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Citations for Addgene :
651 - 700 of 2187 citations for Rat Epithelial Discoidin Domain Containing Receptor 1 DDR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and a plasmid containing both an empty sgRNA scaffold as well as a gene sequence encoding SpCas9 (AddGene plasmid# 57819) with a final goal of LV positive control vector generation ...
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bioRxiv - Immunology 2023Quote: ... The lentiCRISPRv2-puro vector containing the Cas9 expression cassette and the gRNA scaffold was a gift from Brett Stringer (Addgene plasmid #98290 ...
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bioRxiv - Biochemistry 2023Quote: ... human EZH2 wildtype and the K20R or S21A mutant of human EZH2 were cloned into the retroviral pMSCV-Puro vector containing 3xFlag-3xHA epitope (Addgene) and the recombinant retroviruses were packaged in 293 cells (Guo et al. ...
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bioRxiv - Bioengineering 2023Quote: The pSECRETS-C plasmids containing desired on-target sequences were constructed via HiFi assembly into the p11.LacY.wtx1 plasmid (Addgene #69056), which was double digested with XbaI and SphI ...
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bioRxiv - Neuroscience 2023Quote: ... A virus lacking the hM4Di DREADD gene and instead containing the fluorescent tag eGFP (AAV8-CaMKIIa-EGFP, packaged by Addgene) was infused into ACC in 4 animals as a null virus control ...
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bioRxiv - Cell Biology 2023Quote: The human sFLT1-HA plasmid construct created through Gibson cloning of human sFLT1 cDNA into a pcDNA3.1 vector containing a C-terminal HA tag (pcDNA3-ALK2-HA, Addgene 80870). Sequencing validation was performed through Genewiz ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-line(#4954) was transduced using viral particles containing the lentiCas9-Blast vector (Addgene, Watertown, MA; RRID:Addgene_52962). Cells stably expressing Cas9 were selected using 10ug/ml Blasticidin S HCl (Gibco™-#R21001) ...
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bioRxiv - Biophysics 2023Quote: ... 3C protease site cloned into a pcDNA3 vector containing human IgG Fc derived from pcDNA3-Nrxn1beta AS4(-)-Fc (a gift from Peter Scheiffele & Tito Serafini, Addgene plasmid # 59313 ...
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bioRxiv - Microbiology 2023Quote: ... A fragment of MeCP2 containing the T158M mutation was amplified by PCR and cloned in pDEST-hMeCP2-GFP (a gift from Huda Zoghbi, Addgene plasmid # 48078 ...
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bioRxiv - Biochemistry 2023Quote: Mass spectrometry sample preparation: N2A cells were transfected with pcDNA3 plasmids containing 2X myc-EXOSC3 or pcDNA3 empty vector (Addgene) using Lipofectamine® 2000 (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... A 250 µL aliquot of lentiviral cocktail containing either Lenti-Cas9-Blast plasmid (SU-DIPG-XIII; Addgene, Watertown, MA, USA) or Lenti-Cas9-2A-Blast (SU-DIPG-XXXVI ...
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bioRxiv - Biochemistry 2023Quote: A pET29a-YS14 plasmid containing Xenopus laevis histones was obtained as a gift from Jung-Hyun Min (Addgene plasmid # 66890). H2A-H2B was subcloned into pET His6 TEV LIC cloning vector (2B-T ...
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bioRxiv - Biochemistry 2023Quote: Phosphorylated tauC3 was expressed in Sf9 insect cells from a 438B pFastBac vector containing an engineered 6xHis tag and a TEV protease cleavage site (Addgene). Following two rounds of virus amplification ...
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bioRxiv - Cell Biology 2023Quote: Entry clones were transferred using Gateway technology into a mammalian expression pcDNA3.1 plasmid containing an N-terminal 3xFLAG-V5 tag (Addgene 87064) for the Target Accelerator Pan-Cancer Mutant Collection ...
