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Citations for Addgene :
451 - 500 of 1131 citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... a DNA block containing sgEGFP-tRNA-Hnf4a-sg2 was PCR-amplified using pGTR plasmid (Addgene #63143) as a template and primers listed in Table S2 (see also Fig ...
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bioRxiv - Synthetic Biology 2022Quote: ... was cloned by PCR amplifying both the mCherry from LLP469 pEF1a-mCherry-EMPTY-gRNA (Addgene, #100958) and the backbone of αGCN4-p65HSF1-CO ...
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bioRxiv - Molecular Biology 2023Quote: ... full length BRCA1 was PCR-amplified from pCL-MFG-BRCA1 (Addgene #12341 (Ruffner and Verma, 1997)) with a triple Ty1-tag included in the reverse primer and subsenquently cloned into pENTR_1A and transferred to pCW57.1 using gateway cloning ...
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bioRxiv - Neuroscience 2022Quote: ... T2A-QF2 including loxP-flanked 3xP3-RFP was PCR amplified from pBPGUw-HACK-QF2 (Addgene #80276), followed by insertion into pTOPO-acj6 right before the stop codon of acj6 by DNA assembly (New England BioLabs #E2621S) ...
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bioRxiv - Molecular Biology 2022Quote: ... The deaminase and UGI components in ciBE4max and ciAncBE4max were PCR-amplified from pCMV_BE4max (Addgene #112093) and pCMV_AncBE4max (Addgene #112094) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were subsequently cloned into pExpTol2-UAS-E1B-ReaChR-TS-tagRFP-cryaa-mCherry (Addgene #43963) digested with SpeI and PacI using Hi-Fi DNA Assembly.
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bioRxiv - Neuroscience 2023Quote: ... Both PCR products were assembled with the Tol2 Gateway plasmid backbone (pDestTol2pA2-U6:gRNA; Addgene #63157) using Hi-Fi DNA Assembly to obtain elavl3:hArr-TEV;he1.1:YFP.
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-DRD2 was generated by PCR amplifying the DRD2 CDS from DRD2-Tango (Addgene #66269) (introducing a stop codon ...
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bioRxiv - Cancer Biology 2023Quote: ... The mCherry-3X-NLS sequence was PCR amplified from CSII-prEF1a-mCherry-3X-NLS (Addgene #125262). PCR fragments were assembled into pLenti6.3/V5-DEST-GFP
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bioRxiv - Cell Biology 2023Quote: ... Golgi localisation signal (residues 3131 to 3259 of Human Giantin) was extracted by PCR from Addgene’s plasmid #85048 ...
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bioRxiv - Genomics 2023Quote: ... with flanking sequences to enable PCR amplification and Gibson assembly into lentiGuide-Puro (pLG, Addgene #52963). The pooled oligo library was amplified using pLG_U6_foward 5’- GGCTTTATATATCTTGTGGAAAGGACGAAACACCG-3’ and pLG-TRACR_reverse 5’-GACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’ ...
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bioRxiv - Cell Biology 2023Quote: Lysosome localisation signal (residues 27 to 407 of Human LAMP1) was extracted by PCR from Addgene’s plasmid #55308 ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was digested with KpnI and XhoI and integrated into pSH1.6EGFP (Plasmid #42323, Addgene). The Flot1 gene was amplified from genomic DNA from YT16 rice using the primers HindIII_OsFlot1Prom1-F1 and Eco47III_OsFlot-R1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-HTR2A was generated by PCR amplifying the HTR2A CDS from HTR2A-Tango (Addgene #66409) (introducing a stop codon ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-ADRB2 was generated by PCR amplifying the ADRB2 CDS from ADRB2-Tango (Addgene #66220) (introducing a stop codon ...
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bioRxiv - Cell Biology 2023Quote: Keratin sequences were cloned by polymerase chain reaction (PCR) from pBabe-RFP1-KRT5-hygro (Addgene #58493) and pDONR22-KRT6A (DNASU clone HsCD00039474 ...
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bioRxiv - Cell Biology 2023Quote: ... Both pie-1p and pie-1 3’UTR PCR products were amplified using pPK605 plasmid (Addgene) as the template.
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A quantitative characterization of early neuron generation in the developing zebrafish telencephalonbioRxiv - Developmental Biology 2023Quote: ... pCS2-Cre-ERT2 was generated by inserting PCR-generated ERT2 sequence (with pBigTB-CreERT2 (Addgene #149434) as template ...
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bioRxiv - Physiology 2023Quote: ... The PCR product was cloned via AgeI/BamHI into an MCS-BioID2-HA plasmid (Addgene #74224)15 to enable expression of an NCLX-BioID2-HA fusion protein under the control of a CMV promoter ...
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bioRxiv - Developmental Biology 2023Quote: ... flanked by Xba1 sites was generated by touch down PCR using pUAST-Rab35-6myc (Addgene #53503) as template.
