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Citations for Addgene :
801 - 850 of 1633 citations for Cow Upstream stimulatory factor 1 USF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or pLKO.1-Neo-CMV-tGFP-Scramble shRNA plasmid (Addgene#136035 as scramble control). Lentiviral vectors were packaged using packaging plasmid (pCMV delta R8.2 dvpr ...
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bioRxiv - Neuroscience 2024Quote: ... 1:20 of final mix) was mixed with AAV5-hSyn-DIO-mCherry-WPRE (Addgene 50459 ...
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bioRxiv - Neuroscience 2024Quote: ... we unilaterally injected 450 nL AAV9-hSyn-flex-GCaMP7s (≥ 1×10¹³ vg/mL, Addgene)107 into lAcbSh ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of the lentiviral transfer vector G1088E_pLenti-CMV-mNeonGreen-2A-HygroR (Addgene #216279), and 1 μg of one of six spike proteins tested.
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bioRxiv - Cell Biology 2024Quote: ... subsequently Dm-KHC(1-421)-SNAP-6xHis (gift from Kapitein lab, Addgene plasmid #196976) labelled with Alexa647-SNAP dye (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... For retroviral transfections 1 μg VSVG and 1.86 μg HIV gag-pol (Addgene #14887) were used ...
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bioRxiv - Microbiology 2023Quote: ... ATG16L1 shRNA (5’-GTCATCGACCTCCGGACAAAT-3’) was inserted into pLKO.1 puro (Addgene plasmid #8453) to generate pLKO.1-ATG16L1-shRNA ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) or AAV9-Ef1a-DIO-mCherry (UNC Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) and AAV1-Syn-Flex-GCaMP6s-WPRE-SV40 (Addgene 100845-AAV1 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128) or AAV9-Ef1a-DIO-mCherry (UNC Vector core ...
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1-EF1a-DIO-mKate2-PDE4D3-Cat (Boston Children’s Hospital Vector Core; Addgene 169128), and AAV2/1-hSyn-DIO-GreenDownwardcADDis (Boston Children’s Hospital Vector Core ...
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bioRxiv - Molecular Biology 2023Quote: ... we cloned the shRNA targeting TET2 into the pLKO.1-blast vector (Addgene #26655). Plasmids were generated using PCR ...
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bioRxiv - Cancer Biology 2022Quote: ECs were transfected with 1 µg of a PGC1α promoter luciferase (Addgene # Plasmid #8888) and 35 ng of pRL/TK using PEI transfection reagent (Polyethylenimine) ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids from the step 1 were cloned into pLV GG hUbC-EGFP (84034, Addgene; dsRED was replaced with EGFP from 1168 ...
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bioRxiv - Cancer Biology 2023Quote: ... sh2: 5-CCAACAACATCTTCTGCTCC-3’ were cloned into the lentiviral vector pLKO.1 (Addgene #8453)[16] ...
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bioRxiv - Genomics 2022Quote: ... gRNAs were constructed from pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP (Addgene 84254) and pSLQ1852-2 pHR ...
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bioRxiv - Neuroscience 2023Quote: ... and CamKII-Cre (pENN.AAV.CamKII 0.4.Cre.SV40, titer≥1×10¹³ vg/ml Addgene 105558-AAV9) virus was injected in S1 barrel cortex ...
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bioRxiv - Neuroscience 2023Quote: ... Ascl1 was cloned into pLenti CMV/TO Neo empty (w215-1) plasmid (Addgene #17485).
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of a virus encoding AAV5.CamKIIa.GCaMP6f (pENN.AAV.CamKII.GCaMP6f.WPRE.SV40, Addgene viral prep 100834-AAV5) virus was injected unilaterally in the PL (AP +1.9 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then transfected with 1 μg of pHrD-IRES-Fluc (Addgene plasmid #194250) and 1 ng of CMV-Rluc reporter plasmids (78 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were transiently transfected with 1 μg of each plasmid expressing GCaMP6s (Addgene #277314.1040753) (Chen et al. ...
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bioRxiv - Microbiology 2023Quote: ... plus the pscALPS-hACE2 or -cACE2 (1 µg) or the empty pscALPS_puro (Addgene, 128504) vector as a negative control ...
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bioRxiv - Developmental Biology 2023Quote: ... and hnd-1p::ZIF-1 (pRA642) expression constructs were created in pCFJ355 (Addgene #34870), a vector designed for Mos1-mediated single copy transgene insertion on LGX (Frokjaer-Jensen et al ...
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bioRxiv - Neuroscience 2023Quote: ... 0.75 µL of rgAAV-FLEX-tdTomato (diluted 1:3 in dPBS; Addgene number: 28306) was injected in TH-Cre mice in basal forebrain (AP ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μg of target shRNA construct and 2μg of 3:1 ratio of psPAX2 (Addgene) and pMD2.G (Addgene ...
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bioRxiv - Plant Biology 2024Quote: ... for Level 1 and 3 and pEven1-4 (pCsA-E, Addgene plasmids # 136067-136070) for Level 2 ...
