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Citations for Addgene :
451 - 500 of 935 citations for Mouse Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Early passage primary MEFs were transformed with SV-40 T antigen containing plasmid pBSSVD2005 (ADDGENE, Cambridge, MA) to generate immortalized MEFs ...
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bioRxiv - Molecular Biology 2022Quote: ... then a fragment of CRY2olig region that was digested from pCRY2olig-mCherry (#60032; Addgene, Watertown, MA, USA) was inserted at the NheI and SmaI sites (pCRY-C220- ...
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bioRxiv - Molecular Biology 2022Quote: Templates for in vitro transcription of SpCas9 guides were amplified from the plasmid pX330 (Addgene, Watertown, MA) using primer pairs with 65 oligonucleotides as a 5’ primer (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... A puromycin resistant gene unit was amplified from plasmid pGL3-U6-sgRNA-PGK-puromycin (Addgene, Watertown, MA) by PCR and the 1473 bp product was then inserted in an EcoRV site in intron 19-20 ...
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bioRxiv - Developmental Biology 2022Quote: Full length inpp5ka (NM_001089493.1) and inpp5kb (XM_021479345.1) cDNAs were cloned into the pCS2+ plasmid (Addgene, Watertown, MA). Digoxygenin-labeled sense and antisense probes were synthesized from the linearized plasmids using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Neuroscience 2023Quote: ... or rAAV5-hsyn-DIO-mCherry (around 5.0 x 1012 vg/ml for each) (Addgene, Watertown, MA, USA) in the dorsal raphe nucleus [Anterior-posterior (AP) ...
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bioRxiv - Neuroscience 2023Quote: ... 400 nl of AAV2/5-CAG-dLight1.1 (1.7×1013 GC/ml, 111067-AAV5, Addgene, Watertown, MA, USA) was slowly injected into the DMS (n = 7 ...
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bioRxiv - Microbiology 2023Quote: ... the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed ...
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bioRxiv - Cancer Biology 2023Quote: ... A CRISPR/dCas9 vector was constructed as follows: pX330A_dCas9-1×2 (Addgene, Watertown, MA; plasmid ID 63596) (20 ...
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bioRxiv - Molecular Biology 2023Quote: The lentiviral shuttle vector coding for mCherry (LV-mCherry) was a from Addgene (#36084, Cambridge, MA, USA). LV-TK ...
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bioRxiv - Neuroscience 2023Quote: ... viral prep #50477-AAV8) or eGFP (AAV8-CaMKIIa-EGFP, Addgene, Cambridge, MA, Addgene, viral prep #50469-AAV8) infusion and unilateral chronic implantation of electrode arrays in both ACC and OFC ...
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bioRxiv - Biophysics 2023Quote: ... pCAGGS-SARS-CoV-2 S D614G (# 156421) and pcDNA3.1 vectors were obtained from Addgene (Watertown, MA, USA), and Invitrogen (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs targeting either MGA or NME1 was cloned in pLKO5.sgRNA.EFS.mCherry or pLKO5.sgRNA.EFS.GFP (Addgene, Watertown, MA), respectively ...
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bioRxiv - Immunology 2023Quote: ... sgRNA targeting Myc was cloned into a Cas9-expressing lentiviral transfer vector (lentiCRISPRv2, # 52961, Addgene, Cambridge, MA) following the methods of the Feng Zhang laboratory (Sanjana et al. ...
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bioRxiv - Neuroscience 2023Quote: 1-4 evenly spaced ∼60 nl injections of the AAV1-synapsin-l-GCamp6f (Addgene, MA stock #100837) that had been diluted to a titer of ∼1×10^12 vg/mL using sterile PBS were made in each cranial window ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV9-CaMKIIa.C1V1(t/t).TS.EYFP (titer: 2.3 × 1013 GC/ml; prepared by Addgene, MA, USA) into the unilateral dorsal hippocampus as described previously 87.
