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No products found
because this supplier's products are not listed.
Francisco J. Calero-Cuenca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Reverse transcription quantitative PCR (RT-qPCR) was performed using Power SYBR Green PCR MasterMix (Alfagene) according to the manufacturer’s instructions and using primers forward and reverse at 0,25 μM (final concentration ...
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No products found
because this supplier's products are not listed.
Sandra L. Bixler, et al.,
bioRxiv - Microbiology 2021
Quote:
Total SARS-CoV-2 E gene and subgenomic E gene target copy numbers were determined by real-time RT-PCR using previously described assays (24–25) and a synthetic RNA containing the subgenomic E RNA sequence (Bio-Synthesis, Inc., Lewisville, TX, USA). Extracted nucleic acid was tested in triplicate (5 μL extracted nucleic acid ...
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No products found
because this supplier's products are not listed.
Michelle Zuo, et al.,
bioRxiv - Immunology 2021
Quote:
... incubated with diluted mouse serum (1:8 or 1:16 dilution) and biotinylated detection antibodies (mAB2:1, UmanDiagnostics). Upon adding streptavidin-conjugated β-galactosidase (Quanterix) ...
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No products found
because this supplier's products are not listed.
Wei-Ping Hu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 16 PD0325901 (M1763, Abmole, USA) was the inhibitor of MEK pathway and was dissolved in dimethyl sulfoxide (DMSO ...
-
No products found
because this supplier's products are not listed.
Jinze Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
Blood flow in the mouse brain was recorded in real-time using an RFLSI III device (RWD Life Sciences, Shenzhen, China) throughout the whole process of ischemic stroke ...
-
No products found
because this supplier's products are not listed.
Arka Sen Chaudhuri, et al.,
bioRxiv - Immunology 2022
Quote:
... or Foxp3 (clone FLK-16; Nordic Biosite). Cell surface expression of the receptors RAGE and TLR4 was determined by staining the cells with anti-RAGE and anti-TLR4 or their respective isotype control antibodies ...
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No products found
because this supplier's products are not listed.
Cassandra Velasco, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... PCR master mixes were decontaminated with double-stranded DNAse treatment (PCR decontamination kit, Arcticzymes, Tromsø, Norway). Sterile water was processed using the same procedure as a negative control ...
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No products found
because this supplier's products are not listed.
Kyra Swanson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... A single horizontally placed spout (5/16” sipper tube: Ancare) was mounted to a lickometer (MedAssociates ...
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No products found
because this supplier's products are not listed.
Kevin G. Burt, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and 20 ng/mL IFNγ (Shenandoah Biotech, Cat #200-16) in complete macrophage media ...
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The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically...
Cat# B40015, SKU# B40015-500rxns,
500rxns, $267.00
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B. van de Kooij, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Medium containing Olaparib (16 nM or 50 nM) (Selleck Chemicals, Planegg, Germany), doxycycline (1 μg/mL ...
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No products found
because this supplier's products are not listed.
Malin C. Erlandsson, et al.,
bioRxiv - Immunology 2021
Quote:
... IFNγ (PelikineM1933, detection limit 3pg/ml, Sanquin, Amsterdam, the Netherlands), IL10 (DY217B ...
-
No products found
because this supplier's products are not listed.
Marshall Lukacs, et al.,
bioRxiv - Genetics 2019
Quote:
... was used for detection on an Alliance chemiluminescence imaging system (UVITEC Cambridge).
-
No products found
because this supplier's products are not listed.
Qi Wang, et al.,
bioRxiv - Genomics 2023
Quote:
... and RNasin Plus (Promega)] 10-15 times using pestle A “loose” followed by pestle B “tight” 10-15 times (DWK Life Sciences, Millville, NJ, USA). Homogenate was passed through a 70 µm 1.5 ml mini strainer (PluriSelect ...
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No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by adding the M13-HRP-labeled detection antibody (Progen, 1:2000 Dilution) for 1 h ...
-
No products found
because this supplier's products are not listed.
Ekaterina Buzun, et al.,
bioRxiv - Microbiology 2023
Quote:
... for 16 h at 37 °C under anaerobic conditions (10 % H2, 10 % CO2, 80 % N2; Coy Lab Products). For growths utilizing a sole carbon source ...
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No products found
because this supplier's products are not listed.
