-
No products found
because this supplier's products are not listed.
Shalini Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The N- and C-peptides were labeled with maleimide-derivatized DY549P1 (Dyomics), DY649P1 (Dyomics) ...
-
No products found
because this supplier's products are not listed.
Christian S. Stevens, et al.,
bioRxiv - Microbiology 2022
Quote:
... and fluorescent probe (5’-6FAM-ACAGACGTTGTATA+C+CAT+G-TMR) (TIB MOLBIOL) RT-qPCR was performed using the following cycle ...
-
No products found
because this supplier's products are not listed.
Ken Shirato, Jun Takanari, Takako Kizaki,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... the cells were co-treated with the indicated concentrations of ETAS®50 or dextrin and 100 ng/mL of SARS-CoV-2 spike recombinant protein S1 subunit (Arigo Biolaboratories, Hsinchu City, Taiwan) for 1 to 24 h ...
-
No products found
because this supplier's products are not listed.
Matthew L. Fabian, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Aliquots of 1 μL of TRV1 and recombinant TRV2 were electroporated into 50 μL tubes of Agrobacterium tumifasciens strain GV3101 cells (Intact Genomics, St. Louis MO, USA) using a Bio-Rad Gene Pulser II and Pulse Controller electroporation system set to 2.2 kV ...
-
No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Jeremy Rich, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and the peroxidase/diaminobenzidine Rabbit PowerVision kit (ImmunoVision Technologies), respectively ...
-
No products found
because this supplier's products are not listed.
Susanne Hellmuth, Olaf Stemmann,
bioRxiv - Cell Biology 2024
Quote:
... rabbit anti- histone H2A (1:1,000; 11-7017, Abeomics), rabbit anti-pS10-histone H3 (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Delphine Planas, et al.,
bioRxiv - Immunology 2021
Quote:
... 2) a CE-Marked ELISA assay for detection of IgG against the full-length recombinant N protein from Epitope Diagnostics (EDITM Novel coronavirus COVID-19 IgG). The test has a specificity of 96% and a sensitivity of 81% after 28 days POS in our hands ...
-
No products found
because this supplier's products are not listed.
Helen J. von Richthofen, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were treated 2h at 37°C with neutrophil elastase (Elastin Products Company), cathepsin G (Biocentrum) ...
-
No products found
because this supplier's products are not listed.
Valentina Casa, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Immunization of the rabbits was performed by Absea (Beijing, China). Antibodies were purified from the serum using the proteins used for immunization coupled to sulfolink (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Biswajoy Ghosh, et al.,
bioRxiv - Biophysics 2023
Quote:
... Rabbit anti-mouse polyclonal anti-Collagen-IV from MD Biosciences. Goat anti-rabbit with AlexaFluor 488 and 647 from Invitrogen ...
-
No products found
because this supplier's products are not listed.
Erika Riederer, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1 µM valinomycin at 30 °C using 100 nM of 14C Asp (Moravek Biochemicals) as previously described.(33 ...
-
No products found
because this supplier's products are not listed.
Emilie Pondeville, et al.,
bioRxiv - Genetics 2019
Quote:
... Hemocytes were incubated at 4°C overnight with a rat anti-mCD8 antibody (Ancell) diluted 1:100 or a rabbit anti-PPO2 (Fraiture ...
-
No products found
because this supplier's products are not listed.
Elsa Mazari-Arrighi, et al.,
bioRxiv - Bioengineering 2021
Quote:
... rabbit anti-rat albumin antibody (RaRa/ALB/7S, Nordic-MUbio, Netherlands) was used as primary antibody and biotinylated goat anti-rabbit antibody (VECTASTAIN Elite ABC HRP Kit ...
-
No products found
because this supplier's products are not listed.
Eva Gracia Villacampa, et al.,
bioRxiv - Genetics 2020
Quote:
... the slides were dried at 40°C in a hybridization oven (HB-1000 Hybridizer, LabRepCo) for 1 hour and 45 min ...
-
No products found
because this supplier's products are not listed.
Lindsay Smith, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... zebrafish were incubated for 5 min at 28.5°C with optovin 6b8 (ID 5705191l; ChemBridge), an optovin analog (Kokel et al. ...
-
No products found
because this supplier's products are not listed.
Y Audrey Hay,
bioRxiv - Neuroscience 2021
Quote:
... France) mice temperature was monitored and maintained at 34-36 °C using a heating blanket (Basi). A small burr hole was drilled at LDTg coordinates AP = -4.92 mm and ML = 1 mm and an optical fiber (300 μm ...
