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Citations for Addgene :
551 - 600 of 1340 citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 500 nl of AAV5-EF1a-DIO-ChR2(H134R)-mCherry virus (Addgene #44361-AAV5) was injected unilaterally at 100nl/min ...
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bioRxiv - Neuroscience 2024Quote: ... or AAV8-hSyn-DIO-mCherry (Control virus; 2.3 x 1013 GC/ML, Addgene). Rats in Experiment 3 received a unilateral infusion of AAV8-rCRHp-iCre (200 nL ...
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bioRxiv - Neuroscience 2024Quote: ... The virus is pGP-AAV-syn-jGCaMP8m-WPRE (Addgene 162375, 1.7x1013 GC/mL). The coordinates used were decided according to the mouse brain atlas (Paxinos and Franklin ...
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bioRxiv - Cancer Biology 2021Quote: ... or the third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) were mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Biochemistry 2021Quote: ... The lentivirus envelope and packaging plasmids pMD2.G and pCMV delta R8.2 were gifts from Didier Trono (Addgene plasmids # 12259 and 12263). Plasmids containing human TIM-1 and human TIM-4 were obtained from Origene ...
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bioRxiv - Cell Biology 2021Quote: ... HEK 293 cells were transfected with pLJM1-eGFP or pLJM1-DN-FYN plasmid together with lentivirus packing and envelope plasmids pMD2.G (gift from Didier Trono, Addgene plasmid# 12259) and psPAX2 (gift from Didier Trono ...
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bioRxiv - Genomics 2022Quote: Lentiviral particles were produced by transfecting 293T-17 cells (ATCC: CRL-11268) with the envelope construct pCMV-VSV-G (Addgene plasmid 8454), the packaging construct pCMV-dR8.2 dvpr (Addgene plasmid 8455) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were co-transfected using the PEI method with the lentiviral expression vector and two 2nd generation lentiviral packaging vectors: pMD2.G expressing the VSV-G envelope gene (Addgene plasmid 12259) and pCMVR8.74 expressing the gag/pol genes (Addgene plasmid 22036) ...
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bioRxiv - Cancer Biology 2023Quote: ... third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) was mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Molecular Biology 2024Quote: Lentivirus particles for transduction of IMR90 E6E7 hTERT cells were produced by transfection of an expression plasmid with packaging and envelope plasmids gifted from Didier Trono (Addgene plasmid #12260) and Robert A ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... new lentiviral particles were produced by transfecting HEK293 cells with 4µg Apple-53BP1trunc (69531 from Addgene, RRID:Addgene_69531) + 4µg pMD2.G (12259 from Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... pLKO plasmids were co-transfected into HEK293-T cells along with packaging plasmids pMDLg/pRRE (Addgene #12251), pRSV-Rev (Addgene #12253 ...
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bioRxiv - Microbiology 2019Quote: ... These cytokine-conditioned media were produced from HEK293 cells stably transduced with pAIP-hGMCSFco (Addgene no. 74168) or pAIP-hIL4-co (Addgene no ...
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bioRxiv - Cell Biology 2022Quote: ... we co-transfected HEK293-FT cells with plasmids of interest with VSVG (pMD2.G; Addgene plasmid 12259) and psPAX2 (Addgene plasmid 12260 ...
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bioRxiv - Cancer Biology 2022Quote: ... was purchased from VectorBuilder (Chicago, IL, USA) and the packaging plasmid psPAX2 (#12260) and envelope plasmid pMD2.G (#12259) were obtained from Addgene (Watertown, MA, USA). The plasmid GFP-pLVTHM (#12247 ...
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bioRxiv - Molecular Biology 2024Quote: ... For lentivirus production pCMV-VSV-G (envelope plasmid) and pCMV dR8.2 dvpr (packaging plasmid) were gifts from Bob Weinberg (Addgene # 8454 and #8455, respectively)25 ...
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bioRxiv - Genetics 2021Quote: Mice were injected with Cre-expressing adeno-associated virus AAV1.hSyn.Cre.WPRE.hGH (105553-AAV1, Addgene) and GFP-expressing AAV1.hSyn.eGFP.WPRE.bGH (105539-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: The commercial AAV5-hSynhM3D(Gq)-mCherry or the control virus AAV5-hSyn-mCherry (Addgene) were injected into the sciatic nerve of WT C57BL/6J mice (1μL/sciatic nerve ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transduced with the following virus: pTet-O-NGN2-puro (Addgene #52047): 0.1 μL per 5×104 cells ...
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bioRxiv - Neuroscience 2019Quote: ... A control virus (AAV5-CamKIIα-eGFP, 4.1×1012) (Cat No. 50469-AAV5; Addgene, USA) was administered into the contralateral left EC using identical parameters ...
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bioRxiv - Neuroscience 2022Quote: ... or a control virus AAV9-hSyn-eYFP (Addgene, viral titer 2.8 × 1013 vg/mL) was injected in the NAcSh of WT mice while AAV9-Syn-Flex-GcAMP6f-eYFP (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... The TauRD(P301S)-YFP plasmid DNA with virus packing pDNAs (psPAX2; pMD2.G, Addgene) was transfected into 293T cells by 1mg/mL PEI transfection reagent and incubated for 36 hours ...
