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Citations for Addgene :
51 - 69 of 69 citations for D Fructose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... All the sgRNAs were designed using Benchling Life Sciences R&D Cloud Software (https://benchling.com/) and cloned into the pSpCas9(BB)puroV2.0 vector (Addgene #62988), which expresses both Cas9 and puromycin resistance genes23,24 ...
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bioRxiv - Developmental Biology 2020Quote: ... Hammer (National Institutes of Health) (63)) and jRGECO1a (pAAV.Syn.NES-jRGECO1a.WPRE.SV40, kind gift from D. Kim & GENIE Project, Addgene plasmid #100854, (29)) ...
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bioRxiv - Bioengineering 2024Quote: ... D-REPRESS 1 to 6 plasmids were cloned following the same manner with dRfxCas13d amplification from pXR002 (Addgene #109050)24 ...
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bioRxiv - Neuroscience 2020Quote: Stereotaxic injection of AAV-EF1a-DIO-HChR2(H134R)-eYFP (serotype 2/1 or 2/9, U. Penn Vector Core, Philadelphia, PA, USA or Addgene, Watertown, MA, USA) was performed in 6-8 weeks old DAT-IRES-Cre or FoxP2-Cre transgenic mice ...
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bioRxiv - Neuroscience 2021Quote: ... EnvA-Rab-CVS-N2cΔG-EGFP (Fig. 5A and Suppl. Fig. 3C,D; Columbia Vector Core; 1.0 x 109; Addgene plasmid #73461); EnvA-Rab-pSADΔG-mCherry (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... 5B and Suppl. Fig. 8B-D; UNC Vector Core; 4.0 x 1012 and Penn Vector Core; 4.25 x 1012; Addgene plasmid #20298); AAV1-EF1a-DIO-hChR2(H134R)-mCherry (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Expression vectors for NFAT4 D-site variants were prepared by subcloning residues 3 – 407 of WT NFAT4 from the corresponding mammalian expression vector (Addgene #21664) into pGEX4T1 ...
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bioRxiv - Cell Biology 2022Quote: ... was a gift from Sean Munro (MRC Laboratory of Molecular Biology). The CENPB-mCh plasmid (D. Liu et al., 2010) was generated by Michael Lampson and obtained from Addgene (#45219).
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bioRxiv - Microbiology 2023Quote: ... The mouse Cul1 and Ube2l3 coding sequences were amplified from cDNA prepared from NiMOE cells and cloned into the pLenti6/V5-D-TOPO backbone (Addgene, #22945). The primer sequences used in amplifying human and mouse CUL1 and UBE2L3 coding sequences are listed in Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... The sensing domain for glucose comprised residues 24–328 of the bacterial D-galactose-binding periplasmic protein (MglB) (Addgene plasmid #163115). Site saturation mutagenesis was carried out according to a previous protocol (Liu & Naismith ...
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bioRxiv - Cancer Biology 2024Quote: ... 41 using the primers listed in table S2 and cloned into the SpeI and AgeI RE sites in pLenti6/V5-D-TOPO vector (Addgene, #22945). The DIRT domain was deleted from pLenti6-3xHA-MARCHF8 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, 12259) and pTAT (kind gift of P ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, #12259) and pTAT (kind gift of P ...
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bioRxiv - Neuroscience 2024Quote: ... D) or AAV2/5-CAG-dLight1.1 (left hemisphere: one mouse, right hemisphere: four mice, 111067-AAV5, Addgene, Watertown, MA, USA, Fig. 1E, F) was injected into the dorsomedial striatum (DMS ...
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bioRxiv - Neuroscience 2024Quote: ... sechellia nos-Cas9 (Auer et al., 2020) or co-injected with pHsp70-Cas9 (400 ng μl−1) (Addgene #45945; for D. simulans transgenesis) (Gratz et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The sequence encoding the Dam-mCherry-double degron fusion was cloned and introduced into the lentiviral vector pCW57.1 (plasmid #41393; Addgene, gift from D. Root, Genewiz). A stably expressed RPE-1 megaDam cell line was obtained by antibiotic selection (puromycin).
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bioRxiv - Biophysics 2020Quote: ... Lentiviruses were produced in 293T cells by transfecting lentiviruses with the helper plasmids pMD2.G and psPAX2 (a gift from D. Trono, Addgene plasmids #12259 and #12260), following Addgene’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... we obtained the DC3000 single effector library in pENTR/SD/D-TOPO backbones and the matching Gateway destination vector pCPP5372 (Addgene, (Wei et al., 2018). Single Escherichia coli colonies were picked and grown in overnight liquid Luria-Bertani (LB ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were transfected with 750 ng of an equimolar mixture of three third-generation production plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253; pMDLg/pRRE: Addgene #12251; all gifts from D. Trono) and 750 ng of plasmid encoding the gene of interest ...