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Citations for Addgene :
451 - 500 of 1249 citations for Mouse Anti Hepatitis B Virus X Protein Antibody 1884 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... For DREADD experiments 6-7 week old mice were injected bilaterally with the inhibitory virus AAV-hSyn-hM4D(Gi)-mCherry (AddGene, 50475-AAV2) or control virus AAV-hSyn-EYFP (AddGene ...
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bioRxiv - Neuroscience 2023Quote: ... or left IC (0.9 mm caudal and 1 mm lateral from lambda suture) to inject 100-200 nL of pAAV-Syn-Chronos-GFP virus (Addgene #59170-AAV1). At the end of the surgery ...
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bioRxiv - Cancer Biology 2023Quote: ... or pLenti-PGK-tdTomato-Akaluc (TdT-AkaLuc) virus (subcloned from pLenti-PGK-Venus-Akaluc(neo)) and HR180-LGR5-iCT plasmids through Gibson assembly (Addgene 124701; 129094). For cecal injections ...
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bioRxiv - Neuroscience 2023Quote: ... Two small holes were made in the skull above the injection sites and 200nl (100nl/min) of AAV8-hSyn-DIO-mCherry or AAV8- hSyn-DIO-hm4Di-mCherry virus (Addgene, Watertown, MA) was infused in both hemispheres of the PVN using a 1µL Hamilton syringe (0° angle ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth, Addgene, Watertown, MA) into the LC ...
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bioRxiv - Molecular Biology 2021Quote: ... The reference sgRNA sequences for human GeCKO v2.0 (A and B) were downloaded from Addgene (https://www.addgene.org/pooled-library/) ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV8-hSyn-DIO-hM4D(Gi)-mCherry (gift from B. Roth; Addgene viral prep # 44362-AAV8), pAAV8-hSyn-DIO-hM3D(Gq)-mCherry (gift from B ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV8-hSyn-DIO-hM3D(Gq)-mCherry (gift from B. Roth; Addgene viral prep 44361-AAV8), AAV8-Ef1a-DIO-mCherry (gift from B ...
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bioRxiv - Cancer Biology 2021Quote: PDXO cells were transduced with pHIV-Luc-ZsGreen (a gift from B. Welm; Addgene# 39196), pLKO.1-Scr-TD or pLKO.1-Cys-TD as previously described (Tavora et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The original CIRTs-6: B-defensin 3-TBP6.7-YTHDF2 plasmid (# 132544) was purchased from Addgene and the YTHDF2 domain was PCR amplified and cloned into the AgeI and NheI sites ...
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bioRxiv - Molecular Biology 2023Quote: ... The type I-B Anabaena variabilis ATCC 29413 CAST was sub-cloned from Addgene (#168137) [16] ...
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bioRxiv - Microbiology 2023Quote: ... this plasmid was used in a Golden Gate reaction using pMB1-B (Addgene number 190115) and BsaI-HF (NEB ...
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bioRxiv - Cell Biology 2022Quote: A His-tagged mouse Capping Protein (F-actin-capping protein subunit alpha-1 and F-actin-capping protein subunit beta) plasmid was obtained from Addgene as described above (Plasmid #89950) ...
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bioRxiv - Neuroscience 2023Quote: ... AAV-DJ-EF1a-Coff/Fon-GCaMP6m (4.5 x e12, Addgene plasmid) was packaged at the HHMI Janelia Research Campus virus facility ...
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bioRxiv - Neuroscience 2023Quote: ... or mixed with AAV5.hSynap.FLEX.SF-iGluSnFR.A184S (Addgene, titer 1.3 x 1013). For GCaMP expression in the DRG neurons and their axonal terminals within the DCN ...
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bioRxiv - Neuroscience 2023Quote: ... or AAVrg-hSyn-Cre (≥ 1.8 x 1013 vg/ml, Addgene # 105553) virus was bilaterally infused onto the iCA1 (AP-2.75 ...
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bioRxiv - Neuroscience 2021Quote: Pyramidal cell imaging experiments were performed by injecting a recombinant adeno-associated virus (rAAV) encoding GCaMP6f (rAAV1-Syn-GCaMP6f-WPRE-SV40, Addgene/Penn Vector Core) into male wild-type mice.
