Labshake search
Citations for Addgene :
751 - 800 of 960 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The coding region of the SunTag and Kif18b was obtained by polymerase chain reaction (PCR) of a pCMV-SunTag-Kif18b-PP7 template (Addgene #128606), using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... the coding region of the SunTag and Kif18b was obtained by PCR of a pCMV-SunTag-Kif18b-PP7 template (Addgene #128606), using the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... The ORF was amplified by PCR and inserted into the BmtI/BspEI site of the pRSETB-mCherry vector (Addgene plasmid 32382). The construct was transformed into the E.coli strain BL21(DE3) ...
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bioRxiv - Cell Biology 2019Quote: ... OsTIR1 targeting plasmid was modified by eliminating the miRFP670 sequence by PCR amplification of left homology arm and TIR/BSD/right homology arm for inserting into pMK292 obtained from Addgene (#72830) (Natsume et al. ...
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bioRxiv - Cell Biology 2019Quote: ... mCerulean CDS was amplified by PCR using mCerulean-N1 vector as the template (Koushik et al., 2006)(a gift from Dr. Steven Vogel, Addgene #27795), and mPI31 CDS was cloned from a mouse cDNA library ...
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bioRxiv - Molecular Biology 2019Quote: ... ΔPPXY)-T2A-GFP were generated by PCR from pCDNA4/TO-HA-NLS-SV40-B-MYB (this work) and pSpCas9n(BB)-2A-GFP (PX461) (Addgene #48140). All entry vectors were subcloned into pENTR3C (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... NLS-mCherry-LEXY constructs are generated by ligation of the NLS-mCherry-LEXY insert part PCR amplified from a mammalian expression vector (Addgene #7265511) and a fly expression vector pBabr-mTub-MCS-sqh3’UTR (courtesy from Wieschaus lab ...
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bioRxiv - Physiology 2021Quote: ... The nls-sfGFP and yki[S3A]-sfGFP PCR products were then cloned by Gibson assembly in the backbone of the pJFRC19-13XLexAop2-IVS-myr::GFP plasmid (Addgene – 26224) previously digested with XbaI and XhoI to remove the myr::GFP sequence ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SNAP-GLP-1R-Nluc was generated in house by PCR cloning of the nanoluciferase sequence from pcDNA3.1-ccdB-Nanoluc (a gift from Mikko Taipale; Addgene plasmid # 87067) onto the C-terminus end of the SNAP-GLP-1R vector (Cisbio) ...
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bioRxiv - Neuroscience 2022Quote: pFUGW-NLS-FlpO was created using a PCR reaction for NLS-FlpO with primers AJ20130 - AJ20131 using pAAV hSynapsin FlpO (Addgene #60663), a gift from Massimo Scanzianis (Xue ...
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bioRxiv - Cell Biology 2020Quote: ... was made by amplifying TPD54 by PCR from human Tumor protein D54 (IMAGE clone: 3446037) and inserting into pIRES-EGFP-puro (Addgene #45567) via NheI and XhoI ...
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bioRxiv - Cell Biology 2021Quote: ... the cDNA sequence encoding the third generation rtTA protein (rtTA3) was obtained by PCR from plasmid MXS_PGK::rtTA3-bGHpA (Addgene plasmid #62446) (Sladitschek and Neveu ...
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bioRxiv - Immunology 2021Quote: ... Binding sites for the restriction enzyme AscI were added to the 5’ and 3’ ends of the construct by PCR and used to insert the mCAR sequence into the Rosa26-targeting vector CTV [(Addgene #15912), Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... inverted CD9-EGFP from the pcDNA3.1 plasmid was amplified by PCR and ligated into pCAG-Cre (a gift from Connie Cepko; Addgene plasmid # 13775) digested with EcoRI and NotI (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... The ZIKV sfRNA sequence and GFP gene fragment were PCR amplified from pUC19-ZIKV-F4 14 and pMYC-GFP (Addgene, #42142) plasmids respectively using primers HindIII_ZIKV_sfRNA and XbaI_GFP (Supplementary table 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... and ΔC (251-545 aa) truncation domains were PCR amplified and cloned into pET Strep II TEV LIC cloning vector (1R, Addgene #29664). The genes encoding Csb1/I-G ...
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bioRxiv - Biochemistry 2022Quote: ... expression plasmid was cloned by PCR amplification from full length ScTop2 12URA-B vector followed by insertion into a modified 1-B (Addgene #29653) E ...
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bioRxiv - Biochemistry 2022Quote: An insert of ZZUD and ZZUDL1340P were cloned out from a positive pGEX-6P-1-ZZUD or pGEX-6P-1 - ZZUDL1340P with PCR based cloning procedure into a pEGFP-C1 (Addgene 54759) or mRFP-C1 (Addgene 54764 ...
