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Citations for Addgene :
51 - 88 of 88 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 76 and CaVα2δ1 (p752; CaVα2δ1 was a gift from D. Lipscombe, Addgene plasmid # 26575; http://n2t.net/addgene:26575; RRID: Addgene_26575) 77 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.75 μg of psPAX2 and 0.25 μg of pMD2.g (gifts from D. Trono, Addgene #12260, #12259). At 36 hrs post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Human H-RasG12V cDNA sequence was cloned into LeGO-iV2 and LeGO-iC2 bicistronic vectors (Addgene #27344 and #27345, respectively). Retroviruses were produced by JetPEI (Polypus-Transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... we first generated OsTIR1 parental cell line (Supplementary Fig. 1G, H) by transfecting pEN396-pCAGGS-Tir1-V5-2A-PuroR (Tigre donor, #92142, Addgene) and pX330-EN1201 (spCas9nuclease with Tigre sgRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Sequences encoding N-KSR or C-KSR were subcloned into SfiI sites of pSBbi-pur-H-2Kb (# 111623, Addgene, USA) replacing an existing H-2kb fragment ...
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bioRxiv - Cell Biology 2023Quote: HEK293T-LentiX cells were co-transfected for 12 h with 10 µg psPAX2 (kind gift from Didier Trono, Addgene #12260), 2.5 µg pMD2.G (kind gift from Didier Trono ...
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bioRxiv - Microbiology 2024Quote: ... BHK-21/WI-2 culture supernatants were filtered (0.22 μm) and used to inoculate 293T cells that had been transfected 24 h previously with pMD2.G (Addgene 12259). At 24 h post-inoculation ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 30 000 cells/cm2 and transfected 24 h after with the calcium reporter pCAG mito-GCaMP5G, a generous gift from Franck Polleux (Kwon et al., 2016) (Addgene plasmid # 105009 ...
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bioRxiv - Cell Biology 2020Quote: ... and ESRG (6 μg each) along with 3 μg of pMD2.G (gift from Dr. D. Trono; RRID:Addgene_12259) was transfected into PLAT-GP packaging cells ...
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bioRxiv - Neuroscience 2021Quote: ... 2016) (Fig. 5A, Suppl. Figs. 3C,D and 8; Salk Vector Core; 1.0 x 1012; Addgene plasmid #55636); AAV1-SynP-DIO-splitTVA-EGFP-B19G (Fig ...
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bioRxiv - Microbiology 2023Quote: The construct for GRA47 complementation was created using the pUPRT::DHFR-D plasmid backbone from Addgene (Cat#58528). The DHFR cassette was eliminated through PCR amplification using primers pUPRT::GOI GIB F and pUPRT::GOI GIB R (Table S1) ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 million cells per replicate were activated and 12-20 h later electroporated with the pSpCas9(BB)-2A-GFP plasmid (pX458; Addgene 48138) expressing the Myc sgRNA using the MaxCyte STx transfection system (MaxCyte) ...
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bioRxiv - Cancer Biology 2021Quote: MEFs were seeded in 6-well plates and transfected for 6-8 h with 1 μg of plasmid 4XCLEAR-luciferase reporter (Addgene, 66800) and 0.1 ug of CMV-Renilla Luciferase (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... pUC19 was purchased from New England Biolabs and pJKR-H-araC [25] was a gift from George Church (Addgene plasmid # 62563). Plasmids pEX04 ...
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bioRxiv - Molecular Biology 2020Quote: ... These parental BV2 or BV2-Cas9 cells were transduced for 2 d with pXPR_011 lentivirus expressing eGFP (Addgene; 59702) and an sgRNA targeting eGFP at a multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2021Quote: ... All the sgRNAs were designed using Benchling Life Sciences R&D Cloud Software (https://benchling.com/) and cloned into the pSpCas9(BB)puroV2.0 vector (Addgene #62988), which expresses both Cas9 and puromycin resistance genes23,24 ...
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bioRxiv - Developmental Biology 2020Quote: ... Hammer (National Institutes of Health) (63)) and jRGECO1a (pAAV.Syn.NES-jRGECO1a.WPRE.SV40, kind gift from D. Kim & GENIE Project, Addgene plasmid #100854, (29)) ...
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bioRxiv - Bioengineering 2024Quote: ... D-REPRESS 1 to 6 plasmids were cloned following the same manner with dRfxCas13d amplification from pXR002 (Addgene #109050)24 ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected at 4-6 h with a plasmid encoding HA-Separase (pCS2+HA-hSeparase was a gift from Marc Kirschner, Addgene plasmid # 33018), Plk1TD expression was induced 8-10 h after shake off and cells were fixed at 28 h to analyze distancing or at 32 h (20 h of S phase arrest ...
