-
No products found
because this supplier's products are not listed.
Anne-Sophie Banneville, et al.,
bioRxiv - Microbiology 2021
Quote:
200 ng of relaxed DNA (relaxed pHOT-1 DNA, 2.4 kb) (Topogen) was incubated for 15 min at 4°C in the absence or presence of increasing concentrations of DdrC in 25 μl of buffer composed of 40 mM Tris-HCl pH7.8 ...
-
No products found
because this supplier's products are not listed.
Isadora Matias, et al.,
bioRxiv - Neuroscience 2021
Quote:
Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore ...
-
No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... with protein ladder (Gel Company FPL-008). Gels were imaged with a Sapphire molecular imager (Azure Biosystems ...
-
No products found
because this supplier's products are not listed.
Abhishek Dutta, Fabien Dutreux, Joseph Schacherer,
bioRxiv - Genomics 2022
Quote:
Genomic DNA was extracted from the MA lines using the Omega yeast DNA kit (Life Science Products). DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England Biolabs ...
-
No products found
because this supplier's products are not listed.
Verica Vasić, et al.,
bioRxiv - Neuroscience 2022
Quote:
... As primary antibody a polyclonal rabbit anti-human EGFL7 antibody (1:50, ReliaTech GmbH) was applied ...
-
No products found
because this supplier's products are not listed.
Víctor M. Hernández-Rocamora, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The protein was labelled with Dy647-maleimide probe (Dyomics, Germany) following instructions from the manufacturer ...
-
No products found
because this supplier's products are not listed.
Ezekiel C. Thomas, Amber Ismael, Jeffrey K. Moore,
bioRxiv - Cell Biology 2020
Quote:
... The mNeonGreen plasmid DNA was provided by Allele Biotechnology and Pharmaceuticals (San Diego ...
-
No products found
because this supplier's products are not listed.
Dario De Felice, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... DNA gels images were acquired with an UV scanner (UVITEC). Real-time quantitative PCR was performed with qPCRBIO SyGreen Mix (PCRBiosystems ...
-
No products found
because this supplier's products are not listed.
Alessandro Gori, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The detector antibody (biotinylated CD9, CD63, CD81 antibodies by Ancell or anti-band 3 from Santa Cruz) solutions (0.3 µg/ml ...
-
No products found
because this supplier's products are not listed.
Abdolhossein Zare, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and motoneurons were enriched by p75NTR antibody (clone MLR2, Biosensis) panning ...
-
No products found
because this supplier's products are not listed.
Domagoj Cikes, et al.,
bioRxiv - Genetics 2023
Quote:
... Fifty ug of protein was assayed with 0.2 µCi of [14C]-phosphoethanolamine (P-Etn) (American Radiolabeled Chemical) in 50µl of reaction mixture of 50 mM MgCl2 ...
-
No products found
because this supplier's products are not listed.
Chiara E. Geyer, et al.,
bioRxiv - Immunology 2022
Quote:
... IL-6 concentration was determined using antibody pairs from U-CyTech Biosciences (Human IL-6 ELISA ...
-
No products found
because this supplier's products are not listed.
Esther B. Florsheim, et al.,
bioRxiv - Immunology 2023
Quote:
... or rabbit polyclonal anti-Fluoro-Gold primary antibody (1:1000 Fluorochrome) in the same blocking solution overnight for 16 h and then with Alexa Fluor 594-conjugated donkey anti-rabbit IgG secondary fluorescent antibody (1:500 dilution ...
-
No products found
because this supplier's products are not listed.
Bassem D. Khalil, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The resulting NR2F1 LBD was used to screen for potential agonists from a library of 110,000 drug-like small molecules (ChemBridge, USA) using computational docking ...
-
No products found
because this supplier's products are not listed.
Martin H. Berryer, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Starting on day 2 human induced neural progenitor-like cells were harvested with Accutase (Innovative Cell Technology, Inc., AT104-500) and re-plated at 15,000 cells.cm−2 in Astrocyte Medium (ScienCell ...
-
No products found
because this supplier's products are not listed.
