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Citations for Addgene :
51 - 100 of 103 citations for Fatty acids tall oil mixed with oleic acid sulfated potassium salts since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... potassium channel and tdTomato (separated by an IRES) was generated by subcloning myc-mKCNJ2-T2A-Tomato.pCAG plasmid (#974, Addgene plasmid #60598) into a lentiviral backbone LeGo-iT2 (#809) ...
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bioRxiv - Neuroscience 2020Quote: ... This was mixed with an AAV-CAG-Flex-tdTomato (Addgene) virus in a 50:50 mix and injected into the visual cortex ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed with packaging plasmids MD2G (1 μg, Addgene 12,259) and PSPAX2 (1 μg ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were mixed with helper plasmids pCMVR8.74 (Addgene plasmid #22036) and pCAG-Eco (Addgene plasmid #35617 ...
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bioRxiv - Neuroscience 2023Quote: ... or mixed with AAV5.hSynap.FLEX.SF-iGluSnFR.A184S (Addgene, titer 1.3 x 1013). For GCaMP expression in the DRG neurons and their axonal terminals within the DCN ...
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bioRxiv - Genetics 2024Quote: ... were mixed with 500ng pCAG-loxp-stop-loxp-ZsGreen (Addgene, 51269) plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... alone or mixed with PHP.eB-E6-S5E2-dTom-nlsdTom (Addgene #135630), each diluted to a titer of 2e12 in sterile PBS ...
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bioRxiv - Bioengineering 2023Quote: ... the guide library plasmid was mixed with psPAX2 (Addgene, catalog no. 12260) and pMD2.G (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... Primary mixed cultures were transduced with AAV1-hSyn-GCaMP6f (Addgene # 100837-AAV1) or AAV5-gfaABC1D-cyto-GCaMP6fto (Addgene # 52925-AAV5 ...
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bioRxiv - Neuroscience 2024Quote: ... were mixed with the helper plasmids pMD2.G (9 µg, Addgene #12259), and psPAX2 (1 µg ...
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bioRxiv - Molecular Biology 2020Quote: ... was mixed with 4 μg of DNA RV helper plasmid (Addgene, plasmid #12371), in 1800 μl of Opti-MEM reduced serum medium (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... and mixed with AAV1.CAG.Flex.GCaMP6s.WPRE.SV40 (Addgene, lot. no. v24793, titer 1.9 x 1013), AAV1.Syn.FLEX.jGCaMP8s.WPRE (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... mixed at a 5:1 ratio with pCMV(CAT)T7-SB100 (Addgene #34879), and co-transfected to HEK293T cells using TransIT-293 (Mirus) ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng purified oligo were mixed with 5 μg lentiCRISPR v2 (Addgene #52961) backbone ...
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bioRxiv - Molecular Biology 2020Quote: ... individual lentiviral transfer plasmids were mixed with a packaging plasmid (pMDLg/pRRE, Addgene #12251), envelope protein plasmid (pMD2.G ...
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bioRxiv - Neuroscience 2024Quote: ... 1:20 of final mix) was mixed with AAV5-hSyn-DIO-mCherry-WPRE (Addgene 50459 ...
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bioRxiv - Neuroscience 2023Quote: ... Uchida) or 120nl of mixed AAV9-Ef1a-fDIO-Cre (2.5×1013 vg/ml; Addgene) and AAV2-CAG-Flex-GCaMP6f (1:2 ...
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bioRxiv - Neuroscience 2023Quote: ... 4ug of pLenti_hSyn_FLAG_GFP or pLenti_hSyn_FLAG_PR50_GFP were mixed with 8ug of psPAX2 (Addgene Plasmid #12260) and 16ug of pMD2.G (Addgene Plasmid #12259 ...
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bioRxiv - Genetics 2023Quote: ... or the CRISPR screen plasmid library was mixed with helper plasmids psPAX2 (Addgene, 12260) and pVSV-G (Addgene ...
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bioRxiv - Genomics 2024Quote: ... 1.5 µg TF ORF lentiviral plasmid was mixed with 1.125 µg psPAX2 (#12260, Addgene), 0.375 µg pMD2.G (#12259 ...
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bioRxiv - Neuroscience 2024Quote: ... mixed at a 1:1 ratio with AAV8-Ef1a-Con/Fon-mCherry (Addgene #137132) or pAAV8-hsyn-DIO-mCherry (Addgene #50459 ...
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bioRxiv - Cell Biology 2024Quote: ... The cell pellet was mixed with 500ng of plasmid (pCS2+MLS-HyPer7, Addgene # 136470) and taken up by the Neon Transfection System pipette ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified amplicons were mixed (30 ng each) with 50 ng pJET1.2-STOP-dsRed (51) (Addgene plasmid # 60944 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:20 of final mix) was mixed with AAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene 44361 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1000k pelleted hiPSCs were mixed with 1 μL 10 μM SP-dCas9-VPR (Addgene, 63798), 9 μL Buffer R2 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... The transposon plasmid was then mixed with a plasmid encoding transposase (pCMV-SB100; Addgene Plasmid #34879) in injection buffer (2.5 ng/µl transposon plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... primers 1033F-1038F were individually mixed with primer 1039R and pLdCH plasmid DNA (Addgene #84291, (7)) to amplify an sgRNA expression cassette ...
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bioRxiv - Microbiology 2024Quote: ... iBMDM cell suspensions were mixed with 2 µg of pEGFP VAMP2 (mouse; Addgene plasmid # 42308 (51). Cells were electroporated using the program FF-100 and immediately transferred to pre-warmed DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... 10ug of each encoding plasmid was mixed with 7.5μg of ps-PAX2 and 2.5μg of PMD2.G plasmid (Addgene). Upon 10 min of RT incubation ...
