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Citations for Addgene :
801 - 850 of 1548 citations for Rat Insulin Like Growth Factor 1 IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were cloned by PCR amplification of the full-length human GPCR cDNA library (hORFeome database 8.1) or the PRESTO-Tango GPCR Kit36 (Addgene Kit #1000000068). To optimize the 5-HT sensors ...
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bioRxiv - Genomics 2020Quote: The CRISPR/Cas9 plasmid (CTCF-mouse-3sgRNA-CRISPRexp-AID) was assembled using the Multiplex CRISPR/Cas9 Assembly System kit57 (a gift from Takashi Yamamoto, Addgene kit #1000000055). Oligonucleotides for three gRNA templates were synthesized ...
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bioRxiv - Genetics 2019Quote: ... Capped Cas9 mRNA was created by in vitro transcription using Thermo Fisher mMESSAGE mMACHINE™ SP6 Transcription Kit from pCS2-nls-zCas9-nls (Addgene#47929)
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bioRxiv - Cancer Biology 2019Quote: ... and Gus (which is included in the BP reaction kit) were subcloned into the pLX301 vector (from David Root, Addgene plasmid #25895) using the Gateway LR Clonase II Enzyme mix from ThermoFisher (#11791020) ...
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bioRxiv - Neuroscience 2020Quote: ... TAL effector modules were assembled and cloned into the array plasmids pFUS using the Golden Gate TALEN and TAL Effector kit (Addgene, Cambridge, USA) according to validated procedure (Cermak et al. ...
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bioRxiv - Immunology 2020Quote: ... DT40 CL18 Fam72a−/− cells were obtained by transfecting DT40 CL18 with Nucleofector™ Kit V four constructs in PX458 (Addgene, plasmid #48138) targeting Fam72a (1.5μg each ...
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bioRxiv - Synthetic Biology 2020Quote: ... Component vectors containing markers and connectors used to construct cloning vectors using this standard were a gift from John Dueber distributed through (Kit # 1000000061, Addgene, Watertown, U.S.A.). The genes or gene fragments were cloned into the storage vector pYTK001 using a Golden Gate assembly with BsmBI ...
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bioRxiv - Biochemistry 2022Quote: ... The HTT expression constructs were assembled using the BD In-Fusion PCR cloning kit in the mammalian/insect cell vector pBMDEL (Addgene plasmid #111751), an unencumbered vector created for open distribution of these reagents.
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bioRxiv - Synthetic Biology 2022Quote: ... All plasmids in this study were constructed using Golden Gate Assembly (Engler et al., 2008) and formatted with the Yeast MoClo Toolkit (Lee et al., 2015) (Addgene Kit #1000000061). ORFs encoding previously described zinc finger and clamp proteins (Bashor et al. ...
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bioRxiv - Molecular Biology 2021Quote: The bacterial expression vector for anti-GFP nanobody pGEX-6P-1 was procured from Addgene. E ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral particles were produced according to the “pLKO.1 Protocol” provided by Addgene (Cambridge, MA). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... The pAAV-mDlx-GFP-Fishell-1 was a gift from Gordon Fishell (Addgene plasmid # 83900). The plasmid was digested with PacI and XhoI ...
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bioRxiv - Neuroscience 2020Quote: The following viruses and injection volumes were used: AAV2/1-hSyn-FLEX-ChR2-eYFP (Addgene), AAV-pgk-retro-Cre (Addgene) ...
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bioRxiv - Neuroscience 2020Quote: ... 2xmCh-KIF5C was designed by subcloning 2xmCh from KIF5C(1-560)-2xmChEF(C) (Addgene #61664) in pCDNA3.1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130) was gifted by Caroline Goujon (Addgene plasmid # 145843). For gene silencing ...
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bioRxiv - Biochemistry 2021Quote: ... pRSF-1-NMT for expression of N-myristoyl transferase in bacteria was purchased from Addgene.
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bioRxiv - Neuroscience 2021Quote: ... 1×106 cells were electroporated with 1.5 µg each of pCXLE-hsk (Addgene plasmid #27078), pCXLE-hul (Addgene plasmid #27080 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were co-transfected with 1 μg of plasmid encoding Perceval HR (Addgene ID:49083) and 100 nM of DIMT1 siRNA at 50% cell confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... we also transfected the cells with a GFP expressing vector (pEGFP-N1, Addgene #6085-1). Fluorescence images were captured with a Nikon Eclipse TE2000 inverted microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: pAAV-CAG-tdTomato (titer ≥ 1×10¹³ vg/mL) was a gift from Edward Boyden (Addgene viral prep #59462-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-DIO-hChR2(H134R)-EYFP (Addgene, 1.8*10^13 gc/ml, diluted 1:10), AAV2-hSyn-hChR2(H134R)-EYFP (UNC Vector Core ...
