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Citations for Addgene :
751 - 800 of 1005 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplified products were cloned into BsmB1-digested LRG2.1 (a lentiviral sgRNA expression vector, U6-sgRNA-EFS-GFP, Addgene: 108098) using the Gibson Assembly kit (NEB#E2611) ...
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bioRxiv - Cancer Biology 2019Quote: ... CDKN1A and NRF2 PCR amplicons were prepared with SpeI and MfeI restriction sites and cloned into pEN_TTmiRc2 BirA* (Addgene #136521). Luciferase PCR amplicon was prepared with SpeI and EcoRI restriction sites and cloned into pEN_TTmiRc2 3xFLAG digested with SpeI and MfeI sites (Addgene #136519) ...
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bioRxiv - Cancer Biology 2019Quote: ... and p53(R280K)-V5 PCR amplicon was prepared with SpeI and MfeI restriction sites and cloned into pEN_TTmiRc2 (Addgene #25752) digested with SpeI and MfeI (Addgene #136520 ...
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bioRxiv - Neuroscience 2020Quote: ... BirA-HA and HaloTag constructs were PCR-amplified from pcDNA3.1-MCS-BirA(R118G)-HA (a gift from Kyle Roux, Addgene #36047) (Roux et al. ...
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bioRxiv - Cell Biology 2021Quote: ... followed by digestion of the PCR product with EcoRI and SacII and ligation in between Smoothened and mCherry in mCherry-Smo (Addgene plasmid 55134 ...
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bioRxiv - Cell Biology 2021Quote: GCaMP6m was generated by PCR amplifying the GCaMP6m sequence from pGP-CMV-GCaMP6m (Addgene plasmid #40754, (Chen et al., 2013)) and ligating it into the BamH1 and EcoR1 sites in pCS2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Viral vectors were generated from Addgene plasmids #20297 and #50459 and titers determined via fluorometric quantification (Neuroscience Center Zurich) or by real-time quantitative PCR combined with SYBR green technology (Addgene). Respective titers were >9.1×1012 vector genomes/ml (Neuroscience Center Zurich ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The CyPet gene was amplified by PCR from the pCyPet-His vector (pCyPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14030 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The YPet gene was amplified by PCR from the pYPet-His vector (pYPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14031 ...
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bioRxiv - Neuroscience 2020Quote: ... and jRGECO1a(Dana et al. 2016) were first amplified by PCR from pGP-CMV-GCaMP6f and pGP-CMV-NES-jRGECO1a (Addgene) with 5’ BamHI and 3’ HindIII ...
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bioRxiv - Microbiology 2020Quote: PCR fragments corresponding to 231 nts of the Citrine coding sequence were amplified from ES-FUCCI plasmid (Addgene plasmid#62451) using primers containing T7 promoter sequence with 2 distinct PCR fragment allowing the positive-sense or negative-sense RNA ...
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bioRxiv - Neuroscience 2020Quote: ... The loxP-NeoR-loxP cassette was inserted at the MluI site in the intron between exons 1 and 2 following PCR amplification from pL452 (Addgene) using primers 3-For and 3-Rev ...
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bioRxiv - Molecular Biology 2022Quote: ... using the overlapping PCR fragment method described in (Friedland et al. 2013) or were cloned into pDD162 (Addgene plasmid #47549) using the Q5 Site Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... the p300 HAT core domain (p300 core) was PCR amplified from the pcDNA-dCas9-p300-Core vector (Addgene, Plasmid #61357) and cloned into MluI/BstXI digested pHR-TRE3G-KRAB-dCas9-P2A-mCherry backbone ...
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bioRxiv - Molecular Biology 2019Quote: ... the KRAB sequence was PCR amplified from the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2A-Puro vector (Addgene, plasmid #71236) and cloned into the enCRISPRa sgRNA vector to replace VP64 ...
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bioRxiv - Cell Biology 2019Quote: ... IRES DNA and mCherry cDNA were prepared by PCR using pEYFP SUMO-1 plasmid (from Mary Dasso: Addgene plasmid #13380), pWPI plasmid (from Didier Trono ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmids expressing dCas9-VPR were constructed by Isothermal assembly34 combining NotI linearized pMBO2744 attP vector backbone with dCas9-VPR PCR amplified from pAct:dCas9-VPR (Addgene #78898)35 and SV40 terminator for pH-Stinger (BDSC ...