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bioRxiv - Neuroscience 2021Quote: ... 6-7E6 cells were resuspended in nucleofection solution and mixed with 3ug of pCAG-EGFP plasmid (Addgene, 89684) and 600nM of siRNA ...
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bioRxiv - Biochemistry 2022Quote: ... and HTT 2088-3144 was mixed with 600 ng of the destination vector pDEST_gateway-2xFLAG (Addgene plasmid #118372), 1 μl of LR clonase ...
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bioRxiv - Neuroscience 2024Quote: ... Pipettes were first backloaded with mineral oil and then front filled with AAV1.Syn.Chronos-GFP.WPRE.bGH (Addgene, #59170-AAV1, titer: 1.4e13, 2.2e13), AAV5.Syn.Chronos-GFP.WPRE.bGH (Addgene ...
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bioRxiv - Immunology 2021Quote: ... The DNA of the constructs was mixed with the lentiviral envelope and packaging vectors pMD2-G and psPAX2c (Addgene) and polyethylenimine (PEI ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µl of the ESCs were then mixed with the scCRISPR construct (Composition: 10µl elute of HDR PCR product; 1µl sgPal7 (Addgene, 71484); 1µl spCas9-BlastR (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... 1.3×1012 vg/mL) was mixed with AAV1-CAG-FLEX-EGFP-WPRE (Addgene; Cat # 51502; ≥ 1×1013 vg/mL) in a 4:1 ratio ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV9-EF1a-DIO-ASAP6b-Kv-WPRE (1.1×10^13 vg/mL)) mixed with diluted CaMKIIα Cre virus (2.9×1013 GC/mL, Addgene #105558). All the GEVI viruses were produced by the Stanford GVVC ...
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bioRxiv - Cell Biology 2022Quote: ... 5.25 μg of transfer plasmid (LentiGuide-Hygro with 10-target or 20-target mgRNA) was mixed with 0.75 μg of pMD2.G (Addgene #12259) and 1 μg of psPAX2 (Addgene #12260) ...
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bioRxiv - Genetics 2021Quote: ... 16 ml of Opti-MEM was mixed with 72 μg of base editor encoding plasmid (ABE8e, Addgene #138489 or ABE8.20-m, Addgene #136300), 8 μg of pCS-GFP plasmid and 800μl Plus reagent ...
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bioRxiv - Synthetic Biology 2022Quote: ... Saturated cultures were centrifuged and resuspended in infiltration medium as described above except that cultures were diluted to 0.3 OD600nm and Agrobacterium strains were mixed in equal ratio with a strain containing pTRV1 (Addgene #148968). Seed of transgenic N ...
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bioRxiv - Developmental Biology 2023Quote: Transgenic animals were generated by injecting transgenes (15 ng μl-1 each) mixed with a co-injection marker pRF-4 (120 ng μl-1) (Addgene) expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells were transfected with 6 μg of pLenti6/V5-EXPR-Apaf1-mEGFP mixed with 4 μg of pCMVR8.74 (gift from Didier Trono, Addgene #22036) and 4 μg of pMD2.G (gift from Didier Trono ...
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bioRxiv - Genomics 2024Quote: ... lentivirus containing our gRNA of interest was produced from HEK-293T cells transfected with the gRNA-containing plasmid mixed with the packaging vectors pVSVG (plasmid no. 8454; Addgene) and psPAX2 (plasmid no ...
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bioRxiv - Neuroscience 2021Quote: ... was mixed 1:1 with the retrogradely trafficked AAV encoding eGFP (AAVrg-hsyn-EGFP, 7.4 × 1012 vg/mL; Addgene, Watertown, MA) and infused into the BLA (AP ...
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bioRxiv - Neuroscience 2022Quote: ... 90ul cells suspension containing 1M cells was mixed with 10 uL DNA mix: 4 ug pSpCas9(BB)-2A-Puro (PX459) plasmid (Addgene #48139), • 0.4 ug gRNA encoding plasmid (pKLV-U6gRNA(BbsI)-PGKzeo2ABFP ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μL of the annealed dsDNA was mixed with 0.5 μL of the pWS082 entry vector (which was a gift from Tom Ellis lab and Addgene plasmid # 90516) (at a concentration of 200-300 ng/uL) ...
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bioRxiv - Neuroscience 2023Quote: ... NPC-derived NGN2-neurons (mid SCZ PRS control donors of European ancestry 2607 (XY) and 553 (XY)) were transduced with the mixed-pooled gRNA vectors (Addgene 99374) at day 17 ...
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bioRxiv - Cancer Biology 2021Quote: ... or the third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) were mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Systems Biology 2021Quote: ... and pRetroX-TRE3G and pRetroX-Tet3G vectors containing the TP53 coding sequence were mixed with pMDLg/pRRE (gift from Didier Trono, Addgene plasmid #12251) and pMD2.G (gift from Didier Trono ...
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bioRxiv - Immunology 2020Quote: ... the lentiCas9-v2 lentivirus were produced from HEK-293FT cells transfected with the lentiCas9-v2 plasmid mixed at a 2:1:1 DNA ratio of the lentiviral packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Developmental Biology 2022Quote: ... the five-copy plasmid obtained above was mixed with the pPGKFLPobpA plasmid (a kind gift from Dr. Philippe Soriano – Addgene plasmid 1379357), encoding FLPo recombinase and the mix was co-electroporated into ES cell clones which had confirmed replacement of Mir483 with PGKPuroΔtk allele (Extended Data Fig ...