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bioRxiv - Biophysics 2022Quote: ... pLenti CMVTRE3G Puro DEST (w811-1) was a gift from Eric Campeau (Addgene plasmid # 27565). Both plasmids harbor a puromycin- resistant gene as a marker for antibiotic selection ...
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bioRxiv - Biophysics 2022Quote: ... pLenti-CMV-Puro-DEST (w118-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17452 ...
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bioRxiv - Cell Biology 2021Quote: ... the GST-tagged anti-GFP nanobody construct in pGEX-6P-1 vector (Addgene ID # 61838) was transformed to BL21 RIL (DE3 ...
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bioRxiv - Cancer Biology 2020Quote: Two distinct shRNA for each target gene were cloned into pLKO.1 puro (Addgene #8453) according to the corresponding protocol ...
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bioRxiv - Neuroscience 2021Quote: AAV5.CaMKII.hChR2 (H134R)-mCherry (100 μL at titer ≥ 1 × 10 13 vg/mL) from Addgene was injected in 4-week-old rats intracerebrally in the right somatosensory forepaw region ...
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bioRxiv - Biochemistry 2020Quote: ... amplifying the vector from DU67831 and the Nsp3 1-109 with linker sequence from Addgene plasmid 141257 pDONR207 SARS-CoV-2 Nsp3.
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bioRxiv - Cancer Biology 2021Quote: ... they were recombined into the AgeI-EcoRI-digested pLKO.1-TRC cloning vector (Addgene #10878), using T4 DNA ligase (Thermofisher ...
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bioRxiv - Physiology 2022Quote: ... pSF-lenti or pLKO.1 together with the two helper plasmids psPAX2 (Addgene plasmid 12260) and pMD2.G (Addgene plasmid 12259 ...
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bioRxiv - Biochemistry 2019Quote: ... and then cloned into the lentivirus expression plasmid pLenti CMV Puro DEST (w118-1) (Addgene) by Gateway cloning (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... cloned in pLKO.1 puro vector and confirmed as per the protocol from addgene (Addgene Plasmid 10878 ...
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bioRxiv - Synthetic Biology 2019Quote: We modified the vector pX330A_dCas9–1 × 4 (a gift from Takashi Yamamoto, Addgene plasmid #63598) by inserting a gBlock Gene Fragment (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... The pSFFV_mNG2(11)1-10 plasmid was a gift from Bo Huang (Addgene plasmid # 82610) (Feng et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-1.5μl of Forward and Reverse primers and 50ng of lenti-sgRNA plasmid (Addgene; 52963) in a 50μl total volume reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... the reporter plasmid was first built upon the pLKO.1 - TRC cloning vector (Addgene #10878), by substituting the Puromycin resistance gene with the p53mClover2-P2A-mRFP1 or mClover2-P2A-mRFP1 (empty control ...
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bioRxiv - Cell Biology 2022Quote: ... 2011) (pCMV-VSV-G and pLKO.1 puromycin were gifts from R. Weinberg through Addgene), using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: HEK293T cells were plated 1 day before transfection with psPax2 (viral packaging plasmid; Addgene; 12260), pCMV-VSV-G (viral envelope plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2/1-DIO-CAG-GCaMP6f was purchased from Addgene (# 100839, titer: 1.4 × 1013 gc/ml). To chemogenetically activate PVNOT neurons by means of DREADD we employed AAV2/1-DIO-hSYN1-hM3Dq-mCherry (Addgene # 44361 ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV2/1 (Penn vector core) or rAAV2-retro helper (Addgene, #81070 (Tervo et al., 2016)) ...
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bioRxiv - Molecular Biology 2022Quote: ... that had been linearized with EagI and 1 μg of pBABE-puro (Addgene plasmid #1764) which contains a puromycin resistance gene ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1-hMcl-1 was a gift from Roger Davis (Addgene plasmid 25375, Cambridge, MA, USA) and is indicated in the text as WT Mcl-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... pLenti CMV Puro LUC (w168-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17477 ...
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bioRxiv - Cancer Biology 2023Quote: SUDHL-4 cell line was infected using pLKO.1-puro-GFP U6 sgRNA (Addgene #50920) containing BAX RNA guides or non-targeting RNA guide:
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bioRxiv - Evolutionary Biology 2023Quote: 1) Cas9 vector to target the C terminus of Nelfe gene: PX459 vector (Addgene 62988) was digested using BbsI-HF (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The open reading freame (ORF) of EGFP was amplified from pEGFP-C2 (#6083-1, Addgene) using upstream and downstream primers located at the BstbI and XbaI cleavage sites ...
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bioRxiv - Cell Biology 2023Quote: The expression vector of talin 1-RFP was a gift from Michael Davidson (Addgene #55139). FL TSM and HP35st TSM were gifts from Carsten Grashoff (Addgene plasmids #101170 and #101251) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1000k pelleted hiPSCs were mixed with 1 μL 10 μM SP-dCas9-VPR (Addgene, 63798), 9 μL Buffer R2 (STEMCELL Technologies ...