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bioRxiv - Cell Biology 2022Quote: GLP-1R-NanoLuc was generated in house by PCR cloning of the NanoLuciferase sequence from pcDNA3.1-ccdB-NanoLuc (a gift from Prof Mikko Taipale; Addgene plasmid # 87067) onto the C-terminus end of the SNAP-GLP-1R vector (CisBio) ...
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bioRxiv - Cell Biology 2022Quote: ... The mammalian expression construct for mouse MLXIPL was generated by PCR-amplification from a template plasmid (from the laboratory of Isabelle Leclerc, Addgene #39235) and cloned into pcDNA3 with an N-terminal FLAG epitope tag by Gibson assembly ...
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bioRxiv - Immunology 2022Quote: ... pTRIP-SFFV-Hygro-2A-mScarlet-VPS4A E228Q was obtained by Gibson assembly of PCR amplified VPS4A E228Q from pEGFP-VPS4-E228Q (Addgene #80351). pTRIP-SFFV-Hygro-2A-mScarlet-UBAP1DN and pTRIP-SFFV-Puro-2A-mScarlet-UBAP1DN (truncated mutant at residue 97 - mutation G98X ...
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bioRxiv - Immunology 2022Quote: ... pTRIP-hPGK-Blast-2A was cloned from pTRIP-CMV-STING-GFP (kind gift of Nicolas Manel) by Gibson assembly of PCR amplified hPGK from pCW57-MCS1-2A-MCS2 (Addgene #71782) and a gBlock (IDT ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’-ITR was amplified from PB- empty with Gibson overhangs and both PCR products were assembled into the pUC19 vector (Addgene, #50005) to yield pUC19-lHA-5’ITR ...
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bioRxiv - Synthetic Biology 2022Quote: ... This was achieved by linearizing the backbone with MluI and then performing Gibson assemby [59] with the four additional SunTags that were PCR amplified from pCAG-dCas9-5xPlat2AflD (Addgene, #82560).
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bioRxiv - Neuroscience 2022Quote: ... FRT10-stop-FRT10 was PCR amplified from pUAS-FRT10-stop-FRT10-mCD8-GFP (Li et al., 2021) and inserted into pUAS-3xHalo7- CAAX (Addgene #87646) through NotI and DNA assembly.
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bioRxiv - Neuroscience 2023Quote: ... Then each intact gRNA expression cassette was amplified by PCR and both were inserted into an AAV expression vector that contained a EGFP-KASH reporter (pOTTC1553, Addgene #131682), resulting in plasmid pOTTC1642 (Addgene #195018) ...
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bioRxiv - Biophysics 2022Quote: ... The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group ...
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bioRxiv - Neuroscience 2023Quote: ... Human Synapsin1 promoter and smFP-HA were PCR amplified from pAAV-hSyn-EGFP (a gift from Bryan Roth, Addgene Plasmid #50465) and pCAG_smFP-HA (a gift from Loren Looger ...
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bioRxiv - Cell Biology 2023Quote: ... The oDi sequence encoding a coiled-coil dimerization domain was amplified by PCR (#503/#504) from SpyCatcher002-oDi (a gift from Mark Howarth, Addgene # 124661) (Khairil Anuar et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the oTet sequence encoding a tetramerization coiled-coil domain was obtained by PCR (#505/#506) from SpyCatcher002-oTet (a gift from Mark Howarth, Addgene # 124663) (Khairil Anuar et al. ...
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bioRxiv - Molecular Biology 2023Quote: The spectinomycin resistance cassette (promoter, aadA CDS and terminator) was PCR-amplified from pAGM1299 (Addgene plasmid # 47988; (Weber et al., 2011)) using Phusion high-fidelity DNA polymerase (New England Biolabs Ltd ...
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bioRxiv - Cell Biology 2023Quote: ... Mapt exon 4 and flanking the intron regions were PCR amplified using Phusion High-Fidelity DNA polymerase and inserted into NheI/BamHI digested pFlareA Plasmid (Addgene, 90249) and sequenced ...
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bioRxiv - Synthetic Biology 2023Quote: ... Synthetic enhancers were amplified by PCR with primers that included homology to the plasmid vector E1b-GFP-Tol2 (Addgene plasmid #37845)81 and were cloned upstream of the minimal promoter (E1b ...
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bioRxiv - Bioengineering 2023Quote: ... the scFv-GCN4-linker-VP16-GB1-Rex NLS sequence was PCR amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904) and cloned into a lentiviral backbone containing an EF1-alpha promoter ...