Anthony Hung, et al.,
bioRxiv - Genomics 2021
Quote:
iPSC-chondrocytes were treated with a cyclic tensile strain regimen that is known to induce an OA-like phenotype using the Flexercell FX6000 Tension System (Flexcell International)23–26 ...
-
No products found
because this supplier's products are not listed.
MJ Grubisha, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and the repair oligo (200ng/μL) were mixed for electroporation using a Biorad Gene Pulser XCell and custom electroporation chamber (Protech International Inc., cat# LF501-P1X10). sgRNAs and protein were diluted with Opti-MEM (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Amy R. Rappaport, et al.,
bioRxiv - Immunology 2021
Quote:
... Plates were incubated with anti-nucleocapsid protein primary antibody cocktail (clones HM1056 and HM1057) (EastCoast Bio, North Berwick, ME) for 60 minutes at 37°C (Battelle Memorial Institute ...
-
No products found
because this supplier's products are not listed.
Camilla S. Colding-Christensen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The following antibodies were raised against the indicated peptides derived from Xenopus laevis proteins (New England Peptide now Biosynth): Dbn1 (Ac-CWDSDPVMEEEEEEEEGGGFGESA-OH) ...
-
No products found
because this supplier's products are not listed.
Yingyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
C1-INH protein levels were measured using the SPAplus automated protein analyzer (The Binding Site Group Ltd, Birmingham, UK) – which utilizes turbidimetric measurement to determine protein level – according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Qiyue Ding, et al.,
bioRxiv - Biochemistry 2020
Quote:
Protein thiol redox states were monitored using the -SulfoBiotics- Protein Redox State Monitoring Kit Plus (Catalog # SB12; Dojindo Molecular Technologies). Samples were labeled with the Protein-SHifter Plus in accordance with the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Drake Jensen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... These templates underwent an additional PCR step with Klentaq LA DNA Polymerase (84) (DNA Polymerase Technology; catalog # 110), using a biotinylated primer (Integrated DNA Technologies ...
-
No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
-
No products found
because this supplier's products are not listed.
Delia Onorini, et al.,
bioRxiv - Microbiology 2022
Quote:
... The primary antibody used was a Chlamydiaceae family-specific rabbit polyclonal antibody LPS/MOMP antibody (Cygnus Technologies, Inc., Southport, NC, USA) at a 1:1000 dilution ...
-
No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... and the column was washed with lysis buffer to remove unbound proteins before eluting the proteins with 50 μM biotin (Chem-Impex Int’l) in low salt buffer (20mM HEPES-KOH pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Amplicon pools containing the TSS and barcode regions were generated using plasmid DNA from E.coli or yeast by Micellula DNA Emulsion & Purification (ePCR) Kit (EURx/CHIMERx, 3600) per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
30 µL of a suspension of photocleavable DNA microbeads (60% photocleavable linker DNA microbeads) were pelleted using a C1008-GE myFUGE mini centrifuge (Benchmark Scientific) for 2 min ...
-
No products found
because this supplier's products are not listed.
Joanna M. Cooper, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and IV Fc chimera proteins were produced by Molecular Innovations. His-tagged recombinant human tau variants 2N4R ...
-
No products found
because this supplier's products are not listed.
Minal Engavale, et al.,
bioRxiv - Immunology 2023
Quote:
... Taq DNA polymerase was from Syd Labs (Hopkinton, MA). The anti-human Dnase1L3 rabbit polyclonal antibodies were obtained from Abnova (Cat#H00001776-W01P ...
-
No products found
because this supplier's products are not listed.
Bianca Köhler, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... for siR-DNA fluorescence detection and a photomultiplier (PMT) were applied ...
-
No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
-
No products found
because this supplier's products are not listed.
Andres Tapia del Fierro, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research; BaseClear Lab Products, Leiden, the Netherlands) following the instructions of the manufacturer ...
-
No products found
because this supplier's products are not listed.
J.I. Hoare, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... All cell lines underwent 16 locus STR verification (DNA Diagnostics Centre ...
-
No products found
because this supplier's products are not listed.
Nicholai M. Hensley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Varying volumes of concentrated protein solution and luciferin assay mix (Targeting Systems; prepared to manufacturer’s specifications but with unknown concentration for mammalian cells ...
