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Citations for Addgene :
51 - 100 of 2505 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs for Decorin and E-cadherin (Gene ID: 999) were cloned into modified pHA-N1 (#86049, Addgene) and pcDNA3-neo-Strep-flag-Cterm (#102645 ...
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bioRxiv - Cancer Biology 2024Quote: The pCC_01 – hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086) was used for CRISPR screen and validation ...
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bioRxiv - Neuroscience 2024Quote: ... trans-plasmid providing viral capsid PHP.(e)B (a generous gift from Viviana Gradinaru (Addgene plasmid #103005) and cis plasmid providing hCMV/HBA_wtRIBEYE-EGFP or tdTomato ...
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bioRxiv - Microbiology 2020Quote: ... gRNAs specific for UBE4B exon 10 were designed through the E-CRISP web server (http://www.e-crisp.org/E-CRISP/) and expressed by pLentiCRISPRv2-puro (a gift from Feng Zhang; Addgene). Guide sequence function was analyzed using the Surveyor Mutation Detection Kit (Integrated DNA Technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... human CaV3.2 (a1Ha-pcDNA3 was a gift from Dr E. Perez-Reyes, Addgene #45809 (Cribbs et al. 1998), human CaV3.3 (a1Ic-HE3-pcDNA3 also from Dr E ...
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bioRxiv - Immunology 2021Quote: ... an envelope deficient HIV-1 dual reporter construct that was cloned by recombination of the pNL.luc.R-E-plasmid (NIH AIDS Reagent Program) and the fully infectious pNL4-3 mCherry luciferase plasmid (Addgene) [24 ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA oligonucleotides were inserted into the U6>sgRNA(F+E) vector which was a gift from Lionel Christiaen (Addgene plasmid # 59986 ; http://n2t.net/addgene:59986 ; RRID:Addgene_59986) 52 ...
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bioRxiv - Molecular Biology 2023Quote: Lentiviral constructs coding for TLR (#31482) and I-SceI with donor e-GFP (#31476) were purchased from Addgene. To avoid the confounding effect of NHEJ on the repair of I-SceI-induced DNA breaks ...
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bioRxiv - Cancer Biology 2023Quote: ... PGL2 E-cad (108) Ebox Mut-Luc (kindly provided Dr. Eric R. Fearon, Addgene plasmids 19291 and 19290), and 0.025 μg of pRL-TK (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... mori Gr9 (Genbank Accession EU769120.1) was cloned into a pEG BacMam vector (Addgene plasmid #160451; from E. Gouaux)29 following an N-terminal Strep-tag II30 ...
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bioRxiv - Cancer Biology 2024Quote: The pCC_01 - hU6-BsmBI-sgRNA(E + F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086; RRID:Addgene_139086) was used for CRISPR knockout ...
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bioRxiv - Cancer Biology 2024Quote: The pCC_01 - hU6-BsmBI-sgRNA(E + F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086; RRID:Addgene_139086) was used for CRISPR knockout ...
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bioRxiv - Cancer Biology 2024Quote: ... viral particles were generated by transfecting Platinum-E ecotropic packaging cell line with retroviral vectors (pLPC-puro; RRID:Addgene_19835) encoding Xenopus Rad18 using Lipofectamine® reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The adenovirus was constructed by subcloning the existing E-cadherin tension sensor into the pShuttle-CMV vector (Addgene, 16403), followed by recombination into the pAdEasy1 vector (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... to measure BLA ACh levels (Fig. 2A-E + S2.1-S2.2) or 0.5 µL of AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) to measure BLA principal cell calcium dynamics (Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... We followed the standard principles to avoid off-target effects when choosing between multiple gRNA targets for each PE and cloned into the modified U6.3>gRNA.f+e backbone (Addgene #99139) as described in 54 ...
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bioRxiv - Molecular Biology 2022Quote: sgRNAs targeting selected MYC binding motifs (E-boxes) were designed using transCRISPR and cloned into lentiCRISPR_v2 vector (Addgene #5296126) (Table 2) ...