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: CDS of zebrafish fto mRNA was synthesized and inserted into the pCS2+ plasmid (Addgene, Watertown, MA, USA) to get the linearized pCS2+-FTO plasmid for in vivo transcription ...
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bioRxiv - Microbiology 2023Quote: HeLa S3 cells were transduced with a Streptococcus pyogenes (sp)Cas9 expressing lentivirus (#52962; Addgene, Watertown, MA) at an MOI of 20 transduction units (TU)/cell ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred into the destination vectors pDEST-CMV-C-EGFP (#122844; for Lamp1; Addgene, Watertown, MA, USA) or pDest-mCherry-N1 (#31907 ...
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bioRxiv - Neuroscience 2024Quote: ... The dLight virus (700 nL of AAV5-hSyn-dLight 1.2; Addgene, Watertown, MA; Catalog No.111068-AAV5) was injected unilaterally into the NAc core (in mm ...
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bioRxiv - Neuroscience 2023Quote: ... AAV vector encoding for inhibitory DREADD (AAV5-SYN1-hM4Di-HA 1.7*1013 GC/ml (Addgene, Watertown MA)) was injected into the bilateral basolateral amygdala stereotaxically ...
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bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... lentivirus that harbored the mouse GeCKOv2 sgRNA library in the lentiCRISPRv2 vector (Addgene) was produced in HEK293FT cells (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2019Quote: ... GFP-PGC1 plasmid expressing eGFP-tagged mouse PGC1a was acquired from Addgene(50). For in vivo electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... one DATcre mouse was injected with AAV8-hSyn-DIO-mCherry (Addgene, cat#50459) in midbrain (SNc ...
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bioRxiv - Cell Biology 2022Quote: ... human KIFC1(125-673) and mouse BICD2(15-595) were obtained from Addgene (plasmids #133242 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse ULK1 cDNA was isolated from a p3xFLAG-CMV14-mULK1 vector (#24301, Addgene) by PCR and cloned into the Halo-tagged pFN21A vector (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... pCAG_MMP-9 – full length mouse (MMP-9) from pcDNA3.1_MMP-9-HA (Addgene #121172) cloned into pCAG vector ...
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bioRxiv - Genetics 2023Quote: ... Codon-optimized of full-length mouse RNF212B was cloned into pET-28b (+) (Addgene) with a C-terminal 6xHis tag ...
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bioRxiv - Microbiology 2023Quote: The Mouse Genome-wide Knockout CRISPR sgRNA Library was purchased from Addgene (#67988)5 ...
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bioRxiv - Neuroscience 2023Quote: ... pCDNA3-mouse PKA-RIalpha-mEGFP (Addgene plasmid # 45525; http://n2t.net/addgene:45525; RRID:Addgene_45525), pcDNA3-mouse PKA-RIbeta-mEGFP (Addgene plasmid # 45526 ...
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bioRxiv - Neuroscience 2023Quote: ... pcDNA3-mouse PKA-RIbeta-mEGFP (Addgene plasmid # 45526; http://n2t.net/addgene:45526; RRID:Addgene_45526), and pCDNA3-mouse PKA-RIIalpha-mEGFP (Addgene plasmid # 45527 ...
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bioRxiv - Neuroscience 2024Quote: ... and Cdk5rap2 from mouse and human were cloned into FUGW (Addgene plasmid # 14883) [52] ...
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bioRxiv - Microbiology 2020Quote: ... The guides were cloned into the plasmids pX330 or pX548 (obtained from Zhang Laboratory via Addgene; Cambridge, MA) and the PCR products were transcribed with the T7 MEGAshortscript kit (Life Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... Str-KDEL_neomycin was a gift from Franck Perez (Addgene, Watertown, MA, Addgene plasmid # 65306; http://n2t.net/addgene:65306; RRID:Addgene_65306). This construct allowed subsequent labelling of well-fixed ER using fluorescent biotin ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids pHSG-PCNA2 and pHSG-PCNA3 were a gift from Teruyuki Nagamune obtained through Addgene (Cambridge, MA, USA) (Addgene plasmid # 66126; http://n2t.net/addgene:66126; RRID:Addgene_66126) and (Addgene plasmid # 66127 ...
