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Citations for Addgene :
751 - 800 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T cells were transfected with lenti-ZsGreen (Addgene) and 10 µg of psPAX2 (Addgene ...
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bioRxiv - Immunology 2019Quote: ... A375 cells were transduced with lentiCas9-Blast (Addgene) and selected with 5 μg/mL blasticidin for 10 days ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with CMV-R_GECO (Addgene #45494) (Wu et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells stably expressing inducible Snail (Addgene plasmid #18798), constitutively active p38 or MAPK14 gRNA targets were generated by transduction of cells as described previously (Mani et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were co-transfected with HRE-luc (Addgene) (Emerling ...
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bioRxiv - Biophysics 2020Quote: ... we transfected LentiX cells with psPAX2 (Addgene #12260), pMD2.G (Addgene #12259) ...
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bioRxiv - Immunology 2022Quote: HEK293A cells stably expressing mouse NLRP3 (Addgene 75127), and ASC-GFP (Addgene 73957 ...
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bioRxiv - Genetics 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 μg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... CaCl2 transformation of JM83 cells obtained from Addgene with thermally denatured targeted DNA and ssODN was performed as described by Mandecki except for using SOC media instead of 2x YT for Ampr marker expression ...
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bioRxiv - Cancer Biology 2019Quote: ... U2OS cells were transfected with pCBASceI (Addgene; #26477) in the presence of 1 µg/ml doxycycline ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with psPAX2 (Addgene), pMD2.G VSV-g and the eSpCas9-LentiCRISPRv2 construct targeting ZMPSTE24 or the IFITM proteins or a non-targeting control at a ratio of 0.6:0.9:0.6 using 1 mg/ml PEI®-MAX ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells stably expressing inducible Snail (Addgene plasmid #18798) or indicated reporter plasmids were generated by transduction of LNCaP95 or CS2 cells as described:
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bioRxiv - Genetics 2022Quote: DLD1 cells were transduced with 7TFC (Addgene#24307) lentivirus ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells containing pLenti-PE2-BSD (Addgene, #161514) were treated with 10 µg/ml blasticidin for selection of blasticidin and incubated for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then transfected with plasmids (9021,60360,139059 - Addgene) using the Lipofectamine 3000 DNA Transfection Reagent for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transduced with a pRDA_256 (Addgene #158581) co-expressing an sgRNA and BE3.9max with a puromycin resistance cassette ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with pLew100cre-del-tetO (Addgene) [66] and selected with 40 µg/mL ganciclovir ...
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bioRxiv - Cancer Biology 2023Quote: ... cell membrane marker (pLenti-mCherry-CAAX, Addgene #129285), E-cadherin reporter (pHAGE-E-cadherin-RFP ...
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bioRxiv - Cancer Biology 2023Quote: ... or GFP positive cells (when using Addgene #195124) were single cell sorted onto 96-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... cells were infected with pRDA_186 plasmid (Addgene 133458), a gift from John Doench (Broad Institute) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transiently transfected with PX459 (Addgene; #48139) and selected with puromycin for 2 days ...
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bioRxiv - Microbiology 2023Quote: ... PEL cell lines expressing lentiCas9-Blast (Addgene, 52962) were previously generated and validated by STR profiling7 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells transfected with EGFP-Actin (Addgene plasmid #31502) (52 ...
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bioRxiv - Cell Biology 2023Quote: PC12 cells were transfected with NPY-pHTomato (Addgene) and stably selected ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with GCaMP7s (Addgene Plasmid #104463)(14) ...
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bioRxiv - Systems Biology 2024Quote: ... BJ1 cells were transduced with Cas9 (Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days ...
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bioRxiv - Physiology 2023Quote: ... HEK293T cells were incubated with pMD2G (#12259, Addgene), psPAX2 (#12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... HPAF-II cells stably expressing FUCas9Cherry (Addgene#70182) were transduced with MSMO1 sg RNA or FAXDC2 sgRNA along with pMD2.G (Addgene #12259 ...
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bioRxiv - Systems Biology 2023Quote: ... Cell (2023) (also deposited in Addgene MORF Collection).
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bioRxiv - Biochemistry 2024Quote: ... coli Δlac cells (Addgene, Didovyk et al., 2017) transformed with plasmids encoding β-gal (wild-type or mutant ...
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bioRxiv - Genomics 2023Quote: ... LNCaP cells stably expressing dCas9-KRAB (Addgene #89567) were seeded in a 6-well plate at a density of 200K cells per well ...