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bioRxiv - Biophysics 2022Quote: ... lentivirus was generated using pcDNA/TO/GFP H-Ras V12 (gift of Yasuyuki Fujita)[43] and pLenti CMV TetR Blast (Addgene plasmid 17492), and cells were selected based on fluorescent expression.
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bioRxiv - Molecular Biology 2023Quote: ... values were converted into copy numbers by measuring against a standard curve of the AAV transfer plasmid or the Ai9 plasmid (a gift from H. Zeng Addgene plasmid # 22799). The AAV genome copy number was divided by the number of nuclei to normalize for tissue input.
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bioRxiv - Cell Biology 2024Quote: pORANGE-mEGFP-Palm1 was based on the CRISPR/Cas9 knock-in template vector pORANGE (gift from H. MacGillavry, Addgene plasmid #131471; RRID:Addgene_131471)55 ...
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bioRxiv - Neuroscience 2021Quote: ... EnvA-Rab-CVS-N2cΔG-EGFP (Fig. 5A and Suppl. Fig. 3C,D; Columbia Vector Core; 1.0 x 109; Addgene plasmid #73461); EnvA-Rab-pSADΔG-mCherry (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... 5B and Suppl. Fig. 8B-D; UNC Vector Core; 4.0 x 1012 and Penn Vector Core; 4.25 x 1012; Addgene plasmid #20298); AAV1-EF1a-DIO-hChR2(H134R)-mCherry (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Expression vectors for NFAT4 D-site variants were prepared by subcloning residues 3 – 407 of WT NFAT4 from the corresponding mammalian expression vector (Addgene #21664) into pGEX4T1 ...
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bioRxiv - Cell Biology 2022Quote: ... was a gift from Sean Munro (MRC Laboratory of Molecular Biology). The CENPB-mCh plasmid (D. Liu et al., 2010) was generated by Michael Lampson and obtained from Addgene (#45219).
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bioRxiv - Microbiology 2023Quote: ... The mouse Cul1 and Ube2l3 coding sequences were amplified from cDNA prepared from NiMOE cells and cloned into the pLenti6/V5-D-TOPO backbone (Addgene, #22945). The primer sequences used in amplifying human and mouse CUL1 and UBE2L3 coding sequences are listed in Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The sensing domain for glucose comprised residues 24–328 of the bacterial D-galactose-binding periplasmic protein (MglB) (Addgene plasmid #163115). Site saturation mutagenesis was carried out according to a previous protocol (Liu & Naismith ...
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bioRxiv - Cancer Biology 2024Quote: ... 41 using the primers listed in table S2 and cloned into the SpeI and AgeI RE sites in pLenti6/V5-D-TOPO vector (Addgene, #22945). The DIRT domain was deleted from pLenti6-3xHA-MARCHF8 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, 12259) and pTAT (kind gift of P ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, #12259) and pTAT (kind gift of P ...
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bioRxiv - Cell Biology 2024Quote: ... the Rap1-binding domain (RBD) of RALGDS (aa 793-914) was PCR amplified from pET28-MHL-His-RALGDS: N793-F914 (H. sapiens RALGDS, Addgene #25331, deposited by Cheryl Arrowsmith), cloned into a UASp N-terminal msVenus backbone using Gibson assembly ...
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bioRxiv - Neuroscience 2024Quote: ... D) or AAV2/5-CAG-dLight1.1 (left hemisphere: one mouse, right hemisphere: four mice, 111067-AAV5, Addgene, Watertown, MA, USA, Fig. 1E, F) was injected into the dorsomedial striatum (DMS ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia nos-Cas9 (Auer et al., 2020) or co-injected with pHsp70-Cas9 (400 ng μl−1) (Addgene #45945; for D. simulans transgenesis) (Gratz et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The sequence encoding the Dam-mCherry-double degron fusion was cloned and introduced into the lentiviral vector pCW57.1 (plasmid #41393; Addgene, gift from D. Root, Genewiz). A stably expressed RPE-1 megaDam cell line was obtained by antibiotic selection (puromycin).
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bioRxiv - Biophysics 2020Quote: ... Lentiviruses were produced in 293T cells by transfecting lentiviruses with the helper plasmids pMD2.G and psPAX2 (a gift from D. Trono, Addgene plasmids #12259 and #12260), following Addgene’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... we obtained the DC3000 single effector library in pENTR/SD/D-TOPO backbones and the matching Gateway destination vector pCPP5372 (Addgene, (Wei et al., 2018). Single Escherichia coli colonies were picked and grown in overnight liquid Luria-Bertani (LB ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were transfected with 750 ng of an equimolar mixture of three third-generation production plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253; pMDLg/pRRE: Addgene #12251; all gifts from D. Trono) and 750 ng of plasmid encoding the gene of interest ...