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bioRxiv - Biophysics 2024Quote: ... A plasmid encoding aa 1-261 of human utrophin (PaGFP-UtrCH, Addgene plasmid #26738) was obtained as a gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... human TDP-43M337V has subcloned into the pLenti CMV Puro DEST (W118-1, Addgene) plasmid as previously described (Zhang et al ...
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bioRxiv - Genomics 2020Quote: The CRISPR/Cas9 plasmid (CTCF-mouse-3sgRNA-CRISPRexp-AID) was assembled using the Multiplex CRISPR/Cas9 Assembly System kit57 (a gift from Takashi Yamamoto, Addgene kit #1000000055). Oligonucleotides for three gRNA templates were synthesized ...
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bioRxiv - Genetics 2019Quote: ... Capped Cas9 mRNA was created by in vitro transcription using Thermo Fisher mMESSAGE mMACHINE™ SP6 Transcription Kit from pCS2-nls-zCas9-nls (Addgene#47929)
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bioRxiv - Cancer Biology 2019Quote: ... and Gus (which is included in the BP reaction kit) were subcloned into the pLX301 vector (from David Root, Addgene plasmid #25895) using the Gateway LR Clonase II Enzyme mix from ThermoFisher (#11791020) ...
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bioRxiv - Neuroscience 2020Quote: ... TAL effector modules were assembled and cloned into the array plasmids pFUS using the Golden Gate TALEN and TAL Effector kit (Addgene, Cambridge, USA) according to validated procedure (Cermak et al. ...
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bioRxiv - Immunology 2020Quote: ... DT40 CL18 Fam72a−/− cells were obtained by transfecting DT40 CL18 with Nucleofector™ Kit V four constructs in PX458 (Addgene, plasmid #48138) targeting Fam72a (1.5μg each ...
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bioRxiv - Synthetic Biology 2020Quote: ... Component vectors containing markers and connectors used to construct cloning vectors using this standard were a gift from John Dueber distributed through (Kit # 1000000061, Addgene, Watertown, U.S.A.). The genes or gene fragments were cloned into the storage vector pYTK001 using a Golden Gate assembly with BsmBI ...
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bioRxiv - Biochemistry 2022Quote: ... The HTT expression constructs were assembled using the BD In-Fusion PCR cloning kit in the mammalian/insect cell vector pBMDEL (Addgene plasmid #111751), an unencumbered vector created for open distribution of these reagents.
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bioRxiv - Synthetic Biology 2022Quote: ... All plasmids in this study were constructed using Golden Gate Assembly (Engler et al., 2008) and formatted with the Yeast MoClo Toolkit (Lee et al., 2015) (Addgene Kit #1000000061). ORFs encoding previously described zinc finger and clamp proteins (Bashor et al. ...
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bioRxiv - Molecular Biology 2021Quote: The bacterial expression vector for anti-GFP nanobody pGEX-6P-1 was procured from Addgene. E ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral particles were produced according to the “pLKO.1 Protocol” provided by Addgene (Cambridge, MA). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... The pAAV-mDlx-GFP-Fishell-1 was a gift from Gordon Fishell (Addgene plasmid # 83900). The plasmid was digested with PacI and XhoI ...
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bioRxiv - Neuroscience 2020Quote: The following viruses and injection volumes were used: AAV2/1-hSyn-FLEX-ChR2-eYFP (Addgene), AAV-pgk-retro-Cre (Addgene) ...
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bioRxiv - Neuroscience 2020Quote: ... 2xmCh-KIF5C was designed by subcloning 2xmCh from KIF5C(1-560)-2xmChEF(C) (Addgene #61664) in pCDNA3.1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130) was gifted by Caroline Goujon (Addgene plasmid # 145843). For gene silencing ...
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bioRxiv - Biochemistry 2021Quote: ... pRSF-1-NMT for expression of N-myristoyl transferase in bacteria was purchased from Addgene.
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bioRxiv - Neuroscience 2021Quote: ... 1×106 cells were electroporated with 1.5 µg each of pCXLE-hsk (Addgene plasmid #27078), pCXLE-hul (Addgene plasmid #27080 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were co-transfected with 1 μg of plasmid encoding Perceval HR (Addgene ID:49083) and 100 nM of DIMT1 siRNA at 50% cell confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... we also transfected the cells with a GFP expressing vector (pEGFP-N1, Addgene #6085-1). Fluorescence images were captured with a Nikon Eclipse TE2000 inverted microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors encoding ChR2(H134R)-eYFP (titer = 1 x 1012 vg/ml, Addgene 26973, RRID:Addgene_127090) were stereotaxically injected into and centered at the temporal association cortex (TeA ...
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bioRxiv - Neuroscience 2022Quote: pAAV-CAG-tdTomato (titer ≥ 1×10¹³ vg/mL) was a gift from Edward Boyden (Addgene viral prep #59462-AAV9 ...
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bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-DIO-hChR2(H134R)-EYFP (Addgene, 1.8*10^13 gc/ml, diluted 1:10), AAV2-hSyn-hChR2(H134R)-EYFP (UNC Vector Core ...