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bioRxiv - Neuroscience 2023Quote: Birds were injected with the AAV9-CAG-GCaMP6f-WPRE-SV40 virus (100836-AAV9, Addgene). The total amount of virus injected was 897 nL (65 injections of 13.8 nL each) ...
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bioRxiv - Neuroscience 2023Quote: ... iAChSnFR and iAChSnFR-Null constructs and AAV virus are available from Addgene (137950–137962).
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of a virus encoding AAV5.CamKIIa.GCaMP6f (pENN.AAV.CamKII.GCaMP6f.WPRE.SV40, Addgene viral prep 100834-AAV5) virus was injected unilaterally in the PL (AP +1.9 ...
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bioRxiv - Cancer Biology 2023Quote: ... mitochondria were labelled in cells by transduction with pLV-mitoDsRed virus (Addgene, Plasmid 44386) harvested post expression in 293-FT cells and lentiviral particle production ...
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bioRxiv - Cancer Biology 2024Quote: ... the shRNA constructs were packaged into second-generation virus particles using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Physiology 2024Quote: AAV9 virus expressing jGCaMP8m under a CAG promoter was obtained from Addgene (162381-AAV9). For injections ...
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bioRxiv - Neuroscience 2024Quote: ... Animals used for RSC imaging received a mixture of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (Addgene: 100837), AAV1.cFos-tTA and AAV1.TRE.mCherry at 20 to 120 nL/min into dorsal cortex using stereotactic coordinates −1.7 and −2.3 mm posterior to bregma ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth ...
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bioRxiv - Cancer Biology 2024Quote: HT29 cells previously infected with pLenti-U6-tdTomatato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and TdTomato were used ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg shRNA p53-puromycin (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells grown in twelve 10 cm dishes were co-transfected with active myc-mTOR E2914K mutant (Addgene) and HA-Raptor (Kim et al. ...
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bioRxiv - Cell Biology 2021Quote: Measurement of NFAT4 nuclear translocation in HEK293 cells was achieved by transient overexpression of NFAT4-GFP (Addgene #21664) as previously described(30 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was generated by co-transfection of HEK293 cells with viral vector and packaging plasmids psPAX2 (Addgene 12260) and pMD2.G (Addgene 12259 ...
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bioRxiv - Neuroscience 2022Quote: ... Second generation lentiviral packaging plasmids psPAX and VSV-G envelope expressing plasmid pMD2.G (kind gifts from Didier Trono, Addgene plasmid #12260 and #12259) were used for the virus production ...
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bioRxiv - Genetics 2020Quote: Barcoded MSH2-2A-blR cDNA libraries were packaged into lentivirus by co-transfecting HEK293T/17 cells (ATCC) with the transfer plasmid pool plus envelope and packaging vectors (pMD2.G, Addgene #12259 and psPAX2, #12260). For each pool ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were bilaterally microinjected with 500 nL of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (purchased from Addgene 100837) at 20 -120 nL/min into the dorsal cortex using the stereotactic coordinates ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2020Quote: HT29 cells were infected with the pLenti-U6-tdTomato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and dTomato ...
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bioRxiv - Neuroscience 2021Quote: ... DV 1.4) before injecting 250 nL of virus (pGP-AAV1-syn-jGCaMP7f-SV40-WPRE; Addgene) in 50 nL pulses each interspersed by ∼10 s and the final pulse followed by a 10 min period where the needle was left in place to allow for diffusion ...
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bioRxiv - Neuroscience 2020Quote: ... We then injected the AAV5.CamKII.GCaMP6f.WPRE.SV40 virus (Addgene # 100834; 200 nL at 1 nl.s-1) in hippocampal CA1 using the following coordinates ...
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bioRxiv - Physiology 2020Quote: ... A retrograde AAV virus carrying a Cre gene (AAVrg-hSyn1-Cre, Addgene; 200 nl volume) was injected into the PBN (coordinates ...
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bioRxiv - Cancer Biology 2022Quote: MB-004 cells were transduced with the enti-dCas9-KRAB-T2A-GFP virus (Addgene # 71237). sgRNAs targeting SOX11/HNRNPH1-binding motifs in the distal enhancers were designed using the CHOPCHOP program (https://chopchop.cbu.uib.no) ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5µl of virus (either AAV1-CaMKIIa-ArchT-GFP or AAV8-CaMKIIa-GFP, Addgene, MA) was infused bilaterally with a 1 µl Hamilton syringe into either the PRC (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Anxa1-iCre mice (new line) were injected with AAV1-CAG-FLEX-GCaMP6f virus (RRID: Addgene_100835). For labelling VGlut2+ or Aldh1a1+ dopamine neurons ...
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bioRxiv - Neuroscience 2023Quote: ... the pipette containing AAVPHP.S-CAG-FLEX-tdTomato virus (Addgene 28306-PHP.S, 1.8×1013 vg/mL) targeted at 2 locations in the mandibular branch spaced 250 um apart ...
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bioRxiv - Neuroscience 2023Quote: ... virus expressing tdTomato under the PVIN specific S5E2 enhancer (AAV9-S5E2-tdTomato, Addgene 135630-AAV9) was injected in mice using coordinates ...