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bioRxiv - Genomics 2024Quote: ... were combined with CROPseq-Puro-F+E plasmid (containing the sgRNAs) or dCas9-mCherry-ZIM3-KRAB plasmid (Addgene 154473) and transfected using Lipofectamine™ 3000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and pRSV-Rev (at a ratio of 4:1:1:1, Addgene#12259, #12251, #12253)41 into HEK293T cells ...
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bioRxiv - Biochemistry 2020Quote: ... and FMRP was purchased as a gene block from IDT. The human sequence (not optimized for E. coli) for FXR1P was purchased from Addgene. The genes coding for the human fragile X proteins (FMRP isoform 1 NCBI Reference Sequence ...
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bioRxiv - Immunology 2022Quote: ... against the respective genes were designed with e-crisp.org [76] and cloned into the expression vector LentiCRISPRv2 (Feng Zhang, Addgene #52961). Lentiviral particles were produced by transiently transfecting HEK 293T cells with a total of 15 µg DNA consisting of the plasmids pCMV-dr8.91 (coding for HIV gag-pol) ...
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bioRxiv - Synthetic Biology 2022Quote: ... United States (Table S1) by using E. coli Syn61Δ3(ev5) (from the laboratory of Jason W. Chin (Addgene strain #174514)) as host ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA oligonucleotides were inserted into the U6>sgRNA(F+E) vector which was a gift from Lionel Christiaen (Addgene plasmid # 59986 ...
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bioRxiv - Cell Biology 2023Quote: ... Bgl2 and Mfe1 restriction enzymes were used to remove the ESYT1 sequence from the EGFP-E-Syt1 vector (Addgene, 66830) and ligate the Atp2c2c-FLAG sequence amplified from the pcDNA3.1-SPCA2C-FLAG vector ...
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bioRxiv - Molecular Biology 2024Quote: ... N-terminally TurboID TTP (TID-TTP) and empty TurboID (TID-E) vectors were generated by cloning TID from V5-TurboID-NES_pCDNA3 (Addgene#107169) into a modified TurboID-containing pCW57.1 vector (pCW57.1-pCW 57.1-MYC-TurboID-MCS-PGK-mCherry-P2A-rtTA) ...
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bioRxiv - Molecular Biology 2020Quote: ... The two configurations that enabled efficient packaging of full-length vector genomes (designs 1 and 4) are available on Addgene (pEJS1099: Dual-sgRNA:Design 4, Addgene #159537; pEJS1096: Dual-sgRNA:Design 1, Addgene # 159538). Oligonucleotides with spacer sequences for target genes were inserted into the sgRNA cassette by ligation into the BspQI and BsmBI cloning sites.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding Str-KDEL_SBP-EGFP-EGFR were generated by replacing the DNA fragment encoding E-cadherin within the plasmid Str-KDEL_SBP-EGFP-Ecadherin (Addgene, Plasmid #65286) with a DNA fragment encoding human EGFR (amino acids 31-1210).
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bioRxiv - Cell Biology 2021Quote: ... Different sgRNA sequences targeting promoter region of Smpdl3b (Table S4) were cloned into plasmid pSLQ1651-sgRNA(F+E)-sgGal4 (Addgene #100549) and then were packed into lentivirus ...
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bioRxiv - Cell Biology 2021Quote: ... and pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE (Addgene 139090, Legut et al, 2020). Oligonucleotide sequences for single guide RNAs (sgRNAs) ...
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bioRxiv - Bioengineering 2023Quote: ... Murine Stem Cell Viruses (MSCV) were packaged using Platinum-E cells by co-transfection of MSCV retroviral transfer plasmids with pCL-Eco (Addgene #12371) using X-tremeGENE 9 DNA Transfection Reagent (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-hSyn-DIO-mCherry (titer 4.56 e+14 gc/ml) was produced in an in-house viral production facility using the plasmid ordered from Addgene (#50459). The pAAV2/9-pCAG-FLEX-alpha-synuclein(A53T)-3*Myc-WPRE (mutated pαSyn ...
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bioRxiv - Neuroscience 2023Quote: An Adeno-associated plasmid pAAV-Cag-Egfp-Wpre-Sv40 (gift from Minmin Luo) was used as the backbone to generate the viral expression constructs pAAV-Cag-Apobec1-Yth-Egfp (Yth-E, Addgene #209322), pAAV-Cag-Apobec1-Ythmut-Egfp (Ythmut-E ...
