFP under the Ubiquitin C promoter (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, a gift from Charles Gersbach, Addgene plasmid #71237 RRID:Addgene_71237). The guides were inserted into the backbone using annealed oligos and the BsmBI cloning site ...
-
bioRxiv - Biochemistry 2024Quote: ... Plasmids for wildtype enzyme open reading frames (ORFs) were purchased from Addgene or DNASU plasmid repository ...
-
bioRxiv - Neuroscience 2024Quote: ... which is a restriction enzyme digested product of pAAV-Ef1a:DIO-EYFP (Addgene plasmid # 27056), were then ligated using T4 DNA ligase (NEB ...
-
bioRxiv - Cell Biology 2023Quote: ... we first used the restriction enzyme PmeI to linearize the plasmid MLM3613 (Addgene #42251) 85 that harbors a codon optimized Cas9 coding sequence and a T7 promoter for Cas9 mRNA in vitro synthesis ...
-
bioRxiv - Cell Biology 2024Quote: ... we used restriction enzymes BamHI and XbaI to cut pERGFP-K20 (Addgene plasmid #133861), removing most sequence downstream GFP-3xFLAG ...
-
bioRxiv - Cell Biology 2021Quote: The following plasmids were used: pcDNA3.1 MCS-BirAR118G-HA (Addgene #36047, gift from Dr. K. Roux, (22)) ...
-
bioRxiv - Biochemistry 2021Quote: Ectopic expression of mutant HA-K-RAS4B was done using the pBABE-hygro vector (Addgene plasmid #1765) (Morgenstern and Land ...
-
bioRxiv - Biophysics 2023Quote: ... pEGFP-N1 was a gift from Antony K Chen (Addgene plasmid # 172281; http://n2t.net/addgene:172281; RRID:Addgene_172281). A DNA fragment (BamHI-LYN-Halo-BsrGI ...
-
bioRxiv - Neuroscience 2020Quote: ... restriction enzymes XhoI and HpaI were used to digest the pSico vector (Addgene plasmid #11578). Digested vector RNA was then dephosphorylated using shrimp alkaline phosphatase (Roche ...
-
bioRxiv - Neuroscience 2022Quote: The Kdm6a Enzyme-Dead (Kdm6a DeM-dead) plasmid was purified and validated by Addgene (#40619) in which alanine (A ...
-
bioRxiv - Cancer Biology 2023Quote: CRISPR-mediated ATE1 activation was performed using dCAS9 enzyme (lenti dCas- VP64_Blast, Addgene plasmid #61425), transcription activators (lenti MS2-P65- HSF1_Hygro ...
-
bioRxiv - Neuroscience 2024Quote: ... digested with BglII and NotI restriction enzymes and then ligated to pJFRC-MUH (Addgene #26213). The plasmid generated (pJFRC-MUH-MipCDS; ...
-
bioRxiv - Microbiology 2022Quote: ... coli K-12 strain MG1655 that had been transformed with retron-TIR from Shigella dysenteriae NCTC2966 (Addgene #157883) or an empty vector control (pACYC184 ...
-
bioRxiv - Cell Biology 2024Quote: ... generated through In-Fusion cloning of the insert of the original pcDNA-K-GECO1 vector (Addgene plasmid #105865)(33 ...
-
bioRxiv - Genomics 2020Quote: ... sgRNA sequences were cloned into the BsmBI restriction site of plasmid MLM3636 (a gift from K. Joung, Addgene 43860). CRISPR editing was performed as described previously2 using Cas9 plasmids pSpCas9(BB)-2A-Puro (PX459 ...
-
bioRxiv - Neuroscience 2021Quote: ... We used the following viruses: AAV1-ef1a-DIO-ChR2-EYFP (gift from K. Deisseroth; Addgene viral prep 20298-AAV1); AAV9-Ef1a-DO-hChR2(H134R)-mCherry (gift from B ...
-
bioRxiv - Cell Biology 2023Quote: ... Mis18BP120-130 was cloned in pEC-K-3C-His-GST and pET His6 MBP TEV (9C Addgene plasmid #48286).
-
bioRxiv - Neuroscience 2020Quote: ... and ligated into plasmid DNA containing the Cas9 enzyme and a puromycin resistance cassette (PX459, Addgene) followed by exonuclease treatment ...
-
bioRxiv - Cancer Biology 2023Quote: ... EMD1 and EMD2 gRNA sequences were inserted via BsmBI restriction enzyme into plentiCRISPRv2_puro vector (Addgene, 52961) and applied for lentivirus preparation ...
-
bioRxiv - Genetics 2020Quote: ... targeting multiple LTR copies were cloned into lentiviral expression vector pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene 50946, deposited by K. Yusa). For LTR silencing ...
-
bioRxiv - Systems Biology 2020Quote: ... coli K-12 MG1655 Δcrp genome was done using a two-step recombination method using pSLTS plasmid (Addgene plasmid #59386). The primers used in this method are given in Supplementary Table S2 ...