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601 - 650 of 2982 citations for 1 4 Bis acetyloxy 3 dodecylsulfanyl 2 naphthyl acetate since 2020

• A ventral hippocampal-lateral septum pathway regulates social novelty preference

  Maha Rashid, et al., bioRxiv - Neuroscience 2024

  Quote: ... 750 nl of the retroAAV-hsyn-Cre (500nL, Addgene Lot v70508, 3*1013) was injected in the VTA of male and female C57/BL6 mice ...
• A modular circuit architecture coordinates the diversification of courtship strategies in Drosophila

  Rory T. Coleman, et al., bioRxiv - Neuroscience 2023

  Quote: ... and the p10-3’UTR PCR amplified from the plasmid pJFRC81 (Addgene #36432). These were then cloned by Gibson assembly (NEB ...
• Disinhibition enables vocal repertoire expansion after a critical period

  Fabian Heim, et al., bioRxiv - Neuroscience 2024

  Quote: ... pAAV-mDlx-ChR2-mCherry-Fishell-3 was a gift from Gordon Fishell (Addgene plasmid # 83898 ...
• A high-content RNA-based imaging assay reveals integrin beta 1 as a cofactor for cell entry of non-enveloped hepatitis E virus

  Rebecca Fu, et al., bioRxiv - Microbiology 2023

  Quote: S10-3 cells were transfected with the pSpCas9(BB)-2A-GFP (Addgene #48138) plasmid encoding the Cas 9 protein fused to GFP by the 2A peptide ...
• A high-throughput screening approach to discover potential colorectal cancer chemotherapeutics: Repurposing drugs to disrupt 14-3-3 protein-BAD interactions

  Siyi He, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 14-3-3ζ was subcloned into pmTurquoise2-N1 (Addgene, Massachusetts, USA; plasmid # 54843) using restriction enzymes EcoRI (NEB ...
• PME-1 suppresses anoikis and is associated with therapy relapse of PTEN-deficient prostate cancers

  Anna Aakula, et al., bioRxiv - Cancer Biology 2022

  Quote: ... PC-3 cells were transduced with lentivirus containing lentiCas9-Blast construct (Addgene #52962) and selected with growth medium containing 4 µg/ml blasticidin for approximately one week ...
• Genetic analysis of rab7 mutants in zebrafish

  Daniel Heutschi, et al., bioRxiv - Genetics 2023

  Quote: ... and a poly-A p-3’entry clone (Addgene, Kristen Kwan, Chien lab) were used.
• Candida glabrata maintains two Hap1 homologs, Zcf27 and Zcf4, for distinct roles in ergosterol gene regulation to mediate sterol homeostasis under azole and hypoxic conditions

  Debasmita Saha, et al., bioRxiv - Genetics 2024

  Quote: ... and 3’ UTR was PCR-amplified and cloned into the pGRB2.0 plasmid (Addgene) (73 ...
• Gut Bacteria Encode Reductases that Biotransform Steroid Hormones

  Gabriela Arp, et al., bioRxiv - Microbiology 2024

  Quote: ... The amplification product was inserted into the expression vector backbone (Addgene, 69864-3). A gibson assembly was performed on the restricted backbone and the amplification product (NEB ...
• Exploring memory-related network via dorsal hippocampus suppression

  Xu Han, et al., bioRxiv - Neuroscience 2024

  Quote: ... AAV8-hSyn-hM4Di-mCherry (1μL per site, titer ≥ 3 ×1012 vg/mL, Addgene) was stereotactically injected into the dHP (coordinates ...
• A massively parallel in vivo assay of TdT mutants yields variants with altered nucleotide insertion biases

  Courtney K. Carlson, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... The TdT gene was cloned into a pET28a expression vector (Addgene #69864-3) with an N-terminal 10xHis tag ...
• Red light responsive Cre recombinase for bacterial optogenetics

  Fereshteh Jafarbeglou, Mary J. Dunlop, bioRxiv - Synthetic Biology 2024

  Quote: ... The ho1-pcy gene was derived from the pNO286-3 plasmid (Addgene #107746) that was deposited by Ong et al.59 ...
• Time-tagged ticker tapes for intracellular recordings

  Dingchang Lin, et al., bioRxiv - Neuroscience 2021

  Quote: ... 4 μg of the second-generation packaging plasmid psPAX2 (Addgene; 12260), and 2 μg of viral entry protein VSV-G plasmid pMD2.G (Addgene ...
• Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila

  Gabriel T. Koreman, et al., bioRxiv - Genetics 2020

  Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235). A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
• Discrete TrkB-expressing neurons of the dorsomedial hypothalamus regulate feeding and thermogenesis