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bioRxiv - Neuroscience 2023Quote: ... the transgene p130PH or p130PHR134L were amplified using PCR from the pEGFP-N1 plasmids containing these genes31 and inserted into the Ef1α-DIO-mCherry viral vector (Addgene, #47636). AAVTM6 viruses were produced at Janelia Viral Tools facility ...
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bioRxiv - Molecular Biology 2023Quote: Polymerase chain reaction (PCR) was used to isolate mouse AMPKγ1 cDNA from a mouse AMPKγ1-HA vector (#40605, Addgene, Watertown, MA, USA). Mouse AMPKγ1 cDNA was then cloned into the 3xFLAG-CMV10 vector (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... The P2A-mScarlet fragment was obtained by PCR cloning from pEB2-mScarlet (a kind gift from Dr. Philippe Cluzel; Addgene #104006)(Balleza et al. ...
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bioRxiv - Cell Biology 2022Quote: ... digesting the PCR generated fragment with HindIII and BamHI restriction enzymes and finally cloning it into the ptdTomato-C1 vector (Addgene, #54653), previously digested with HindIII and BamHI restriction enzymes.
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bioRxiv - Cell Biology 2023Quote: ... PH-FFAT/N-PH-FFAT sequences (5’-NheI/3’-AscI) were amplified by PCR from pLJM1-FLAG-GFP-OSBP plasmid (Addgene#134659). The sequences encoding Twitch (Twitch2b/Twitch7x/Twitch8x/Twitch9x ...
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bioRxiv - Cell Biology 2023Quote: ... the strain expressing Hfl1-NG was produced from strain Y1508 by introducing a DNA fragment obtained by PCR using plasmid pFA6a-link-ymNeongreen-SpHis5 (Addgene, #125704) and oligonucleotides #1706 ...
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bioRxiv - Cell Biology 2023Quote: pENTR H2B mScarlet-I was created by PCR amplification of mScarlet-I from plasmid EB3- mScarlet-I (Addgene #98826, Dorus Gadella), using the primers FW 5’- ACCGGTGAATTCACCATGGTGAGCAAGGGCG-3’ and RV 5’-
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bioRxiv - Plant Biology 2023Quote: A pair of oligos containing the gRNA sequence were used in conjunction with vector-specific primers (Table S1) for PCR amplification of Medicago truncatula U6 promoter and scaffold from the pUC-gRNA plasmid (Addgene #47024) using Q5® High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... The construct encoding SARS-CoV-2 matrix (M) protein was cloned by PCR amplification of M cDNA from the pDONR 207 SARS-CoV-2 M plasmid (Addgene #141274) using a Forward primer inserting a restriction site for EcoRI and a reverse primer bearing a restriction site for BamHI and a Myc tag ...
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bioRxiv - Synthetic Biology 2023Quote: ... The ERdd GFP LEU2 donor plasmid SHe146 (Supplementary Data S4) was cloned by Golden Gate assembly from parts PCR amplified from pScDD2_ERdd (Addgene plasmid #109047), pWS082 (Addgene plasmid #90516) ...
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bioRxiv - Developmental Biology 2023Quote: An enhanced piggyBac Puromycin selectable and DOX inducible vector was digested with EcoRI-NotI and ligated with PCR-amplified human SOX2 from the FUW-tetO-hSOX2 plasmid (Addgene#20724). Transfection into hPSCs was done using Lipofectamine Stem Reagent per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... we amplified a 10xHis-MBP coding fragment by PCR with primers pair 10xHis-MBP-F and 10xHis-MBP-R from pMAL-c2X® vector (Addgene), and cloned it into pTrcHis® vector (Addgene ...
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bioRxiv - Genetics 2023Quote: ... We generated SiT-ddCas12a-[Repr] by introducing the DNase-inactivating E993A by PCR-based mutagenesis using SiT-Cas12a-[Repr] (Addgene #133568) as template ...
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bioRxiv - Cell Biology 2023Quote: pLVX-EF1a-H2B-emiRFP703-neo was created by PCR of H2B-emiRFP703 from pH2B-emiRP703 (a gift from Vladislav Verkhusha, AddGene #136567) with forward primer (with prepended NotI digest site ...
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bioRxiv - Cell Biology 2024Quote: To construct the ER-GCaMP fused to mRuby3, mRuby3 was PCR amplified from the pKanCMV-mRuby3-18aa-actin (Bajar et al., 2016) (Addgene #74255). CMV ER-GCaMP6-210 was restriction digested with AgeI-HF and combined with the PCR amplified mRuby3 via ligation ...
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bioRxiv - Neuroscience 2024Quote: ... that was first PCR amplified using custom primers (see Table 2) from a plasmid containing lexAop2 (lexAop2-myr-4xSNAPf, RRID: Addgene 87638) and then restriction digested using HindIII and PspXI (New England BioLabs ...