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bioRxiv - Cell Biology 2023Quote: The VAPB-Wt expression vector was produced through PCR amplification using mCherry-VAPB (Addgene plasmid – 108126) as the template and the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length and domain of interest were amplified by PCR using emGFP-BAZ1B (#65372; Addgene) as a template and inserted into pEGFP-C2 vector (Takara Bio ...
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bioRxiv - Synthetic Biology 2023Quote: ... Human FUS (residues 1-214) was amplified by PCR using pHR-FUSN-mCh-Cry2WT (Addgene #101223). The IDRs fragments were fused with TPPPCORE domain(aa.45-166 ...
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bioRxiv - Neuroscience 2023Quote: ... oligonucleotide pairs (Extended Data Table 4) were used for PCR and inserted into pCFD5 (Addgene #73914) via Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: ... were cloned by PCR as EcoRI–BamHI fragments into the vector pcDNA3.1 mycBioID52 (Addgene plasmid #35700) in frame with BirA ...
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bioRxiv - Immunology 2024Quote: ... The OVA entry fragment was created using a PCR amplicon from OVA plasmid (Addgene plasmid 64599) with primers containing attB sites (Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the MCP coding sequence was PCR amplified from UBC NLS-HA-2XMCP-tagRFPt vector (Addgene 64541) using MCP F and MCP R primers and inserted in-frame ...
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bioRxiv - Cell Biology 2023Quote: ... The NFAST sequence was amplified by PCR from the Addgene plasmid pAG148 FRB-NFAST (Addgene #130812) and ligated at the 3’ of ER-targeting sequence into pcDNA3.
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was further digested using BamHI along with tol2kit 101_p5E-Ubiquitin plasmid (Addgene #27320). Both were purified and 101_p5E-Ubiquitin was dephosphorylated using NEB Antarctic phosphatase following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was PCR-amplified from wt-TDP43- tdTomato-HA plasmid (Addgene 28205, a gift from Zuoshang Xu)63 and cloned in frame with the N-terminal 6- His-SUMO-TEV-site into the SspI site of pET 6His-SUMO-TEV LIC (1S ...
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids for WT and catalytic mutant mouse Lipin-1 were obtained from Addgene. Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: The truncated mouse Fzd2 gene (Fzd2-delN) was cloned from pRK5-mFzd2 (#42254, Addgene) with a 1D4 tag at the C terminus by PCR using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... GFP and gRNAs against mouse Ampkα1 (79004) and Ampkα2 (79005) were obtained from Addgene. C2C12 cells were first transfected with Ampkα1 gRNA and sorted with GFP ...
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bioRxiv - Cell Biology 2020Quote: Mouse GeCKOv2 CRISPR knockout pooled library was a gift from Feng Zhang (Addgene # 1000000052). To prepare lentivirus particles ...
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bioRxiv - Neuroscience 2023Quote: ... and pCDNA3-mouse PKA-RIIalpha-mEGFP (Addgene plasmid # 45527; http://n2t.net/addgene:45527; RRID:Addgene_45527) were gifts from Haining Zhong 73 ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... A cDNA for GFP-tagged mouse septin 6 was obtained from Addgene (plasmid #38296) and cloned into the pLVX-IRES-puro vector (Clontech) ...
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bioRxiv - Bioengineering 2021Quote: ... The GoldenPiCS Kit was a gift from the Gasser/Mattanovich/Sauer group (Addgene kit #1000000133). All coding sequences were amplified with high-fidelity Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
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bioRxiv - Developmental Biology 2023Quote: TALEN plasmids were constructed using the Platinum Gate TALEN Kit (Kit #1000000043, Addgene, Cambridge, MA) as previously described (Sakuma et al. ...
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bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
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bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli codon optimized dSpyCas9 was PCR amplified from vector pdCas9 (gift from Luciano Marraffini, Addgene plasmid #46569) (43 ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PCR product was subsequently cloned in the E1b-GFP-Tol2-Gateway construct (Addgene #37846; (71) by Gateway cloning ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was cloned into the BGlII/EcoR1 sites of the AAV-hSyn-EGFP (Addgene, 114213), upstream of the human Synapsin 1 promoter ...
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bioRxiv - Genomics 2020Quote: ... The ORI minimal promoter and flanking region was PCR amplified from the hSTARR-seq plasmid (Addgene #99296) with a custom primer that included homology for Gibson cloning and KAPA HiFi HotStart ReadyMix (Kapa KK2602 ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Neuroscience 2021Quote: ... The GFP coding region containing artificial introns was PCR-amplified from the vector pPD95.69 (Addgene plasmid #1491) using primers to add segments encoding SGGGGS and SGGGTS to flank the N- and C-termini of GFP ...
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bioRxiv - Cancer Biology 2021Quote: ... the tetracycline inducible expression construct was PCR-amplified from pCW57.1 (a gift from David Root, Addgene # 41393) and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs ...
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bioRxiv - Cell Biology 2021Quote: ... a wild copy of the TYR gene was PCR amplified form the pEGFP-TYR plasmid (Addgene, 32781) with NheI and AgeI overhangs ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified with PCR and cloned into Ac5-Stable2-neo (Rosa Barrio and James Sutherland60, Addgene 32426) with inserts removed by PCR.