-
No products found
because this supplier's products are not listed.
Adrian S. Monthony, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The callus induction medium (hereafter referred to as LT-C) consisted of MS (M524; Phytotechnology Laboratories) nutrients ...
-
No products found
because this supplier's products are not listed.
Kelsey M. Tyssowski, Jesse M. Gray,
bioRxiv - Neuroscience 2019
Quote:
... The temperature was maintained by putting the plate on a 37°C warming plate (Bel-Art). The CO2 was maintained at 5% throughout the duration of the recording using the base provided with the Axion Lumos system ...
-
No products found
because this supplier's products are not listed.
Tamás Bakos, et al.,
bioRxiv - Immunology 2024
Quote:
... Soluble terminal C complex (sTCC, sC5b9) was measured with an ELISA kit from Svar Life Science AB (Malmö ...
-
No products found
because this supplier's products are not listed.
István Fodor, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2) incubation with a rabbit anti-ap-GnRH/CRZ antiserum (#AS203-2, EZbiolab; antigen was a synthetic undecapeptide ...
-
No products found
because this supplier's products are not listed.
Rubika Balendra, et al.,
bioRxiv - Neuroscience 2022
Quote:
Poly(PR) 20-mer dipeptide repeat protein with a C-terminal FLAG tag was purchased from CSBio and verified by mass spectrometry ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Charlène Iltis, et al.,
bioRxiv - Immunology 2021
Quote:
... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
-
No products found
because this supplier's products are not listed.
Tobias Butelmann, et al.,
bioRxiv - Bioengineering 2021
Quote:
... an LM was taken out from the medium (30 °C) and weighed on a precision scale (Adam Equipment, UK). To determine the dry weight of the cells ...
-
No products found
because this supplier's products are not listed.
Timothy O'Leary, et al.,
bioRxiv - Biochemistry 2021
Quote:
Cells were washed with DPBS and incubated with cholPEGNTA (Nanocs # PG2-CSNT-2k; 10 μM, 1 hr, 37°C) After washing twice with DPBS ...
-
No products found
because this supplier's products are not listed.
Peter A. van der Ley, et al.,
bioRxiv - Immunology 2021
Quote:
The prefusion stabilized HexaPro spike protein27 with and without a 3xGGGS spacer and mCRAMP sequence added to the C-terminus were produced by Celonic AG (Basel ...
-
No products found
because this supplier's products are not listed.
Rebekah P. Dyer, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then lysed at 23 °C with shaking at 500 rpm for 30 min in DWP Lysis Buffer: 100 μL per well of SoluLyse (Genlantis) plus benzonase nuclease (75 U/mL ...
-
No products found
because this supplier's products are not listed.
Cornelia Blume, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
-
No products found
because this supplier's products are not listed.
Yongchan Lee, et al.,
bioRxiv - Biochemistry 2019
Quote:
... UniProt ID Q01650) and CD98hc isoform c (SLC3A2 isoform 1; UniProt ID P08195-1) were amplified from human universal reference cDNA (ZYAGEN) and cloned individually into the pEG BacMam vector (84) ...
-
No products found
because this supplier's products are not listed.
Jugal Mohapatra, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and heated to 90 °C for 2 min while bubbling with nitrogen gas in N,N-dimethylformamide (DMF, Oakwood Chemical). Fmoc deprotection was carried out with 20% piperidine (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Margot Meyers, et al.,
bioRxiv - Biochemistry 2023
Quote:
... was treated with either DMSO vehicle or covalent ligand at 37 °C for 30 min and subsequently treated with 0.1 μM IA-rhodamine (Setareh Biotech) for 1 h at RT in the dark ...
-
No products found
because this supplier's products are not listed.
Isabelle Petit-Härtlein, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.1 µL of protein solution and 0.1 µL of reservoir were mixed to equilibrate against 45 µL reservoir solution at 20°C in 96-well CrystalDirect plates (MiTeGen) and automatically imaged in RockImager robot (Formulatrix) ...
-
No products found
because this supplier's products are not listed.
Samuel Schmidt, et al.,
bioRxiv - Cell Biology 2020
Quote:
... incubated 24h at 4 °C) were used as substrates and prepared in Willco dishes (35 mm, Willco Wells B.V., HBSt-3522) as described46 ...
-
No products found
because this supplier's products are not listed.
