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Citations for Addgene :
651 - 700 of 980 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Plasmids encoding GFP-2A-ORF6 were generated by inserting EGFP-T2A sequence (copied from Addgene #140424 by PCR) at the 5’ end of the ORF6 gene in pSecTag2 mammalian expression vector using Gibson Assembly ...
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bioRxiv - Molecular Biology 2023Quote: The DNA fragment encoding dLwaCas13a was PCR-amplified using pC035-dLwaCas13a-msfGFP (a gift from Feng Zhang; Addgene plasmid #91925 ...
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bioRxiv - Plant Biology 2023Quote: ... Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751 ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...
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bioRxiv - Cell Biology 2023Quote: ... with I532A mutation and RRRG degron) was amplified by PCR from pBMN-HA-YFP-LOV24 (Addgene, Plasmid #49570) (78 ...
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bioRxiv - Developmental Biology 2023Quote: The T7 promoter sequence was added to the Cas9 coding region by PCR amplification of px260 (Addgene, 42229) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of full-length synaptopodin was generated by PCR and subcloned into the pKH3 vector (Addgene). IP assays were performed basically according to the method described previously (59 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the T2A-EGFP sequence from pXR001 (Addgene #109049; a gift from Patrick Hsu44) using the primers T2A-GFP Gibson Forward and Reverse (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp90-GFP was first constructed by PCR amplifying HA-Hsp90 (a gift from William Sessa, Addgene plasmid #22487) and recombining into GFP pBabe puro digested with EcoRI-XhoI ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DNA fragments of TPPP FL and FKBP were produced by PCR using existing templates and (Addgene #31184). The tagBFP gene was synthesized as gBlocks (Integrated DNA Technologies) ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The resultant PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (Addgene # 29659) using in-fusion cloning kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: The full length BRD4 DNA fragment was amplified by PCR from pcDNA4-TO-HA-BRD4FL (Addgene plasmid #31351)61 incorporated into linearized FM5 lentiviral vectors containing standardized linkers (generously provided by David Sanders ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... This promoter sequence was PCR amplified with Mfe1 and Not1 and inserted into the pUB_smHA_KDM5B_MS2 (Addgene plasmid # 81085) lentiviral vector29 replacing the UbC promoter ...
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bioRxiv - Cell Biology 2024Quote: The AAVS1 destination vector was assembled by PCR amplification and Gibson assembly from the pDTA-TK from Robert Benezra’s group (RRID:Addgene_22677) (Nam and Benezra 2009 ...
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bioRxiv - Cancer Biology 2024Quote: ... mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’ ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting PCR fragments were cloned into pJUMP27 (pJUMP27-1AsfGFP was a gift from Chris French, Addgene plasmid #126974) (45 ...
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bioRxiv - Molecular Biology 2021Quote: ... a region bearing six let-7 binding sites was PCR-amplified from MSCV puro let-7 sponge (Addgene #29766) and cloned between AgeI and EcoRI sites downstream of GFP.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was digested with EcoRI and ligated into EcoRI digested pHR-SFFV-KRAB-dCas9 vector (Addgene, 60954), to generate a pHR-pTRE3G-KRAB-dCas9 vector ...
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bioRxiv - Neuroscience 2019Quote: ... The T2A-GFP sequence was amplified by PCR using plasmid PX461 (a gift from Feng Zhang; Addgene plasmid #48140) as template and inserted into the intermediate plasmid at HindIII site to generate pAAV-hSyn-FLPo-T2A-GFP ...
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bioRxiv - Synthetic Biology 2021Quote: ... multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283433) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus ...
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bioRxiv - Cell Biology 2020Quote: The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).
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bioRxiv - Neuroscience 2019Quote: GFP-progerin cDNA was PCR amplified from the pBABE-puro-GFP-progerin plasmid (Addgene, 17663 deposited by Tom Misteli) using primers (forward 5’-GATCATCGATATGGTGAGCAAGGGCGAGGAG-3’ ...
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bioRxiv - Biochemistry 2020Quote: BsaI sites and compatible overhangs were added by PCR amplification of cpGFP from pTKEI-Mal-B2 (Addgene Plasmid #79756) using primers cpGFP-BsaI-GG-F and cpGFP-BsaI-GG-R ...
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bioRxiv - Cell Biology 2021Quote: ... the open reading frames of mTagBFP2 and mNeongreen2 were amplified by PCR from donor vectors pBAD-mTagBFP2 (Addgene #34632) and pSFFV_mNG2(11)1-10 (Addgene #82610) ...
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bioRxiv - Microbiology 2021Quote: ... the human ACE2 coding sequence (RefSeq accession NM_001371415.1) was PCR amplified and cloned into the BamHi site of lentiviral vector pHR-PGK (Addgene #79120). Lentivirus was produced as described above and used to transduce 5×104 target cells (A549 ...
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bioRxiv - Systems Biology 2020Quote: ... and S415A) were introduced using the delitto perfetto method (Stuckey et al., 2011) using the PCR-amplified pCORE cassette (RRID:Addgene_72231) to integrate selective markers at the endogenous gene loci ...
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bioRxiv - Cell Biology 2021Quote: ... mScarlet-i-H2A construct was amplified by PCR from pmScarlet-i_H2A_C1 (a gift from Dorus Gadella, Addgene plasmid #85053) (Bindels et al. ...
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bioRxiv - Cell Biology 2021Quote: ... the EB3-tdTomato fragment was amplified by PCR from EB3-tdTomato (a gift from Erik Dent, Addgene plasmid #50708) (Merriam et al. ...