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bioRxiv - Synthetic Biology 2023Quote: ... construct was made by also acquiring a lentiviral transfer plasmid containing the GAL4-VP64 inducible upstream activator sequence (UAS) from Addgene (pHR_5x Gal4 UAS was a gift from Wendell Lim (Addgene plasmid # 79119 ...
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bioRxiv - Immunology 2023Quote: ... PH5CH and Huh7-Lunet-TLR3 cells stably expressing the tBID death reporter and Cas9 were transduced with lentiviral vectors containing the GeCKOv2.0 human genome-wide sgRNA library (Addgene, USA) at MOI=0.3 ...
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bioRxiv - Cell Biology 2023Quote: ... The pCAG-V5-TurboID vector was constructed by inserting the amplified TurboID cDNA containing the V5 epitope (GKPIPNPLLGLDST) sequence at the N-and C-terminal sites into the pCAGGS expression plasmid (Addgene). Expression plasmids encoding V5-TurboID-Solo and Solo-TurboID-V5 were constructed by inserting amplified Solo cDNA with a GS-linker (GGGSx2 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of pX330 plasmid containing sgRNA for the target protein and 0.2 µg of pcDNA3-FKBP-eGFP-HOTag3 (Addgene, #106924) were co-transfected using Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... UBA5) were generated as described above with the following modifications: third-generation packaging DNA mix containing pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Neuroscience 2023Quote: ... the plasmid to be packaged was co-transfected into HEK293T cells with a rep/cap containing plasmid pUCmini-iCAP-PHP.eB (Addgene #103005) and the helper plasmid pAdDeltaF6 (Addgene #112867) ...
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bioRxiv - Neuroscience 2024Quote: ... A pulled glass pipette tip of 20–30 μm containing CTB647 (ThermoFischer Scientific, C34778) or retrograde AAV (Addgene, AAV-PHP.eB) and FG was lowered into the brain ...
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bioRxiv - Neuroscience 2024Quote: ... and a micropipette containing 100nL of pAAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (Addgene 71760-AAV1; titer: 9.8×1012vg/mL) was injected unilaterally into the VTA of DAT-IRES-Cre mice.
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bioRxiv - Cell Biology 2023Quote: ... to co- transfect HEK 293FT cells with plasmids containing the packaging gag/pol and envelope pCMV- 435VSV-G genes and either the pMRX-IB-HaloTag7-mGFP-KDEL (Addgene, 184904 ...
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bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
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bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...
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bioRxiv - Plant Biology 2020Quote: ... from Sylvestre Marillonnet (Addgene kit # 1000000044). Specific parts (target gRNA or siRNA sequence ...
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bioRxiv - Synthetic Biology 2022Quote: The MoClo Toolkit (Addgene Kit #1000000044), MoClo Plant Parts Kit (Addgene Kit #1000000047) ...
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bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
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bioRxiv - Genetics 2023Quote: ... and pX330S-2 (Addgene Kit 1000000055) according to the kit instructions ...
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bioRxiv - Bioengineering 2024Quote: ... including existing parts (Addgene Kit # 1000000080) and custom-made parts (Table 2) ...
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bioRxiv - Biochemistry 2024Quote: ... a ∼1:1:1 molar ratio mixture of library transfer (lentiCRISPRv2; Addgene plasmid #52961) and packaging plasmids (psPAX2 ...
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bioRxiv - Neuroscience 2021Quote: ... DJ-1 (pGEX-5X-1-DJ1-WT; Addgene) or pcDNA plus RFP plasmid(57 ...
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bioRxiv - Cell Biology 2020Quote: ... obtained from Dharmacon) into pUAST vectors containing a C-terminal Venus open reading frame (Wang et. al, 2012, Addgene plasmid 35204). Transgenes were sequenced and then injected into embryos containing an attP8 site on the X chromosome (BDSC stock #32233 ...
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bioRxiv - Microbiology 2020Quote: ... A DNA fragment containing a codon-optimized version of hACE2 (Genbank NM_021804) was inserted into pLV-EF1a-IRES-Hygro (Addgene Plasmid #85134) using Gibson assembly ...