Thomas HB FitzGerald, et al.,
bioRxiv - Neuroscience 2019
Quote:
... agents update beliefs about all states within a fixed-length time window that includes the present time but stretches a set distance into the past (Figure 1) (FitzGerald et al., 2017). This window moves forward in time at the same rate that observations are gathered ...
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No products found
because this supplier's products are not listed.
Lizhi He, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... lysed by grinding 50 times in Wheaton A (Wheaton, 357538), and then incubated on ice for 20 minutes ...
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No products found
because this supplier's products are not listed.
Dahlia Rohm, et al.,
bioRxiv - Genomics 2024
Quote:
... for 16-48 hours when cells were passaged with Accutase (StemCell Tech, 07920; Innovative Cell Technologies, AT104; Gibco A1110501) and otherwise omitted.
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No products found
because this supplier's products are not listed.
Samuel R. Witus, et al.,
bioRxiv - Biochemistry 2023
Quote:
... cells were shifted from 37 °C to 16 °C and supplemented with 1 mM Bpa (dissolved in 1M NaOH; Bachem) and 100 µM ZnCl2 ...
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No products found
because this supplier's products are not listed.
Michelle E. Maxson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Time series images were analyzed using Volocity v6.3 software (Quorum Technologies), and SidK-AL568 molecule fluorescence over time plotted to generate traces where the number of steps required for SidK-AL568 spot disappearance were counted ...
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No products found
because this supplier's products are not listed.
Thibault Courtellemont, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were passed one time through a French press (Constant Systems LTD) at 4°C with 2.2 kpsi of pressure ...
-
No products found
because this supplier's products are not listed.
Roman Franěk, et al.,
bioRxiv - Zoology 2019
Quote:
... and 3 μl PCR H2O (Top-Bio). Reaction conditions were 30 cycles of 94 °C for 30 s ...
-
No products found
because this supplier's products are not listed.
Johanna F. Dekkers, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Adult female NSG mice (15-16 weeks old) received sub-lethal total body irradiation (1,75 Gy) and subcutaneous implantation of an β-estradiol pellet (Innovative Research of America) on Day −1 ...
-
No products found
because this supplier's products are not listed.
Ronald Rodriguez, et al.,
bioRxiv - Microbiology 2022
Quote:
... The Tn library was diluted to an OD600 of 0.01 (∼ 5 × 106 CFU/ml) in 10 ml of 7H9 with and without ETH (TCI Chemicals, 16 μg/ml), CLR (Ambeed ...
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No products found
Ana Carreras Mascaro, et al.,
bioRxiv - Neuroscience 2023
Quote:
... fluorescently labelled EVs were immobilised in a non-denaturing polyacrylamide gel (16% w/w acrylamide/bis-acrylamide) in 96-wells glass-bottomed plates (Cellvis, P96-0-N). Per sample ...
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No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated for 1.5 hours at RT with the following primary antibodies: LBR (1:200, Atlas Antibodies HPA062236), LaminB1 (1:500 ...
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No products found
because this supplier's products are not listed.
Noah J Harris, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Vesicles were extruded 11 times through a 150nm NanoSizer Liposome Extruder (T&T Scientific) and stored at −80°C.
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No products found
because this supplier's products are not listed.
Rose Z. Hill, et al.,
bioRxiv - Physiology 2023
Quote:
Mice were lightly scruffed one at a time over sterile hydrophobic LabSand (Braintree Scientific) until they urinated and for no more than 30 sec ...
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No products found
because this supplier's products are not listed.
Rémi Porte, et al.,
bioRxiv - Immunology 2024
Quote:
... brain cell suspensions were incubated for 45 min at RT with the following barcoded dextramers diluted in complete RPMI: Kb-SIINFEKL (Immudex cat # JD2163-PfBC ...
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No products found
because this supplier's products are not listed.
Taylor K Loe, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Coverslips were dehydrated in an ethanol series (70%, 90% and 100%) and hybridized at RT with a TRITC-OO[TTAGGG]3 labeled PNA probe (PNA Bio) in hybridization buffer (70% formamide ...
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No products found
because this supplier's products are not listed.
Maria V. Sinegubova, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The PCR product was restricted using AbsI (Sibenzyme, Novosibirsk, Russia) and NheI (Fermentas ...
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No products found
because this supplier's products are not listed.
Catherine A. Cotter, et al.,
bioRxiv - Microbiology 2022
Quote:
... wells were washed three times with 250 μl PBS + 0.05% Tween-20 (PBS-T, Accurate Chemical). Plates were blocked for 2 h at room temperature with 200 μl PBS-T + 5% nonfat milk and subsequently washed three times with PBS-T prior to incubation with a series of eight 4-fold dilutions of mouse sera for 1 h at room temperature ...