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bioRxiv - Cell Biology 2020Quote: ... we amplified two DNA fragments with PCR and used Gibson assembly to subclone these into the BamHI site of pBluescript II KS+ (Addgene). The first fragment ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293Ts were made to stably express doxycycline(dox)-inducible wild-type muSOX and its catalytically-dead D219A mutant by PCR amplifying the aforementioned coding sequences from Addgene plasmids 131702 and 131704 using the muSOX F/R primers (see Key Resources Table ...
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bioRxiv - Synthetic Biology 2020Quote: ... moonbody (S5.1) cDNA was amplified via PCR and then inserted into the pSH-EFIRES-P-AtAFB2-mCherry vector (Addgene, #129716) between EcoRI and NotI sites to replace mCherry.
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bioRxiv - Cell Biology 2021Quote: ... pRS315-NOP1pro-GFP1-10-mCherry-PUS1 and pRS315-NOP1pro-GFP1-10-mCherry-SCS2TM were generated using PCR products amplified from Addgene plasmids #86413 and #86419 and Addgene plasmids #86416 and #86419 ...
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bioRxiv - Molecular Biology 2020Quote: ... the genes of NIR-GECO(1,2,2G)-T2A-GFP and were constructed using overlap-extension PCR followed by subcloning into pAAV-CAG vector (Addgene no. 108420) using BamHI and EcoRI sites ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of Cre was prepared by PCR and transferred to retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
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bioRxiv - Synthetic Biology 2020Quote: ... pHCKan-yibDp-CBD-hGLY was constructed by DNA assembly with 2 PCR products amplified from (i) a plasmid coding hGLY under a T7 promoter (pHCKan-T7-CBD-hGLY, Addgene #134940 ...
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bioRxiv - Genetics 2020Quote: ... of homology arms (∼400bp) PCR amplified from genomic DNA and the FKBP12F36V sequence amplified from plasmid pLEX_305-N-dTAG (Addgene # 91797) (Nabet et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The constitutively active form of kinesin-1 (human KIF5B, K560) was cloned by PCR from pet17-K560-GFP_His (Addgene, 15219) into pEGFP-N1 vector using EcoRI primer (104-5p EcoRI-K560 ...
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bioRxiv - Cell Biology 2021Quote: ... EGFP-Miro2 and mCherry-Miro1 were generated by PCR amplification of the coding sequence of Miro and ligated into pEGFP-C1(Addgene) or pmCherry-C1(Addgene) ...
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bioRxiv - Biochemistry 2021Quote: ... the vector backbone was PCR-amplified and the resulting fragment was ligated with the ccdB kill-cassette excised from pINIT_cat (Addgene #46858) with SapI ...
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bioRxiv - Cell Biology 2021Quote: ... T7 promoter was added to the Cas9 coding region and sgRNA-scaffold by PCR amplification of plasmid PX330 (Addgene, #42230). PCR products of Cas9 and sgRNA region were used for IVT by following the manufactural of mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: ... A cpGFP cassette was amplified by PCR from a pCDNA3.1 plasmid encoding ASAP1 (a gift from Dr. Michael Lin, Stanford, available as Addgene #52519) and inserted into pCDNA3.1-GPR68 using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: The lentiviral dCas9 expression plasmid (lenti-dCas9-Blast) was generated by PCR-based mutagenesis of lentiCas9-Blast plasmid (Addgene #52962). Clover fused with PUF RNA-binding domain were previously described ...
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bioRxiv - Cell Biology 2021Quote: The EB1-GFP lentivirus expression construct was made by PCR amplification of EB1-GFP from pEGFP N1 EB1-GFP (JB131) (gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39299 ...
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bioRxiv - Neuroscience 2020Quote: All constructs containing the N/Q-rich region of the neuronal-specific Aplysia CPEB (residues 1-160) were cloned by PCR using a full-length clone (Addgene) as the template ...
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bioRxiv - Molecular Biology 2021Quote: ... and its RdRp domain (aa 91-610) were obtained using PCR on a SINV cDNA plasmid template (pSIN ReP5-GFP-GluR1 from Addgene). The PCR fragments were subcloned into the pSUMO-LIC vector using a ligation-independent cloning method in which the expressed recombinant protein had an N-terminal hexahistidine-linked small ubiquitin-like modifier protein (His6-SUMO ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with two gRNA transcriptional cassettes prepared by PCR and CAG-Cas9-T2A-EGFP plasmid (Addgene #7831) as described elsewhere56 ...