Tomasz Zieliński, et al.,
bioRxiv - Biophysics 2022
Quote:
... and kept in the CO2 (37°C and 5%CO2/95% air atmosphere) for 24 hours in the DMEM (ATCC, LGC Standards) supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Genevieve. McCluskey, et al.,
bioRxiv - Immunology 2021
Quote:
The effect of FH on protein C (PC) activation was monitored by combining FH (0.5-125nM) with 1.75nM thrombin and 200nM PC (Haematologic Technologies, Inc., USA) in the absence or presence of 10nM soluble recombinant TM (R&D Systems/Biotechne ...
-
No products found
because this supplier's products are not listed.
Bruno Motta Nascimento, Nikhil Unni Nair,
bioRxiv - Bioengineering 2020
Quote:
... coli were hydrolyzed 6 M HCl at 100 °C for 4 h in a vacuum sealed tube (Chemglass, #CG-4025-01). Acid was immediately removed with a vacuum centrifuge and pellet was resuspended in deionized water ...
-
No products found
because this supplier's products are not listed.
Quentin Sabbagh, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 4°C) by size exclusion chromatography (SEC) using 70 nm Original qEV columns associated with an automatic fraction collector (Izon Science), according to the manufacturer’s protocol and recommendations from the International Society for Extracellular Vesicles (ISEV ...
-
No products found
because this supplier's products are not listed.
Henriette Frikke-Schmidt, et al.,
bioRxiv - Physiology 2020
Quote:
... The plates for the islets were placed on a mat heated at 37 °C on a H101A ProScan motorized stage (Prior Scientific Instruments Ltd ...
-
No products found
because this supplier's products are not listed.
Alan P. R. Lorenzetti, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cell pellets were resuspended in Milli-Q water and disrupted at 4°C using ceramic beads (Mo Bio Laboratories) and a Precellys 24 homogenizer (Bertin Corp). Protein content was determined by bicinchoninic acid assay (BCA ...
-
No products found
because this supplier's products are not listed.
W. Coles Keeter, et al.,
bioRxiv - Immunology 2023
Quote:
... 8-12-week-old male mice were placed on a high fat diet with 0.15% added cholesterol (HFD-C, fat: 60% kcal; carbohydrate: 26% kcal, Bio-Serv F3282) for 28 weeks until sacrifice.
-
No products found
because this supplier's products are not listed.
Cameron Durfee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... They were then baked at 65°C for 20 minutes to deparaffinize and then rehydrated with three consecutive washes in CitriSolv (Decon Labs) for 5 min each followed by graded alcohols as above ...
-
No products found
Tao Cheng, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 15 minutes at 4°C) from blood isolated by sub-mandibular bleeding and used for the BUN assay (BUN-Urea, BioAssay Systems) following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Grigorios Fanourgakis, et al.,
bioRxiv - Genetics 2024
Quote:
... After washing cells were incubated with 20 μg/ml with Hoechst 33342 (Thermo Fischer Scientific H3570) for 1 hour at 32°C with constant shaking and protected from light and then with 30nM DRAQ7 (Biostatus DR71000) for 5 minutes on bench ...
-
No products found
because this supplier's products are not listed.
Philippe Dehio, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The slides were first plasma cleaned and PEG-coated at 4 °C overnight with 200µg/ml poly(L-lysine)-PEG (SuSoS, PLL(20)-g[3.5]-PEG(2) ...
-
No products found
because this supplier's products are not listed.
Claudia Ctortecka, et al.,
bioRxiv - Biochemistry 2024
Quote:
... the proteoCHIP EVO 96 is inserted into the cellenONE® using a dedicated holder (C-PEVO-96-CHB, Scienion, Berlin, Germany), 150nL of master mix (0.2% DDM (D4641-500MG ...
-
No products found
because this supplier's products are not listed.
Stijn Mouton, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... the overnight cultures were used both to create glycerol stocks for long-term storage at -80°C and for plasmid DNA extractions using the Plasmid DNA Extraction Mini Kit (Favorgen®). The extracted plasmids were used for Sanger sequencing using a universal T444T forward sequencing primer (Table S1).
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
... or conjugated in-house with X8 antibody-labeling kits (Standard BioTools) and stored at 4°C in Antibody Stabilizer (Boca Scientific) supplemented with 0.05% sodium azide ...
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Aaron R. Tipton, Gary J. Gorbsky,
bioRxiv - Cell Biology 2021
Quote:
... Rabbit anti-Nuf2 (P. Todd Stukenberg; University of Virginia) and human anti-Centromere Antibodies (ACA; Antibodies Incorporated). Secondary antibodies used were goat anti-rabbit antibodies conjugated fluorescein isothiocyanate (FITC ...