-
No products found
because this supplier's products are not listed.
Francisco J. Calero-Cuenca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Reverse transcription quantitative PCR (RT-qPCR) was performed using Power SYBR Green PCR MasterMix (Alfagene) according to the manufacturer’s instructions and using primers forward and reverse at 0,25 μM (final concentration ...
-
No products found
because this supplier's products are not listed.
Enrique Lin-Shiao, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and 10 μg U6-ELS77 plasmids using polyethylenimine (Polyscience Inc.). Transfected cells were switched into Optimem (Gibco ...
-
No products found
because this supplier's products are not listed.
Fenglei Li, et al.,
bioRxiv - Immunology 2023
Quote:
... 106 T cells suspended in HBS/HSA were introduced into heated FCS2 flow chambers (Bioptechs) and allowed to interact with T22-containing SLBs as for intracellular Ca2+-imaging ...
-
No products found
because this supplier's products are not listed.
Maxime Mivelaz, et al.,
bioRxiv - Biophysics 2019
Quote:
... instrument calibration was performed by imaging 100-nm biotinylated Gold nanoparticles (Cytodiagnostics) with 532 nm excitation and 100 ms time-resolution over 10 s ...
-
No products found
because this supplier's products are not listed.
Dmitry Senko, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 32 lipid classes (PC, PCO, PE, PEO, PEP, PS, PA, PG, BMP, PI ...
-
No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
... MicroRT tubing (Mitegen, RT-T1) with 5 μl of reservoir in the end was placed over the crystals to prevent dehydration ...
-
No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
-
No products found
because this supplier's products are not listed.
Yasmin Fareed, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
Pooled biofluid samples for matrix-matched calibration were obtained as follows: lithium-heparin plasma was purchased from Innovative Research (Novi, Michigan). Pooled urine was provided by an Austrian female volunteer who avoided the consumption of food and use of cosmetics stored in plastic containers ...
-
No products found
because this supplier's products are not listed.
Florian Geiger, et al.,
bioRxiv - Biophysics 2020
Quote:
... and AlexaFluor 647 detection (49009, Chroma Technology). Representative data are presented in Supplementary Figure 5.
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No products found
because this supplier's products are not listed.
Ricardo M. Santos, Anton Sirota,
bioRxiv - Neuroscience 2020
Quote:
All in vitro tests were done in a stirred calibration buffer kept at 37 °C using a circulating water pump (Gaymar heating/cooling pump, Braintree Scientific, Inc., USA) connected to the calibration beaker ...
-
No products found
because this supplier's products are not listed.
Wei-Wen Lim, et al.,
bioRxiv - Pathology 2019
Quote:
The amount of total tissue collagen was quantified using colorimetric detection of hydroxyproline using the Quickzyme Total Collagen assay kit (Quickzyme Biosciences) performed according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Suheda Erener, et al.,
bioRxiv - Physiology 2019
Quote:
... C57BL/6 wild-type littermates and miR-216a KO mice were fed a 60% HFD diet (D12492i, Research Diets, Cedarlane, Burlington, Canada) starting at 6-8 weeks of age for 8 weeks ...
-
No products found
because this supplier's products are not listed.
Qiang Sun,
bioRxiv - Plant Biology 2021
Quote:
... Xylem segments were then silver-enhanced at RT for 15 min with a silver enhancement kit (BBI Solutions, UK, Batch No. 12348). After wash with DD H2O and dehydration via an ethanol series ...
-
No products found
because this supplier's products are not listed.
Brian W. Ji, et al.,
bioRxiv - Microbiology 2019
Quote:
... inverted sterile 200 µL pipette tips (Rainin RT-L200F) were used to core out a small sample from the stool ...
-
No products found
because this supplier's products are not listed.
Rima Gnaim, et al.,
bioRxiv - Microbiology 2020
Quote:
... 110 isolated bacteria colonies were transferred to 2 mL liquid marine broth (3.7 g·L-1) and kept for overnight at 32°C in a shaker incubator (180 RPM, Incu-Shaker Mini, Benchmark Scientific). The bacteria were stored in glycerol (final concentration of 25% ...
-
No products found
because this supplier's products are not listed.
Pradeep Ramalingam, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
-
No products found
because this supplier's products are not listed.