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bioRxiv - Genomics 2023Quote: ... cells were transfected with pMD2.G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pMD2.G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Biochemistry 2024Quote: ... HT22 cells were transfected with psPAX2 (Addgene # 12260), and pMD2.G (Addgene # 12259 ...
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bioRxiv - Cancer Biology 2020Quote: Generation of batch MTR knockout cells in each cell line was achieved using the lentiviral CRISPR–Cas9 vector system LentiCRISPR v2 (Addgene #52961). Briefly ...
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bioRxiv - Biochemistry 2020Quote: HEK293T CRISPR-Cas9 cells were generated by transfecting wild-type 293T cells with pX330-U6-Chimeric_BB-CBh-hSpCas9 vector (Addgene, Plasmid# 42230) carrying single-guide RNA (sgRNA ...
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bioRxiv - Microbiology 2020Quote: RAW 264.7 cells stably expressing FL-Cas9 were generated by transducing RAW 264.7 cells with lentivirus containing LentiCas9-Blast (Addgene plasmid #52962)(Sanjana et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 STEAP1 ko and PC3 STEAP1 ko cells were generated by transient transfection of 22Rv1 cells with a pool of PX458 (Addgene, #48138) plasmids each expressing one of four different sgRNA targeting sequences predicted from the Broad Institute Genetic Perturbation Platform sgRNA Designer63 ...
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bioRxiv - Genomics 2020Quote: In order to create stable dCas9-KRAB expressing cell line LNCaP cells were infected with lenti-KRAB-dCas9-blast (Addgene, 89567) and selected with 6 µg/ml blasticidin for two weeks ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Cancer Biology 2022Quote: ... These constructs were introduced into cells generated by lentiviral transduction of MCF10A TP53-/- cells with lenti-BLAST-dCas9-KRAB (Addgene, #89567) followed by selection with 10 μg/mL blasticidin.
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bioRxiv - Cell Biology 2022Quote: An OgtfloxedCre-ERT2+/KI Rosa26-LSL-YFP+/KI mESC cell line stably expressing Cas9 was generated by lentiviral transduction of the parental cells with lentiCas9-Blast (Addgene #52962). CRISPR sgRNA library lentiGuide-Puro-Brie (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... LS174T ERN1-/- cells and LS174T ERN2-/- cells were constructed by transfection with pSpCas9(BB)-2A-GFP (Feng Zhang lab, Addgene #48138) containing sequences for guides targeting exon 8 (WGE ID’s 1150303698 and 1150303744 for ERN1 ...
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bioRxiv - Genetics 2023Quote: We plated 550,000 HEK293T cells on 6-well plates and 24 hours later we transfected the cells with 900 ng psPAX2 packaging vector (Addgene #12260), 360 ng pMD2.g VSV-G envelope vector (Addgene #12259) ...
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bioRxiv - Microbiology 2024Quote: HeLa-TetR-Cas9 clonal cells used in the genome-wide screen and polyclonal Huh7 cells transduced with 311-Cas9 (Plasmid Addgene #96924) and selected using 10 µg/mL blasticidin for 7 days were used for secondary screening ...
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bioRxiv - Cancer Biology 2021Quote: ... Flp-In 3T3 p53-/- cell line was generated by Crispr/Cas9 by co-transfecting Flp-In 3T3 cells with px330 p53 plasmid (Addgene Plasmid #59910) expressing Cas9 and sgRNA targeting mouse p53 and pmaxGFP plasmid (Lonza) ...
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bioRxiv - Cancer Biology 2020Quote: ... To generate inducible CRISPR-Cas9 GFPT2 KO cell lines, parental cells (H460, H157, Calu-1) were first infected by pCW-Cas9 plasmid (Addgene plasmid #50661), sorted by puromycin selection ...
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bioRxiv - Cell Biology 2021Quote: Stock of lentiviral particles were obtained by transfection of HEK293T cells (2×106 cells) with 2 μg of lentivector plasmid lentiCRISPRv2 (a gift from Feng Zhang, Addgene plasmid, 52961) expressing single guide RNA targeting an exon within ATG5 gene ...
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bioRxiv - Cancer Biology 2021Quote: ... For generation of KO cell line pools PC-9 and HCC827 cells were transduced with pKLV2-EF1a-Cas9Bsd-W (Addgene ID:68343)[71] to stably express Cas9 ...