  Jessica Houtz, Guey-Ying Liao, Baoji Xu, bioRxiv - Neuroscience 2020

  Quote: ... AAV8-hSyn-DIO-hM3-mCherry (4×1012 vg/ml, Addgene 44362), AAV8-hSyn-hM4-mCherry (6×1012 vg/ml ...
• MAGIC: Mosaic Analysis by gRNA-Induced Crossing-over

  Sarah E. Allen, et al., bioRxiv - Genetics 2020

  Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235) using the oligonucleotides aaaaaaaaatcaaaATGAGCGGTACCGATTACAACAAAAGGAGTAGTGTGAG and GCCGACTGGCTTAGTTAattaattctagaTTAAAGCGAGTAGTGGGAGATGTTG ...
• Conjunctive vector coding and place coding in hippocampus share a common directional signal

  Yue-Qing Zhou, et al., bioRxiv - Neuroscience 2023

  Quote: ... WPRE.SV40 virus (titer: 4 × 1013 GC per ml, obtained from Addgene) was lowered to a depth of 2.4 mm below the dura ...
• Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems

  Aakaanksha Maddineni, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Lenti dCAS-VP64_Blast (4) was a gift from Feng Zhang (Addgene plasmid # 61425 ...
• Cortical circuits for cross-modal generalization

  Maëlle Guyoton, et al., bioRxiv - Neuroscience 2024

  Quote: ... and AAV2.CAG.tdTomato (Addgene, #59462-AAV2, titer: 4×10¹² vg/mL) were used ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: UAS-SARS-CoV-2-ORF3a transgenic flies were generated by PCR-mediated subcloning of the SARS-CoV-2-ORF3a coding sequence (pDONR207-SARS-CoV-2 ORF3a, #141271, Addgene) into pUASt-Attb (EcoRI/XbaI) ...
• SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1α in pulmonary epithelial and endothelial cells

  Magdalena Ambrożek-Latecka, et al., bioRxiv - Immunology 2023

  Quote: ... pDONR207 SARS-CoV-2 M and pDONR207 SARS-CoV-2 ORF3a (all plasmids were gifts from Fritz Roth via Addgene, # 141273 ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: pCMV-GFP-SARS-CoV-ORF3a was generated by recombining the SARS-CoV-2-ORF3a coding sequence (pDONR207 SARS-CoV-2 ORF3aA, #141271, Addgene) into pDEST-CMV-N-EGFP (#122842 ...
• Novel mechanism of inflammatory activation by Ebola virus matrix protein linked to the ebolavirus virulence

  Satoko Yamaoka, et al., bioRxiv - Microbiology 2024

  Quote: The following open reading frames were cloned into an expression vector under the control of the phosphoglycerate kinase promoter: IKK-2 and its mutant IKK-2 K44M (Addgene plasmids #11103 and #11104 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... equal amounts of both pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid DNA and pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... along with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... and pmNG-Mpro-NLuc-PLpro-mScarlet either alone or co-transfected with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with DuProSense biosensor containing Mpro cleavage sites and pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid along with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene plasmid # 141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: ... HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid along with pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro WT) (a gift from Nevan Krogan; Addgene #141370 ...
• Differential Kinetics of SARS-CoV-2 Proteases Revealed by a Dual-Color, BRET-based Protease Biosensor, DuProSense

  Asma Fatima, et al., bioRxiv - Biophysics 2024

  Quote: HEK293T cells were co-transfected with mSca-containing DuProSense biosensor plasmid DNA and either pLVX-EF1alpha-SARS-CoV-2-nsp5-2×Strep-IRES-Puro (Mpro) (a gift from Nevan Krogan; Addgene #141370 ...
• Intermitochondrial signaling regulates the uniform distribution of stationary mitochondria in axons

  Nozomu Matsumoto, et al., bioRxiv - Neuroscience 2020

  Quote: ... 2 μg of tdTomato-Mito-7 (Addgene #58115) (36 ...
• CAR-NK Cells Effectively Target the D614 and G614 SARS-CoV-2-infected Cells

  Minh Ma, et al., bioRxiv - Immunology 2021

  Quote: pcDNA3.1-SARS-CoV-2 Spike (Addgene plasmid #145302) was used to clone SARS-CoV-2 S gene into the SFG backbone using the In-Fusion Cloning kit (Takara Bio) ...
• DMT1 bridges endosomes and mitochondria to modulate mitochondrial iron translocation

  Jonathan Barra, et al., bioRxiv - Cell Biology 2022

  Quote: ... 2 µg of psPAX2 (Addgene, #12260, MA, USA) and 2 µg of lentiCRISPRv2 sgRNA DMT1 ...
• Caenorhabditis elegans junctophilin has tissue-specific functions and regulates neurotransmission with extended-synaptotagmin