Xiaoxin X. Wang, et al.,
bioRxiv - Pathology 2020
Quote:
... UnoVue HRP secondary antibody detection reagent (Diagnostic BioSystems, Pleasanton, CA) was applied followed by DAB chromogen ...
-
No products found
because this supplier's products are not listed.
Magda Luciana Atilano, et al.,
bioRxiv - Neuroscience 2022
Quote:
... They were then subjected to an injection into the thorax with 32 nl heat-killed Staphylococcus aureus NCTC8325-4 (BAC) or PBS using a microinjector (Nanoject II; from Drummond Scientific).
-
No products found
because this supplier's products are not listed.
Román Ramos Báez, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the products were purified using the EZNA Cycle Pure® Kit or the NEB Monarch® PCR & DNA Cleanup Kit and confirmed by sequencing (MCLab or Genewiz).
-
No products found
because this supplier's products are not listed.
Maximilian Bunz, et al.,
bioRxiv - Microbiology 2023
Quote:
... or SiR-DNA dye (1 µM, 90 min, RT; Tebu-Bio). Three final washing steps were performed ...
-
No products found
because this supplier's products are not listed.
Kristina Bartmann, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... washed three times with PBS (PAN Biotech), and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Anne-Perrine Foray, et al.,
bioRxiv - Immunology 2021
Quote:
... and an anti-IFNγ capture and detection antibody pair (U-CyTech Biosciences) were used ...
-
No products found
because this supplier's products are not listed.
Leanne E. Wybenga-Groot, C. Jane McGlade,
bioRxiv - Biochemistry 2020
Quote:
... 10% (w/v) PEG8000 at rt using a Mosquito robot (TTP Labtech). Small rod-like crystals were observed after approximately five months ...
-
No products found
because this supplier's products are not listed.
C Bourges, et al.,
bioRxiv - Genetics 2020
Quote:
Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase, 30 cycles, Micellula DNA Emulsion & Purification Kit (Chimerx)) using primers containing SfiI restriction sites ...
-
No products found
because this supplier's products are not listed.
Sanne van der Niet, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Dihydrochloride) detection was performed using anti dinitrophenol (DNP) (Polyclonal Anti-DNP, Oxford Biomedical Research). All antibodies were diluted in 1% BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Zhexin Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 3 times at 10,000 rpm for 5 s (IKA TC10 basic ULTRA-TURRAX® homogenizer with S10N-5G dispersing element ...
-
No products found
because this supplier's products are not listed.
Sarah Bahraoui, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Quantitative PCR was performed on 6,25ng of cDNA using the SensiFAST™ SYBR® No-ROX kit (Bioline, Meridian Life Science© Company) and a LightCycler® 480 Detection system (Roche) ...
-
No products found
because this supplier's products are not listed.
Seesandra V. Rajagopala, et al.,
bioRxiv - Genomics 2022
Quote:
... and 7.25 μl PCR Certified Water (Teknova). RNA was reverse transcribed at 50ºC for 15 minutes ...
-
No products found
because this supplier's products are not listed.
Dena Ennis, et al.,
bioRxiv - Microbiology 2023
Quote:
... Samples were separated by HPLC with fluorescence detection on a TSKgel Amide-80 column (Tosoh Bioscience, Tokyo, Japan) with a linear gradient of a 50 mM ammonium formate/acetonitrile solvent system ...
-
No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated for 1.5 hours at RT with the following primary antibodies: LBR (1:200, Atlas Antibodies HPA062236), LaminB1 (1:500 ...
-
No products found
because this supplier's products are not listed.
Marie-Laure Fogeron, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
-
No products found
because this supplier's products are not listed.
Marianne E Emmert, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... through detection of the 7-amino-4-methylcoumarin (AMC) labeled fluorogenic peptide substrates Z-LLE-AMC (Boston Biochem #S-230) and LLVY-AMC (Chemicon #APT280) ...
-
No products found
because this supplier's products are not listed.
Jingyao Li, et al.,
bioRxiv - Genomics 2021
Quote:
... 10 nl of 1 uM barcoded DRUG-seq RT primers were then dispensed into each well using an Echo 555 Liquid Handler (Labcyte). Plates were sealed (BioRad ...
-
No products found
because this supplier's products are not listed.