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bioRxiv - Neuroscience 2020Quote: ... A small (~0.5 mm) craniotomy was made and the virus AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6s.WPRSE.SV40 (Addgene 50942, titer 1.9 × 1013 vg/ml, −50 nL) was injected into the hippocampus (from bregma ...
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bioRxiv - Neuroscience 2022Quote: ... one group of animals (n=6) received a bilateral injection of a virus driving expression of the calcium indicator GCaMP7s (AAV9-hSyn-GCaMP7s-WPRE; Addgene; 300nL per side) targeting the CA1v at the following stereotaxic coordinates ...
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bioRxiv - Neuroscience 2022Quote: ... ACS-4500) using Lipofectamine 2000 in FBS free Optimem together with the virus packaging plasmids (psPAX2 and pMD2-G, Addgene # 12260 and # 12259) and the plasmid expressing either wild-type U4atac or an Empty Vector ...
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bioRxiv - Developmental Biology 2023Quote: ... a total of 9.45 μg of each library plasmids combined with 6.75 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmids 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Genetics 2023Quote: ... a total of 13.6 μg core enhancer perturbation library plasmids with 5.44 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmid 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Neuroscience 2022Quote: ... mice were first injected with a helper virus (AAV5- hSyn-FLEX-splitTVA-2A-mCherry-2A-B19G; produced in-house, modified after Addgene plasmid no. 52473) into MEC ...
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bioRxiv - Neuroscience 2023Quote: ... typical dose 6.0×108 gc/mL) and a reporter virus (AAV-PHP-eB_7x-TRE-tdTomato, typical dose 1.8×1011 gc/mL) (Addgene plasmid id: #191210 and, #191207). The viral titers of the tTA virus used were empirically adjusted based on the Cre driver line to yield sparsely labeled brains ...
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bioRxiv - Neuroscience 2024Quote: ... DREADD+ group) was bilaterally injected in the dDG with pAAV5-CaMKIIa-hM4D(Gi)-mCherry (virus titer ≥ 3×1012 vg/ml, Addgene, North Carolina, USA). Inhibitory DREADDs are activated by the artificial ligand clozapine-N-oxide (CNO ...
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bioRxiv - Developmental Biology 2021Quote: ... The reference sgRNA library sequences for human GeCKO v2.0 (A and B) were downloaded from Addgene (https://www.addgene.org/pooled-library/) ...
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bioRxiv - Cancer Biology 2020Quote: Cas9-expressing p185+ B-ALL cells were transduced with the Brie CRISPR KO library (Addgene #73633) at a multiplicity of infection of 0.5 with an sgRNA coverage of 400x (24) ...
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bioRxiv - Neuroscience 2022Quote: Ankyrin-G-190-GFP (plasmid #31059) and Ankyrin-B-2XHA (plasmid #31057) were bought from Addgene. The 3XHA-Ankyrin-G domain constructs were gifts from Dr ...
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bioRxiv - Plant Biology 2019Quote: ... a BP reaction was performed with pDONR 221 and pMDC123SB-AtMIR390a-B/c28 (Addgene ID: 51775). pMDC123SB-AtMIR390a-B/c contains AtMIR390a 5’ end and AtMIR390a 3’ end which were split by Bsa I-flanking ccdB expression modules ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with Protein A/G-MNase fusion protein (Addgene, Cat #123461) at 700 ng/mL for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse TRIM21 (Addgene #105516) and CRY2Clust (Park et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse Ebf1 cDNA (Addgene) was cloned into pLVX Tet-One Puro plasmid (Clontech) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mouse Gqα15 (Addgene #40753) (Offermanns and Simon ...
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bioRxiv - Neuroscience 2022Quote: ... Err2VP16 was generated by fusing the open reading frame for the herpes simplex virus-1 (HSV-1) VP16 (amplified from pActPL-VP16AD plasmid, Addgene plasmid #15305, Watertown, USA) activation domain to Err2 ...
