-
No products found
because this supplier's products are not listed.
Duyen Huynh, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
-
No products found
because this supplier's products are not listed.
Marco Brustolin, et al.,
bioRxiv - Microbiology 2021
Quote:
... Then cells were labeled with the monoclonal anti-CHIKV E2 envelope glycoprotein clone CHK-48 made in mouse (BEI Resources, Manassas, VA, USA) and the polyclonal anti-Zika virus envelope protein made in rabbit (GeneTex, Irvine, CA, USA). The antibodies used for immunofluorescence included Alexa Fluor 488 conjugated goat anti-rabbit IgG (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Agnieszka Dabrowska, et al.,
bioRxiv - Microbiology 2020
Quote:
Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
-
No products found
because this supplier's products are not listed.
Margarita V. Rangel, et al.,
bioRxiv - Microbiology 2021
Quote:
... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
-
No products found
because this supplier's products are not listed.
Jiahui Ding, et al.,
bioRxiv - Immunology 2021
Quote:
... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
-
No products found
because this supplier's products are not listed.
Derek D.C. Ireland, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies used include: human polyclonal anti-Zika virus antibody (Kerafast EVU302, MA) or mouse anti-ZikV E protein mAb (Fitzgerald ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
-
No products found
because this supplier's products are not listed.
Sara B. York, et al.,
bioRxiv - Microbiology 2021
Quote:
... Zika Envelope (EastCoast Bio; HM325), Zika capsid (GeneTex ...
-
No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
No products found
because this supplier's products are not listed.
Tsukasa Oikawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Retroviruses with the vesicular stomatitis virus–G (VSV-G) envelope were produced by transfection of GP2-293 cells (Clontech) with the pRetroX construct and pVSV-G (Clontech ...
-
No products found
because this supplier's products are not listed.
Nipun Verma, et al.,
bioRxiv - Genetics 2024
Quote:
... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
-
No products found
because this supplier's products are not listed.
Donald C. Koroma, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and envelope plasmid pVSV-G (G178153, GenScript) at a 10:10:1 ratio using Lipofectamine™ 2000 according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Danny Simpson, et al.,
bioRxiv - Genomics 2023
Quote:
... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
-
No products found
because this supplier's products are not listed.
Carolyn Marquis, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The time between nuclear envelope breakdown (NEB) and anaphase onset (AO ...
-
No products found
because this supplier's products are not listed.
Zunlong Ke, et al.,
bioRxiv - Microbiology 2023
Quote:
... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Dhiviya Vedagiri, et al.,
bioRxiv - Immunology 2020
Quote:
... except anti-flaviviral envelope 4G2 (Merck Millipore), β-Tubulin ...
-
No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
Cellular immunofluorescence for nuclear envelope proteins was performed using 1205Lu cells plated on cleaned coverslips (#1.5, 22 mm x 22 mm, Corning) for 24-72 hours depending on experimental conditions ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
-
No products found
because this supplier's products are not listed.
Chen-xi Zhang, et al.,
bioRxiv - Immunology 2020
Quote:
The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
-
No products found
because this supplier's products are not listed.
Eleonora Sementino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
-
No products found
because this supplier's products are not listed.
Ronnie Blazev, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Virus was purified by heparin affinity chromatography on an Akta FPLC (HiTrap, GE Healthcare), ultra-centrifuged overnight and resuspended in sterile Ringer’s solution ...
-
No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
-
No products found
because this supplier's products are not listed.
Ding-Chin Lee, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... rabbit anti-SARS-CoV-2 envelope (Cell signaling, #74698), rabbit anti-GAPDH (Genetex ...
-
No products found
because this supplier's products are not listed.
Jonathan Burnie, et al.,
bioRxiv - Microbiology 2021
Quote:
The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
-
No products found
because this supplier's products are not listed.
Jiang-Hui Wang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Olesya N Gusachenko, et al.,
bioRxiv - Microbiology 2021
Quote:
Virus injections of pupae were performed with insulin syringes (BD Micro Fine Plus ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Zhihua Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
-
No products found
because this supplier's products are not listed.
Jamie C. Moffa, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus titers were measured using p24 enzyme-linked immunosorbent assays (ELISA) (R&D Systems, MN, USA). CD4+ Jurkat T cells were incubated with NL4-3 or NL4-3dNef viruses at MOI ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... the SARS-CoV-2 envelope (E) protein (ABclonal RP01263) or the SARS-CoV-2 spike (S ...
-
No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). SARS-CoV-2 NSP1 (97-095) ...
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.
-
No products found
because this supplier's products are not listed.
Aaron R. Massey, et al.,
bioRxiv - Neuroscience 2020
Quote:
... rabbit polyclonal antibody to WNV envelope protein (1:800, Novus Biologicals #NB100-56744), and rabbit polyclonal antibody to Iba-1 (1:200 ...