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bioRxiv - Physiology 2020Quote: ... The murine Ntf3 cDNA was cloned into a Rosa26 locus targeting vector 25 purchased from Addgene (Cambridge, MA). Key features of the complete targeting construct as depicted in Suppl ...
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bioRxiv - Neuroscience 2021Quote: ... http://n2t.net/addgene:44361; RRID:Addgene_44361 and viral prep # 44362-AAV2; http://n2t.net/addgene:44362; RRID:Addgene_44362; Addgene, Watertown, MA)47 ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced using 293T cells with the second-generation packaging system psPAX2 (#12260, Addgene, Watertown, MA, USA) and PMD2 ...
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bioRxiv - Cell Biology 2022Quote: ... The vector pSpCas9(BB)-2A-Puro (PX459) V2.0 was provided from Feng Zhang (plasmid 62988; Addgene, Cambridge MA) (Ran et al ...
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bioRxiv - Neuroscience 2021Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA) to drive expression preferentially in principal neurons ...
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bioRxiv - Neuroscience 2021Quote: ... AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (serotype AAV1, viral titer ≥ 5×1012 vg/mL; Addgene, Watertown, MA, USA), pAAV-hSyn-hM4D(Gi)-mCherry (serotype AAV retrograde ...
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bioRxiv - Neuroscience 2020Quote: ... 10 animals received rejections of AAV5-CaMKIIa-hM4Di-mCherry (titer 9.5×10^12 GC/ml; Addgene, MA, USA), while eight animals received injections of a non-DREADD expressing viral control AAV5-CaMKIIa-EGFP (titer 4.3×10^12 GC/ml ...
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bioRxiv - Physiology 2019Quote: ... envelope vector pMD2.G (ID #12259) and scrambled shRNA plasmid (sc: ID1864) were obtained from Addgene (Cambridge, MA). HEK293T cells in 10 cm dishes were transfected using 50µL 0.1% Polyethylenimine ...
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bioRxiv - Immunology 2019Quote: ... Lentivirus was generated by calcium phosphate transient transfection of 293T cells using psPAX2 packaging (Addgene, Cambridge, MA, USA) and pMD2.G (Addgene ...
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bioRxiv - Genomics 2021Quote: ... pAAV-EF1a-Cre was a gift from Karl Deisseroth (Addgene, Watertown, MA; plasmid #55636; http://n2t.net/addgene:55636; RRID:Addgene_55636). First ...
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bioRxiv - Cancer Biology 2020Quote: Expression plasmid encoding N-terminally Flag-tagged hFOXO1-3A (#13508) was purchased from Addgene (Cambridge, MA, pCDNA3 backbone). FOXO1-3A has Ala residue substitutions at Thr-24 ...
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bioRxiv - Genomics 2022Quote: ... 5 μg DNA was incubated with1.75 μg pMD2.G and 3.25 μg pCMV-dR8.91 (Addgene, Watertown, MA; #12259). On the next day ...
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bioRxiv - Neuroscience 2022Quote: ... 100-200 nl virus solution of 3-5 × 1010 genome copies containing AAV5.gfaABC1d.Lck-GCaMP6f (Addgene, MA, USA) were injected at two or three sites in the craniotomy site at the cerebellar cortex (Vermis Lobule 4/5 ...
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bioRxiv - Neuroscience 2022Quote: ... the viral vector pAAV-CamKII-ArchT-GFP (titer: 1.9×1013 genome copies per ml, Addgene, Watertown, MA, USA) was injected bilaterally 0.45 mm from the midline at 1 ...