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bioRxiv - Neuroscience 2023Quote: We performed an initial calibrating analysis on Emx-Cre mice (see Animals) expressing Cre-dependent GFP virus (Addgene pCAG-FLEX-EGFP-WPRE; #51502-AAV9) following a dot-wise application of 1:2000 diluted virus (see Organotypic Slice Culture methods) ...
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bioRxiv - Bioengineering 2023Quote: ... was transfected with 1 µL AAV1-hSyn1-GCaMP6f-P2A-nls-dTomato virus (Addgene viral prep #51085-AAV1, http://n2t.net/addgene:51085, RRID:Addgene 51085, a gift from Jonathan Ting) diluted 1 ...
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bioRxiv - Neuroscience 2022Quote: ... two 200 nL injections of AAV.CAG.Flex.GCaMP6s.WPRE.SV40 (1.9 x 1013 GC/ml, Addgene), were performed in the LC of C57BL/6-Tg(Dbh-icre)1Gsc mice (Jax 4355551 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV5-hSyn-hM4D(Gi)-mCherry (8.6 x 1012 vg/mL, Addgene #50475), and AAV5-hSyn-hM3D(Gq)-mCherry (1.9 x 1013 vg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... rAAV5-hsyn-mCherry-WPRE (Addgene #114472, Titer: 8 x 1012 GC/mL) was injected unilaterally (60-100 nL at 40 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... AAV8-hSynapsin1-hM3D(Gq)-mCherry (Addgene, #50474, ≥ 2 x 1012 vg/ml) or AAV9-hSynapsin1-Lamp1-mScarlet-I (3.06 x1012 vg/ml ...
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bioRxiv - Immunology 2020Quote: ... Constructs for MRTFA/B silencing and overexpression were gifts from Ron Prywes (Addgene # 27161, #19846, and #27175). To generate inducible expression constructs ...
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bioRxiv - Neuroscience 2021Quote: ... AAV9-Ef1a-DO-hChR2(H134R)-mCherry (gift from B. Sabatini; Addgene plasmid 37082(Saunders et al., 2012); Vigene Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... we generated pAAV-GFAP-GCaMP6m plasmid from flexed-GCaMP6 and pZac2.1-GfaABC1D-mCherry−hPMCA2w/b (Addgene, 111568) and used AAV2/9-pAAV-GFAPGCaMP6m at a concentration of 1.07527E+13 genome copies per ml (gc/ml) ...
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bioRxiv - Immunology 2021Quote: ... green fluorescence protein (GFP) or an enhanced yellow fluorescent protein (EYFP) sequence (pcDNA1.3-, Addgene) using standard cloning methods ...
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bioRxiv - Genetics 2019Quote: ... mCherry fluorescent protein marker (Addgene), and ampicillin resistance gene ...
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bioRxiv - Neuroscience 2022Quote: ... Each mouse received bilateral injections (15 nl/ hemisphere at a rate of 15 nl/min) of either excitatory DREADD virus pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene Cat# 50476-AAV5, titer: 1.7x1013) or control virus pAAV-CaMKIIa-EGFP (Addgene Cat# 50469-AAV5 ...
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bioRxiv - Systems Biology 2020Quote: ... we indirectly titered each experimental replicate’s batch of virus by co-transducing parallel samples of HCFs with pMXs-DsRed Express (Addgene 22724; a gift from Shinya Yamanaka) and pMXs-TurboGFP (see “Cloning” above ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21(DE3) RIL from the pET-derived vector 14-B (a gift from S. Gradia; Addgene 48308) in LB medium individually ...
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bioRxiv - Systems Biology 2020Quote: HEK293T cells were transfected with the combined A and B components of the GeCKO v2 (Addgene #1000000048, #1000000049) whole genome library (123,411 sgRNAs in total ...
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bioRxiv - Neuroscience 2022Quote: ... the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568) with primers designed to incorporate a N-terminal HA tag encoding the amino acids YPYDVPDYA and used to replace mCherry-PMCA2w/b in the same backbone at the 5’ NheI and 3’ XbaI restriction sites using the NEBuilder Hifi DNA assembly kit ...