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bioRxiv - Microbiology 2022Quote: ... were introduced into a previously described spike-expression plasmid containing D614G and a 21-amino-acid deletion in the cytoplasmic tail (Addgene 158762) (34) ...
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bioRxiv - Neuroscience 2022Quote: ... 90ul cells suspension containing 1M cells was mixed with 10 uL DNA mix: 4 ug pSpCas9(BB)-2A-Puro (PX459) plasmid (Addgene #48139), • 0.4 ug gRNA encoding plasmid (pKLV-U6gRNA(BbsI)-PGKzeo2ABFP ...
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bioRxiv - Immunology 2019Quote: We cloned template DNA for the indicated guide RNAs into a pLX-sgRNA construct additionally containing blasticidin resistance (Addgene plasmid #50662). We transfected constructs into HEK293T cells along with lentivirus packaging vector pSPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Molecular Biology 2019Quote: ... N-terminal 3xFLAG_V5_GFP-tagging of endogenous nxf2 and panoramix loci was done by co-injecting a repair template and the gRNA containing plasmid (Addgene 45956) into act-Cas9 flies (BL-58492) ...
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bioRxiv - Synthetic Biology 2019Quote: Plasmids containing different TEV protease versions were episomally introduced in a pRSII413 vector which was a gift from Steven Haase (Addgene #35450). Transformed cells containing the HIS3 gene were selected on SMM plates (SMM with 20 g/L agar ...
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bioRxiv - Molecular Biology 2019Quote: ... Retroviral vectors for JMJD6 expression were generated by cloning the Jmjd6 coding sequence with a C-terminal FLAG-tag sequence (11) into the pBABE plasmid vector containing the neomycin resistance gene (Addgene #1767). Mutant versions of JMJD6 were generated from the wild type construct using QuikChange Lightning site-directed mutagenesis kit (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... The pCMV(CAT)T7-SB100 containing the CMV promoter and SB100X transposase was a gift to Addgene from Zsuzsanna Izsvak (Addgene # 34879) 78 ...
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bioRxiv - Cell Biology 2019Quote: ... The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397) as a gift from David Liu [31] ...
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bioRxiv - Developmental Biology 2019Quote: ... p118 PB-TRE-dCas9-VPR 70 containing dCas9 fused to VP64-p65-RTA (dCAS9-VPR) transcriptional activators and hygromycin resistance gene (Addgene, #63800) and a plasmid coding for the PiggyBac transposase (kind gift from Valentina Carlini) ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... 1 pmol of the oligo mix was added to a 20 μl Golden Gate reaction containing 100 ng destination plasmid (pATT-DEST, Addgene #79770), 10 units BsaI (NEB #R3733) ...
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bioRxiv - Neuroscience 2019Quote: ... Oligo nucleotides containing CRISPR target sequences (5’-CCGGCCGGGCCTACGGCTTG-3’) were annealed and ligated into pSpCas9 (BB)-2A-GFP (PX458) (Addgene 48138). Then ...
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bioRxiv - Microbiology 2020Quote: RAW 264.7 cells stably expressing FL-Cas9 were generated by transducing RAW 264.7 cells with lentivirus containing LentiCas9-Blast (Addgene plasmid #52962)(Sanjana et al. ...
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bioRxiv - Systems Biology 2021Quote: All sgRNAs were synthesized as individual oligo pairs (IDT) and cloned into a barcode library containing plasmid pool (pBA571, Addgene #85968), thereby linking each sgRNA to a unique guide barcode contained within the 3′-UTR of the puromycin resistance gene (33) ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were transfected with a mix of 8 μg lentiviral lentiCRISPRv2 vector containing the TKOv3 gRNA library 21 (Addgene #90294), 4.8 μg packaging vector psPAX2 ...
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bioRxiv - Neuroscience 2020Quote: ... with a pEGFP-C1 mammalian vector containing full length human A53T variant αSyn with a fusion EGFP tag (Addgene, Plasmid #40823), following the manufacturer’s protocol ...