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bioRxiv - Biophysics 2022Quote: ... and 32C (residues 172–270 of RPA2) domains of human RPA were PCR amplified using p11d-tRPA plasmid (Addgene plasmid102613) (Henricksen et al. ...
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bioRxiv - Bioengineering 2022Quote: ... After digestion with EcoRI and AgeI the PCR products were ligated into the pLVX-TetOne-Puro vector (Addgene plasmid #124797).
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bioRxiv - Cell Biology 2022Quote: The mat-Gal4-sqhUTR plasmid was generated by PCR amplification of the Gal4 coding sequence from pPac-Pl-mCD8-D2A-Gal4 (a gift from Benjamin White, Addgene plasmid # 39457 ...
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bioRxiv - Physiology 2022Quote: ... an mCherry-T2A-hM4Dinrxn cassette was PCR-amplified using CAG::mCherry-2a-hM4Dnrxn (Addgene #52523, donated by Dr. Scott Sternson) as a template with primers containing 5’-BamHI site and 3’-MluI site and then inserted into pENTR1A-TRE ...
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bioRxiv - Physiology 2022Quote: ... an hM3Dq-mCherry cassette was PCR-amplified using pAAV-hSyn-DIO-hM3Dq-mCherry (Addgene #44361, donated by Dr. Bryan Roth) as a template with primers containing 5’-MluI site and 3’-EcoRI site and then inserted into pENTR1A-TRE between the MluI/EcoRI sites ...
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bioRxiv - Plant Biology 2023Quote: ... BsaI recognition site and compatible vector overhang was used to generate specific gRNA amplicons by PCR from an gRNA scaffold template (AddGene no ...
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bioRxiv - Genomics 2022Quote: The PCR product was cloned into the pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5 vector (Addgene #6125554) using HindIII and XbaI restriction sites ...
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bioRxiv - Neuroscience 2022Quote: Full length human TAOK1 was obtained from Transomics Technologies in PCR-XL-Topo plasmid and inserted into sfGFP-C1 (Plasmid #54579, Addgene), using the EcoRI and MfeI sites ...
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bioRxiv - Molecular Biology 2022Quote: ... R179A and R320A were PCR amplified and cloned into pET Strep II co-transformation cloning vector (13S-R, Addgene # 48328). Csb3/I-G was cloned into pQE2 (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs encoding cyclin D1 WT and cyclin D1 (T286A) were PCR amplified from pcDNA cyclinD1 HA and pcDNA cyclinD1 HA T286A (Addgene plasmids #11181 and # 11182 ...
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bioRxiv - Microbiology 2022Quote: UAS-SARS-CoV-2-ORF3a transgenic flies were generated by PCR-mediated subcloning of the SARS-CoV-2-ORF3a coding sequence (pDONR207-SARS-CoV-2 ORF3a, #141271, Addgene) into pUASt-Attb (EcoRI/XbaI) ...
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bioRxiv - Microbiology 2023Quote: The construct pFUS ΔIDR-YFP was created by PCR amplifying the fus gene from pGST- TEV-FUS (a kind gift from Dr. Aaron Gitler; Addgene plasmid # 29629 ...
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bioRxiv - Neuroscience 2023Quote: ... sfGFP genes were amplified by PCR from pJT119b plasmid which was kindly gifted by by Jeffrey Tabor (Addgene plasmid #50551), and cloned into pETcon-alpha syn ...
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bioRxiv - Cell Biology 2023Quote: ... Obtained pcDNA-Halo was further digested with HindIII/Bam-HI restriction enzymes and ligated with the NPM insert (obtained by PCR amplification from eGFP-NPM (17578 Addgene) with our primers 5’-CCCAAGCTTCCACCATGGAAGATTCGATGGACATGG-3’ and 5’-CGGGATCCAAGAGACTTCCTCCACTGCC-3’ ...
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bioRxiv - Bioengineering 2023Quote: ... Human codon optimized DisCas7-11 protein sequence and the mature DR guide scaffold with golden gate sites were PCR amplified from Addgene plasmids # 172507 and #172508 ...