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No products found
because this supplier's products are not listed.
Aaron Lanz, Grant Gordon, Jaideep Bains,
bioRxiv - Neuroscience 2020
Quote:
... Virus was pressure injected into the brain three times with a Nanoject II apparatus (Drummond Scientific Company), each injection at a volume of 69 nL and placed increasingly deeper into the brain (AP ...
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No products found
because this supplier's products are not listed.
Tatiana Agback, et al.,
bioRxiv - Molecular Biology 2021
Quote:
The PCR product was cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc) between Bsa I and Xho I restriction sites ...
-
No products found
because this supplier's products are not listed.
Krithika Rao, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were washed in PBS (3 times for 5 mins) prior to nuclear counterstaining with DAPI in mounting media (Vector BioLabs).
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No products found
because this supplier's products are not listed.
Chongping Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... the membrane was washed with 1 × TBST for three times and then incubated in a 1:5000 dilution of goat anti-mouse IgG secondary antibody (Signalway Antibody LLC, Maryland, USA) for 1 hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Ricardo J. Ferreira, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli gyrase supercoiling assay kit from Inspiralis (Norwich Research Park ...
-
No products found
because this supplier's products are not listed.
Chengyan Wu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The CCK8 kit was purchased from Abbkine. The reactive oxygen detection kit for tissue sections was purchased from Shanghai Pebble Biotechnology Company ...
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No products found
because this supplier's products are not listed.
Katia K Mattis, et al.,
bioRxiv - Physiology 2022
Quote:
... Single larvae per well of a 96-well PCR plate were homogenised with a 1.4 mm acid washed zirconium bead (OPS diagnostics, USA) in 88 µL ice cold PBS using a MiniG™ 1600 homogenizer (SPEX® SamplePrep ...
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Adenovirus
Cat# KC30996,
AdenoMag 200µL + Magnetic Plate MF96000, USD $654.00/KIT
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Adam T. Vogel, Shelley J. Russek,
bioRxiv - Neuroscience 2021
Quote:
... using the NeuroMag Magnetofection™ kit (Oz Biosciences #KC30800). Plasmid DNA was diluted in NBM and added to the NeuroMag Transfection Reagent ...
-
No products found
because this supplier's products are not listed.
Shoupeng Wei, et al.,
bioRxiv - Neuroscience 2023
Quote:
Hito Golgi-Cox Kit (Hitobiotec Corp., Wilmington, DE, USA) was used to reveal the structure and density of dendritic spines in the mPFC37–39 ...
-
No products found
because this supplier's products are not listed.
Sujoy Chatterjee, et al.,
bioRxiv - Immunology 2020
Quote:
... Gel shift assays were done with EMSA kits (Signosis Inc). 5 μg nuclear extracts were incubated with 1× binding buffer and biotin-labeled probe for 30 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Zhouyi Rong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mouse Interferon Gamma (IFNg) ELISA Kit (RD-IFNg-Mu, Reddot biotech), Mouse Interleukin 6 (IL6 ...
-
No products found
because this supplier's products are not listed.
Syed Moiz Ahmed, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Endogenous TOP1cc were detected by Human Topoisomerase ICE kit (Topogen, TG1020-0) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yuka Sakata, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Commercially-available clinical-grade enzyme-linked immunosorbent assay (ELISA) kits (DRG International) were used to detect pregnenolone (EIA-4170) ...
-
No products found
because this supplier's products are not listed.
Anh T. P. Ngo, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Thrombin generation was calculated against a thrombin calibration curve (calibration kit, Diapharma).
-
No products found
because this supplier's products are not listed.
Thomas Klein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... iPSC were generated using the StemRNA 3rd Gen reprogramming kit (Reprocell, Maryland, USA) for all male cell lines (FD-1 ...
-
No products found
because this supplier's products are not listed.
Yonghui Ding, et al.,
bioRxiv - Bioengineering 2023
Quote:
... were expanded in human SMC growth medium kit (Cell Applications, Inc., 311K-500) under the standard culture condition (37 °C with 5% CO2 in a humid environment ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The IgG ELISAs for RBD were conducted by using Antibody IgG Titer Serologic Assay Kit (AcroBiosystems, RAS-T059). According to the manufacturer’s protocol ...