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bioRxiv - Neuroscience 2021Quote: ... TVA-mCherry was amplified by PCR from a plasmid (gift from Dr. Uchida, Addgene plasmid #38044 ; http://n2t.net/addgene:38044 ; RRID:Addgene_38044, ref43) and inserted into a 5xUAS vector34.
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bioRxiv - Developmental Biology 2022Quote: ... 100µl of the ESCs were then mixed with the scCRISPR construct (Composition: 10µl elute of HDR PCR product; 1µl sgPal7 (Addgene, 71484); 1µl spCas9-BlastR (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... NTR candidates were PCR amplified and cloned into the NdeI and SalI sites of two plasmids: pUCX (Addgene #60681), for biological overexpression assays ...
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bioRxiv - Neuroscience 2022Quote: ... the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ...
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bioRxiv - Cell Biology 2019Quote: ... The enhanced green fluorescent protein (EGFP) sequence was amplified via PCR from a pcDNA3-EGFP plasmid that was a gift from Doug Golenbock (RRID:Addgene_13031). The EGFP sequence was cloned in-frame on the N-terminus of TMEM135 in the pTarget vector using BamHI and XhoI restriction enzymes ...
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272; http://n2t.net/addgene:27272; RRID: Addgene_27272) (Amin et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... guide sequences were PCR amplified from a CustomArray Inc oligo pool and cloned into the lentiGuide-Puro (Addgene #52963) backbone using Golden Gate cloning ...
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Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extensionbioRxiv - Developmental Biology 2021Quote: ... and the cDNA of CRY2PHR was PCR amplified from the pCRY2PHR-mCherryN1 plasmid from the Tucker Lab (Addgene 26866) (7) ...
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bioRxiv - Molecular Biology 2021Quote: Full-length and C-terminal truncated KDM6B cDNA was amplified by PCR from KDM6B plasmids (Addgene, #21212 and #21214) and subcloned into pLVX-Ubc-FLAG vector ...
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bioRxiv - Biochemistry 2021Quote: A construct encoding residues 109-492 comprising soluble TMPRSS2 ectodomain was amplified by two PCR fragments (Addgene plasmid# 53887) and subcloned into the pFHMSP-LIC C donor plasmid by LIC method ...
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bioRxiv - Microbiology 2020Quote: ... the human ACE2 gene (Miaolingbio #P5271) was PCR-amplified and cloned into the pLV-EF1a-IRES-blast (Addgene #85133). The human TMPRSS2 and DPP4 gene (Sino Biological #HG13070-CM ...
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bioRxiv - Molecular Biology 2022Quote: ... Flag-hHOXA1 (pchHOXA1-3XFlag-myc) and Flag-mHoxA1 (pcmHoxa1-3XFlag-myc) were generated by cloning PCR-amplified inserts into a pcDNA3.1 vector (Addgene) featuring a 3XFlag-myc tag sequence in the multiple cloning site ...
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bioRxiv - Biophysics 2022Quote: ... the DNA sequence encoding the T7 RNA polymerase promoter and sgRNA was amplified by PCR from pHelper_ShCAST_sgRNA vector (Addgene, #127921). The DNA template was then subjected to GeneJet PCR purification (ThermoScientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Histone H2A cDNA were PCR amplified from pCDNA3.1-Flag-H2A and pCDNA3.1-Flag-H2A K118-119R51 (Addgene, #63560, #63564). Histones Macro-H2A cDNAs were obtained for the DKFZ cDNA clone repository.
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bioRxiv - Immunology 2022Quote: ... pTRIP-hPGK-STING-TurboID was cloned by Gibson assembly of PCR amplified TurboID from V5-TurboID-NES_pCDNA3 (Addgene #107169) and STING from pTRIP-CMV-STING-GFP ...
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bioRxiv - Plant Biology 2022Quote: ... The LacZ selection marker was PCR amplified with flanking BsaI sites into Level 1 acceptor vector pICH41780 (Addgene #48019) to allow blue-white screening for successful gRNA insertion into final ABE vectors ...
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bioRxiv - Synthetic Biology 2022Quote: ... This fragment was subcloned with the above PCR fragment using Gibson enzymatic assembly (Gibson et al. 2009) to generate PRE-Hsp70BbCas9_1.0 (Addgene 190795). Gypsy insulator elements were subsequently cloned into PRE-Hsp70BbCas9_1.0 through two Gibson cloning events to generate PRE-Hsp70BbCas9_1.2 (Addgene 190796 ...
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bioRxiv - Molecular Biology 2023Quote: ... the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into pET-His6-MBP-TEV (Addgene #29656) by ligation-independent cloning following QB3 Macrolab protocols (https://qb3.berkeley.edu/facility/qb3-macrolab/) ...
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bioRxiv - Molecular Biology 2023Quote: ... each of these systems was PCR amplified and cloned into pTNS2 to replace the parental mini-Tn7 (Addgene #64968) by Golden Gate assembly ...
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bioRxiv - Developmental Biology 2023Quote: ... was cloned with Gibson assembly in frame with the SV40pA sequences that were PCR amplified from lentiCRIPSRv2 (Addgene, #52961). Gibson cloning was subsequently used to simultaneously encompass digested 8xtetO-EF1a promoter-eGFP-SV40pA cassette in frame with the homology arms and the whole insert was cloned into the pSMART-HCKAN (Lucigen ...
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bioRxiv - Biochemistry 2023Quote: ... at AgeI and NheI sites and fusing PCR amplified CIB1 and spTN (Addgene plasmid # 153002, (Cho et al., 2020a)) using Gibson assembly ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the U6-crRNA scaffold and the DiCas7-11 gene sequence from pDF0114 (Addgene #172508) and pDF0159 (Addgene #172507) ...