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Citations for Addgene :
451 - 500 of 1416 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and lentiviral particles from pLenti6.3/TO/V5 containing IDH1R132H,13 or pLentiCRISPRv2 (Addgene #52961) were produced in 293T cells as previously described18 ...
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bioRxiv - Cell Biology 2021Quote: ... RAW264.7 were infected with lentiviral particles obtained from EB1-GFP lentiviral vector (Addgene 118084) for 5 hours in 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral particles were produced in HEK-293T cells with the vectors psPAX2 (12259, Addgene), pMD2.G (12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmids were packaged into Lentiviral particles using the 2rd generation packaging plasmid (Addgene). 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: ... to inject 200–400 nl of AAV9-Flex-ChR2-tdTomato (~1011 viral particles; Addgene) 250-400 μm below the cortical surface (50 nl/min) ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral particles were produced by co-transfecting the envelope plasmid pCMV-VSV-G (Addgene), packaging plasmid pCMV-dR8.2 dvpr (Addgene) ...
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bioRxiv - Microbiology 2020Quote: Lentiviral vector particles were produced by co-transfection of packaging plasmid psPAX2 (Addgene #12260), the respective lentiviral transfer vector pWPI ...
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bioRxiv - Neuroscience 2023Quote: ... Cre-dependent pAAV-FLEX-tdTomato (2.1×1013 particles/ml; Addgene viral prep #28306-AAVrg) was injected into the same hemisphere at 0.7 mm anterior to Bregma ...
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bioRxiv - Developmental Biology 2023Quote: ... Lentiviral particles were generated by transient co-transfection with the pMD2.G (12259, Addgene) and psPAX2 (12260 ...
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bioRxiv - Cancer Biology 2024Quote: ... the shRNA constructs were packaged into second-generation virus particles using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Biophysics 2021Quote: ... mEos2 (obtained from Davidson Lab) was cloned into a CMV-MCP-YFP plasmid (from Mazhar Adli from Addgene) to replace MCP-YFP21 ...
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bioRxiv - Developmental Biology 2021Quote: ... the sequence of mCherry-T2A-(Puromycin-resistance) was retrieved from pCDH-CMV-mCherry-T2A-Puro (Addgene plasmid # 72264) and was cloned into pUC19 at the HindIII cleavage site using HiFi assembly (NEB ...
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bioRxiv - Neuroscience 2022Quote: AAV expressing Cre-recombinase under the control of the CMV promoter (gift from Fred Gage; Addgene plasmid #49056).
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bioRxiv - Cell Biology 2022Quote: ... and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3 (gift from Eric Campau (Addgene plasmid # 26429) to infect AoSMC cells as described in (Kubben et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ; http://n2t.net/addgene:122848; RRID:Addgene_122848) (94 ...
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bioRxiv - Systems Biology 2021Quote: ... Both color variants of PKAR-FKBP-FP were cloned into pLenti CMV puro destination vectors (Addgene plasmid #17452). Lyn-FRB was cloned into the pLenti CMV blast destination vector (Addgene plasmid #17451) ...
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bioRxiv - Genomics 2020Quote: ... pBS-L1PA1-CH-mneo (CMV-LINE-1) was a gift from Astrid Roy-Engel (Addgene plasmid # 51288; http://n2t.net/addgene:51288; RRID:Addgene_51288)42 ...
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bioRxiv - Cell Biology 2021Quote: ... The FIRE-pHLy expression cassette was cloned into lentiviral vectors with CMV promoter (pLJM1-EGFP; Addgene Plasmid #19319) and hUbC promoter (FUGW ...
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bioRxiv - Cancer Biology 2020Quote: ... and an envelope plasmid (CMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454; http://n2t.net/addgene:8454; RRID:Addgene_8454), [124] ...
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bioRxiv - Cell Biology 2021Quote: ... and mitochondrial (CMV-mito-CAR-GECO1) targeted calcium biosensors were gifts from Robert Campbell (Addgene #61244, and #46022) 44 ...
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bioRxiv - Biophysics 2020Quote: We amplified OsTIR1 from pMK232 100 via PCR with the primers Cloning_OsTIR1_fw and Cloning_OsTIR1_rev and inserted it into the pLenti backbone from pLenti-CMV-rtTA3 (Addgene plasmid #26429) by digestion with BstXI ...
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bioRxiv - Biochemistry 2022Quote: ... or the pLenti CMV Puro DEST vector (a gift from Eric Campeau and Paul Kaufman, Addgene plasmid #17452) by an LR clonase II reaction (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... which was then used as a donor to transfer mitoLbNOX into pLenti-CMV-Hygro-DEST (w117-1) (Addgene, 17454 a gift from Eric Campeau & Paul Kaufman ...
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bioRxiv - Neuroscience 2022Quote: ... we co-electroporated animals at stage 46-47 with pGP-CMV-GCaMP6f (2 mg/mL, Addgene plasmid # 40755) and CMV-turboRFP (1mg/ml) ...
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bioRxiv - Biochemistry 2022Quote: HEK293T cells were transiently transfected with pGP-CMV-GcAMP6s (Ca2+ Sensor plasmid, Addgene, Cat. no. 40753; 4 µg), 5HT2c receptor (as positive control ...
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bioRxiv - Immunology 2022Quote: ... New Jersey) and cloned into the NotI and EcoRV restriction sites in the pShuttle-CMV plasmid (Addgene, 16403). The resulting pAF300 plasmid was linearized with PmeI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... DIV 4 neurons were transfected with pGP-CMV-GCaMP6f (a gift from Douglas Kim, Addgene plasmid # 40755; http://n2t.net/addgene:40755 ; RRID:Addgene_40755) (87 ...