  Christopher A. Piggott, et al., bioRxiv - Genetics 2020

  Quote: ... and pCFJ90 Pmyo-2-mCherry (Addgene plasmid 19327) and pCFJ104 Pmyo-3-mCherry (Addgene plasmid 19328)(Frøkjær-Jensen et al. ...
• Limited Dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

  Wei Kan, et al., bioRxiv - Biochemistry 2020

  Quote: ... and combined with pX330A-2-PITCh (Addgene #63670) by golden gate cloning ...
• A large-scale sORF screen identifies putative microproteins and provides insights into their interaction partners, localisation and function

  Dörte Schlesinger, et al., bioRxiv - Molecular Biology 2023

  Quote: ... PITCh sgRNA from pX330S-2-PITCh (Addgene #63670) was cut-out via Eco31I (Thermo Fisher Scientific ...
• In vivo intra-uterine delivery of TAT-fused Cre recombinase and CRISPR/Cas9 editing unveil histopathology of Pten/p53-deficient endometrial cancers

  Raúl Navaridas, et al., bioRxiv - Pathology 2023

  Quote: ... 2 µg of pCA-mTmG plasmid (#26123, Addgene) was added to diluted TAT-Cre and incubated at 37°C for 1 hour ...
• Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

  Jonathan D. Lee, et al., bioRxiv - Microbiology 2023

  Quote: ... and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223 ...
• Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome

  Abby R Kroken, et al., bioRxiv - Microbiology 2023

  Quote: ... and pMD.2 (Didier Trono, Addgene plasmid # 12259), combined with either Cas9 expression vector plenti-Cas9-blast (Feng Zhang ...
• Loss of NAT10 disrupts enhancer organization via p300 mislocalization and suppresses transcription of genes necessary for metastasis progression

  Ruhul Amin, et al., bioRxiv - Cancer Biology 2024

  Quote: ... PITCh sgRNA from pX330S-2-PITCh (Addgene #63670) vector was then inserted into the pX330A-1x2-cNAT10 vector using Golden Gate Assembly (New England Biolab ...
• Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

  Made Harumi Padmaswari, et al., bioRxiv - Bioengineering 2024

  Quote: ... and exon 2-8 was cloned from Addgene #182141 ...
• Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory

  Myung Chung, et al., bioRxiv - Neuroscience 2024

  Quote: ... 85 nl of AAV1-hSyn:Cre (Addgene, 2 × 1013) was injected into vCA1 and 200 nl of a 1:1 cocktail of AAV9-pMeCP2:DIO-Cas9 (2 × 1012 GC/ml ...
• Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory

  Myung Chung, et al., bioRxiv - Neuroscience 2024

  Quote: ... and pAAV2/2 (for AAV2; Addgene plasmid # 104963), pAAV2/5 (for AAV5 ...
• Early steps of multi-receptor viral interactions dissected by high-density, multi-color single molecule mapping in living cells

  Nicolas Mateos, et al., bioRxiv - Biophysics 2023

  Quote: The plasmid pr8ΔEnv.2 was obtained from Addgene, Plasmid #12263) ...
• The FW2.2/CNR protein regulates cell-to-cell communication in tomato by modulating callose deposition at plasmodesmata

  Arthur Beauchet, et al., bioRxiv - Plant Biology 2023

  Quote: ... the AtCas9 (2×35S::AtCAS9-OCST; Addgene #112079) and the linker pICH41780 (Addgene #48019 ...
• 3-nitrotyrosine shortens axons of a non-dopaminergic neuron by inhibiting mitochondrial motility

  Masahiro Hirai, et al., bioRxiv - Neuroscience 2024

  Quote: ... 2 μg of tdTomato-Mito-7 (Addgene_#58115) (Ai et al. ...
• Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes

  Songyuan Zhang, et al., bioRxiv - Plant Biology 2024

  Quote: ... into a level 2 binary vector (Addgene #54346). LRR12-19VvFLS2 fragments carrying computationally predicted polymorphic residue sets (‘comp.Max’ ...
• Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2024

  Quote: ... BCL2L13 Y213A/I216A/W276A/I279A (ΔLIR1+2) (RRID:Addgene_223752), BCL2L13 I224A/L227A/W276A/I279A (ΔLIR1+3 ...
• Advanced Peptide Nanoparticles Enable Robust and Efficient delivery of gene editors across cell types

  Oskar Gustafsson, et al., bioRxiv - Bioengineering 2024

  Quote: ... and Prime editor 2 (herein called PE2) (Addgene plasmids ...