Ahmed Ali, et al.,
bioRxiv - Biophysics 2023
Quote:
Mouse sera were diluted ten times in PEG dilution buffer (PEGD50-1, Life Diagnostics, Inc.), and sequentially in 1:3 dilution steps added to the wells of PEG-BSA coated ELISA plates (PBSA20PL ...
-
No products found
because this supplier's products are not listed.
Clara Sidor, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... was amplified by PCR from the mNeonGreen vector (Allele Biotechnology), with the exclusion of the stop codon and the addition of a C-terminal linker.
-
No products found
because this supplier's products are not listed.
Jingjing Lou, et al.,
bioRxiv - Microbiology 2023
Quote:
... mAID CDS PCR amplified (TruFi™ DNA Polymerase; Azura Genomics) from plasmid plinker-AID-Ty-HXGPRT-LoxP using primers P8 and P9 ...
-
No products found
because this supplier's products are not listed.
Suchitra Joshi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The estradiol levels (n=4) were also measured using an ELISA assay (#ES180S-100 Calbiotech, detection range 3 to 300 pg/mL). The intra-assay variation was 5.9% in these assays.
-
No products found
because this supplier's products are not listed.
Cody Juguilon, et al.,
bioRxiv - Physiology 2021
Quote:
... The beads with EC were washed several times and cultured in mouse EC cultured medium (Cell Biologics). When the cells were confluent ...
-
No products found
because this supplier's products are not listed.
M. Koltun, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Each time tissues were homogenized using Bullet Blender with 0.5 mm RNase Free Zirconium Oxide Beads (Next Advance) in RLT buffer from RNeasy Plus Micro Kit (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Eva Jarc Jovičić, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The retention time and at least six diagnostic ions for each lipid mediator were confirmed by means of external standards (Cayman Chemical/Biomol GmbH). Quantification was achieved by calibration curves for each lipid mediator ...
-
No products found
because this supplier's products are not listed.
Elisabet Perez-Coronel, J. Michael Beman,
bioRxiv - Genomics 2021
Quote:
... Temperature and dissolved oxygen were measured at the time of sample collection using a ProODO YSI probe (YSI Inc., Yellow Springs, OH, USA).
-
No products found
because this supplier's products are not listed.
Mindy Engevik, et al.,
bioRxiv - Cell Biology 2022
Quote:
Albumin concentration in stool was determined using a commercial kit (Albumin ELISA Kit, Genway, GWB-282C17) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Eric T. Son, et al.,
bioRxiv - Immunology 2021
Quote:
... QuickSwitch Custom Tetramer Kits (MBL International) were utilised to generate multiple tetramers with selected peptides in order to screen an array of pMHC epitopes ...
-
No products found
because this supplier's products are not listed.
Timur O Yarovinsky, et al.,
bioRxiv - Immunology 2019
Quote:
... We used HBsAg ELISA kit from XpressBio and the HBsAg standard from CellBioLabs to measure serum HBsAg in the chronic HBV infection model.
-
No products found
because this supplier's products are not listed.
Wyatt G. Paltzer, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Aniline Blue Stain Kit” (Newcomer Supply, #9179B) following the kit protocol ...
-
No products found
because this supplier's products are not listed.
Stavros Giaglis, et al.,
bioRxiv - Immunology 2021
Quote:
... utilizing the human TAT Complexes ELISA Kit (Assaypro) and the Imuclone D-Dimer ELISA Kit (American Diagnostica ...
-
No products found
because this supplier's products are not listed.
Luca Valzania, Aya Alami, Pierre Léopold,
bioRxiv - Developmental Biology 2023
Quote:
... using the 20-Hydroxyecdysone ELISA kit (Bertin bioreagent, #A05120) as recommended by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Ichiro Yamauchi, et al.,
bioRxiv - Physiology 2023
Quote:
... TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA). We diluted serum samples 25-fold with phosphate buffered saline when we measured human TSH levels ...
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
-
No products found
because this supplier's products are not listed.
Caroline Passaes, et al.,
bioRxiv - Immunology 2019
Quote:
... Culture supernatants were assayed on day 7 using an SIV p27 Antigen ELISA Kit (Zeptometrix). Antiviral activity was calculated as log10 (mean p27 ng/mL in SIV-infected CD4+ T-cell cultures without CD8+ T-cells ...