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bioRxiv - Biochemistry 2024Quote: ... was used for preparing all solutions. ME6 cells1 (containing a scarless quadruple deletion ΔselABC ΔfdhF from E. coli strain BW25113) and plasmid pSecUAG-Evol2 (Addgene #163148) were a gift from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... we analyzed the consequences of silencing SIRT1 in LNCaP cells using two independent shRNAs based on LT3GEPIR T3G-GFP-(miR-E)-PGK-Puro-IRES-rtTA3 (Addgene #111177) (Fellmann et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse Mannosidase II amino acids 1-116 was amplified from Addgene plasmid #65261 using a forward primer that contains an XbaI site and a reverse primer with an MluI site and further subcloned upstream of the mCherry open reading frame.
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) or AAV5-pCAG-FLEX-EGFP-WPRE (1.1 x 1013 gc/ml, Addgene; 1:2 dilution) was injected in VTA (from bregma ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 P.1 (Addgene, #170450), SARS-CoV-1 (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... AAV-2/1/CAG-Flex-EGFP (Addgene), pENN.AAV.hsyn.Cre.WPRE.hGH (AAV1 ...
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bioRxiv - Biophysics 2021Quote: ... ΔmutL strains were co-transformed with MutL/MutL(R-E) expression plasmid and pTARA plasmid (for T7 RNA polymerase expression, a gift from Kathleen Matthews, Addgene plasmid #31491)73 ...
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bioRxiv - Cell Biology 2020Quote: ... 5’- CGCGTCGACATGGTGAGCAAGGGCGAGGA-3’ and mCh REV: 5’-ACGCGGATCCCTTGTACAGCTCGTCCATGC-3’ and ligating it into pENTR4 no ccDB (gift from E. Campeau, Addgene plasmid #17424) plasmid (Campeau ...
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bioRxiv - Biochemistry 2022Quote: ... The open-reading frames (codon-optimized for expression in E. coli) were cloned into the expression vector p15TV-L (AddGene ID: 26093) under the T7 promoter and in-frame with the N-terminal 6xHisTag (Twist Bioscience) ...
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bioRxiv - Genetics 2023Quote: ... EPP2 and RN2 cells were retrovirally transduced with shRNAmir constructs cloned into pMSCV-miR-E-PGK-Neo-IRES-mCherry backbone (LENC; Addgene plasmid #111163), and initial infection levels were determined by flow cytometry based on mCherry expression 4 days post transduction (day 0).
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bioRxiv - Neuroscience 2024Quote: ... A 1:2 viral mixture of pENN.AAV.CamKII 0.4.Cre.SV40 (Addgene, diluted 1:100,000) and pAAV-hSyn-DIO-EGFP (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μg pCXLE-hOCT3/4-shP53 (Addgene #27077) and electroporated using the Neon System (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library set A and B (Addgene, 92379 and 92380) separately ...
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bioRxiv - Molecular Biology 2021Quote: ... For mutagenesis of the two SIMs in sov two pairs of sgRNAs (1+4 and 3+2) were cloned into pCFD4d (Addgene 83954) (Ge et al. ...
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bioRxiv - Genetics 2024Quote: ... To determine MOI and approximation of appropriate viral volume we infected day 14 iGLUTs (0, 1, 2, 4, 8, 10, 16, 32, 64 µL) with control lentivirus (pLS-SV40-mP-EGFP; AddGene #137724) and harvested for 48hrs later ...
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bioRxiv - Neuroscience 2020Quote: ... AAV1.hSyn.GCaMP6f.WPRE.SV40 (120 nl, 2×1013 vg/mL Penn Vector Core/Addgene; diluted 1:2 to 1:10 in saline) was injected either into V1 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV9-EF1a-DIO-hChR2-EYFP (1:2, Addgene viral prep # 20298- AAV9 ...
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bioRxiv - Cancer Biology 2024Quote: Truncated CPSF6 containing amino acids 1-358 (CPSF6-358) from human isoform 1 was cloned into the pCW57.1 backbone (Addgene 71782). A hemagglutinin (HA ...