-
No products found
because this supplier's products are not listed.
MaryAnn Martin, Irene L.G. Newton,
bioRxiv - Microbiology 2023
Quote:
Proteins were separated on 4-20% Tris-Glycine NB precast minigels (NuSep) and transferred to PVDF membrane in Tris-Glycine transfer buffer with 15% methanol at 40v on ice for 3 hours ...
-
No products found
because this supplier's products are not listed.
Aswini Panigrahi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Anti-Sulf-2 monoclonal antibodies (QED Bioscience), Anti-LG3BP monoclonal antibody (Proteintech) ...
-
No products found
because this supplier's products are not listed.
Julia F. Nepper, Yin C. Lin, Douglas B. Weibel,
bioRxiv - Microbiology 2019
Quote:
... Genomic DNA was removed using the ArcticZymes HL-dsDNase (ArcticZymes, Norway), and RNA was reverse transcribed using an Applied Biosystems High Capacity RNA-to-cDNA kit (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Lysate (600 μl) was incubated with 5 μg BAG-1L specific antibody rabbit monoclonal antibody (clone RM310; RevMAb Biosciences) at 4 °C for 16 hours to analyze the specificity of this antibody for its target ...
-
No products found
because this supplier's products are not listed.
Fabian Braun, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... was diluted with antibody diluent (Medac-diagnostica, Germany) 1:1000 and developed using poly-HRP-anti mouse/rabbit IgG (Bright Vision ...
-
No products found
because this supplier's products are not listed.
Robyn M. Barfield, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... The murine anti-maytansine antibody was made by ProMab and validated in-house ...
-
No products found
because this supplier's products are not listed.
M.M. Joglekar, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... VCAN (1:200, Mouse Anti-Versican Antibody 2B1, Seikagaku), and ELN (1:400 ...
-
No products found
because this supplier's products are not listed.
Jack L. Scanlan, et al.,
bioRxiv - Genetics 2020
Quote:
... melanogaster EcKL protein sequences as queries and were annotated in Artemis (Carver et al., 2012). Gene model annotation was performed by reciprocally performing BLASTP and TBLASTN searches (Altschul et al. ...
-
No products found
because this supplier's products are not listed.
Paweł P. Knejski, et al.,
bioRxiv - Biochemistry 2023
Quote:
... purified proteins were diluted to 0.05 mg/mL and applied onto plasma-cleaned (Gatan Solarus) copper grids with a continuous carbon layer (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Elva Vidya, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Two mg of proteins were used in four rounds of rabbit injection (Capralogics, Cambridge Massachusetts) and a total of three bleeds were collected and characterized.
-
No products found
because this supplier's products are not listed.
Roman Franěk, et al.,
bioRxiv - Zoology 2019
Quote:
... The relative DNA content was determined using a CyFlow Ploidy Analyzer (Sysmex Partec GmbH, Germany) against samples from diploid control groups ...
-
No products found
because this supplier's products are not listed.
Venkatesh Mallikarjun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins from cell pellets were solubilised by bead-beating using six 1.6 mm steel beads (Next Advance) at 4 °C in 30 µL 1.1% (w/v ...
-
No products found
because this supplier's products are not listed.
Magdalena Kordon, et al.,
bioRxiv - Cell Biology 2019
Quote:
After cell fixation BrdU was incorporated into DNA ends using terminal deoxynucleotidyl transferase (TdT) (Phoenix Flow Systems, AU: 1001) and detection and fluorescence enhancement was achieved by applying a procedure described in detail in Fig ...
-
No products found
because this supplier's products are not listed.
Kristine Farmen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The primary antibody used was pneumococcal serotype 4 anti-capsule (SSI Diagnostica, 1:250), secondary antibody used was Alexa Fluor 620 (1:1000) ...
-
No products found
because this supplier's products are not listed.
Bernardo Papini Gabiatti, et al.,
bioRxiv - Biochemistry 2024
Quote:
... mCherry with the ET-mCherry-Texas-Red filter and DNA with the DAPI filter (Chroma Technology CORP, Bellows Falls, VT). Z-stacks (75 slices ...