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bioRxiv - Cell Biology 2021Quote: ... and subcloned into pLenti-CMV-GFP-Hygro (656-4) (gifted from Eric Campeau & Paul Kaufman; Addgene plasmid # 17446) using NEBuilder® HiFi DNA Assembly Kit (NEB ...
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bioRxiv - Microbiology 2021Quote: ... and pLenti CMV GFP Neo (657-2) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17447). Expression plasmids SARS2-S2’-AA ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear (CMV-NLS-R-GECO) targeted calcium biosensors were gifts from Robert Campbell (Addgene #61244, and 32462) [40,41] ...
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bioRxiv - Immunology 2020Quote: ... The vector pEQ276 (encoding CMV IE1 and 2 genes) was a gift from Adam Geballe (Addgene plasmid #83945)67 ...
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bioRxiv - Neuroscience 2021Quote: ... AAV5-CMV-EGFP (Suppl. Figs. 1A, 6 and 7D; Salk Vector Core; 2.08 x 1012; Addgene plasmid #32395); AAV9-FLEX-rev-ChR2-tdTomato (Suppl ...
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bioRxiv - Cell Biology 2020Quote: ... pLenti CMV hygro DEST (a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17454; http://n2t.net/addgene:17454; RRID:Addgene_17454) or pLenti-mCherry-GWT (a kind gift from F ...
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bioRxiv - Neuroscience 2019Quote: CAG-Mito-R-GECO: CMV-Mito-R-GECO1 (a gift from Robert Campbell; Addgene plasmid # 46021, RRID:Addgene_46021 (74)) was digested PmeI and the mito-R-Geco fragment was subcloned into EcoRV-digested pBSKII SK+ (Stratagene) ...
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bioRxiv - Cell Biology 2020Quote: ... Linearized pShuttle-CMV plasmids were transformed into the final viral backbone using electrocompetent AdEasier-1 cells (Addgene, #16399). Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: The Tg(fli1a:Brainbow1.0L)mu254 (flibow) transgenic fish was generated by cloning the CMV-Brainbow-1.0 L construct (Addgene #18721) downstream of the fli1a promoter62 ...
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bioRxiv - Biochemistry 2022Quote: ... The pGP-CMV-jGCaMP7s was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 104463; http://n2t.net/addgene:104463; RRID:Addgene_104463) (Dana et al ...
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bioRxiv - Cell Biology 2022Quote: ... The PA-mCherry sequence was cloned into pShuttle-CMV plasmid (gift from Dr. Bert Vogelstein – Addgene plasmid # 16403; http://n2t.net/addgene:16403; RRID:Addgene_16403). An adenovirus construct was generated using the pAdeasy system[55].
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bioRxiv - Neuroscience 2023Quote: ... The CMV::tdTomato-Lifeact-7 plasmid (abbreviated Lifeact throughout the manuscript) was a gift from Michael Davidson (Addgene plasmid # 54528 ...
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bioRxiv - Molecular Biology 2023Quote: ... pLVpuro-CMV-N-EGFP was a gift from Robin Ketteler (Addgene plasmid # 122848; http://n2t.net/addgene:122848 ; RRID:Addgene_122848). pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Cell Biology 2023Quote: ... and cloned into the CMV backbone vector prepared by linearizing the mEGFP-N1-YAPS127A-L318E plasmid (Addgene, #166465) using NheI-HF and BsrGI-HF.
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bioRxiv - Bioengineering 2023Quote: ... pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA was a gift from Feng Zhang (Addgene plasmid # 61591 ...
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bioRxiv - Cancer Biology 2023Quote: ... pCDH-EF1-copGFP-T2A-Puro and pCDH-CMV-mCherry-T2A-Puro were gifts from Kazuhiro Oka (Addgene plasmid # 72263 ; http://n2t.net/addgene:72263 ; RRID:Addgene_72263).
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bioRxiv - Cancer Biology 2023Quote: ... using pLenti_mENPP1_fwd and pLenti_mENPP1_rev primers in Table S1 and inserted into the XbaI-BamHI sites of pLenti-CMV-GFP-Puro (Addgene). To clone pLenti-CMV-mENPP1-T238A-GFP-Puro plasmid ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transiently transfected with the genetically encoded Ca2+ indicator pGP-CMV-GCaMP6f (a gift from Douglas Kim & GENIE Project; Addgene plasmid # 40755; http://n2t.net/addgene:40755; RRID:Addgene_40755), (Chen et al. ...
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bioRxiv - Bioengineering 2023Quote: ... and fluorescent HMEC1s were created by introducing mCherry (pCDH-CMV-mCherry-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 72264; http://n2t.net/addgene:72264; RRID:Addgene_72264) plasmids ...
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bioRxiv - Biochemistry 2023Quote: WT S100A1 and its variants were synthesized (Eurofins MWG) and cloned into the pAdTrack-CMV plasmid (#16405, Addgene) (Table S1) ...
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bioRxiv - Neuroscience 2024Quote: ... This vector was generated using the plasmids pPRIME-CMV-GFP-FF3(34) (a gift from Stephen Elledge (Addgene plasmid # 11663; http://n2t.net/addgene:11663; RRID:Addgene_11663)) and pAAV-PTRE-tight-hM3Dg-mCherry(35 ...
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bioRxiv - Neuroscience 2024Quote: ... This vector was generated using the plasmids pPRIME-CMV-GFP-FF3(34) (a gift from Stephen Elledge (Addgene plasmid # 11663 ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 ligation as previously described 18• Plasmids encoding full-length BRAF wildtype (p3xFLAG-CMV-BRAF, Addgene #131710) and V600E mutant (p3xFLAG-CMV-BRAF-V600E ...